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| There are 479 papers published in subject: since this site started. | |||
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| 1. Research progress of related methods for controlling targeted protein level | |||
| MIn Gao,ChongSheng He | |||
Biology 10 August 2021
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| Show/Hide Abstract | Cite this paper︱Full-text: PDF (354K B) | |||
| Abstract:In this paper, we reviewed the progress of target protein degradation methods, with special attention to the use of plant hormones to stimulate the degradation. Protein is the key player of many biological processes, interfering the protein level precisely is both important for basic research and clinical application. There are many ways to control the protein level artificially in different levels such as CRISPR-Cas9, RNAi and so on. These methods show different limitations, for example, CRISPR-Cas9 is irreversible, RNAi can cause off-target effect. Targeted protein degradation is one of the ways to regulate the protein level in a post-translational level, it can affect the protein level very fast and reversibly. To achieve targeted protein degradation, different methods have been developed such as small-molecule induced degradation, photo-induced degradation, auxin induced degradation and so on. The core principle is to degrade the target protein in a controllable manner. | |||
| TO cite this article:MIn Gao,ChongSheng He. Research progress of related methods for controlling targeted protein level[OL].[10 August 2021] http://en.paper.edu.cn/en_releasepaper/content/4755399 | |||
| 2. A 110 nt fragment in the 5\'UTR of STAT2 that determines IRES activity and influences cell proliferation | |||
| Zhou Kantian,Liao Zili,Zhu Ruiyu | |||
Biology 30 March 2021
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| Show/Hide Abstract | Cite this paper︱Full-text: PDF (641K B) | |||
| Abstract:As a unique member of the STAT (Signal Transducer and Activator of Transcription) proteins family, STAT2 cannot recognize DNA target sites as homodimers like the others, yet it plays a critical role in type I interferon (IFN-I) signaling and many other signaling pathways. It was reported that STAT2 could help cells to cope with stress conditions by promoting antiviral responses in normal cells or drug resistance in carcinoma cells. As IRES (Internal Ribosome Entry Sites) were known to help promoting survival of cells under stress, together with the discovery that the 5\'UTR of STAT2 could form stable stem-loop secondary structures with a length of 203 nt, suggesting that there might be IRES activity. To testify this speculation, bicistronic reporter assay was designed as the main method to find out whether the 5\'-UTR of STAT2 harbors an IRES element or not. During the process, the IRES activity was confirmed, followed by the discovery that IRES active central domain was located at nt 33-142 in the 5\'UTR of STAT2. Afterwards, CRISPR/Cas9 techniques were utilized to construct a knockout cell line with the deletion of sequence between nt 65-129 in 5\'UTR. And it was discovered that knockout cells grew slightly faster than those wild type ones, which meant that the knocked-out fragment had an impact on normal cell proliferation. The exact mechanism behind this result remains to be further investigated. | |||
| TO cite this article:Zhou Kantian,Liao Zili,Zhu Ruiyu. A 110 nt fragment in the 5\'UTR of STAT2 that determines IRES activity and influences cell proliferation[OL].[30 March 2021] http://en.paper.edu.cn/en_releasepaper/content/4754243 | |||
| 3. Enhancement of cytoplasmic solubility of heparinase I from Flavobacterium heparinum in Escherichia coli by adding charged tag and co-expression of molecular chaperonin from hyperthermophilic archaea. | |||
| Guo Yu,Li Huichao,Zhang Hao,Fujiwara Shinsuke,Gao Le | |||
Biology 01 February 2021
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| Show/Hide Abstract | Cite this paper︱Full-text: PDF (561K B) | |||
| Abstract:Heparinase is important in the heparin production and application. The most studied heparinase I is HepA which is secreted by Flavobacterium heparinum. Since HepA was hard to obtain as a soluble form in Escherichia coli, many works were tried to increase cytoplasmic solubility of HepA by adding various protein tags to the HepA terminus. Here we designed a positively charged hexapeptide tag (RGRRGG) which was expected to have enhanced affinity to a chaperonin CpkA from hyperthermophilic archaeaon Thermococcus kodakarensis. HepA was first fused with RGRRGG and expressed by plasmid pET21a. Effect of tag addition on cytoplasmic soluble amount of HepA in E. coli were examined, showing that the amount of the tag fused HepA(HepA\') was boosted in comparison with tag-less original one. HepA\' was next co-expressed with a CpkA by another compatible plasmid pACYCDuet-1. By CpkA co-expression, insoluble amount of HepA\' was decreased and soluble amount of HepA\' was increased and reached to 40% of total expressed amount of HepA\'. The specific activity (126 IU/mg) was also comparable to the native HepA (130 IU/mg). The present data indicated that tag-charged HepA was efficiently obtained as an active form by co-expressing CpkA. (10 Points, Times New Roman) | |||
| TO cite this article:Guo Yu,Li Huichao,Zhang Hao, et al. Enhancement of cytoplasmic solubility of heparinase I from Flavobacterium heparinum in Escherichia coli by adding charged tag and co-expression of molecular chaperonin from hyperthermophilic archaea.[OL].[ 1 February 2021] http://en.paper.edu.cn/en_releasepaper/content/4753657 | |||
| 4. Characterization and isolation of estradiol degrading bacteria Acinetobacter sp. (7-1) Isolated from Changchun, China. | |||
| YU Jian,ZHAO yanyang,HOU yue | |||
| Biology 15 December 2020
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| Show/Hide Abstract | Cite this paper︱Full-text: PDF (843K B) | |||
| Abstract:17β- Estradiol, as a major environmental estrogen, is a serious threat to human health and ecological security. The MS medium containing 0.5 mM estradiol as the sole carbon source was used to isolate a new Gram-negative bacteria from the fishing pond. This screening strain can use as the sole carbon source and energy source such as estradiol and testosterone. After 16S rRNA analysis, the bacteria belong to Acinetobacter sp with 99.71% identity. The strain was Gram-negative bacilli, Spherical, Blunt round ends, Scattered or arranged in pairs, No bud, No flagella. Size 2.126μm×0.753μm; Conditions for optimum degradation of estradiol were 27℃, 150 rpm, and substrate concentration was 1 mM/L, pH7.5 and 1% inoculum. The highest degradation rate, the degradation rate can reach 85%. The strain could grow normally in 5 μg/mL auromycin, but not in 100 μg/mL ampicillin,50 μg/mL kanamycin,10 μg/mL streptomycin. The degradation rate of 17β- estradiol was 81.4%, that of testosterone was 64.6%, that of progesterone was 44.6%, and that of 17α- ethinyl estradiol was 62.3% by high performance liquid chromatography. | |||
| TO cite this article:YU Jian,ZHAO yanyang,HOU yue. Characterization and isolation of estradiol degrading bacteria Acinetobacter sp. (7-1) Isolated from Changchun, China.[OL].[15 December 2020] http://en.paper.edu.cn/en_releasepaper/content/4753226 | |||
| 5. Tyrosine hydroxylase (TH) upstream-inhibited UPF3 regulator of nonsense transcripts homolog A (UPF3A) subnetwork for learning in human left hemisphere|Prostate via nucleus to mitochondrion to cytosol RNA binding | |||
| Xi Ruipeng,HUANG Juxiang,WANG Lin | |||
| Biology 14 September 2020
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| Show/Hide Abstract | Cite this paper︱Full-text: PDF (310K B) | |||
| Abstract:High tyrosine hydroxylase (TH) upstream-inhibited UPF3 regulator of nonsense transcripts homolog A (yeast) (UPF3A) molecular subnetwork was constructed, including upstream hypothetical LOC400642 (LOC400642), retinoblastoma binding protein 6 (RBBP6), ubiquitin protein ligase E3 component n-recognin 5 (UBR5); downstream chromosome 10 open reading frame 10 (C10orf10), sulfotransferase family 1A member 2 (SULT1A2) reported relation with learning in human left hemisphere. The common biology process of TH upstream-inhibited UPF3A subnetwork was identified by DAVID, containing upstream RBBP6, upstream UBR5, downstream SULT1A2, second-core UPF3A, first-core TH as protein binding; upstream RBBP6, upstream UBR5 as ubiquitin protein transferase activity, zinc ion binding, ligase activity, cellular response to DNA damage stimulus, protein ubiquitination involved in ubiquitin dependent protein catabolic process; upstream UBR5, second-core UPF3A as RNA binding; downstream SULT1A2, first-core TH as small molecule metabolic process; The common cellular component of upstream RBBP6, upstream UBR5, second-core UPF3A, first-core TH as cytoplasm; upstream RBBP6, upstream UBR5, second-core UPF3A as nucleoplasm; upstream UBR5, second-core UPF3A, first-core TH as nucleus; downstream SULT1A2, second-core UPF3A, first-core TH as cytosol; downstream C10orf10, first-core TH as mitochondrion; The common tissue distributions as Prostate_3rd maybe exist the same pattern with human left hemisphere. We propose and mutual positively verify tyrosine hydroxylase (TH) upstream-inhibited UPF3 regulator of nonsense transcripts homolog A (yeast) (UPF3A) subnetwork for learning in human left hemisphere|Prostate via nucleus to mitochondrion to cytosol RNA binding. | |||
| TO cite this article:Xi Ruipeng,HUANG Juxiang,WANG Lin. Tyrosine hydroxylase (TH) upstream-inhibited UPF3 regulator of nonsense transcripts homolog A (UPF3A) subnetwork for learning in human left hemisphere|Prostate via nucleus to mitochondrion to cytosol RNA binding[OL].[14 September 2020] http://en.paper.edu.cn/en_releasepaper/content/4752808 | |||
| 6. Observe the difference of COVID-19 variation in different regions from the type and number of base mutations | |||
| LIU Jian-Zhong, Zheng Jeffrey | |||
| Biology 06 May 2020 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (618K B) | |||
| Abstract:Collecting Covid-19 genomes from three regions: Shanghai-China, Tbilisi-Georgia and Sydney-Australia, five similar genomes are selected from each region for research in this paper. Applying ”Datum gene sequence” method proposed,our results are shown that variation is immense in Sydney-Australia region, then variation is shown in the second in Tbilisi-Georgia region and it has a minimal value in Shanghai-China region respectively. | |||
| TO cite this article:LIU Jian-Zhong, Zheng Jeffrey. Observe the difference of COVID-19 variation in different regions from the type and number of base mutations[OL].[ 6 May 2020] http://en.paper.edu.cn/en_releasepaper/content/4751960 | |||
| 7. A review: Inhibitors and activators for paraoxonase 1 | |||
| Li Yan,Tang Meiling,Chen Gang,Mu Xiaojing,Huang Haiming | |||
| Biology 23 April 2020
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| Show/Hide Abstract | Cite this paper︱Full-text: PDF (782K B) | |||
| Abstract:Paraoxonase 1 (PON1) is a calcium-dependent hydrolase with many physiological functions. The decrease of PON1 activity is related to many diseases. The research about PON1 is a hot topic, and its activators and inhibitors are expected to help in our diets and in choose of drugs. The papers about PON1 activators and inhibitors were collected and classified. The PON1 activators and inhibitors are grouped into four categories: in-vivo activators, in-vitro activators, in-vivo inhibitors and in-vitro inhibitors. For each activator or inhibitor, the activating effect or inhibiting effect along with the methods used was reviewed. Up to now, we found from literatures that there were 16 samples demonstrating activating effects on PON1 in-vivo and 5 compounds showed in-vitro activation. Of the 16 in-vivo activators there were 4 plant extracts and 12 pure components. Seven in-vivo inhibitors and 71 in-vitro inhibitors have been reported in publications. Additionally, smoking, drinking and treatment with exogenous gonadotropins induced decrease of PON1 activity. The summary of the activators and inhibitors are beneficial to reveal the structure-activity relationship, and also it provides convenience to look up the activating or inhibiting effects of the compounds on PON1 at a glance. | |||
| TO cite this article:Li Yan,Tang Meiling,Chen Gang, et al. A review: Inhibitors and activators for paraoxonase 1[OL].[23 April 2020] http://en.paper.edu.cn/en_releasepaper/content/4751765 | |||
| 8. A 69 nt segment in the 5'UTR regulates translation of FOXO3a under stress conditions | |||
| Zhang Xuyan,Zhou Kantian,Zhu Ruiyu | |||
| Biology 31 March 2020
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| Show/Hide Abstract | Cite this paper︱Full-text: PDF (1039K B) | |||
| Abstract:Fork head box O3 (FOXO3a) is a transcription factor that mediates various fundamental cellular processes, including cell cycle progression, proliferation, DNA damage and apoptosis. This study showed that the 5\' untranslated region (5\'UTR) of FOXO3a mRNA contains an internal ribosome entry site (IRES). After mapping the 5\'UTR of FOXO3a mRNA, we found that a 69 nt sequence located between 175-243 was the key segment for the overall IRES activity. Further, we generated a knockout cell line of this 69 nt segment using CRISPR technology. During serum-starvation and ADM drug stress, the deletion of this 69 nt segment could inhibit the expression of FOXO3a to some extent in the knockout cells. These results strongly suggested that the 69 nt segment is essential for self-regulation of FOXO3a under stress conditions, and could be a potential target for the regulation of FOXO3a. | |||
| TO cite this article:Zhang Xuyan,Zhou Kantian,Zhu Ruiyu. A 69 nt segment in the 5'UTR regulates translation of FOXO3a under stress conditions[OL].[31 March 2020] http://en.paper.edu.cn/en_releasepaper/content/4751435 | |||
| 9. TH upstream-inhibited UBR5 subnetwork for learning in human left hemisphere|Prostate via nucleus to cytoplasm protein binding | |||
| Wang Yuanzhen,WANG Lin,HUANG Juxiang | |||
| Biology 27 December 2019 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (289K B) | |||
| Abstract:High tyrosine hydroxylase (TH) upstream-inhibited ubiquitin protein ligase E3 component n-recognin 5 (UBR5) molecular subnetwork was constructed, including feedback sulfotransferase family 1A member 2 (SULT1A2); downstream chromosome 10 open reading frame 10 (C10orf10), heterogeneous nuclear ribonucleoprotein H3 (HNRPH3), UPF3 regulator of nonsense transcripts homolog A (yeast) (UPF3A) reported relation with learning in human left hemisphere. The common biology process of TH upstream-inhibited UBR5 subnetwork was identified by DAVID, containing feedback SULT1A2, downstream HNRPH3, downstream UPF3A, second-core UBR5, first-core TH as protein binding; downstream HNRPH3, downstream UPF3A, second-core UBR5 as RNA binding; feedback SULT1A2, first-core TH as small molecule metabolic process; downstream HNRPH3, downstream UPF3A as nucleotide binding; The common cellular component of downstream HNRPH3, downstream UPF3A, second-core UBR5, first-core TH as nucleus; feedback SULT1A2, downstream UPF3A, first-core TH as cytosol; downstream HNRPH3, downstream UPF3A, second-core UBR5 as nucleoplasm; downstream UPF3A, second-core UBR5, first-core TH as cytoplasm; downstream C10orf10, first-core TH as mitochondrion; The common tissue distributions as Prostate_3rd maybe exist the same pattern with human left hemisphere. We propose and mutual positively verify tyrosine hydroxylase (TH) upstream-inhibited ubiquitin protein ligase E3 component n-recognin 5 (UBR5) subnetwork for learning in human left hemisphere|Prostate via nucleus to cytoplasm protein binding. | |||
| TO cite this article:Wang Yuanzhen,WANG Lin,HUANG Juxiang. TH upstream-inhibited UBR5 subnetwork for learning in human left hemisphere|Prostate via nucleus to cytoplasm protein binding[OL].[27 December 2019] http://en.paper.edu.cn/en_releasepaper/content/4750367 | |||
| 10. TH upstream-inhibited DCI subnetwork for learning in human left hemisphere|Tonsil via nucleo to cytoplasm poly(A) RNA binding | |||
| YANG Kaitong,WANG Lin,HUANG Juxiang | |||
| Biology 24 December 2019
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| Show/Hide Abstract | Cite this paper︱Full-text: PDF (464K B) | |||
| Abstract:High tyrosine hydroxylase (TH) upstream-inhibited enoyl-CoA delta isomerase 1 (DCI) molecular subnetwork was constructed, including upstream NCK adaptor protein 2 (NCK2), retinoblastoma binding protein 6 (RBBP6), sulfotransferase family 1A member 2 (SULT1A2); downstream chromosome 10 open reading frame 10 (C10orf10), forkhead box N3 (FOXN3_2), poly (rC) binding protein 2 (PCBP2_2) reported relation with learning in human left hemisphere. The common biology process of TH upstream-inhibited DCI subnetwork was identified by DAVID, containing upstream NCK2, upstream RBBP6, upstream SULT1A2, downstream FOXN3_2, downstream PCBP2_2, first-core TH as protein binding; upstream SULT1A2, second-core DCI, first-core TH as small molecule metabolic process; upstream RBBP6, downstream PCBP2_2 as poly(A) RNA binding; downstream PCBP2_2, first-core TH as enzyme binding; The common cellular component of upstream NCK2, upstream RBBP6, downstream PCBP2_2, first-core TH as cytoplasm; upstream NCK2, upstream SULT1A2, downstream PCBP2_2, first-core TH as cytosol; downstream C10orf10, second-core DCI, first-core TH as mitochondrion; downstream FOXN3_2, downstream PCBP2_2, first-core TH as nucleus; upstream RBBP6, downstream PCBP2_2 as nucleoplasm; downstream PCBP2_2, second-core DCI as extracellular exosome; The common tissue distributions as Tonsil_3rd maybe exist the same pattern with human left hemisphere. We propose and mutual positively verify tyrosine hydroxylase (TH) upstream-inhibited enoyl-CoA delta isomerase 1 (DCI) subnetwork for learning in human left hemisphere|Tonsil via nucleo to cytoplasm poly(A) RNA binding. | |||
| TO cite this article:YANG Kaitong,WANG Lin,HUANG Juxiang. TH upstream-inhibited DCI subnetwork for learning in human left hemisphere|Tonsil via nucleo to cytoplasm poly(A) RNA binding[OL].[24 December 2019] http://en.paper.edu.cn/en_releasepaper/content/4750327 | |||
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