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1. Study on the renaturation process of epidermal growth factor-like protein and its therapeutic effect on neuroinflammation | |||
Yang Jingru,He Huahong,He Huafeng,Wang Huaqian | |||
Biology 25 March 2023 | |||
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Abstract:Epidermal growth factor (EGF) plays an important role in improving the inflammatory response. In this study, cholera toxin A2 subunit, cell-penetrating peptide trans-transcription activator (TAT) and EGF were fused and recombinant protein EGF-CTA2-TAT were expressed by Escherichia coli. Two methods of renaturation, affinity chromatography and dialysis, were selected to renature the protein. The renaturation efficiency of these two methods were compared. Activities of recombinant protein on promoting BALB/c 3T3 cells proliferation in vitro were also studied. After that, mice model of neuroinflammation was established by using the Lipopolysaccharide (LPS) intraperitoneal injection. The purified and renatured recombinant EGF-like protein was administered intranasally for neuroinflammation treatment. The Morris water maze (MWM) test was used to evaluate the therapeutic effects. Our results showed that recombinant EGF-like protein had a therapeutic effect on LPS-induced neuroinflammation in mice, and could remarkably improve the learning and memory ability of mice. The recombinant EGF-like protein renatured and purified by affinity chromatography in one step shows the advantages of simple process, high yield, excellent activity and low cost, providing a reference for mass production of such active proteins. | |||
TO cite this article:Yang Jingru,He Huahong,He Huafeng, et al. Study on the renaturation process of epidermal growth factor-like protein and its therapeutic effect on neuroinflammation[OL].[25 March 2023] http://en.paper.edu.cn/en_releasepaper/content/4759631 |
2. The effect of centrally administered apelin-13 on interdigestive gastric motility in conscious rats | |||
LV Shuangyu,QIN Yaojun,WANG Ningbo,YANG Yanjie,CHEN Qiang | |||
Biology 02 August 2012 | |||
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Abstract:Apelin, a novel neoropeptide, was identified as the endogenous ligand for APJ receptor. The present study was designed to explore the effect apelin-13 on interdigestive gastric motility in conscious rats. Apelin-13 was synthesized by the solid-phase peptide synthesis method. After recovery from the operation of intracerebroventricular (i.c.v.) cannula implantation, the strain gauge transducers were implanted on the serosal surface of the antrum in Wistar rats. The migrating motor complex (MMC) was instantly recorded by the Power-Lab recording system. The results show that, comparing with control group (5.17±0.93), the frequency of the phase Ⅲ-like activity (c/h) of the 10 nmol apelin-13 group (7.67±1.33) and 30 nmol apelin-13 group (8.58±1.66) were both increased, showing a statistically significant difference (P < 0.01, P < 0.01, respectively). However, frequency of the phase Ⅲ-like activity of 3 nmol apelin-13 group (6.83±0.98) had no significant difference (P > 0.05). Comparing with control group (97.41±16.88), the %motor index (MI) of the 10 nmol apelin-13 group (143.93±26.27) and 30 nmol apelin-13 group (155.09±24.02) were both increased, indicating a statistically obvious difference (P < 0.01, P < 0.01, respectively). However, the %MI of 3 nmol apelin-13 group (120.14±3.00) showed no significant difference (P > 0.05). Our study first indicates that i.c.v. injection of apelin-13 increased the interdigestive antrum MMC in conscious rats, which has an important physiological significance for further study the modulatory effect of apelin-13 on the digestive tract motility. | |||
TO cite this article:LV Shuangyu,QIN Yaojun,WANG Ningbo, et al. The effect of centrally administered apelin-13 on interdigestive gastric motility in conscious rats[OL].[ 2 August 2012] http://en.paper.edu.cn/en_releasepaper/content/4485939 |
3. Does FcγRIII (CD16) mediate modified C-reactive protein-induced endothelial cells dysfunction? | |||
Ji Shangrong | |||
Biology 10 September 2008 | |||
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Abstract:Atherosclerosis and its complications, in particular acute coronary heart disease (ACHD) is the single leading cause of morbidity and mortality world wide. Among the clinically useful risk markers for development of ACHD, the classical acute-phase reactant C-reactive protein (CRP) has received considerable attention. Accumulating evidence indicates that CRP is not merely a corollary marker but rather an actual mediator/modulator of the inflammation that contributes to ACHD. | |||
TO cite this article:Ji Shangrong. Does FcγRIII (CD16) mediate modified C-reactive protein-induced endothelial cells dysfunction?[OL].[10 September 2008] http://en.paper.edu.cn/en_releasepaper/content/23936 |
4. Structural and dynamic properties of a new highly amyloidogenic chicken cystatin mutant I108T | |||
Liu Yuan ,Hu Bingjie ,Wan Dongmei ,Wang Yaofeng ,Yu Yuanyuan ,Song Youtao | |||
Biology 15 April 2008 | |||
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Abstract:Chicken cystatin mutant I108T was shown many amyloid characters compared with amyloidogenic mutant I66Q and wild type chicken cystatin in our previous biochemical experiments. In this study, 10-ns molecular dynamics simulations of the I108T, I66Q mutants and wild-type chicken cystatins were performed to further investigate the amyloidogenic capacity of I108T mutant. Experimental evidence shows that I108T mutant has an “expand” tendency to destabilize the whole protein and facilitates the aggregation process through four ways: (1) alter the hydrophobicity and expose large hydrophobic surface area to the solvent; (2) decrease the α-helix content and convert it into unordered structure; (3) weaken hydrogen between β2- and β3-strand; (4) weaken salt bridge interactions. Our results strongly suggest that the I108T mutant, which exhibits large structural fluctuations compared with wild-type chicken cystatin, is a highly amyloidogenic form of chicken cystatin and shares many similar structural and dynamic features with amyloidogenic I66Q mutant. | |||
TO cite this article:Liu Yuan ,Hu Bingjie ,Wan Dongmei , et al. Structural and dynamic properties of a new highly amyloidogenic chicken cystatin mutant I108T[OL].[15 April 2008] http://en.paper.edu.cn/en_releasepaper/content/20513 |
5. Studies In Vitro and In Vivo of Pharmacological Activities of PFR(Tic)amide | |||
Fang Quan ,He Feng ,Wang Yiqing ,Chen Qiang ,Wang Rui | |||
Biology 30 November 2006 | |||
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Abstract:Neuropeptide FF (NPFF) belongs to a neuropeptide family including two precursors (pro-NPFFA and pro-NPFFB) and two receptors (NPFF1 and NPFF2). The NPFF analogue PFR(Tic)amide was originally found as an putative antagonist on NPFF receptors because of its depressor response, while it was recently shown to be a “super-agonist” on NPFF1 and NPFF2 receptors in vitro. To further evaluate its pharmacological profiles, in the present work, PFR(Tic)amide were synthesized and investigated to address their potencies and efficacies in a series of assays. (1) In the isolated mouse colon bioassay, it evoked significant colonic contractions at a high dose of 50 μM, which were attenuated by pretreatment with BIBP3226; (2) PFR(Tic)amide (10 ~ 30 nmol/mouse) injected into the third ventricle dose-dependently induced marked hypothermia in a manner similar to NPFF. Our results suggest that PFR(Tic)amide acts as an NPFF agonist in vitro and in vivo. Furthermore, it shows much higher potency in thermoregulatory test, compared to NPFF. | |||
TO cite this article:Fang Quan ,He Feng ,Wang Yiqing , et al. Studies In Vitro and In Vivo of Pharmacological Activities of PFR(Tic)amide[OL].[30 November 2006] http://en.paper.edu.cn/en_releasepaper/content/10078 |
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