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1. N-glycan-defective breast cancer cellsinduce a phenotypic switch inpolarization of bone marrow-derivedmacrophages | |||
Chen Haidan ,huili CAI,LiDongqing | |||
Basic Medicine 27 February 2013 | |||
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Abstract:Purpose: To investigate the e!ect of N-glycan-defective mammary adenocarcinoma cells on the polarization of macrophages. Methods: N-glycan-defective breast cancer cells (MA782 cells) were prepared by swainsonine(SW) treatment and the cytotoxicity of SW to MA782 cells was evaluated using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. "e Nglycan-defective MA782 cells were co-cultured with bone marrow-derived macrophages (BMDMs) for 48 h in vitro, and then the BMDMs and the co-cultured supernatant were analyzed for macrophage phenotypic using FQRT-PCR, FCM and ELISA. Results: SW-treated MA782 cells expressed defective N-glycan on the cell surface in a dose-dependent manner (*p<0.05). MTT assays showed that neither the 1 ug/mL nor 5ug/ mL SW treatments showed signi$cant inhibition of MA782 cell growth in vitro. "e expression of iNOS and agr-1 in the 5 #g/mL SW-treated group were 4.75-fold higher and 3.7-fold lower than that in the untreated group, respectively (*p<0.05). Mean %uorescence intensity of CD16/32 expressed in the cells treated with 5 #g/mL SW was signi$cantly higher in comparison with the untreated group (65 vs. 7, *p<0.05), though the percentage of CD16/32-positive cells were not signi$cantly di!erent. Furthermore, the expression of CD206 and dectin-1 in the 5 #g/mL SW-treated group was signi$cantly decreased (3.1±0.3% and 4.1±1.1%, respectively) in comparison with the untreated group (40±3% and 8.9±1.2%, respectively, both p<0.05). In addition, the 5 #g/mL SW-treated group secreted more TNF-alpha (350 ±25 pg/mL) and less IL-10 (89±7.2 pg/mL) than the untreated group (80 ±3 pg/mL and 150 ±10 pg/mL, respectively, both p<0.05). Conclusion: N-glycan-defective MA782 cells can induce the di!erentiation of BMDM into proin%ammatory M1 macrophages in vitro. | |||
TO cite this article:Chen Haidan ,huili CAI,LiDongqing. N-glycan-defective breast cancer cellsinduce a phenotypic switch inpolarization of bone marrow-derivedmacrophages[OL].[27 February 2013] http://en.paper.edu.cn/en_releasepaper/content/4522243 |
2. Effect of consumption of olestra or regular triglyceride cake on gastrointestinal symptoms | |||
DOU Yujing,LIU Jing,ZHU Jing,LIU Yuanfa,WANG Xingguo | |||
Basic Medicine 05 December 2012 | |||
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Abstract:Background: olestra is a new type of fat substitutes. Because it is not absorbed, it may cause gastrointestinal Symptoms when consumed in large amounts. Objective: The aim was using cake as the carrier of olestra to compare the effect of gastrointestinal symptoms in people when consumed in different amounts. Methods: A randomized, double-blind, placebo-controlled trial compared the effects of olestra cake (11g/day or 22g/day in cake) with those of regular butter cake on gastrointestinal symptoms. The study lasted 9 days: 3days baseline and 6 days treatment. The gastrointestinal symptoms and their impact on daily activities were collected by telephone interviewers and recorded in a daily record. Results: Gastrointestinal symptoms, such as diarrhea, abdominal pain, flatulence, nausea, loose stools, more frequent bowel movement and so on, were observed in our study. The incidence of diarrhea (liquid/watery stools, bowel movement frequency > 3/day) which people and researchers paid more attention to was only 0.79% when one participant consumed 11g olestra. Of 63 evaluable subjects in the olestra cake group (22g/day) or in the butter cake group, incidence of GI adverse events was 1.6% lower than the incidence consumed 11g/day (6.4%). There was not significant difference between different intake doses in the incidence of gastrointestinal adverse events by chi-square (p=0.371>0.05). The incidence of gastrointestinal function change had a growing trend with the increase of olestra intake. But it is not significant by chi-square (p=0.541>0.05). Gastrointestinal symptoms, such as diarrhea, abdominal pain, flatulence, nausea, loose stools, mainly occurred in the first day after eating up cake, while more frequent bowel movement mainly occurred in the first and second day. The effect of sex and age on the incidence of gastrointestinal symptoms was not significant. Conclusions: This study demonstrates that consumption of olestra is not associated with increased incidence or severity of gastrointestinal symptoms. So it is safe and acceptable that olestra, as fat replacer, is applied in food. | |||
TO cite this article:DOU Yujing,LIU Jing,ZHU Jing, et al. Effect of consumption of olestra or regular triglyceride cake on gastrointestinal symptoms[OL].[ 5 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4499614 |
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