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1. The structure and functions of SARS-CoV-2 nucleocapsid protein | |||
SONG Aling,GE Xingyi,QIU Ye | |||
Biology 18 April 2023 | |||
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Abstract:Severe Acute respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the third highly pathogenic human coronavirus after SARS-CoV and MERS-CoV. SARS-CoV-2 can not only cause flu-like symptoms such as cough, fever, fatigue and sore limbs, but also lead to complications such as pulmonary fibrosis, acute kidney injury and neurodegenerative diseases in partial patients. As the most abundant protein in SARS-CoV-2, N protein participates in the packaging and transcription of viral RNA, and moreover plays important roles in affecting host cell homeostasis. Previous researches demostrated that N protein triggers activation of the Smad3 signaling pathway through the p21‐ dependent G1 cell cycle arrest mechanism which leads to cell death, N protein also inhibits GSDMD-mediated pyroptosis to inhibit the release of IL-1β. N protein regulating host cell antiviral immune response through NF-κB, JAK-STAT and other signaling pathways has been revealed. In addition, studies have proven that N protein is associated with many complications, such as Parkinson\'s disease, pulmonary fibrosis and so on. In this review, we describe the known structure of SARS-CoV-2 N protein, its role in packaging viral RNA and effects on host cell homeostasis. | |||
TO cite this article:SONG Aling,GE Xingyi,QIU Ye. The structure and functions of SARS-CoV-2 nucleocapsid protein[OL].[18 April 2023] http://en.paper.edu.cn/en_releasepaper/content/4760370 |
2. 河南地区猪札幌病毒的遗传多样性研究 | |||
Quan Shen,Xiaochun Wang,Rong Fu,Xin Geng,Chengcheng Tao,Zeyu Li,Li Wang | |||
Biology 30 November 2015 | |||
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Abstract:Sapoviruses (SaVs) belong to the family Caliciviridae and are related to gastroenteritis of humans and animals. These agents have been reported from several countries of the world. In this study, A total of 169 stool samples from pigs were collected from 4 pig farms located in Henan Province in central China from January to February 2015. Specimens were tested by reverse transcription polymerase chain reaction (RT-PCR) using the primers p289/p290 designed to detect the polymerase gene of caliciviruses, including both SaVs and noroviruses (NoV). Results showed that three of the four farms wre positive for SaVs and the positive rates were 12.8% 15.6%, and7.1%, respectively. Overall, fiften of 169 (8.9%) stool samples were positive for SaVs. Ten of distinct SaVs sequences were confrimed by sequencing. Phylogenetic analysis based on the partial sequences of the RNA-dependent RNA polymerase (RdRp) gene indicated that these SaVs were divided into five distinct genogroup, GIII, GVII, GVIII, and two potential new genogroup GIX and GX. Four strains belonged to GIII, three stains belonged to GVII, and one belonged to GVIII, GIX or GX, respectivily. No NoV infection were detected in the current study. To the best of our knowledge, this is the first report that GVIII, GIX and GX SaVs infection detected in Chian. Furthermore, the high genetic variability and prevalence of SaVs infection provides evidence that different genogroups of SaVs are circulating in Chinese pig herds. | |||
TO cite this article:Quan Shen,Xiaochun Wang,Rong Fu, et al. 河南地区猪札幌病毒的遗传多样性研究[OL].[30 November 2015] http://en.paper.edu.cn/en_releasepaper/content/4664930 |
3. A novel potexvirus, strawberry mild yellow edge-associated virus 1, identified from strawberry by deep sequencing | |||
AKINYEMI A. Ibukun,QI Shuishui,CHANG Zhaoxia,WU Qingfa | |||
Biology 19 November 2015 | |||
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Abstract:Deep sequencing of diseased Strawberry plants samples from Dangshan, Ahui Province of China resulted in the identification of a new virus genome resembling Potexvirus in sequence and genome structure. A total of 14 contigs were assembled from the clean reads and the gaps in between were bridged to get a genome length of 4177 nucleotides. Phylogenetic analysis suggests that the assembled virus sequence is distantly related to Strawberry Mild Yellow Edge Virus (SMYEV) and can be considered as a new member of the genus Potexvirus, Family Alphaflexiviridae. The virus is tentatively named strawberry mild yellow edge-associated virus 1 (SMYEaV1). | |||
TO cite this article:AKINYEMI A. Ibukun,QI Shuishui,CHANG Zhaoxia, et al. A novel potexvirus, strawberry mild yellow edge-associated virus 1, identified from strawberry by deep sequencing[OL].[19 November 2015] http://en.paper.edu.cn/en_releasepaper/content/4662998 |
4. Genomic dsRNA of Grass carp reovirus protected from both the trigger and effector nucleases of RNA interference pathway during viral replication | |||
Guo Shuai,Lu Liqun | |||
Biology 28 March 2011 | |||
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Abstract:Objective: The present study aimed to investigate the effect of GCRV replication on Dicer-initiated RNAi pathway of host cell. Methods: The pEGFP-N1 was transfected into Grass carp kidney cells (CIK) with either the synthetic EGFP-speific siRNA or in intro transcribed EGFP-specific dsRNA to signify the functional gene silencing pathway in CIK cell line. Then, CIK cells were infected with GCRV and the steady-state transcription levels of Dicer were monitored during viral replication cycle using real-time quantitative PCR (qRT-PCR). The effect of GCRV replication on RNA interference pathway was further studied by investigating the silencing of egfp gene by egfp-siRNA in GCRV-infected CIK cells. Results: both egfp-siRNA and egfp-dsRNA could silence the egfp reporter gene in uninfected CIK cells. In GCRV-infected cells, replication of GCRV correlated with the increased transcription level of Dicer gene while resulted in the loss function of egfp-siRNA on silencing reporter egfp gene. Conclusion: Replication of GCRV augmented the transcription of Dicer gene but still antagonized the classical RNAi pathway of host cells. Suppression of the siRNA-induced RNAi by GCRV replication suggested that GCRV could inhibit the effector protein of RNAi pathway besides the insensitivity of its dsRNA genome to Dicer nuclease, the RNAi- trigger nuclease. | |||
TO cite this article:Guo Shuai,Lu Liqun. Genomic dsRNA of Grass carp reovirus protected from both the trigger and effector nucleases of RNA interference pathway during viral replication[OL].[28 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4418341 |
5. Vaccine potential analysis of Grass carp reovirus capsid protein VP7 through in vitro microneutralization assay | |||
Yang Qian,Lu Liqun | |||
Biology 28 March 2011 | |||
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Abstract:In this paper, the Glutathione S-transferase (GST) fusion protein expression vector pGEX-4T-3 was employed to clone and expression of GCRV outer capsid gene vp7, which was amplified by RT - PCR from infected Grass carp. The recombinant GST-fusion protein rVP7 was induced by 1mM IPTG in Dh5αand confirmed by SDS-PAGE and Western blot assays using both anti-GST-tag and anti-VP7 monoclonal antisera. An expected 52-kDa rVP7 was highly expressed, and was mainly exhibited in the formation of the inclusion body. After purification, rVP7 was intraperitoneally injected to the experimental mice to produce anti-rVP7 polyclonal serum. In vitro microneutralization assay indicated that polyclonal antibody against rVP7 could neutralize GCRV, and suggested that rVP7 had the potential to be used as subunit vaccine against GCRV infection. The present study paved the way for further characterization of the immunogenicity of viral outer capsid protein VP7 in grass carp Ctenopharyngodon idellus and could be based to develop antibody or antigen detection assays for GCRV pathogen. | |||
TO cite this article:Yang Qian,Lu Liqun. Vaccine potential analysis of Grass carp reovirus capsid protein VP7 through in vitro microneutralization assay[OL].[28 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4418350 |
6. A new insight into the species barrier of Mammalian Prions obtained from structure-based conservation analysis | |||
Yaofeng Wang,Bingjie Hu,Yuan Liu,Huili Zhang,Youtao Song | |||
Biology 17 December 2007 | |||
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Abstract:In the previous study done by Scott, M.R. et al, residues 184, 203 and 205 were predicted to contribute to an epitope modulating prion transmission, however, the evolutionary features of these residues were still unknown. To explore that, the functional domains (121-231) of 8 mammalian prions were examined by an informative and sensitive structural conservation analysis in this study. The results identified the three residues as highly structure-conserved but sequence-nonconserved. This suggested us the residues with these features may play an important role in modulating the transmission of prion diseases. Based on our hypothesis, residue 215 with the same structure feature was also predicted to involve in prion transmission. Subsequently, a phylogenetic tree was reconstructed. The topology of the tree can explain the mystery of species barrier occurred between the mammalian prions which cannot be deduced by simply sequence phylogenetic tree. | |||
TO cite this article:Yaofeng Wang,Bingjie Hu,Yuan Liu, et al. A new insight into the species barrier of Mammalian Prions obtained from structure-based conservation analysis[OL].[17 December 2007] http://en.paper.edu.cn/en_releasepaper/content/17036 |
7. Transgenic rice plants expressing rice dwarf virus outer coat protein gene (S8) are moderately resistant to virus infection | |||
Zheng Honghong,Wei Chunhong,Chen Shengxiang,Chen Zhangliang,Y. Li | |||
Biology 19 December 2005 | |||
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Abstract:We have previously obtained transgenic rice plants (Oryza sativa L.) containing S8 gene which encodes the outer coat protein of the rice dwarf virus (RDV) by particle bombardment. In this study, it was confirmed that the S8 gene has been stably inherited and expressed in the selfed T1 and T2 progenies of the primary trangenic rice plant line T0-2. Virus resistance assays revealed that both T1 and T2 transgenic plants exhibited resistance to RDV infection, indicating stable transmission of the trait of resistance. This study is the first report about the Pathogen-derived resistance in rice dwarf virus, a member of phytoreovirus. | |||
TO cite this article:Zheng Honghong,Wei Chunhong,Chen Shengxiang, et al. Transgenic rice plants expressing rice dwarf virus outer coat protein gene (S8) are moderately resistant to virus infection[OL].[19 December 2005] http://en.paper.edu.cn/en_releasepaper/content/4449 |
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