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1. Pathological lesion and the antigenic localization of highly pathogenic avian influenza virus in Sheldrake Ducks | |||
LIU Xiaojing,ZHENG Shimin,SUN Zhengyang,GAO Xueli,LIU Chaonan | |||
Animal Husbandry, Veterinary Medicine 14 May 2017 | |||
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Abstract:In this paper, to determine the pathogenicity and antigen distribution of China early H5N1 highly pathogenic avian influenza virus (HPAIV) A/GS/LN/45/04, the Sheldrake ducks were inoculated via the nostrils. Infected ducks appeared depression, orticollis and nystagmus clinical signs, hematoxylin and eosin (HE) stain found multiple organs showed different degrees of pathological changes. In addition, by Immuno-histochemistry (IHC) stain found that A/GS/LN/45/04 virus could duplicated in multiple organs, especially in brain and pancreas. These results could provide some important theoretical basis for the further study of Chinese AIVs. | |||
TO cite this article:LIU Xiaojing,ZHENG Shimin,SUN Zhengyang, et al. Pathological lesion and the antigenic localization of highly pathogenic avian influenza virus in Sheldrake Ducks[OL].[14 May 2017] http://en.paper.edu.cn/en_releasepaper/content/4733275 |
2. Effects of different forms and concentrations of selenium on ConA induced T-cell proliferation in primary cultured pig splenocytes | |||
Chen Xingxiang,Ren Fei,Gan Fang,Shi Xiuli,Li Junxian,Huang Kehe | |||
Animal Husbandry, Veterinary Medicine 24 November 2016 | |||
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Abstract:To better understand the role of selenium (Se) in immunity, a few studies have been explored in some immune cells in vitro, but the effects of different forms of selenium on primary cultured pig splenocytes remain largely unclear. In present study, primary cultured pig splenocytes were incubated with 0 (control) and vary concentrations of selenium (Se) as selenomethionine (SeMet), or sodium selenite (Na2SeO3) for 48 h in the presence of ConA. Compared to controls, significantly higher T-cell proliferation were observed in groups treated by 0.5-32 μM SeMet, while T-cell proliferation were significantly increased in Na2SeO3-treated splenocytes at 0.5-4 μM, with maximal effects at 2 μM, and inhibited in a dose-dependent manner at 8-32 μM Na2SeO3. Intracellular reduced glutathione (GSH) contents and GPx activity in all Se-treated splenocytes were significantly higher than those in controls. An increases of GPx1 mRNA level were obtained in all Se-treated splenocytes, but GPx4 mRNA level was not affected by Se treatment. And mercaptosuccinic acid, the inhibitor of the GPx1, significantly inhibited the proliferation improved by Se. We conclude that regulations of ConA-induced T-cell proliferation by different Se forms are different in primary cultured pig splenocytes, and GPx1 maybe the key enzyme. Our finding may contribute to understanding the differential influences of Se in different forms on various types of immune responses. | |||
TO cite this article:Chen Xingxiang,Ren Fei,Gan Fang, et al. Effects of different forms and concentrations of selenium on ConA induced T-cell proliferation in primary cultured pig splenocytes[OL].[24 November 2016] http://en.paper.edu.cn/en_releasepaper/content/4711505 |
3. Selenomethionine alleviates the AFB1-induced damages in primary cultured chicken hepatocytes by improving SelW expression to inhibit CYP450 1A5 expression | |||
Chen Xingxiang,Che Chaoping,Gan Fang,Pan Cuiling,Huang Kehe | |||
Animal Husbandry, Veterinary Medicine 24 November 2016 | |||
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Abstract:The aim of this study was to evaluate the effects of selenomethionine (SeMet) on the hepatotoxicity induced by Aflatoxin B1 (AFB1) in primary cultured chicken hepatocytes and explore its mechanism involved. The results revealed that the toxic effect of AFB1 on chicken hepatocytes is dose dependent and AFB1 at concentration of over 0.05 μg/mL caused significant increases in the levels of malondialdehyde and cytochrome P450 enzymes (CYP450 1A5) mRNA and reduction on the levels of intracellular glutathione (GSH) and total superoxide dismutase in hepatocytes when compared with the control (P < 0.05). Supplement with 2 μmol/L of SeMet exerted protective effect against the AFB1-induced damage in chicken hepatocytes by significantly increasing the mRNA level of Selenoprotein W (SelW) and decreasing the mRNA level of CYP450 1A5 (P < 0.05). Knockdown of SelW with SelW-specific siRNA significantly increased the mRNA and protein levels of CYP450 1A5 (P < 0.05) and further attenuated the protective effect of SeMet on the AFB1-induced damage. Thus, it can be concluded that selenomethionine alleviates the AFB1-induced damage in primary cultured chicken hepatocytes by improving SelW expression to inhibit CYP450 1A5 expression. | |||
TO cite this article:Chen Xingxiang,Che Chaoping,Gan Fang, et al. Selenomethionine alleviates the AFB1-induced damages in primary cultured chicken hepatocytes by improving SelW expression to inhibit CYP450 1A5 expression[OL].[24 November 2016] http://en.paper.edu.cn/en_releasepaper/content/4711502 |
4. Gambogic Acid Inhibit Hsp90 Expression and Restored growth plate in Thiram Induced Tibial Dyschondroplasia | |||
LI Jiakui,HUANG Shucheng | |||
Animal Husbandry, Veterinary Medicine 27 May 2016 | |||
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Abstract:Tibial Dyschondroplasia (TD) is an important leg problem in fast growing birds that disturbs the proximal growth plate; First time study was conduct to evaluate effect of Gambogic acid (GA) on Hsp90 expression and antioxidant capability in thiram induced Tibial dyschondroplasia. One hundred and fifty commercial chicks were distributed into three groups: Control (A), Thiram induced (B) and GA treated (C). Samples were collected at day 7 & 14 at end of experiment liver samples were collected to determine content of antioxidant enzymes. The results demonstrated that Hsp90 were highly expressed significantly (P>0.05) in thiram induced chicks that contributing to the development of TD and decrease in antioxidant capacity of liver. GA administration down regulated Hsp90 expressions levels and restore antioxidant capacity of liver significantly (P>0.05). In conclusion, GA return growth plate vascularization in TD affected chicks; it provides new clinical usage and protective role of this promising agent against TD. | |||
TO cite this article:LI Jiakui,HUANG Shucheng. Gambogic Acid Inhibit Hsp90 Expression and Restored growth plate in Thiram Induced Tibial Dyschondroplasia[OL].[27 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4694094 |
5. Orf in goats in China: prevalence and risk factors | |||
GAO Yang,ZHAO Yanqing,LIU Jianpeng,ZHOU Ming,LIU Heyuan,LIU Fang,YANG Wenyu,CHEN Dekun | |||
Animal Husbandry, Veterinary Medicine 24 May 2016 | |||
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Abstract:Orf is an important viral disease that affects goats and sheep and results in large economic losses. The aim of this study was to investigate the prevalence of orf and to identify the potential risk factors of this disease in the main breeding areas of China. Among 1241 blood samples collected from goats without clinical signs of orf, 433 samples (34.89%) were positive for orf virus infection, which was detected by polymerase chain reaction (PCR) targeting a partial B2L sequence of the viral genome. Moreover, a total of 874 buccal swab samples were collected, of which 64 samples (7.32%) were positive for the orf virus on the basis of PCR detection. According to logistic regression, all of the variables, including age, breed, location and farm management, had significant impacts on the prevalence of orf. Lambs under intensive management in Yunnan province were more susceptible to orf virus infection than animals in other groups. Anglo-Nubian goats were at more risk of orf positivity than other breeds, whereas Saanen dairy goats were at significantly less risk. In summary, as the first epidemiological study of orf in China, our investigation suggested that orf is a neglected disease that requires more attention in the future.????? | |||
TO cite this article:GAO Yang,ZHAO Yanqing,LIU Jianpeng, et al. Orf in goats in China: prevalence and risk factors[OL].[24 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4691826 |
6. Isolation, Molecules characterization and Immunostimulatory activity of polysaccharide from Yu-Ping-Feng | |||
ZHAO Xiaona | |||
Animal Husbandry, Veterinary Medicine 05 May 2016 | |||
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Abstract:Yu-Ping-Feng is a well-known traditional Chinese multi herbal formula, and it is frequently being used to treat cold and flu and inflammation-associated diseases. We aim to evaluate the immunostimulatory activity of polysaccharide from Yu-Ping-Feng(YPF-PS) in vitro on chicken macrophage to make clear the bioactive molecular of Yu-Ping-Feng. Gel permeation chromatography (GPC) and gas chromatography?mass spectrometry (GC-MS) analysis, amino acid analysis and nuclear magnetic resonance (NRM) spectroscopy elucidated the structure of the YPF-PS. In order to evaluate the biological activities of YPF-PS, lipopolysaccharides (LPS)-induced cell proliferation and phagocytosis rate and the levels of cytokine: nitric oxide (NO), tumor necrosis factor alpha (TNF-α), interleukin-1 beta, interleukin-6 (IL-1β, IL-6), interferon beta (IFN-β) and costimulatory molecules CD80, CD86 in chicken macrophage were detected. The results indicated that molecular weight the weight of YPF-PS was distributed on 5 KD~500 KDa, which is a mixture of glucose (Glu), fructose (Fru), arabinose (Ara) and few rhamnose with the molar ratio of 61.29: 55.47: 99.67: 19.58, having anomeric carbon signals of β-L-arabino furanoside, α-L-rhamnoside, α-D-glucopyranoside and β-D-fructofuranose. Furthermore, cell immunology experiments show that YPF-PS at dosage of 15.62 μgmL -1 could increase the LPS-induced cell proliferation and phagocytosis rate significantly in chicken macrophage, moreover, the levels of cytokine: NO, TNF-α, IL-1β, IL-6 and IFN-βin cells elevated obviously, equally shocking, costimulatory molecules CD80, CD86 were detected having a tremendous enhancement. We believe that the YPF-PS has potent immunomodulatory properties and could be explored as a novel potential immunostimulant for the food and pharmaceutical purpose. | |||
TO cite this article:ZHAO Xiaona. Isolation, Molecules characterization and Immunostimulatory activity of polysaccharide from Yu-Ping-Feng[OL].[ 5 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4686482 |
7. Effects of polysaccharides from Platycodon Grandiflorum on immunity-enhancing activity in vitro | |||
ZHAO Xiaona | |||
Animal Husbandry, Veterinary Medicine 05 May 2016 | |||
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Abstract:This study evaluated the immunity-enhancing activity of polysaccharides from Platycodon Grandiflorum (PGPS) in vitro. Lymphocyte proliferation, cell cycle distribution, and percentages of CD4+ and CD8+ T cells were investigated. Different concentrations of PGPS (PGPStc, PGPS60c, PGPS80c and PGPStp) were added to peripheral blood T lymphocytes. Results showed that PGPStc markedly enhanced T lymphocyte proliferation individually or synergistically with phytohemagglutinin (PHA) at most concentrations and the lymphocyte proliferation rates were the highest. Then, the active sites of PGPStc and PGPS60c were subsequently selected. Lymphocyte cell cycle distribution and percentages of CD4+ and CD8+ T cells were determined by flow cytometry. At most time points, PGPStc could promote lymphocytes enter into S and G2/M phases, while increasing the percentages of CD4+ and CD8+ T cells. These results indicate that PGPStc enhances the immune functions, suggesting that PGPStc could be a potential immunopotentiator for further in vivo and clinical trial experiments. | |||
TO cite this article:ZHAO Xiaona. Effects of polysaccharides from Platycodon Grandiflorum on immunity-enhancing activity in vitro[OL].[ 5 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4686485 |
8. Outbreaks of Hemangiomas Associated with Avian Leukosis Virus Subgroup J infection in Commercial Layer Flocks in China | |||
WANG Guihua,YU Linlin,QIU Bo,CHENG Ziqiang | |||
Animal Husbandry, Veterinary Medicine 05 December 2015 | |||
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Abstract:Outbreaks of skin and internal neoplasia in commercial Hyline layer flocks occurred in eastern China during the years of 2009 to 2010. Anemia, reduction in egg production and high mortality (up to 20%) were common characters of affected flocks. Most of tumors were present in the skin of trunk, digitus, joint, face and wing, and in the liver, spleen, heart, small intestine and pancreas at 26-week of age. The tumors looked exactly like blood blisters in various sizes. Microscopically, they were defined as hemangiomas of typical capillary and cavernous form, characterized by greatly distended blood spaces with thin walls composed of endothelial cells. Myelocytomas, fibrosarcomas and myxoma were observed in few hemangiomas cases. Three strains of avian leukosis virus subgroup J (ALV-J) were isolated from DF-1 culture (WS0703, WS0704 and WS0705). Sequences analysis of the gp85 gene of the three strains revealed that these viruses are closely related to HPRS-103 (98.3%), a prototype of ALV-J. There was no evidence of Marek's disease virus (MDV) infection. Proviral DNA of avian leukosis virus subgroup A/B (ALV-A/B) and Reticuloendotheliosis virus (REV) was not detected by polymerase chain reaction (PCR) either in tumors or in DF-1 cultures. The report demonstrated that hamangiomas presentation associated with not only multipotential oncogenicity of ALV-J but also the line of chicken. The pathogenesis need to be further investigated.????? | |||
TO cite this article:WANG Guihua,YU Linlin,QIU Bo, et al. Outbreaks of Hemangiomas Associated with Avian Leukosis Virus Subgroup J infection in Commercial Layer Flocks in China[OL].[ 5 December 2015] http://en.paper.edu.cn/en_releasepaper/content/4669255 |
9. Sterculic acid and its analogues are potent inhibitors of Toxoplasma gondii | |||
HAO Pan,ALARAJ Intisar Q. M.,AL DULAYYMI Juma’a R.,BAIRD S. Mark,LIU Jing,LIU Qun | |||
Animal Husbandry, Veterinary Medicine 02 December 2015 | |||
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Abstract:Toxoplasmosis is a serious disease caused by Toxoplasma gondii, one of the most widespread parasites in the world. Lipid metabolism is important in the intracellular stage of Toxoplasma gondii. Stearoyl-CoA desaturase (SCD), a key enzyme for the synthesis of unsaturated fatty acid, was predicted to exist in T. gondii. Sterculic acid has been shown to specifically inhibit SCD activity. Here, we examined whether sterculic acid and its methyl ester analogues exhibit anti-T. gondii effect in vitro. T. gondii- infected Vero cells disintegrated at 36h because of the propagation and egress of intracellular tachyzoites. All test compounds inhibited tachyzoite propagation and egress, reducing the number of ruptured Vero cells by the parasites. Sterculic acid and its methyl esters also inhibited replication of intracellular tachyzoites in HFF cells. Among the test compounds, sterculic acid showed the most potent activity against T gondii, with an EC50 value of 36.2 μM, compared with EC50 values of 248-428 uM for the methyl esters. Our study demonstrated that sterculic acid and its analogues are effective in the inhibition of T. gondii growth in vitro, suggesting these compounds or analogues targeting SCD could be effective agents for the treatment of toxoplasmosis. | |||
TO cite this article:HAO Pan,ALARAJ Intisar Q. M.,AL DULAYYMI Juma’a R., et al. Sterculic acid and its analogues are potent inhibitors of Toxoplasma gondii[OL].[ 2 December 2015] http://en.paper.edu.cn/en_releasepaper/content/4667291 |
10. SEROPREVALENCE OF ANTIBODIES AGAINST NEOSPORA CANINUM IN PèRE DAVID'S DEER (ELAPHURUS DAVIDIANUS) IN BEIJING | |||
HAN Fangjie,FU Yong,ZHANG Changsheng,LIU Qun,LIU Jing | |||
Animal Husbandry, Veterinary Medicine 30 June 2015 | |||
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Abstract:Neospora caninum is an apicomplexan protozoan that causes neuromuscular disease in canids and abortion in cattle worldwide. In China, sera from 49 Père David's deer (Elaphurus davidianus) were investigated for the presence of antibodies against N. caninum using indirect fluorescence antibody tests (IFAT) and western blots. N. caninum antibodies were found in 26.53% (13 positive/49 tested) of the Père David's deer according IFAT and the western blots. Western blot analysis revealed there was seroreactivity against immunodominant N. caninum antigens, and the bands were 16, 25, and 37 kDa in size together with other visible bands. This is the first study of antibodies against N. caninum in Père David's deer from China. | |||
TO cite this article:HAN Fangjie,FU Yong,ZHANG Changsheng, et al. SEROPREVALENCE OF ANTIBODIES AGAINST NEOSPORA CANINUM IN PèRE DAVID'S DEER (ELAPHURUS DAVIDIANUS) IN BEIJING[OL].[30 June 2015] http://en.paper.edu.cn/en_releasepaper/content/4649079 |
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