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	1. Correlation between SmCPS1 promoter polymorphism and tanshinones contents in Salvia miltiorrhiza
	SUN Ying,CHEN Xin,GAN Xiaoyan,YAN Zhuyun,MU Dixiu,WANG Qingrong
	Traditional Chinese Medicine and Pharmacology 05 September 2017
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	Abstract:To investigate the relationship between SmCPS1 promoter polymorphism and the contents of tanshinones. We sequenced the SmCPS1 gene promoter region together with 388bp downstream from the translation start site and the content of tanshinones of 12 different germplasm of Salvia miltiorrhiza. To separate the functional polymorphic loci that may be related with the content of tanshinones from those not, the cis-elements of SmCPS1 promoter were predicted and analyzed by the Plant Transcriptional Regulatory Map database. We found 1) there was a different correlation between the polymorphism in the promoter region and the contents of the tanshinones. 2) Polymorphism on nine cis-elements, an 33bp insertion/deletion sequence and three tandem repeat variations in the sequence of SmCPS1 promoter may be related to the contents of tanshinones. These findings showed the relationships between SmCPS1 promoter polymorphism and tanshinones.
	TO cite this article:SUN Ying,CHEN Xin,GAN Xiaoyan, et al. Correlation between SmCPS1 promoter polymorphism and tanshinones contents in Salvia miltiorrhiza[OL].[ 5 September 2017] http://en.paper.edu.cn/en_releasepaper/content/4741360


	2. Factors Affecting Embryogenic Callus Production and Plant Regeneration from Anther Culture of Bupleurum chinense
	YANG Chengmin,ZHAO Yukai,WEI Jianhe,ZHAO Lizi,SUI Chun,Zhang Zhen,CUI Luying
	Traditional Chinese Medicine and Pharmacology 28 January 2011
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	Abstract:To evaluate the influences of the genotypes, anther developmental stages and cultural conditions on the efficiency of embryogenic callus induction and plant regeneration in the anthers culture of Bupleurum chinense. Methods The different effects such as four genotypes, plant growth regulators, and temperature condition were compared in the experiments. Results The most embryogenic calli was achieved for the genotypes 'ZCYH', 'Z4' and 'Z5' at the early- to middle-uninucleate stages, for genotype 'ZPM1' at the tetrad stage. Cold pretreatment increased the production of the embryogenic callus, and 4-d cold pretreatment improved the production of embryogenic callus from 0 to 2.2% and 5.0% for genotypes 'ZPM1' and 'ZCYH', respectively. No embryogenic callus were induced in the medium containing less than 0.75 mg/L 2,4-D. The highest regeneration rate (34.6%) was obtained in the half-strength MS salts regeneration medium supplemented with BA 0.1 mg/L. The low concentration BA was able to promote the embryogenic callus formation and subsequent plantlet regeneration via somatic embryogenesis. Chromosome counting of regenerated plantlets showed mostly diploid plant (2n=12) with only one haploid plant (n=6). Because of the low rate of microspore embryo formation, we only track the process of embryogenesis from the connective tissue, instead of microspore by histological observations. Conclusion This study developed an efficient system embryogenic for callus induction and plant regeneration system from anthers of B. chinense. This is the first report on the haploid plantlet through the anther culture of B. chinense.
	TO cite this article:YANG Chengmin,ZHAO Yukai,WEI Jianhe, et al. Factors Affecting Embryogenic Callus Production and Plant Regeneration from Anther Culture of Bupleurum chinense[OL].[28 January 2011] http://en.paper.edu.cn/en_releasepaper/content/4409604

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