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1. NGF contribute to acid-induced articular chondrocytes apoptosis by modulating ASIC1a/NLRP1/Caspase-1 signaling axis | |||
HU Wei | |||
Pharmacy 14 May 2018 | |||
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Abstract:Objective: Nerve growth factor (NGF) is key regulators in the pathogenesis of the rheumatoid arthritis (RA) diseases. However, the potential role of NGF in RA remains unclear. It has been shown that ASIC1a is an extracellular pH sensor in articular chondrocytes and RA. Moreover, NGF modulates the expression of ASICs in sensory neurons sensitization. In this study, we examined whether NGF contribute to ASIC1a expression could affect acid-induced apoptotic injury to articular chondrocytes. Methods: The primary rat articular chondrocytes were isolated from Sprague-Dawley rats. Apoptosis of chondrocytes was observed by the terminal deoxyribonucleotidyl transferase- mediated dUTP nick-end labeling method as well as propidium iodide labeling methods. Treatment of articular chondrocytes with NGF, ASIC1a-siRNA and acid, the expression levels of NGF, ASIC1a, NLRP1 and Caspase-1 were examined by qRT-PCR and western blotting, respectively. Results: We found that up-regulation of ASIC1a in acid-induced articular chondrocytes is associated with NGF treatment. Knockdown of ASIC1a inhibited NLRP-1 expression in acid-induced articular chondrocytes. Knockdown of ASIC1a suppressed acid-induced articular chondrocytes apoptosis. Moreover, we investigated the effect of ASIC1a on NLRP1/Caspase-1 pathway. Our results demonstrated that NGF contribute to acid-induced articular chondrocytes apoptosis by modulating ASIC1a/NLRP1/Caspase-1 signaling axis. Conclusion: Taken together, these results indicated that NGF promotes acid-induced articular chondrocytes apoptosis by up-regulation of ASIC1a/NLRP1/Caspase-1 signaling axis. | |||
TO cite this article:HU Wei. NGF contribute to acid-induced articular chondrocytes apoptosis by modulating ASIC1a/NLRP1/Caspase-1 signaling axis[OL].[14 May 2018] http://en.paper.edu.cn/en_releasepaper/content/4745087 |
2. Acid-sensing ion channel 1a regulates matrix metabolism of rat | |||
weihu,Cheng Sun,shiming wang | |||
Pharmacy 25 April 2017 | |||
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Abstract:Objectives: ASIC1a has a wide range of biological functions, plays an important role in cell metabolic activity, Our research has shown that extracellular pH plays an important role in matrix synthesis and metabolism in articular chondrocytes by activated ASIC1a. However, little is kown about the underlying mechanism of ASIC1a on matrix synthesis and metabolism in articular chondrocytes. The present study was undertaken to investigate the mechanism of ASIC1a on matrix synthesis and metabolism in articular chondrocytes by acidosis. Down-regulation of GAG, HYP, MMP-2, MMP-9, TIMP-1 and TIMP-2 mRNA expression and production was abolished by inhibit acidosis- elicited increase in intracellular Ca2+ concentration. Pretreatment with BAPTA-AM (Ca2+ chelator) abolished acidosis induced ERK1/2, p38 MAPK, c-jun and c-fos activation. PD98059 (ERK1/2 inhibitor) attenuate c-jun activation and restored GAG, HYP, TIMP-1 and TIMP-2 mRNA expression and production. SB203580 (p38 MAPK inhibitor) inhibit c-fos activation and restored MMP-2 and MMP-9 mRNA expression and production. We founded that bolcking ASIC1a inhibit the effect of extracellular acidosis on GAG, HYP, MMP-2, MMP-9, TIMP-1 and TIMP-2 mRNA expression and production in articular chondrocytes. Taken together, our data indicate that blocking acid-sensing ion channel 1a inhibit abberant matrix synthesis and metabolism from acid-induced articular chondrocytes via Ca2+-mediated activation of p38 MAPK/c-jun and ERK/c-fos pathway. | |||
TO cite this article:weihu,Cheng Sun,shiming wang. Acid-sensing ion channel 1a regulates matrix metabolism of rat[OL].[25 April 2017] http://en.paper.edu.cn/en_releasepaper/content/4727034 |
3. Fluorescent carbon dots derived from lactose for assaying folic acid | |||
CHEN Zhangbao,WANG Jing,MIAO Hong,WANG Lan,YANG Xiaoming | |||
Pharmacy 01 December 2015 | |||
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Abstract:The fluorescent carbon dots were successfully synthesized by simply heating the mixture of lactose and NaOH solution. The as-synthesized carbon dots had been systematically characterized by fluorescence, FTIR, HR-TEM and 13C-NMR. Since the fluorescence of the carbon dots were efficiently quenched by folic acid, the carbon dots were employed as selective fluorescence probes for detecting of folic acid, depending on the formation of hydrogen bond among the functional group of folic acid (-OH, -COOH and -NH2) and -OH and -COOH of the carbon dots. Moreover, the decrease of fluorescence intensity was capable of detecting folic acid in a linear range of 6×10-5 mol/L-8×10-8mol/L with a detection limit of 1.2×10-9 mol/L at a signal-to-noise ratio of 3, suggesting a promising assay for folic acid. Significantly, the practicability of this fluorescence probe to assay folic acid in human urine samples was further evaluated. | |||
TO cite this article:CHEN Zhangbao,WANG Jing,MIAO Hong, et al. Fluorescent carbon dots derived from lactose for assaying folic acid[OL].[ 1 December 2015] http://en.paper.edu.cn/en_releasepaper/content/4667768 |
4. Endoplasmic Reticulum Stress-induced hepatic stellate cell apoptosis through calcium-mediated JNK/P38 MAPK and Calpain/Caspase-12 pathways | |||
LI Xiaohui,WANG Yarui,WANG Huan,HUANG Cheng,HUANG Yan,LI Jun | |||
Pharmacy 11 March 2014 | |||
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Abstract:Recent reports considered that it was the disturbance of calcium homeostasis and the accumulation of misfolded proteins in the endoplasmic reticulum (ER) that activated hepatic stellate cells (HSCs) apoptosis and promoted fibrosis resolution. However, the signal-transducing events that are activated by ER stress after HSCs activation were incompletely understood. In this study, we induced ER stress with thapsigargin (TG), and determined the activation of calpain and the cleavage of caspase by analyzing the protein levels and the correspondingly increased intracellular calcium levels and the induction of the proapoptotic transcription factor CHOP. Moreover, the phosphorylation of JNK/p38 MAPK was followed by the activation of the executioner caspases, caspase-3. As expected, preventing an increase in intracellular calcium levels using intracellular calcium chelators, EGTA and BAPTA/AM, could substantially inhibit the phosphorylation of JNK/p38 MAPK, abolish the activation of calpains, namely caspase-12, caspase-9 and caspase-3, and provide significant protection for TG-treated activated HSCs. Interestingly, pretreatment with p38 MAPK inhibitor SB202190, JNK inhibitor SP600125, the pan-caspase inhibitor z-VAD-FMK, or calpain inhibitors calpeptin, significantly reduced the cell apoptosis and the cleavage of caspase-12 and caspase-3. However, pretreatment with z-VAD-FMK failed to reduce the activation of calpain. Additionally, pretreatment with SB202190 and SP600125 also decreased the expression of CHOP. Importantly, PDGF-induced collagen Col1α1 and α-smooth muscle actin (α-SMA), markers for the perpetuation phase of HSCs activation, were inhibited in TG-treated activated HSCs. These findings showed that the calpain/caspase activation induced by ER stress and the JNK/p38 MAPK phosphorylation induced by the increase of intracellular calcium concentration releasing from ER are the novel signaling pathway underlying the molecular mechanism of fibrosis recovery. | |||
TO cite this article:LI Xiaohui,WANG Yarui,WANG Huan, et al. Endoplasmic Reticulum Stress-induced hepatic stellate cell apoptosis through calcium-mediated JNK/P38 MAPK and Calpain/Caspase-12 pathways[OL].[11 March 2014] http://en.paper.edu.cn/en_releasepaper/content/4589681 |
5. A simple HPLC-UV method for the determination of a novel anticancer candidate compound Z-Gly-Pro-doxorubicin in rat bile and its application to biliary excretion study | |||
HUANG Weixin,WANG Jingjing,MA Li,HAN Hai,XU Jun,CAI Shaohui | |||
Pharmacy 09 July 2013 | |||
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Abstract:Z-Gly-Pro-doxorubicin (Z-GP-DOX), a prodrug of doxorubicin (DOX), has been proved in our previous study to be a good prodrug to achieve targeted delivery of DOX. Also, the pharmacokinetic study of Z-GP-DOX in rat plasma has been completed. In this paper, a simple, sensitive and specific HPLC-UV method for the determination of Z-GP-DOX in rat bile was established and validated. Following liquid-liquid extraction, chromatographic separation was accomplished by the mobile phase acetonitrile-0.1%trifluoroacetic acid (50:50, v/v) with a C18 chromatography column at a flow rate of 1 mL/min, room temperature and detection wavelength of 495 nm. The retention time of Z-GP-DOX was 6.6 min. A linear curve over the concentration range of 1-1200 μg/mL (r2 > 0.999) was established, and the LOD and LOQ for Z-GP-DOX were 0.5 and 1 μg/mL, respectively. Good precision and accuracy at concentrations of 2, 600 and 1000 μg/mL were obtained. The mean extraction recovery of Z-GP-DOX in bile was over 82.92% at the studied concentrations. The intra-day and inter-day relative standard deviations were generally less than 10%. This method was successfully applied to biliary excretion study in rats after intravenous administration of Z-GP-DOX. Bile samples were collected from bile duct cannulated rats after an intravenous bolus dose of 10 mg/kg or 20 mg/kg Z-GP-DOX, and the concentrations were measured by HPLC-UV. The results showed that the concentration of Z-GP-DOX in rat bile was much higher than that in plasma. After dosing, 28.14 ± 2.13% and 20.25 ± 3.59% of the dose were excreted into bile in unchanged form after a 12-h collection. The present study will contribute to supplementing the previous pharmacokinetic study of Z-GP-DOX in rats and will be helpful to improve the druggability study of Z-GP-DOX. | |||
TO cite this article:HUANG Weixin,WANG Jingjing,MA Li, et al. A simple HPLC-UV method for the determination of a novel anticancer candidate compound Z-Gly-Pro-doxorubicin in rat bile and its application to biliary excretion study[OL].[ 9 July 2013] http://en.paper.edu.cn/en_releasepaper/content/4551463 |
6. Comparative studies of paeoniflorin and albiflorin from Chinese Paeony on Anti-inflammatory activities | |||
GAO Teng,WANG Qiangsong,CUI Yuanlu | |||
Pharmacy 20 May 2013 | |||
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Abstract:Purpose: Chinese Paeony has been used for more than 1000 years in traditional Chinese medicine for the treatment of gynecological problems, cramp, pain, giddiness, and congestion. Paeoniflorin and albiflorin, monoterpene glycosides isolated from Chinese Paeony, possess a variety of pharmacological activities. The present study was investigated the anti-inflammatory activities of paeoniflorin and albiflorin using models of Lipopolysaccharides (LPS) induced RAW 264.7 cells. Methods: Production of nitric oxide (NO) was measured by the Griess colorimetric method and (ELISA). In addition, interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) synthesis were analyzed using enzyme-linked immunosorbent assay (ELISA). The protein expression of cyclooxygenase-2 (COX-2) was detected by cell-based ELISA. The gene expression levels of inducible nitric oxide synthase (iNOS), COX-2, TNF-α, IL-6 were detected by quantitative real-time reverse-transcription polymerase chain reaction (real-time RT-PCR). Results: The results showed that paeoniflorin and albiflorin could inhibit LPS-induced NO, TNF-α, IL-6 production and protein expression of COX-2. Furthermore, they could inhibit the mRNA expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), TNF-α, IL-6. Conclusion: These results show that albiflorin has the similar pharmacological effects to paeoniflorin on anti-inflammatory activities, which will provide a new evidence to pay albiflorin more attention to main quality control indicators of Paeoniae Radix and edit the new Chinese Pharmacopoeia. | |||
TO cite this article:GAO Teng,WANG Qiangsong,CUI Yuanlu. Comparative studies of paeoniflorin and albiflorin from Chinese Paeony on Anti-inflammatory activities[OL].[20 May 2013] http://en.paper.edu.cn/en_releasepaper/content/4544036 |
7. CYP3A4 and CYP3A5 Polymorphism Effects on Tacrolimus Pharmacokinetics in Chinese Adult Renal Transplant Recipients: A Population Pharmacokinetic Analysis | |||
ZUO Xiaocong,Chee M. Ng,Jeffrey S.Barrett,LUO Aijing,ZHANG Bikui,DENG Chenhui,XI Lanyan,CHENG Ke,MING Yingzi,YANG Guoping,PEI Qi,ZHU Lijun,YUAN Hong,LIAO Haiqiang,DING Junjie,WU Di,ZHOU Yanan,JING Ningning,HUANG Zhijun | |||
Pharmacy 25 April 2013 | |||
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Abstract:Objective Tacrolimus is used clinically for long-term treatment of antirejection of transplanted organs in liver and kidney transplant recipients though dose optimization is poorly managed. The goal of this study was to investigate the association between tacrolimus pharmacokinetic variability and CYP3A4 and CYP3A5 genotype by a population pharmacokinetic analysis based on routine drug monitoring data in adult renal transplantation recipients. Methods Trough tacrolimus concentrations were obtained from 161 adult kidney transplant recipients post-transplantation. The population pharmacokinetic analysis was performed using the nonlinear mixed-effect modeling software NONMEM version 7.2. The CYP3A4*1G and CYP3A5*3 genetic polymorphisms from studied patients were determined by direct sequencing using a validated automated genetic analyzer. Results A one-compartment model with first-order absorption and elimination adequately described the pharmacokinetics of tacrolimus. Covariates including CYP3A5*3 and CYP3A4*1G alleles and hematocrit were retained in the final model. The apparent clearance of tacrolimus was about 2-fold higher in kidney transplant patients with CYP3A5*3 and CYP3A4*1G higher enzymatic activity (with the CYP3A5 *1/*1 or *1/*3 and CYP3A4*1/*1G or CYP3A4*1G/*1G) compared to those with lower enzymatic activity (CYP3A5 *3/*3 and CYP3A4*1/*1). Conclusion This is the first study to extensively investigate the effect of CYP3A4*1G and CYP3A5*3 genetic polymorphisms and hematocrit value on tacrolimus pharmacokinetics in renal transplantation recipients. The findings suggest that CYP3A5*3 and CYP3A4*1G polymorphisms and hematocrit are determinant factor in the apparent clearance of tacrolimus. The initial dose design is mainly based on CYP3A5 and CYP3A4 genotypes as well as hematocrit.This results may also be good for the maintenance tacrolimus dose optimization and help to avoid fluctuating tacrolimus levels and improve the efficacy and tolerability of tacrolimus in kidney transplant recipients. | |||
TO cite this article:ZUO Xiaocong,Chee M. Ng,Jeffrey S.Barrett, et al. CYP3A4 and CYP3A5 Polymorphism Effects on Tacrolimus Pharmacokinetics in Chinese Adult Renal Transplant Recipients: A Population Pharmacokinetic Analysis[OL].[25 April 2013] http://en.paper.edu.cn/en_releasepaper/content/4530503 |
8. Determination of 5-Hydroxyindole-3-acetic acid, dihydroxyphenylacetic acid and homovanillic acid in the brains of freely moving rats using microdialysis coupled with HPLC-ECD | |||
LIU Rui,DUAN Jin-ao,GUO Jianming,TANG Yuping,QIAN Dawei | |||
Pharmacy 12 January 2013 | |||
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Abstract:In present paper, microdialysis (MD) combined with high performance liquid chromatography with electrochemical detection (HPLC-ECD) was applied for the determination of 5-hydroxyindole-3-acetic acid (5-HIAA), dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in the hypothalamus of LPS-induced hyperthermia rats. The extracellular neurotransmitters of 5-HIAA, DOPAC and HVA could be detected increasing significantly in rat brain 150 min after LPS injection. The result showed that dynamic change of neurotransmitters in rat brain could be determined easily by MD coupled with HPLC-ECD. Furthermore, the advantage that fewer animals sacrifice occurred in the microdialysis experiment was confirmed. | |||
TO cite this article:LIU Rui,DUAN Jin-ao,GUO Jianming, et al. Determination of 5-Hydroxyindole-3-acetic acid, dihydroxyphenylacetic acid and homovanillic acid in the brains of freely moving rats using microdialysis coupled with HPLC-ECD[OL].[12 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4511624 |
9. Molecular Docking Identifies the Binding Sites of the Ligands Specifically to TCPTP | |||
Sun Suxia,Liang Jing,Wang Runling,Wang Shuqing,Cheng Xianchao,Dong Weili | |||
Pharmacy 17 April 2012 | |||
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Abstract:We have studied on T-cell protein-tyrosine phosphatase (TCPTP) as a model phosphatase in an attempt to seek the most favorable binding sites of TCPTP interacting with ligands and find out differences in substrate recognition by protein tyrosine phosphatase 1B (PTP1B) and TCPTP. The xalylarylaminobenzoic acids derivatives and 1,2,3,4-Tetrahydroisoquino- linyl (TIQ) sulfamic acid derivative were selected in this study. To better understand the structural and chemical features responsible for the recognition mechanism, the Autodock 4.0 was performed as automated molecular docking program to explore the binding pocket sites of this enzyme. Two key sites (Ⅰand Ⅲ) were found of the TCPTP contributing towards the binding of these compounds. SiteⅠ residues involved in forming two important hydrogen bonds from the enzyme were: Gln260 and Arg222. We also found that site Ⅲ consists of two main residues: Tyr22 and Pro262, which involved in hydrophobic interactions with the ligands. And the results of molecular docking showed that residue Asp48 played an important role in substrate binding. The interaction model of TCPTP inhibitors was similar and the difference in biologic activities of these inhibitors can be well explained. This study will help in the rational design of potent and selective PTP1B inhibitors over TCPTP. | |||
TO cite this article:Sun Suxia,Liang Jing,Wang Runling, et al. Molecular Docking Identifies the Binding Sites of the Ligands Specifically to TCPTP[OL].[17 April 2012] http://en.paper.edu.cn/en_releasepaper/content/4475056 |
10. Modulation of adult hippocampal neurognensis by telomerase: implication in depressive disorder | |||
ZHOU Qigang,HU Yao,LIU Mengying,JIN Xing,SUN Weixiang,ZHU Dongya | |||
Pharmacy 17 February 2012 | |||
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Abstract:Adult hippocampal neurogenesis is modulated by stress and various antidepressants. Neuronal stem cells express high levels of telomerase in adult hippocampus. Here, we used 3'-azido-deoxythymidine (AZT), a nucleoside reverse transcriptase inhibitor, and constructed recombinant adenovirus vector expressing mouse telomerase reverse transcriptase (Ad-mTERT-GFP) to investigate whether hippocampal telomerase implicate in pathophysiology of depression by regulating hippocampal neurogenesis. We found that infusion of AZT into hippocampal DG resulted in a decrease of neurogenesis in the hippocampus without causing neuronal degeneration, whereas infusion of Ad-mTERT-GFP into hippocampal DG upregulated hippocampal neurogenesis. The mice infused with AZT exhibited depression-like behaviors whereas the mice infused with Ad-mTERT-GFP exhibited antidepression-like behaviors both about 4 weeks after infusion. In addition, chronic stress resulted in a decrease in mTERT content in the hippocampus, reversed by chronic fluoxetine treatment. Furthermore, mTERT content in the SVZ, another telomerase expressed place in the adult brain, was unaffected after chronic stress exposure and infusion of AZT into SVZ did not induce depression-like phenotype. These results suggest that hippocampal telomerase is involved in the modulation of depression-related behaviors, possibly by regulating adult hippocampal neurogenesis. | |||
TO cite this article:ZHOU Qigang,HU Yao,LIU Mengying, et al. Modulation of adult hippocampal neurognensis by telomerase: implication in depressive disorder[OL].[17 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4466920 |
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