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1. Isolate, puri?cation and antioxidant activities of polysaccharides from Dioscoreae bulbiferae | |||
CUI Hongxia,SU Yan | |||
Biology 29 December 2017 | |||
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Abstract:A water-soluble polysaccharide from Dioscorea bulbifera (Dioscoreaceae) rhizome was isolated by extraction with water extract and alcohol precipitate and further purified by DEAE cellulose-52 chromatography to yield DBP-1 and DBP-2. The total yield rate of the polysaccharides was 4.3 %. Molecular weights were determined by high performance gel permeation chromatography (HPGPC). The molecular weights of DBP-1 and DBP-2 were 122435Da and 5180Da, respectively. The antioxidant activity of DBP-1 and DBP-2 were evaluated with the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals in vitro, and the results indicated that DBP-1 and DBP-2 had good antioxidant activity. These results suggested that the polysaccharides (DBP-1 and DBP-2) obtained from the Dioscorea bulbifera might be suitable for use as functional foods and potential therapeutic agents for human aging. | |||
TO cite this article:CUI Hongxia,SU Yan. Isolate, puri?cation and antioxidant activities of polysaccharides from Dioscoreae bulbiferae[OL].[29 December 2017] http://en.paper.edu.cn/en_releasepaper/content/4743023 |
2. TEMPRANILLO1 transcription factor AtTEM1 negatively regulates drought and mannitol tolerance in Arabidopsis | |||
LUO Guangyu,LIU Ailing,ZHOU Xiaoyun,ZHANG Xianwen,CHEN Xinbo | |||
Biology 11 May 2016 | |||
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Abstract:Arabidopsis TEMPRANILLO 1 (AtTEM1) is a member of the RAV transcription factor subfamily with plant-specific AP2 and B3 domains. Which are known to negatively control plant growth and delay flowering time. In this study, we have demonstrated that Arabidopsis AtTEM1 is localized in the nucleus, consistent with its putative role as a transcription factor. Expression of AtTEM1 was strongly induced by drought and mannitol treatments. Drought tolerances were significantly decreased in 35S::AtTEM1 transgenic Arabidopsis, but were enhanced in the AtTEM1 mutant plants. Under drought and mannitol stress, the 35S::AtTEM1 transgenic Arabidopsis exhibited increased relative electrical conductivity (REC) and content of malondialdehyde (MDA) and decreased proline content compared with the wild type, while the lower REC and MDA content and increased proline content were found in the AtTEM1 mutant plants. These results suggest that AtTEM1 functions as a negative regulator in response to drought and mannitol stresses in Arabidopsis. AtTEM1 also acts as a negative regulator for flowering time response. The 35S::AtTEM1 plants bolted later than the wildtype and produced extra rosette leaves before flowering, however the AtTEM1 mutant plants bolted earlier but produced almost the same rosette leaves before flowering. | |||
TO cite this article:LUO Guangyu,LIU Ailing,ZHOU Xiaoyun, et al. TEMPRANILLO1 transcription factor AtTEM1 negatively regulates drought and mannitol tolerance in Arabidopsis[OL].[11 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4687336 |
3. Study on Endophytic fungi producing Orange Pigment isolated from Ginkgo Biloba L. | |||
Jia-Jia Liu,Shu-Juan Chen,Han-Xiang Gong | |||
Biology 07 August 2008 | |||
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Abstract:More than seventy endophytic fungi were isolated by aseptic techniques from the phloem of the root of Ginkgo biloba L.. They were cultured in the potato dextrose (PD) liquid medium and one endophytic fungus (Gh01) was proved can produce orange pigment. The orange pigment was identified as quercetin glycoside by chemical reaction and HPLC. This is the first report on quercetin glycoside produced by endophytic fungus. The effects of the carbon and nitrogen sources, metal ions, initial pH and incubation temperature on pigment production of the endophytic fungus were evaluated. The optimal temperature and initial pH for pigment production in PD liquid medium were 28℃ and 7.0, respectively. Through the orthogonal trial, 20 g/L Glucose and 5 g/L peptone were the most suitable carbon and nitrogen source, 1 g/L chloridize zinc could increase the yield of pigment. Under the optimal conditions established, the maximum yield of the pigment was 27.515 g/L after 120 hours’ successive culture. | |||
TO cite this article:Jia-Jia Liu,Shu-Juan Chen,Han-Xiang Gong. Study on Endophytic fungi producing Orange Pigment isolated from Ginkgo Biloba L.[OL].[ 7 August 2008] http://en.paper.edu.cn/en_releasepaper/content/23292 |
4. Effect of silver nitrate on callus regeneration and activity of some enzymes during tissue culture of wheat immature inflorescence | |||
Xiangru Liao,Lei Tang,Feng Zhang | |||
Biology 01 November 2005 | |||
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Abstract:Effects of silver nitrate on callus regeneration and activity of indole-3-acetic acid oxidase and peroxidase, glutathione S-transferase and reductase, aldehyde oxidase, catalase and peroxidase in cells of immature inflorescence of Triticum aestivum L. variety JI135 were investigated. Immature inflorescences with length between 5-10mm were treated with AgNO3 in MS medium in a period of 10 days. The results indicated that callus regeneration was improved by treatment of wheat immature inflorescence with 16 mg/L AgNO3 for 7 days. The activity of indole-3-acetic acid oxidase and peroxidase was reduced obviously by 16 mg/L AgNO3 four days and six days after inoculation respectively, activity of glutathione transferase, glutathione reductase,catalase,and peroxidase were obviously promoted by AgNO3 4 days after the treatment, But changes in activity of the aldehyde oxidase was not significantly influenced by AgNO3. The mechanism of AgNO3 in improving cell regeneration was discussed. | |||
TO cite this article:Xiangru Liao,Lei Tang,Feng Zhang. Effect of silver nitrate on callus regeneration and activity of some enzymes during tissue culture of wheat immature inflorescence[OL].[ 1 November 2005] http://en.paper.edu.cn/en_releasepaper/content/3441 |
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