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1. Identification and characterization of cell wall proteins of a toxic dinoflagellate Alexandrium catenella using 2-D DIGE and MALDI TOF-TOF mass spectrometry | |||
WANG Dazhi,DONG Hongpo,LI Cheng,XIE Zhangxian,LIN Lin,HONG Huasheng | |||
Environmental Science and Techology 30 March 2011 | |||
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Abstract:Cell wall is an important subcellular component of dinoflgaellate cells in regard to various aspects of cell surface associated ecophysiology but the full range of cell wall proteins (CWPs) and their functions remain to be elucidated. This study identified and characterized CWPs of a toxic dinoflagellate, Alexandrium catenella using a combination of 2-D fluorescence difference gel electrophoresis (DIGE) and MALDI TOF-TOF mass spectrometry approaches. Using sequential extraction and temperature shock methods, sequentially extracted CWPs and protoplast proteins were separated from A. catenella, respectively. Comparison between sequentially extracted CWPs labeled with Cy3 and protoplast proteins labeled with Cy5, 120 CWPs were confidentially identified in the 2-D DIGE gel. These proteins gave positive identification of protein orthologues in the protein database by de novo sequence analysis and homology-based search. Majority of the prominent CWPs identified were hypothetical or putative proteins with unknown function or no annotation, while cell wall modification enzymes, cell wall structural proteins, transporter/binding proteins, signaling and defense proteins were tentatively identified in agreement with the expected role of the extracellular matrix in cell physiology. This work represents the first attempt to investigate CWPs of dinoflagellates which provides a potential tool for future comprehensive characterization of CWPs and elucidation of physiological functions of CWPs in dinoflagellates. | |||
TO cite this article:WANG Dazhi,DONG Hongpo,LI Cheng, et al. Identification and characterization of cell wall proteins of a toxic dinoflagellate Alexandrium catenella using 2-D DIGE and MALDI TOF-TOF mass spectrometry[OL].[30 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4419286 |
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