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1. Reconstruction of pig embryos with different lipids - a handmade model | |||
Wang Chengfei,Niu Yingjie,Chi Daming,Zeng Yaqiong,Li Juan | |||
Animal Husbandry, Veterinary Medicine 23 March 2016 | |||
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Abstract:Numerous neutral lipids were stored in cytoplasm of porcine oocytes in the form of lipid droplets (LDs). These LDs play an important role in vitro maturation and embryo development. Reconstructed embryos with different lipids were performed in present study to establish a model for study of LDs. Three groups of reconstructed embryos were obtained by hand-made cloning (HMC) and delipation: W-L: whole LDs in embryos; H-L: half LDs in embryos; R-L: removed LDs in embryos. After chemical activation (0h), reconstructed embryos were cultured in WOWs system for 6 days. The in vitro developmental competence of the three kinds of reconstructed was assessed. Results showed that: each kind of these three groups of embryos can develop to blastocyst stage. Therefore, the embryos with other content of LDs can be reconstructed in future studies. Then, the H-L group embryos, which as a model to study the roles of LDs and mitochondria in early development of embryos, were composed of three parts: a half normal oocyte (stained by MitoTracker Red CMXRos before bisecting) without nucleus, a half of delipated oocyte without nucleus and a donor cell. Following, 1-cell (12h), 2-cell (24h), 4-cell (36h) and blastocyst (144h) were collected to observe the distribution of LDs and mitochondria. The results showed that LDs and mitochondria were successfully tracked from 1-cell to blastocyst stage. Both LDs and mitochondria were only visible in normal cytoplasm at 1-cell stage. At 2-cell stage, mitochondria were uniformly distributed in the cytoplasm in 28% of the total number. But the LDs still not evenly distributed. However, both LDs and mitochondria were dispersed fully at 4-cell and blastocyst stage. In conclusion, reconstructed embryos with different lipids were appropriate models for lipids study, as well as investigate the interaction among lipids, mitochondria and other organelles. ????? | |||
TO cite this article:Wang Chengfei,Niu Yingjie,Chi Daming, et al. Reconstruction of pig embryos with different lipids - a handmade model[OL].[23 March 2016] http://en.paper.edu.cn/en_releasepaper/content/4681821 |
2. Expression of Nerve Growth Factor (NGF), and Its Receptors TrkA and p75 in the Reproductive Organs of Laying Hens | |||
PU Shaoxia,QU Changwei,LI Chunmei | |||
Animal Husbandry, Veterinary Medicine 10 July 2014 | |||
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Abstract:In order to investigate the expression levels of nerve growth factor (NGF) and its receptors (TrkA and p75) in the prehierarchical follicle and oviducts of hens, five 130-day-old laying hens were examined by immunohistochemistry and RT-PCR analyses. NGF and its receptors were expressed in the theca cells and granulosa cells of prehierarchical follicles, and they also expressed in the epithelial cells of oviducts. The expressions of NGF mRNA were discrepant in different parts of the prehierarchical follicle and oviducts. The expression of NGF and p75 mRNA levels in large white follicle (LWF) were high, as well as TrkA in small yellow follicle (SYF). In oviduct, the expression of NGF is highest in infundibulum but lowest in magnum. These results suggest that NGF may play an important role in the regulation of reproduction in hens. | |||
TO cite this article:PU Shaoxia,QU Changwei,LI Chunmei. Expression of Nerve Growth Factor (NGF), and Its Receptors TrkA and p75 in the Reproductive Organs of Laying Hens[OL].[10 July 2014] http://en.paper.edu.cn/en_releasepaper/content/4603982 |
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