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	1. Effect of dietary nitrate dosage on gas production in vitro and rumen bacterial abundance of Hu Sheep
	Chen Zhiyuan,Ma Tingting,Gong Xiaoxiao,Su Xiaoshuang,Li Guodong,Zhao Guoqi,Lin Miao
	Animal Husbandry, Veterinary Medicine 24 August 2015
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:The objective of this study was to evaluate the effect of diet supplementation with potassium nitrate on rumen gas production in vitro and bacterial diversity in vivo in in sheep. First, rumen fluid was obtained from 3 cannulated Hu sheep fed basal diet to compare gas production of in vitro diet contained nitrate 0%(A, control group), 1% (B), 2% (C), 3% (D), 4% (E), 5% (F), respectively. In a second experiment, 3 cannulated sheep were fed the six diets above, with nitrate concentration increased gradually. Each period had 16 days; before morning feeding at the end of each period, rumen fluid was collected for DNA extraction, 16S rRNA Illumina sequencing and qPCR analysis. In vitro study showed that there was no significant difference in the net gas production, theoretical maximum gas volume and speed of gas production among groups, however, the net gas production and theoretical maximum gas volume of 5% group tended to declined(P<0.1). Paired-End sequencing of 16S rRNA gene targeting showed that Chao1 index of 5% group was significantly higher than control. No difference in Shannon index was observed. At the phylum level, the total percentage of Bacteroidetes, Firmicutes, Proteobacteria, Fibrobacteres, Verrucomicrobia was more than 90%. At the genus level, Prevotella, Selenomonas, Succinivibrio were dominant genus in each group, the proportion was 47.87%, 5.76%, 2.37% respectively. qPCR showed increase in the relative abundance of Campylobacter fetus with nitrate concentration increased (P<0.01). These results indicate that nitrate addition ranged from 0% to 5% change the abundance of bacteria, especially nitrate reduction bacteria, but do not change gas production, rumen bacterial diversity and advantages community structure.
	TO cite this article:Chen Zhiyuan,Ma Tingting,Gong Xiaoxiao, et al. Effect of dietary nitrate dosage on gas production in vitro and rumen bacterial abundance of Hu Sheep[OL].[24 August 2015] http://en.paper.edu.cn/en_releasepaper/content/4652935


	2. Zinc Prevents Salmonella enterica Serovar Typhimurium-Induced Loss of Intestinal Mucosal Barrier Function in broiler chickens
	Zhang Bingkun,Shao Yuxin,Guo Yuming,Yuan Jianmin
	Animal Husbandry, Veterinary Medicine 26 April 2012
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	Abstract:The present study was carried out to evaluate the beneficial effects of supplemental zinc (Zn) on the intestinal mucosal barrier function in Salmonella enterica Serovar Typhimurium-challenged broiler chickens in a 42-d experiment. A total of 336 1-day-old male Arbor Acres broiler chicks were assigned to 8 treatment groups. A 4 × 2 factorial arrangement of treatments was used in a completely randomized experimental design to study the effects of levels of supplemental Zn (0, 40, 80, 120 mg/kg of diet), pathogen challenge (with or without S. Typhimurium challenge), and their interactions. S. Typhimurium infection caused reduction of growth performance (P <0.05) and intestinal injury as determined by reduced (P <0.05) villus height/crypt depth ratio and sucrase activity in the ileum, increased (P <0.05) plasma endotoxin levels, and reduced (P <0.05) claudin-1, occludin and MUC2 mRNA expression in the ileum at day 21. Zinc pretreatment tended to improve body weight gain (P = 0.072) in the starter period, increase the activity of ileal sucrase (P=0.077), but tended to reduce plasma endotoxin levels (P=0.080), and significantly increased (P <0.05) villus height/crypt depth ratio and mRNA levels of occludin and claudin-1 in the ileum at day 21. The results indicated that dietary Zn supplementation appeared to alleviate the loss of intestinal mucosal barrier function induced by S. Typhimurium challenge and the partial mechanism might be related to the increased expression of occludin and claudin-1 in broiler chickens.
	TO cite this article:Zhang Bingkun,Shao Yuxin,Guo Yuming, et al. Zinc Prevents Salmonella enterica Serovar Typhimurium-Induced Loss of Intestinal Mucosal Barrier Function in broiler chickens[J].


	3. Paradigm of Time-sequence Development of the Intestine of Suckling Piglets with microarray
	SUN Yunzi,YU Bing,ZHANG Keying,HE Jun,CHEN Xijian,CHEN Daiwen
	Animal Husbandry, Veterinary Medicine 22 February 2011
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	Abstract:The interaction of the genes involved in intestinal development is the molecular basis of the regulatory mechanisms of intestinal development. The objective of this study was to identify the significant pathways and key genes that regulate intestinal development in Landrace piglets, and then to elucidate their rules of operation. The differential expression of genes related to intestinal development during suckling was investigated using a porcine genome array. Time sequence profiles were then analyzed for the differentially expressed genes to obtain significant expression profiles. Subsequently, the most significant profiles were assayed using Gene Ontology categories, pathway analysis, network analysis, and analysis of gene co-expression to unveil the main biological processes, the significant pathways, and the effective genes, respectively. In addition, quantitative real-time PCR was carried out to verify the reliability of the results of the analysis of the array. The results showed that more than 8000 differential expression transcripts were identified using microarray technology. Among the 30 significant model profiles obtained, profiles 66 and 13 were the most significant. Analysis of profiles 66 and 13 indicated that they were mainly involved in immunity, metabolism, and cell division or proliferation. Among the most effective genes in these two profiles, CN161469, which is similar to methylcrotonoyl-Coenzyme A carboxylase 2 (beta), and U89949.1, which encodes a folate binding protein, had a crucial influence on the co-expression network.
	TO cite this article:SUN Yunzi,YU Bing,ZHANG Keying, et al. Paradigm of Time-sequence Development of the Intestine of Suckling Piglets with microarray[OL].[22 February 2011] http://en.paper.edu.cn/en_releasepaper/content/4411626

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