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1. High cell-density production of glucagon-like peptide-1 derivate (GP62) in recombinant E.coli by optimization of culture medium | |||
Jiang Deqi,Ran Yanhong,Chen Ying,Sang Yanxia,Zhou Tianhong,Li Hongjian | |||
Biology 11 February 2011 | |||
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Abstract:This article aims to get high level expression of GLP-1 derivate (GP62) in high cell-density recombinant Escherichia coli BL21(DE3) by optimizing the fed-batch culture medium. The effects of different basal mediums, yeast extract and inorganic salts concentration on cell growth and expression of GLP-1 derivate (GP62) in recombinant E.coli BL21(DE3) were investigated by single factor test and orthogonal array. Based on the 2YT mediun, get the optimun 2YT-GP62 medium. The highest productivity of 30.0 mg l-1 GLP-1 derivate (GP62) and the ?nal cell density 21.3 (OD600) had been obtained in 5L fermentor induced with 0.6 mmol l-1 IPTG. The final cell density was 4.4 times higher than that in LB culture medium and the expression level of GP62 improved from 27.5% to 31.2%. The results showed that 2YT-GP62 medium can be used for recombinant GLP-1 derivate batch cultivation. | |||
TO cite this article:Jiang Deqi,Ran Yanhong,Chen Ying, et al. High cell-density production of glucagon-like peptide-1 derivate (GP62) in recombinant E.coli by optimization of culture medium[OL].[11 February 2011] http://en.paper.edu.cn/en_releasepaper/content/4410546 |
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