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	1. Identification of thidiazuron-induced ESTs expressed differentially during callus differentiation of alfalfa (Medicago sativa L. cv. Jinnan)
	Zhang Chun-Rong ,Huang Xue-Lin ,Wu Jian-Yong ,Feng Bi-Hong,Chen Yunfeng
	Biology 05 December 2005
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	Abstract:Embryogenic callus induced from petioles of alfalfa (Medicago sativa L. cv. Jinnan) and maintained on kinetin (KN)-containing B5h medium becomes non-embryogenic with differentiation of tracheary elements when kinetin in the medium is replaced by thidiazuron (TDZ). To investigate the molecular mechanisms underlying the TDZ-induced morphological competence transition, we cloned the differentially expressed sequence tags (ESTs) from TDZ-treated callus of alfalfa using suppression subtractive hybridization (SSH). The expression patterns of genes induced by TDZ were found to change throughout the process of TDZ treatment. A homeodomain-leucine zipper protein gene, MSHB1, which was induced by TDZ, may be a transcription factor in the TDZ-regulation of alfalfa callus differentiation. The induction of trehalose-6-phosphate phosphatase (TPP), which may decrease the level of trehalose-6-phosphate, and the induction of 1-aminocyclopropane-1-carboxylate synthase (ACS), which increases the level of ethylene, by TDZ may be responsible for the termination of somatic embryogenesis.
	TO cite this article:Zhang Chun-Rong ,Huang Xue-Lin ,Wu Jian-Yong , et al. Identification of thidiazuron-induced ESTs expressed differentially during callus differentiation of alfalfa (Medicago sativa L. cv. Jinnan)[OL].[ 5 December 2005] http://en.paper.edu.cn/en_releasepaper/content/4069


	2. Comparative studies of senescence-related enzymes in the cotyledon of chlorophyll b–deficient mutant and its wild type oilseed rape during senescence
	Tingting Qin,Jingfeng Fu,Nianhui Zhang,Linfang Du
	Biology 28 November 2005
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	Abstract:The change patterns of senescence-related enzymes during cotyledon senescence were studied in a chlorophyll (Chl) b-deficient mutant type (MT) and its wild type (WT) of Brassica napus L. seedlings. The fresh weight on the basis of cotyledon number initially increased till 20 days after planting (DAP) and then kept relative constant. The protein content decreased sharply since 20 DAP and Chl content reduced since 10 DAP in both two types; however the rate of degradation in protein and Chl in the MT was slower than that in the WT since 20 DAP. Superoxide dismutase (SOD; E.C.1.15.1.1) activity declined in the WT but increased in the MT since 20 DAP. Activity of peroxidase (POD; E.C.1.11.1.7) increased markedly after 20 DAP in both types. Esterase (EST; E.C.3.1.1.1) activity increased in both two types since 10 DAP, whereas at 40 DAP it was much lower in the MT than that in the WT. In addition, bands patterns of SOD, POD and EST isozymes were changed during cotyledon development in both two types, but some differences were observed. Cu/ZnSODs activities were higher in the MT at 40 DAP as compared with the WT. These results showed that day 20 was the turning point during the cotyledon development and the senescence in Cr3529 cotyledon was slower than that in the WT.
	TO cite this article:Tingting Qin,Jingfeng Fu,Nianhui Zhang, et al. Comparative studies of senescence-related enzymes in the cotyledon of chlorophyll b–deficient mutant and its wild type oilseed rape during senescence[OL].[28 November 2005] http://en.paper.edu.cn/en_releasepaper/content/3804


	3. Effect of gibberellic acid and abscisic acid pretreatment on seedling growth and α-amylase activity in endosperms of wheat
	Xiangru Liao,Qun Sun,Xuejun Li,Junfeng Gao
	Biology 04 November 2005
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	Abstract:eeds of Triticum aestivum L. cv. Zhengyin No1 were treated by 10 μmol/L GA3 and 10 μmol /L ABA before germination respectively. The growth of root and shoot, contents of endosperm amylose and soluble carbohydrate, medial pH value, the utilizing efficiency of storage materials (MUE), and activity of α-amylase in endosperm during seed germination under osmotic stress were investigated. The results showed that shoot growth was promoted by GA3 and inhibited by ABA and osmotic stress. Root growth was promoted by GA3, and retarded by osmotic stress and ABA at early stage. But the growth of root of seedlings from seeds treated by ABA was promoted significantly during the late germination stage. Seedling growth was positively correlated with medial pH and MUE significantly. GA3 promoted the expression and increase in activity of α-amylase in endosperm of wheat seed, resulting in increase in breakdown of amylose to soluble carbohydrates, but ABA and osmotic stress had the opposite effect to GA
	TO cite this article:Xiangru Liao,Qun Sun,Xuejun Li, et al. Effect of gibberellic acid and abscisic acid pretreatment on seedling growth and α-amylase activity in endosperms of wheat[OL].[ 4 November 2005] http://en.paper.edu.cn/en_releasepaper/content/3504


	4. Collapse of Water-Stress Emboli in the Tracheids of Platycladus Orientalis L.
	Shen Fanyi,Guo Rui,Gao Rongfu
	Biology 28 January 2005
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	Abstract:e did the same experiment as Lewis’ (1994), but came out with different kinetics of embolus collapse in the tracheids of stem segments of Platycladus Orientalis L.. Tangential wood sections were trimmed. They were observed under a microscope, while segments were dehydrated and rehydrated. As in Lewis’ experiment, following the initial contract of the gas in the tracheids of a segment, the elongated bubbles began to shrink. Then, we found that there were spherical bubbles between coverslip and glass slide, and the bigger ones were getting larger and the smaller ones contracted gradually while the elongated bubbles in the tracheids collapsed. By calculation, the total amount of gas in all bubbles at the beginning of the collapse of the elongated bubbles was almost the same as the amount of gas midway and at end. It has been known that when two spherical bubbles in an identical ambience connect the gas in the smaller one will pass into the larger one, and the bigger one will get larger a
	TO cite this article:Shen Fanyi,Guo Rui,Gao Rongfu. Collapse of Water-Stress Emboli in the Tracheids of Platycladus Orientalis L.[OL].[28 January 2005] http://en.paper.edu.cn/en_releasepaper/content/1509

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