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1. Study on the interaction locations between Humic acid and Pyrene | |||
MA Xingke,PENG Jinghe,ZHU Yaxian,WU Fang,ZHANG Yong | |||
Environmental Science and Techology 05 February 2012 | |||
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Abstract:IFluorescence quenching method was employed to determine the binding constant (Koc) of Humic acid (HA) - Pyrene (Py). The effects of pH value, ionic strength and heavy metal (HM) ions on the Koc were investigated and used to discuss the interaction mechanism between HA and Py. A fluorescent probe method was established and firstly applied to study the interaction between HA and Py by investigating the variation of I1/I3 with the chemical conditions of HA solution. Experimental results indicated that I1/I3 values showed an opposite trend compared to that of Koc, but an identical explanation on the interaction between HA and Py was obtained. The effects of HM ions on the interaction between HA and Py were dependent on the binding sites of HA and HM ions which was located at the carboxyl and hydroxyl. 1H NMR was applied to search for the binding site between HA and Py which was located at aliphatic chain of the HA.n this paper. | |||
TO cite this article:MA Xingke,PENG Jinghe,ZHU Yaxian, et al. Study on the interaction locations between Humic acid and Pyrene[OL].[ 5 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4465128 |
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