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	1. Aluminum-induced changes in reactive oxygen species accumulation, lipid peroxidation and antioxidative capacity in root tips of two wheat genotypes differing in aluminum tolerance
	Xu Fangjie,Li Gang,Jiang Sisi,Zhang Yongsong,Lin Xianyong
	Agronomy 29 December 2009
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	Abstract:
	TO cite this article:Xu Fangjie,Li Gang,Jiang Sisi, et al. Aluminum-induced changes in reactive oxygen species accumulation, lipid peroxidation and antioxidative capacity in root tips of two wheat genotypes differing in aluminum tolerance[OL].[29 December 2009] http://en.paper.edu.cn/en_releasepaper/content/38216


	2. Isolation and Characterization of a Phytase Gene (Sphy1) from Soybean (Glycine max (L.) Merr.)
	Guo Li,Wang Jiaojiao,Xiao Kai
	Agronomy 17 December 2009
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	Abstract:A novel phytase gene Sphy1 was isolated based on screening a cDNA library which was constructed from germinated soybean (Glycine max (L.) Merr. cv. Kefeng6) cotyledon. The full-length cDNA of Sphy1 was 1 644 bp predicated to encode 547 amino acids including an N terminal signal peptide of 27 amino acids. Phylogenetic analysis indicated that Sphy1 had high similarities with the phytase genes from M. truncatula and rice, and acid phosphatase genes from M. truncatula and Arabidopsis. Prokaryotic expression of Sphy1 in BL21 showed that the induced protein had high phytase activities. The transcripts of Sphy1 could be detected in various tissues, such as cotyledons, leaves, stems and roots of seedlings. The phytase activities in the above tissues were accordance with their corresponding Sphy1 transcripts. The transcripts of Sphy1 in cotyledons showed an increasing trend from 5 to 30 days after germination, suggesting that Sphy1 had involved the hydrolyses of the organic phosphorus compounds in seeds from the stages of early seed germination to young seedlings in soybean. Therefore, it is speculated that Sphy1 plays an important role during the seed germination and the growth of the seedlings by releasing inorganic phosphorus (Pi) from phosphorus reserve in seeds.
	TO cite this article:Guo Li,Wang Jiaojiao,Xiao Kai. Isolation and Characterization of a Phytase Gene (Sphy1) from Soybean (Glycine max (L.) Merr.)[OL].[17 December 2009] http://en.paper.edu.cn/en_releasepaper/content/37665


	3. Nitrogen fixation of faba bean interacting with a non-legume in two contrasting intercropping systems
	Fan Fenliang,Yu Changbing,Sun Jianhao,Li Long
	Agronomy 28 December 2007
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	Abstract:A field experiment was carried out to quantify biological nitrogen fixation (BNF) using the 15N isotope natural abundance method in maize (Zea mays L.) / faba bean (Vicia faba L.) and wheat (Triticum aestivum L.) /faba bean intercropping systems. Faba bean was yielding more in the maize/faba bean intercropping, but not in the wheat/faba bean intercropping. Biomass, grain yield and nitrogen acquisition of faba bean were significantly increased when intercropped with maize, and decreased significantly with wheat, irrespective of N-fertilizer application, indicating that the legume could gain or lose productivity in an intercropping situation. N fertilization inhibited nitrogen fixation of faba bean in intercropping with either maize or wheat, and when used in monoculture. The response of different cropping system to N-fertilizer application, however, was not identical, with competitive intercropping (wheat/faba bean) being more sensitive than facilitative intercropping (maize/faba bean). Intercropping increased the proportion of nitrogen derived from air (%Ndfa) of the wheat/faba bean system, but not that of the maize/faba bean system when no N fertilizer was applied. When receiving 120 N kg/ha, however, intercropping did not significantly increase %Ndfa either in the wheat/faba bean system or in the maize/faba bean system in comparison with faba bean in monoculture. The amount of nitrogen derived from air (Ndfa), however, increased significantly when intercropped with maize, irrespective of N-fertilizer application. Ndfa decreased when intercropped with wheat, albeit not significance at 120 kg N/ha. Ndfa correlated more closely with dry matter yield, grain yield and competitive ratio, than with %Ndfa. This indicates that not %Ndfa, but total dry matter yield (sink strength) was more critical for the legume to increase Ndfa. The results suggested that nitrogen fixation could be improved by yield maximization in an intercropping system.
	TO cite this article:Fan Fenliang,Yu Changbing,Sun Jianhao, et al. Nitrogen fixation of faba bean interacting with a non-legume in two contrasting intercropping systems[OL].[28 December 2007] http://en.paper.edu.cn/en_releasepaper/content/17521


	4. Amelioration of nitrogen difference method in legume intercropping system
	Yu Changbing,Li Long
	Agronomy 28 December 2007
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	Abstract:Nitrogen difference method is commonly used to appraise biological nitrogen fixation, which is a well established aspect of plant condition. In an intercropping system, belowground interspecific interactions affected the reliability of this method, so a field experiment was conducted to ameliorate this problem. A split-plot experiment was designed, with the main plot treatments of 0 and 225 kg N ha-1, and sub-plot treatments consisting of sole maize, sole faba bean, sole pea and sole soybean, and faba bean, pea and soybean intercropping with maize. Biomass of intercropping faba bean and pea was found to be increased, but soybean decreased significantly. Biomass of intercropping maize increased significantly than grown alone. Adding fertilizer nitrogen had no marked effect on biomass of the legumes, but that of maize increased. Aggressivity of faba bean (1.06~1.56) and pea (0.11~0.76) were always significantly greater than zero, but it was significantly decreased for soybean (-1.17~-0.81) than maize. Proportion of biological nitrogen fixation %Ndfa) increased for intercropping faba bean and pea than sole, and it was decreased for soybean due to a difference between legume and reference plant. Add nitrogen could decrease %Ndfa significantly for all legumes. An ameliorated nitrogen difference method was established, which takes into account the difference of nitrogen loss and interspecific nutrient competition. Compared with traditional methods, the %Ndfa of intercropping faba bean decreased significantly, and %Ndfa of intercropping pea decreased also, but %Ndfa of soybean increased. Since consider the nitrogen loss and interspecific nitrogen competition and transfer, the ameliorated method of NDM could appraise the proper %Ndfa of intercropping legumes more accurately.
	TO cite this article:Yu Changbing,Li Long. Amelioration of nitrogen difference method in legume intercropping system[OL].[28 December 2007] http://en.paper.edu.cn/en_releasepaper/content/17515


	5. Regulation of Root Cluster Formation and Citrate Exudation in White Lupin (Lupinus albus L.) Plants
	LI Chun-Jian,LIANG Rui-Xia
	Agronomy 05 December 2005
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	Abstract:Split-root system was used to investigate whether the external or internal P concentration controls root cluster formation and citrate exudation in white lupin (Lupinus albus L.) grown under the controlled conditions. In spite of low P concentration in shoot and root of the -P plant, its dry weight was not reduced, compared with the +P plant. Supplying external P (0.25 mmol/L) to one root half resulted in increase in P concentration not only in the shoot, but also in the P deprived root half, indicating P cycling within the plants. P omitting from both split-root pots stimulated root cluster formation in both root halves. While P supply to one root half stimulated root cluster formation at the beginning of the treatment. However, neither P supply to just one root half continuously nor re-supply of P to one root half after 19 d of P starvation could inhibited root cluster formation in the P deprived side, although the P concentration in this root half and shoot increased markedly. The results indicated that root cluster formation in Lupinus albus was controlled by both shoot and root P concentration. The rates of citrate exudation by both root halves with P-deficiency were higher than that of one root half supplied with P only. In the treatment with one root half supplied with P, the rates of citrate exudation by either the P-supplied or deprived root halves were almost same regardless of P concentration in the roots. The results suggested that internal P concentration controlled root cluster formation and citrate exudation in white lupin, but they might be regulated by different mechanisms.
	TO cite this article:LI Chun-Jian,LIANG Rui-Xia. Regulation of Root Cluster Formation and Citrate Exudation in White Lupin (Lupinus albus L.) Plants[OL].[ 5 December 2005] http://en.paper.edu.cn/en_releasepaper/content/4064

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