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1. Non-target-site based metribuzin tolerance in two induced mutants of narrow-leafed lupin (Lupinus angustifolius L.) | |||
PAN Gang,SI Ping,YU Qin,POWLES Steve,TU Jumin | |||
Agronomy 21 January 2011 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:Lupin (Lupinus angustifolius L.) is the most important grain legume crop in Australia. Metribuzin is widely used to control weeds in lupin crops. This study investigates metribuzin tolerance mechanism in narrow-leafed lupin (Lupinus angustifolius L.) using two tolerant mutants Tanjil-AZ-33 and Tanjil-AZ-55 in comparison to the susceptible wild type cv Tanjil. Sequencing of the highly conserved region of the chloroplast psbA gene that covers all the potential herbicide resistance-endowing mutations revealed that the sequences between the wild type and mutants are identical and no known resistance psbA mutations were identified. This lack of resistance mutations in the psbA gene, together with the nuclear inherited tolerance pattern determined in our previous study, indicate that tolerance in the two mutants is likely non-target site based. Additionally, in contrast to the susceptible plants, the photosynthetic rates of the two tolerant mutants were reduced to 70% of the control 0.5 days after metribuzin treatment, but recovered to the level of the control within 2.5 days of the treatment. The initial reduction and later recovery in photosynthetic rate of tolerant mutants further indicates that target site chloroplast is susceptible and mechanism of tolerance is non-target site based. Investigation of cytochrome P450 inhibitors on plant survival revealed that tolerance in the two mutants was reversed by all three P450 inhibitors (omethoate, malathion and phorate), suggesting P450-mediated metabolism likely to be the mechanism endowing metribuzin tolerance. Interestingly, the GST inhibitor tridiphane reversed tolerance of only one mutant Tanjil-AZ-55, not Tanjil-AZ-33. The differences of the two mutants in response to tridiphane suggest that different metabolic resistance mechanisms are likely involved in tolerance of the two mutants. | |||
TO cite this article:PAN Gang,SI Ping,YU Qin, et al. Non-target-site based metribuzin tolerance in two induced mutants of narrow-leafed lupin (Lupinus angustifolius L.)[OL].[21 January 2011] http://en.paper.edu.cn/en_releasepaper/content/4407785 |
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