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1. Inhibitory affect of epigallocatechin gallate on ovarian cancer proliferation associated with aquaporin 5 expression | |||
Yan Chunxiao,Yang Jianhua,Chen Xuejun | |||
Clinical Medicine 10 December 2010 | |||
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Abstract:Objective. To investigate the effect of Epigallocatechin gallate (EGCG) on ovarian cancer cell line SKOV3 growth and aquaporin 5 (AQP5) expression as well as its possible mechanisms. Method. SKOV3 cells were treated with different concentrations of EGCG and the NF-κB-specific inhibitor pyrrolidine dithiocarbamate (PDTC) for different times. Cell proliferation was determined using the MTT assay, cell apoptosis was evaluated using the DNA ladder assay, the expression of AQP5, NF-κB p65 and IκBα was detected by immunohistochemistry, western blot analysis and RT-PCR, and the correlation of these protein expression was analyzed. Result. With increasing concentrations of EGCG and prolonged treatment times, the growth inhibition rate of SKOV3 cells gradually increased in a dose- and time-dependent manner. The expression of AQP5 and nuclear p65 and IκBα was significantly decreased (P<0.01). The cytoplasmic expression of IκBα gradually increased (P<0.05), and the apoptosis of SKOV3 cells was induced as evidenced by typical fragmentation pattern in a DNA ladder assay. With increasing concentrations of PDTC and prolonged treatment times, the protein and mRNA levels of AQP5 in SKOV3 cells decreased (P<0.01). In addition, the growth inhibition rate of SKOV3 cells significantly increased in a dose- and time-dependent manner. Conclusion. these data show that EGCG inhibited the proliferation and induced the apoptosis of ovarian cancer SKOV3 cells. EGCG also down-regulated expression of AQP5, which may inhibit tumor growth and be associated with nuclear transcription factor NF-κB. | |||
TO cite this article:Yan Chunxiao,Yang Jianhua,Chen Xuejun. Inhibitory affect of epigallocatechin gallate on ovarian cancer proliferation associated with aquaporin 5 expression[OL].[10 December 2010] http://en.paper.edu.cn/en_releasepaper/content/4395830 |
2. Malignant ascites activates NF-κB and promotes proliferation of human ovarian carcinoma (SKOV3) cells | |||
Yang Jianhua,Chen Xuejun,Yan Chunxiao,Shi Yifu | |||
Clinical Medicine 28 July 2010 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:Objective. To investigate SKOV3 cells growth induced by malignant ascites whether to involve in nuclear factor kappaB (NF-κB) signal transduction pathway. Methods. Expression of NF-κBp65 and IκBα protein of SKOV3 cells stimulated by malignant ascites, benign and borderline cystic fluid are measured by immunocytochemistry and western blotting analysis. Results. SKOV3 growth rate in the malignant ascites group (217.24±12.64) is significantly higher than that of the borderline fluid group (147.67±10.43, P=0.000), the borderline fluid group higher than the benign fluid group (57.47±2.30, P=0.000). Immunohistochemical and western blotting analyzing study show that NF-κBp65 and IκBα protein are localized in cytoplasm and karyon. Nuclear-cytosol NF-κBp65 and cytosol NF-κBp65/IκBα protein ratio in the malignant group (12.71±1.70, 3.50±0.74, respectively) is higher than that of the borderline group (2.89±0.84, P=0.000; 1.88±0.44,P=0.015, respectively), as well as the benign group (1.64±0.61, P=0.000; 1.04±0.31,P=0.001, respectively) and the control group (1.23±0.45, P=0.000; 0.69±0.09, P=0.001, respectively). There is a positively correlated relationship between expression of NF-κBp65 and IκBα (correlated coefficient 0.839, P=0.001), between SKOV3 growth rate and nuclear-cytosol NF-κBp65 protein ratio(correlated coefficient 0.878,P=0.000) as well as cytosol NF-κB/IκBα protein ratio (correlated coefficient 0.839,P=0.001). Conclusion. Malignant ascites may activate NF-κB and promote cells proliferation of human ovarian carcinoma SKOV-3 cells. | |||
TO cite this article:Yang Jianhua,Chen Xuejun,Yan Chunxiao, et al. Malignant ascites activates NF-κB and promotes proliferation of human ovarian carcinoma (SKOV3) cells[OL].[28 July 2010] http://en.paper.edu.cn/en_releasepaper/content/4380049 |
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