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	1. Molecular identification, polymorphism and association to Vibrio anguillarum infection of major histocompatibility complex class IIAof half-smooth tongue sole (Cynoglossus semilaevis)
	WANG Xubo,LI Chunmei,QI Jie,WANG Zhigang,YU Haiyang
	Biology 22 July 2011
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	Abstract:Major histocompatibility complex class II antigens are important in vertebrate immune system. In the present study, the full length cDNA sequence of class II A gene was got by RACE-PCR from half-smooth tongue sole (Cynoglossus semilaevis), and its open reading frame (ORF) polymorphism was studied. The whole cDNA sequence was 992 bp in length, including the ORF of 717 bp. Twenty-five alleles were identified and clustered into two distinct groups for the specific nucleotides/ amino acids in specific positions, so they belonged to two class II A genes and dominated as Cyse-DAA and Cyse-DBA with 11 and 14 alleles respectively. Four Cyse-DAA alleles were observed in one individual, and three to five Cyse-DBA alleles in each of three detected individuals, which indicated that at least two loci existed in each gene. Moreover, in order to study the association of the alleles of A genes and resistance to infection, 200 individuals were intraperitoneally injected with Vibrio anguillarum and the first 20 dead individuals and 20 surviving ones were selected for genotypes of A genes. Fifty-six alleles were identified among the 40 individuals, 29 of which belonged to Cyse-DAA and 27 belonged to Cyse-DBA. Eighteen alleles were selected for the association study between alleles and resistance to infection. Though not significant, allele Cyse-DAA0201, Cyse-DAA1101, Cyse-DBA0401, Cyse-DBA1102, Cyse-DBA1801 and Cyse-DBA2201 appeared only in surviving individuals, while allele Cyse-DAA0901, Cyse-DBA1101 and Cyse-DBA*1401 occurred more frequently in dead individuals. This study confirmed the existence and polymorphism of two class II A genes and also the association between alleles of class II A genes and disease susceptibility/ resistance in half-smooth tongue sole.
	TO cite this article:WANG Xubo,LI Chunmei,QI Jie, et al. Molecular identification, polymorphism and association to Vibrio anguillarum infection of major histocompatibility complex class IIAof half-smooth tongue sole (Cynoglossus semilaevis)[OL].[22 July 2011] http://en.paper.edu.cn/en_releasepaper/content/4435563


	2. Major histocompatibility complex II B allele polymorphism and its association with Vibrio anguillarum infection in half-smooth tongue sole
	WANG Xubo,LI Chunmei,QI Jie,WANG Zhigang,YU Haiyang
	Biology 14 July 2011
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	Abstract:Major histocompatibility complex (MHC) class II B molecules play an important role in the adaptive immune response in fish. Previous study has reported that two highly polymorphic class II B genes, Cyse-DAB and Cyse-DBB exist in half-smooth tongue sole (Cynoglossus semilaevis). In this study, the polymorphism within exon 2 of the class II B genes following bacterial challenge was evaluated. Two hundred individuals were injected intraperitoneally with Vibrio anguillarum. Muscle tissue from the first 20 dead and 20 of the survivors was collected for genotyping. Sixty alleles from the 40 individuals were isolated, of which 32 belonged to Cyse-DAB and 28 belonged to Cyse-DBB. The rate of dN (non-synonymous substitution) was higher than that of dS (synonymous substitution) in the PBRs (peptide binding residues) of both class II B genes. Conversely, the rate of dS was higher than dN in the non-PBRs and the complete exon 2 sequence. Thus, the results suggest that positive selection has occurred in the PBRs and purifying selection in the non-PBRs and exon 2. Thirteen class II B alleles were used to study the association between alleles and resistance to infection. Though not significant, alleles Cyse-DAB0601, Cyse-DAB0706, and Cyse-DBB0101, Cyse-DBB1301 were only found in surviving individuals and may represent alleles that have resistance against V. anguillarum infection. Alleles Cyse-DAB0701 and Cyse-DAB1301 were significantly more prevalent in dead individuals than in surviving ones and may represent alleles that are associated with increased susceptibility to V. anguillarum infection.
	TO cite this article:WANG Xubo,LI Chunmei,QI Jie, et al. Major histocompatibility complex II B allele polymorphism and its association with Vibrio anguillarum infection in half-smooth tongue sole[OL].[14 July 2011] http://en.paper.edu.cn/en_releasepaper/content/4435459


	3. Origin and differentiation of a special fragment from Capra hircus agouti gene
	Weiwei WU,Xianglong LI,Rongyan ZHOU,Lanhui LI,Huiqin ZHENG
	Biology 15 March 2010
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	Abstract:A special fragment, 136 bp fragment previously considered only existed in Capra hircus agouti gene but not in agouti gene of cow, sheep, pig, and horse, was analyzed using BLAST program in NCBI and some powerful molecular biology softwares. The results indicated that the special fragment existed in different regions of the agouti gene of Capra hircus and Ovis aries, but not in the agouti gene of Bos taurus. Meanwhile, it appeared in coding or non-coding regions with forward and reverse sequence in other genes of Ovis aries and Bos taurus. The number of forward sequences was higher than that of reverse ones in Bos taurus, but with similar genetic diversity. While the reverse sequence from Ovis aries had higher genetic diversity than forward ones. The differentiation between Bos taurus and Capra hircus was more obvious than between Bos taurus and Ovis aries, but not within species. The BACR5 (AC150540) from Bos taurus and OEEF2 (EE751186) from Ovis aries could be considered as the farmost ancestral sequences.
	TO cite this article:Weiwei WU,Xianglong LI,Rongyan ZHOU, et al. Origin and differentiation of a special fragment from Capra hircus agouti gene[OL].[15 March 2010] http://en.paper.edu.cn/en_releasepaper/content/40689

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