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1. Bioinformatics analysis of tyrosinase-related protein 1 gene (TYRP1) from different species | |||
Huiqin Zheng,Xianglong Li,Rongyan Zhou,Lanhui Li,Xiuli Guo,Jingfen Kang,Dongfeng Li | |||
Biology 25 January 2010 | |||
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Abstract:As one member of tyrosinase-related family directly involved in the production of melanin, TYRP1 is involved in not only melanogenesis but also prevention of melanocyte death, stabilizing tyrosinase and helping determine the shape of melanosomes, etc. Multi-species sequence comparisons showed that there were two evolutionally conserved non-coding regions (from -1306 to -733 and from -642 to -515 according to AL138753) upstream of translational initiation sites, representing putative regulatory regions subject to subsequent experimental test. CDS length variation and genetic diversity analysis showed that Felis catus, Homo sapiens and Canis familiaris had more genetic diversities than the other species for TYRP1, especially Felis catus that could be a better choice for studying the TYRP1-associated genetic basis underlying the color diversity. As a 75 kDa type-1 transmembrane glycoprotein, Mature TYRP1 possesses about 17 kDa modifying components, whose function predominantly depends on the existing glycosyl- groups and the Cu components. In addition, the mutated amino acids within species and the highly conserved amino acids among species were listed in our paper. | |||
TO cite this article:Huiqin Zheng,Xianglong Li,Rongyan Zhou, et al. Bioinformatics analysis of tyrosinase-related protein 1 gene (TYRP1) from different species[OL].[25 January 2010] http://en.paper.edu.cn/en_releasepaper/content/39346 |
2. ESTs involved in interaction between genomic and plasmid DNA in E. coli JM109 | |||
Yao Xinling,Ma Yijing,Zhiping Hong,Qin Wu,Aiguang Guo | |||
Biology 14 January 2010 | |||
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Abstract:An essential issue for genome annotation is to identify genes involving in interaction between genomes in the same cell. It is difficult to do so in eukaryotic cells, because each type of genome is required for cell survival. Escherichia coli, however, is a suitable model for the study of specific gene interactions, because E. coli can grow in the presence or absence of a plasmid. In this paper, genes that are expressed in a cell-specific manner were tested using E. coli JM109 with or without plasmid with modified suppression subtractive hybridization, named transcript subtractive hybridization (TSH). Four out of five genes that were specific to the plasmid were detected by the SSH. Meanwhile, 10 expressed sequence tags (ESTs) were identified that may be involved in interaction between the JM109 genome and the plasmid. This technique provides a potential approach for the study of interaction between genomes in prokaryotes. | |||
TO cite this article:Yao Xinling,Ma Yijing,Zhiping Hong, et al. ESTs involved in interaction between genomic and plasmid DNA in E. coli JM109[OL].[14 January 2010] http://en.paper.edu.cn/en_releasepaper/content/38944 |
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