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1. Recovery Measures and Field Management Strategies After Hailstone Damage in Upland Cotton | |||
NIE Hushuai#,SU Ying#,LI Le,DAI Maohua,WANG Dingwei,SU Beibei,XIAO Feng,HUA Jinping | |||
Agronomy 19 November 2021 | |||
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Abstract:Hailstorm might damage cotton plants severely and cause heavy economic loss in field production. It occurred often in Yellow River Basin Cotton Region and Northwest Inland Cotton Area in China. In 2015 and 2016, there were three times\' hailstorm damaged our field experiments of upland cotton (Gossypium hirsutum L.) in Hebei Province during seedling and bud periods. To reduce losses, we started the study to evaluate the damage effects of hailstone on plant architecture, and performed serials strategies to recover the plants and to rescue the experiments. Based on evaluating the hailstone damage, we fertilized 112.50 kg/ha Urea fertilizer (N content ≥ 46.4%) and intertilled the field timely. The recovery of plant individuals sprouted new buds and many new leaves after 12 DAH. Then the unnecessary shoots were pruned to adjust the development of fruit branches. Normally three new sympodia branches (NSB) in the major stem were reserved and eight subsequent emerging boll branches (EBB) in cotton plants. Five accessions with varied recovery ability and different yield potential were sampled to compare the yield and quality after hail damage. Among them, Sumian 20, 11-0710 and 11-0516 increased, while the other two accessions decreased relative to the normal production without hail damage. BC and RIL populations of upland cotton were used to evaluate the damage ratio of yield and quality, which resulted in yield loss ranged 13.45%-20.27%. Fiber length, fibre elongation, fibre uniformity, and fiber strength decreased slightly in the five accessions and two populations. These results proved that the cotton plants can compensate by proper field managements, and remedial output could be obtained after hail damage. | |||
TO cite this article:NIE Hushuai#,SU Ying#,LI Le, et al. Recovery Measures and Field Management Strategies After Hailstone Damage in Upland Cotton[OL].[19 November 2021] http://en.paper.edu.cn/en_releasepaper/content/4755796 |
2. Identification of different cytoplasms based on newly developed mitotype-specific markers for marker-assisted selection breeding in cultivated Brassica napus | |||
HENG Shuangping,CHEN Fengyi,YANG Zonghui,HU Kaining,WEI Chao,WEN Jing,YI Bing,MA Chaozhi,TU Jinxing,SI Ping,FU Tingdong,SHEN Jinxiong | |||
Agronomy 30 September 2015 | |||
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Abstract:Mitotype-specific markers have been developed in order to distinguish among different mitotypes in plant. Here, we comparatively analyzed six sequenced mitochondrial genomes that are studied in Brassica napus and identified the collinear blocks and mitotype-specific sequences (MSSs) of these mitochondrial genomes. The collinear relationship between mitochondrial genomes of nap, cam, and pol cytoplasmic male sterility (CMS) lines were higher than those of other lines. After comparative analysis of the six sequenced mitochondrial genomes (cam, nap, ole, pol CMS, ogu CMS, and hau CMS), 90 MSSs with sizes ranging from 101 to 9981 bp and a total length of 103,756 bp (accounting for 6.77% of the mitochondrial genome sequences) were identified. Additionally, 12 mitotype-specific markers were developed based on the mitochondrial genome specific sequences in order to distinguish among these different mitotypes. Cytoplasms of 570 different inbred lines collected from various scientific research institutes in China were identified using the MSS markers developed in our study. In addition to confirming the accuracy of the cytoplasmic identification, we also identified mitotypes that have not been reported in Brassica napus. Our study may provide guidance for the classification of different mitotypes in B. napus breeding. | |||
TO cite this article:HENG Shuangping,CHEN Fengyi,YANG Zonghui, et al. Identification of different cytoplasms based on newly developed mitotype-specific markers for marker-assisted selection breeding in cultivated Brassica napus[OL].[30 September 2015] http://en.paper.edu.cn/en_releasepaper/content/4656522 |
3. The arrest of megagametophyte development results in the female sterility of a dominant genic male-sterile line in Brassica napus | |||
Han Xue,Hong Dengfeng,Yang Guangsheng | |||
Agronomy 04 January 2013 | |||
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Abstract:The phenomenon of female sterility on all male-sterile plants was observed in a genic male-sterile (GMS) Brassica napus line designated as FM195AB (A represents sterile plants and B refers to its wild type ones). For the purpose of clarifying the causal mechanism of this female sterility, we contrastively studied the morphological characteristics of reproductive structures and procedures involved in seed formation between FM195A and FM195B. First, Scanning electron microscopy (SEM) investigations on stigma papilla cells showed no apparent difference between the fertile and sterile plants. Second, alien pollen grains can normally germinate and pollen tubes can enter the stigma papilla cells of both FM195A and FM195B; however, pollen tubes in FM195A pistils appear morphologically abnormal in comparison with FM195B, even if they can normally elongate and reach the ovules. Furtther observations of the paraffin-sectioned pistils revealed that early magasporogenesis in FM195A is similar to that of FM195B; however, mono-nucleate and the subsequent developmental stages of the female gametophytes can hardly seen in the ovules of FM195A, suggesting. Approximately at the eight-nucleate embryo sac stage in FM195B, five major types of the abnormal embryo sacs in FM195A can be observed. Similarly, we found that microsporogenesis in FM195A is also stopped before the entrance of meiosis. Because both of the male and female sterility occur at the same plant, it can be assumped that the male sterility gene, Ms, may play an important role in prompting the transition from maintaince of the spore mother cells into meiosis. | |||
TO cite this article:Han Xue,Hong Dengfeng,Yang Guangsheng. The arrest of megagametophyte development results in the female sterility of a dominant genic male-sterile line in Brassica napus[OL].[ 4 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4509274 |
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