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1. Reconstruction of pig embryos with different lipids - a handmade model | |||
Wang Chengfei,Niu Yingjie,Chi Daming,Zeng Yaqiong,Li Juan | |||
Animal Husbandry, Veterinary Medicine 23 March 2016 | |||
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Abstract:Numerous neutral lipids were stored in cytoplasm of porcine oocytes in the form of lipid droplets (LDs). These LDs play an important role in vitro maturation and embryo development. Reconstructed embryos with different lipids were performed in present study to establish a model for study of LDs. Three groups of reconstructed embryos were obtained by hand-made cloning (HMC) and delipation: W-L: whole LDs in embryos; H-L: half LDs in embryos; R-L: removed LDs in embryos. After chemical activation (0h), reconstructed embryos were cultured in WOWs system for 6 days. The in vitro developmental competence of the three kinds of reconstructed was assessed. Results showed that: each kind of these three groups of embryos can develop to blastocyst stage. Therefore, the embryos with other content of LDs can be reconstructed in future studies. Then, the H-L group embryos, which as a model to study the roles of LDs and mitochondria in early development of embryos, were composed of three parts: a half normal oocyte (stained by MitoTracker Red CMXRos before bisecting) without nucleus, a half of delipated oocyte without nucleus and a donor cell. Following, 1-cell (12h), 2-cell (24h), 4-cell (36h) and blastocyst (144h) were collected to observe the distribution of LDs and mitochondria. The results showed that LDs and mitochondria were successfully tracked from 1-cell to blastocyst stage. Both LDs and mitochondria were only visible in normal cytoplasm at 1-cell stage. At 2-cell stage, mitochondria were uniformly distributed in the cytoplasm in 28% of the total number. But the LDs still not evenly distributed. However, both LDs and mitochondria were dispersed fully at 4-cell and blastocyst stage. In conclusion, reconstructed embryos with different lipids were appropriate models for lipids study, as well as investigate the interaction among lipids, mitochondria and other organelles. ????? | |||
TO cite this article:Wang Chengfei,Niu Yingjie,Chi Daming, et al. Reconstruction of pig embryos with different lipids - a handmade model[OL].[23 March 2016] http://en.paper.edu.cn/en_releasepaper/content/4681821 |
2. Expression of Nerve Growth Factor (NGF), and Its Receptors TrkA and p75 in the Reproductive Organs of Laying Hens | |||
PU Shaoxia,QU Changwei,LI Chunmei | |||
Animal Husbandry, Veterinary Medicine 10 July 2014 | |||
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Abstract:In order to investigate the expression levels of nerve growth factor (NGF) and its receptors (TrkA and p75) in the prehierarchical follicle and oviducts of hens, five 130-day-old laying hens were examined by immunohistochemistry and RT-PCR analyses. NGF and its receptors were expressed in the theca cells and granulosa cells of prehierarchical follicles, and they also expressed in the epithelial cells of oviducts. The expressions of NGF mRNA were discrepant in different parts of the prehierarchical follicle and oviducts. The expression of NGF and p75 mRNA levels in large white follicle (LWF) were high, as well as TrkA in small yellow follicle (SYF). In oviduct, the expression of NGF is highest in infundibulum but lowest in magnum. These results suggest that NGF may play an important role in the regulation of reproduction in hens. | |||
TO cite this article:PU Shaoxia,QU Changwei,LI Chunmei. Expression of Nerve Growth Factor (NGF), and Its Receptors TrkA and p75 in the Reproductive Organs of Laying Hens[OL].[10 July 2014] http://en.paper.edu.cn/en_releasepaper/content/4603982 |
3. Nitrate Reduction to Inhibit Ruminal Methanogenesis and to Improve Microbial Nitrogen Synthesis | |||
Meng Qingxiang,Ren Liping,Zhou Zhenming,Lin Miao,Shi Caixia | |||
Animal Husbandry, Veterinary Medicine 13 December 2011 | |||
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Abstract:The majority of research showed that nitrate can be administered to ruminants as a sole or major source of fermentable nitrogen. The research evidence leaves little doubt that nitrate, when included at sufficient concentrations in a diet so as to maintain optimum fermentation rate, can largely prevent enteric methane production and greately improve rumen microbial protein synthesis. Several microbial enzymes are characterised as nitrate and nitrite reductases and often are present in the anaerobic microorganisms. In practice, the limitation to nitrate use in a feed is its association with nitrite poisoning. However, nitrite accumulation appears to only occur when relatively large quantities of nitrate are suddenly introduced directly into the rumen of animals not accustomed to nitrate in their feed. Under normal situations, nitrate is not toxic to ruminant animals, but nitrite is detrimental to well being. The toxicity of nitrate is related to many factors, such as animal type, adaptation period, nitrate dosage, feed type and feed rate. Dissimilatory reduction of nitrate to nitrite and assimilatory reduction of nitrite to ammonia are pathways of nitrate metabolism in the rumen, and multiple nitrate/nitrite reductases are involved in the reduction of nitrate to nitrite then to ammonia. Nitrate addition can inhibit methane production in the rumen with the mechanisms that are inhibition of intermedial by-products during nitrate denitrification, and electron competition between nitrate and nitrite reducing bacteria and methanogenic organisms. Some factors influencing nitrate used in ruminant feeds are also discussed in detail. | |||
TO cite this article:Meng Qingxiang,Ren Liping,Zhou Zhenming, et al. Nitrate Reduction to Inhibit Ruminal Methanogenesis and to Improve Microbial Nitrogen Synthesis[OL].[13 December 2011] http://en.paper.edu.cn/en_releasepaper/content/4455495 |
4. Characterization and SNP Identification of Goat TYRP1 Gene | |||
Zheng Huiqin ,Lanhui Li,Xianglong Li,Rongyan Zhou,Fengling Xuan,Jingjing Zhang,Zunan Shen,Hongbo Zhao | |||
Animal Husbandry, Veterinary Medicine 20 April 2010 | |||
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Abstract:As one member of tyrosinase-related family, tyrosinase-related protein-1(TYRP1) has been reported to play an important role in melanin biosynthetic pathway in some species, but little is known about it in goat. In this study, almost complete goat TYRP1 gene (17554bp) encompassing complete CDS was determined, the coding region was 1614bp, distributed in 7 exons (2-8), intron 5 was found to possess GC-AG as its splice sites. Dozens of SNPs as well as simple sequence repeats (SSR) motifs were detected in the goat TYRP1 gene. Popgene analysis suggested that allele A at site 1263 and haplotype AC for g.1263A>C and g.1428C>T should be in favor of eumelanin biosynthesis, while mutation C→T at site 1428 occurred more recently in evolution and might have little effect on coat color. High deficiency of heterozygotes existed in Nanjiang Yellow Goat Black strain, Nanjiang Yellow Goat Fast Grow strain and Jining Gray Goat, indicating high inbreeding coefficient. Genetic differentiation among the 9 goat populations were significantly low for both g.1263A>C and g.1428C>T. | |||
TO cite this article:Zheng Huiqin ,Lanhui Li,Xianglong Li, et al. Characterization and SNP Identification of Goat TYRP1 Gene[OL].[20 April 2010] http://en.paper.edu.cn/en_releasepaper/content/42134 |
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