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1. Screening of Amylolytic Lactic Acid bacteria for Rice Starch | |||
Nalwoga Mariam,Liu Xiaoming,Zhang Qiuxiang,Zhang Hao,Chen Wei | |||
Food Science and Technology 26 February 2013 | |||
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Abstract:Twenty-nine Lactobacillus Plantarum strains potentially useful in the development of a functional ready to drink rice beverage were screened for amylolytic activity by investigating their capacities to utilize rice starch, using the known amylolytic L. plantarum A6 as a reference strain. Among these strains, 21 were capable of degrading rice starch, 10 showed extracellular amylase activity greater than 0.30 μ Units /ml. The microbial growth, titratable acidity, pH, reducing sugars and organic acids were measured respectively. Only 4 strains were able to grow substantially in the liquid medium, reaching maximum cell count after 12 h fermentation (7.0-10.6 log cfu /ml), with a pH below 4.5. Lactic acid was produced in amounts ranging from 5.98 mg/ml to 8.16 mg/ml and acetic acid ranged between 3.47mg/ml to 5.90 mg/ml. Some strains produced more acetic acid than lactic acid between 12- 24 h of fermentation. L. Plantarum Z4 showed highest residual reducing sugars utilisation content. Two of the strains tested (L. plantarum Z4 and L. plantarum CS) that showed the highest growth and amylase activity were recommended suitable for further studies and the development of a fermented rice beverage. | |||
TO cite this article:Nalwoga Mariam,Liu Xiaoming,Zhang Qiuxiang, et al. Screening of Amylolytic Lactic Acid bacteria for Rice Starch[OL].[26 February 2013] http://en.paper.edu.cn/en_releasepaper/content/4523495 |
2. Effect of microwave on activity and confornation of proteases | |||
Bian Rongxia ,Xiaoming Liu,Daming Fan,Jianxin Zhao,Hao Zhang,Wei chen | |||
Food Science and Technology 20 December 2012 | |||
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Abstract:The use of microwave in food-grade proteases remains limited. This study explored the mechanism through which microwave treatment affects protease activity. Different proteases had various degrees of resistance to microwave, and listing from the strongest to the weakest, the proteases studied were papain, alcalase, bromelain, pepsin, trypsin, flavourzyme, and neutrase. During the microwave treatments, protease activity of papain did not change dramatically through the treatments up to 900 W. On the other hand, the activity of alcalase dropped 75 % at a microwave power of 100 W. In general, with increasing microwave power and length of treatment, protease activity and the α-helix content of the enzymes decreased, and the change in the fluorescence spectra became more obvious. The results indicated that microwave has different influence on the proteases, and microwave has the potential for application in the enzymatic hydrolysis of food. | |||
TO cite this article:Bian Rongxia ,Xiaoming Liu,Daming Fan, et al. Effect of microwave on activity and confornation of proteases[OL].[20 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4505870 |
3. The effect of silencing of LeEIN2 gene in tomato fruit development and ripening | |||
HongLiang Zhu,Ben Zhong Zhu,Yi Shao,Da-Qi Fu,Xiao-Guang Wang,Xi-Jin Lin,Yuan-Hong Xie,Yun-Bo Luo | |||
Food Science and Technology 09 December 2005 | |||
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Abstract:Loss-of-function ETHYLENE INSENSITIVE2 (EIN2) mutations showed ethylene insensitivity in Arabidopsis, which indicated an essential role of EIN2 in ethylene signaling. Recently, the solanaceae EIN2 (petunia EIN2) has also been identified. However, little is known about the role of EIN2 in other solanaceae species such as tomato. To gain a better understanding of EIN2 in tomato, LeEIN2 silenced tomato fruits were developed using a virus-induced gene silencing fruit system to study the role of LeEIN2 in tomato fruit ripening. Silenced fruits had a delay in fruit development and ripening, related to greatly descended expression of ethylene-related and ripening-related genes in comparison with those of control fruits. Therefore, LeEIN2 was proposed to a positive component of the ethylene-signaling pathway in tomato. In addition, there were less seeds and locules in the silenced fruit than the control fruit, like the phenotype of parthenocarpic tomato fruit. The content of auxin and also the expression of auxin-regulated gene were declined in silenced fruit, which indicated the EIN2 might be important for the crosstalk between ethylene and auxin hormones. | |||
TO cite this article:HongLiang Zhu,Ben Zhong Zhu,Yi Shao, et al. The effect of silencing of LeEIN2 gene in tomato fruit development and ripening[OL].[ 9 December 2005] http://en.paper.edu.cn/en_releasepaper/content/4202 |
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