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	1. Magnetic targeting effect of methotrexate-loaded Fe3O4-PLLA-PEG-PLLA microspheres prepared in supercritical CO2
	TANG Na,WANG Shibin,CHEN Aizheng
	Chemical Engineering 13 December 2016
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	Abstract:The magnetic targeting effect of methotrexate-loaded Fe3O4-poly (L-lactide)-poly (ethylene glycol)-poly (L-lactide) magnetic composite microspheres (MTX-Fe3O4-PLLA-PEG-PLLA MCMs, MMCMs) prepared by co-precipitation (C) and microencapsulation (M) in a process of suspension-enhanced dispersion by supercritical CO2 (SpEDS) was evaluated. The observations of movements and aggregations of MMCMs indicate a good magnetic response in vitro. The results of Prussian blue iron-staining in tumor and the distribution of MTX in S-180-bearing KM mice demonstrate that MMCMs with magnetic field induction could effectively target to tumors. The MTX concentration in tumors treated with free MTX, MMCMs(C) and MMCMs(M) were 8.13±0.46 ng/mL, 138.71±66.22 ng/mL and 282.11±9.36 ng/mL, respectively. This reveals that MMCMs(M) with magnetic field induction possessed a much more remarkable targeting effect than MMCMs(C) and free MTX formulations. This study demonstrates that MMCMs(M) could effectively response to the magnetic induction both in vitro and in vivo, and have potential for application in magnetic targeting therapy of solid tumors. ?????
	TO cite this article:TANG Na,WANG Shibin,CHEN Aizheng. Magnetic targeting effect of methotrexate-loaded Fe3O4-PLLA-PEG-PLLA microspheres prepared in supercritical CO2[OL].[13 December 2016] http://en.paper.edu.cn/en_releasepaper/content/4713859


	2. A Microneedle Mold Fabrication Method
	Guo Xin Dong,Wang Qi Lei
	Chemical Engineering 24 November 2015
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	Abstract:Microneedle (MN) offers an attractive, painless and minimally invasive approach for transdermal drug delivery. Polymer microneedles are normally fabricated by using the micromolding method employing a MN mold, which is suitable for mass production due to high production efficiency and repeat-using of the mold. Most of the MN molds are prepared by pouring sylgard polymer over a MN master to make an inverse one after curing, which is limited in optimizing or controlling the MN structures and failing to keep the sharpness of MNs. In this work we describe a fabrication method of MN mold with controlled microstructures, which is meaningful for the fabrication of polymer MNs with different geometries. Laser micro-machining method was employed to drill on the surface of PDMS sheets to obtain MN molds. In the fabrication process, the microstructures of MN molds are precisely controlled by changing laser parameters and imported patterns. The MNs prepared from these molds are sharp enough to penetrate the skin. This scalable MN mold fabrication method is helpful for future applications of MNs.
	TO cite this article:Guo Xin Dong,Wang Qi Lei. A Microneedle Mold Fabrication Method[OL].[24 November 2015] http://en.paper.edu.cn/en_releasepaper/content/4664002


	3. Optimization of trace elements using response surface methodology (RSM) for biogas production in dry anaerobic digestion
	LI Hongli,CAO Feifei,WANG Yan
	Chemical Engineering 25 January 2013
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	Abstract:The effect of trace elements (Fe、Co and Ni) on mesophilic dry anaerobic digestion was investigated in this study. Response surface methodology (RSM), based on a three-level Box-Behnken Design with the Design-Expert Software, was employed in order to obtain the optimal concentrations of trace elements. The experiment results demonstrated that the optimized concentrations of trace elements were 15.0 mg FeoL-1, 1.0 mg CooL-1, 1.2 mg NioL-1, respectively. Under the optimized condition, the maximum accumulated biogas reached 7895.28 mL in mesophilic dry anaerobic digestion, which was consistent with the predicted value by the second-order polynomial model.
	TO cite this article:LI Hongli,CAO Feifei,WANG Yan. Optimization of trace elements using response surface methodology (RSM) for biogas production in dry anaerobic digestion[OL].[25 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4518256


	4. Cloning, expression and characterization of thermostable lipase from Geobacillus stearothermophilus DSMZ 1550
	Hu Sheng,Huang Jun,Wu Hong,Mei Lehe
	Chemical Engineering 27 March 2012
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	Abstract:A lipase gene of about 1.3 kb was cloned and sequenced from Geobacillus stearothermophilus strain DSMZ 1550. Nucleotide sequence homology analysis showed that the target gene sequence was completely identical to a published sequence (GenBank accession no. AF237623) from Bacillus stearothermophilus P1. The lipase gene was cloned into pET-28c (+) to construct an expression plasmid (pET-28c(+)-lipase), which was transformed into E. coli BL21 for expression. The cultivation parameters of the expression vector harboring strain were optimized to produce the lipase efficiently. The optimal conditions were determined as following: induction at middle stage of exponential growth (OD600 1.09) with 0.5 mM isopropylthio-d-galactoside (IPTG), induction temperature 28℃and post-induction expression for 6 h. Under the above conditions, the specific activity of lipase reached 1.89 IU/mg. Some characteristics of crude recombinant lipase were also investigated. The optimum pH and temperature of the crude lipase were 8.5 and 55℃. The enzyme activity was strongly inhibited by Zn2+, Fe2+, and phenylmethylsulfonyl fluoride (PMSF).
	TO cite this article:Hu Sheng,Huang Jun,Wu Hong, et al. Cloning, expression and characterization of thermostable lipase from Geobacillus stearothermophilus DSMZ 1550[OL].[27 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4473260

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