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	1. Optimization of Fermentation Medium of K.rhizophila.H5401 for Producing (R)-Adrenaline Using Response Surface Methodology
	CHEN Bingmei,XU Xiaoping
	Chemical Engineering 18 February 2014
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:A bacterium strain Kocuria rhizophila.XL11 capable of producing (R)-adrenaline ((R)-epinephrine) with high optical purity (enantiomeric excess > 99%) was firstly isolated. Optimization of the fermentation medium was performed by using response surface methodology. Critical nutrients were initially selected according to a Plackett-Burman design, followed by an application of steepest ascent method to approach the optimal response area. The optimized medium composition for maximum adrenaline production by the mutant strain Kocuria rhizophila.H5401 was obtained based on a three-level three-factor Box-Behnken design, and the optimum concentrations of the critical factors were obtained as follows: glycerol 8.61 goL-1, peptone 7.22 goL-1 and NH4H2PO4 5.65 goL-1. The maximum yield of adrenaline was found to be 61.14%, which is an increase of 25.0% compared to that obtained from the original fermentation medium.
	TO cite this article:CHEN Bingmei,XU Xiaoping. Optimization of Fermentation Medium of K.rhizophila.H5401 for Producing (R)-Adrenaline Using Response Surface Methodology[OL].[18 February 2014] http://en.paper.edu.cn/en_releasepaper/content/4583994


	2. Engineering Bacillus subtilis for acetoin production from glucose and xylose mixtures
	CHEN Tao,LIU Weixi,FU Jing,ZHANG BO
	Chemical Engineering 23 April 2013
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:As a vital flavor compound, acetoin is extensively used in dairy products and drinks industry. In this study, Bacillus subtilis was engineered to metabolize glucose and xylose as substrates for acetoin production. Initially, gene araE from B. subtilis, encoding the xylose transport protein AraE, was placed under the control of the constitutive promoter P43 for over-expression. Batch cultures showed that 10 g/L xylose was depleted completely in 32 hours. Subsequently, genes xylA and xylB from Escherichia coli, encoding xylose isomerase and xylulokinase respectively, were introduced into B. subtilis, and the recombinant turned out to assimilate glucose and xylose without preference. In shake-flask fermentations, 5.5 g/L acetoin with a yield of 0.70 mol (molosugar)-1 was obtained by the optimum strain BSUL13 under microaerobic conditions, which offered a metabolic engineering strategy on engineering microbe as cell factory for the production of high-valued chemicals from renewable resource.
	TO cite this article:CHEN Tao,LIU Weixi,FU Jing, et al. Engineering Bacillus subtilis for acetoin production from glucose and xylose mixtures[J].


	3. An oxidoreduction potential shift control strategy for high purity propionic acid production by Propionibacterium freudenreichii CCTCC M207015 with glycerol as sole carbon source
	CHEN Fei,FENG Xiaohai,LIANG Jinfeng,XU Hong
	Chemical Engineering 04 June 2012
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	Abstract:The effects of oxidoreduction potential (ORP) regulation on the process of propionic acid production by Propionibacterium freudenreichii CCTCC M207015 have been investigated. Potassium ferricyanide and sodium borohydride were to determine as ORP control agents in serum bottle. In batch fermentation, cell growth, propionic acid and by-products distribution were changed with ORP levels in the range of 0 to 160 mV. Based on these analysis results, an ORP-shift control strategy was proposed: At first 156 h, ORP was controlled at 120 mV to obtain higher cell growth rate and propionic acid formation rate, then it was shifted to 80 mV after 156 h to maintain the higher propionic acid formation rate. By applying this ORP-shift control strategy, the optimal parameters were obtained as follows: the propionic acid concentration 45.99 g/L, productivity 0.192 g/L/h, the proportion of propionic acid to total organic acids 92.26% (w/w) and glycerol conversion efficiency 76.65%. The mechanism of ORP regulation was discussed by the ratio of NADH/NAD and ATP levels. The results suggest that it is possible to redistribute metabolic fluxes by the ORP-shift control strategy, and the strategy could provide a simple and efficient tool to realize high purity propionic acid production using glycerol.
	TO cite this article:CHEN Fei,FENG Xiaohai,LIANG Jinfeng, et al. An oxidoreduction potential shift control strategy for high purity propionic acid production by Propionibacterium freudenreichii CCTCC M207015 with glycerol as sole carbon source[J].

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