Abstract:Flavonoid intake from vegetables, fruit, berries and beverages has been favorably linked with reduced risks of a number of diseases. One possible mechanism behind the health effects of flavonoids is their antioxidant effects. An abundant intake of antioxidants from the diet may help the body to protect against harmful effects of pollution, UV light, cigarette smoke and radiation caused by free radicals. Okra (Abelmoschus Esculentus) is a vegetable widely grown in the tropics, sub-tropics and warmer areas of the temperate zones. It is known to have significant amounts of flavonoids and phenols. The 13 accessions of Okra were collected from different geographic regions of China. The objective of the study was to assess the difference of total flavonoid contents from the different tissues and varieties of Okra. Results indicated that the flavonoid content of the flowers was quite high compared to that of the root, stem, leaves, leaf stalk, and pod. Among the 13 different Okra varieties, the content of total flower flavoniods of Xiangkui No.3 was the highest. The content of total flavoniods of Xiangkui No.3 in pod was the highest among Xiangkui No.1, 3, 4, 5, 8. The above results provide a new way for the quality assessment and industrialization study of Okra.

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	1. Comparative study of total flavonoid contents from the different tissues and varieties of Abelmoschus Esculentus
	Hu Shuai,Yuan Congying,LI Qiang,Zhang Cheng,Wang Ping,Wan jie,Chang Hongping,Lu xiutao,He Zhuang,Guo Xinhong
	Biology 22 November 2013
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	Abstract:Flavonoid intake from vegetables, fruit, berries and beverages has been favorably linked with reduced risks of a number of diseases. One possible mechanism behind the health effects of flavonoids is their antioxidant effects. An abundant intake of antioxidants from the diet may help the body to protect against harmful effects of pollution, UV light, cigarette smoke and radiation caused by free radicals. Okra (Abelmoschus Esculentus) is a vegetable widely grown in the tropics, sub-tropics and warmer areas of the temperate zones. It is known to have significant amounts of flavonoids and phenols. The 13 accessions of Okra were collected from different geographic regions of China. The objective of the study was to assess the difference of total flavonoid contents from the different tissues and varieties of Okra. Results indicated that the flavonoid content of the flowers was quite high compared to that of the root, stem, leaves, leaf stalk, and pod. Among the 13 different Okra varieties, the content of total flower flavoniods of Xiangkui No.3 was the highest. The content of total flavoniods of Xiangkui No.3 in pod was the highest among Xiangkui No.1, 3, 4, 5, 8. The above results provide a new way for the quality assessment and industrialization study of Okra.
	TO cite this article:Hu Shuai,Yuan Congying,LI Qiang, et al. Comparative study of total flavonoid contents from the different tissues and varieties of Abelmoschus Esculentus[OL].[22 November 2013] http://en.paper.edu.cn/en_releasepaper/content/4571299


	2. Overexpression, characterization, and dye-decolorizing ability of a thermostable, pH-stable, and organic solvent-tolerant laccase from Bacillus pumilus W3
	GUAN Zhengbing,SONG Chenmeng,ZHANG Ning,LIAO Xiangru
	Biology 24 October 2013
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	Abstract:Fungal laccases are typically unstable at high temperatures and alkaline conditions, thereby limiting their practical application. In this study, the novel laccase-producing Bacillus pumilus strain W3 was isolated from raw gallnut honey samples. The CotA-laccase gene was cloned from W3 and efficiently expressed by recombinant Escherichia coli in its biologically active form. The purified recombinant laccase had an extensive pH range for substrate catalysis. The enzyme was highly stable in alkaline pH and high temperatures, with considerable tolerance to NaCl and organic solvents. Laccase activity remained constant after 10 d of incubation at pH 9.0, whereas approximately 45% of the initial activity was detected after 10 h incubation at 80 C. Two azo dyes and two anthraquinonic dyes could be efficiently decolorized by purified laccase in the presence of a mediator under alkaline condition. More than 90% decolorization was observed at pH 9.0 after incubation for 5 h. These unusual properties indicated a high potential of the novel CotA-laccase for industrial application on decolorisation of textile dyeing effluent.
	TO cite this article:GUAN Zhengbing,SONG Chenmeng,ZHANG Ning, et al. Overexpression, characterization, and dye-decolorizing ability of a thermostable, pH-stable, and organic solvent-tolerant laccase from Bacillus pumilus W3[OL].[24 October 2013] http://en.paper.edu.cn/en_releasepaper/content/4565795

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	3. Cloning, Purification and Characterization of NADP+-dependent malic enzyme from the oleaginous fungus Mortierella alpina
	Jiayu Yang,Xinjie Hu,M\'balu.R.Kargbo,Haiqin Chen,Jianxin Zhao,Yuanda Song,Yong Q. Chen,Hao Zhang,Wei Chen
	Biology 12 March 2013
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	Abstract:Malic enzymes are a class of oxidative decarboxylases, that catalyze the oxidative decarboxylation of malate to pyruvate and carbon dioxide with concomitant reduction of NAD(P)+ to NAD(P)H. NADP+-dependent malic enzyme in oleaginous fungi plays a key role in fatty acid biosynthesis. In this work the malic enzyme-encoding cDNA(mce2) from oleaginous fungus Mortierella alpina was cloned and expressed in Escherichia coli BL21 (DE3). The recombinant protein (MaME) was purified by affinity chromatography using Ni-NTA Column. The purified enzyme used NADP+ as cofactor. Km value for L-malate and NADP+ were 2.19±0.01 mM and 0.38±0.02 mM respectively, while Vmax values were 147±2U/mg and 302±14U/mg respectively, at optimal condition of pH 7.5 and 33℃. MaME appeared to be activated by Mn2+, Mg2+, Co2+, Ni2+ and low concentration of Zn2+, but not by Ca2+, Cu2+ and high concentration of Zn2+. Oxaloacetic acid and glyoxylate inhibited the MaME activity by competing with malate and their Ki values were 0.08mM and 0.6mM respectively.
	TO cite this article:Jiayu Yang,Xinjie Hu,M\'balu.R.Kargbo, et al. Cloning, Purification and Characterization of NADP+-dependent malic enzyme from the oleaginous fungus Mortierella alpina[OL].[12 March 2013] http://en.paper.edu.cn/en_releasepaper/content/4529176


	4. Isolation and Characterization of Total N-linked Glycans from Glycoproteins by Ultrafiltration Units and Mass Spectrometry
	YANG Ganglong,MA Tianran,LI Zheng
	Biology 16 January 2013
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	Abstract:Glycosylation is one of the most common post-translational modifications in proteins. Current methods for glycan analysis are generally based on multiple preparation processes to separate glycans. However, glycans are continuously lost and the difficulty for accurate quantitative analysis is increased in the procedure. Here, a filter aided sample preparation-based total N-linked glycans from the glycoproteins isolation method (N-glycan-FASP-T) was developed using ultrafiltration units according to the molecular weight difference among the glycans, the impurities and proteins. Results: The isolated glycans were characterized and confirmed by the MALDI-TOF/TOF-MS. A total of 23 distinctive N-linked glycans were characterized from human sera.
	TO cite this article:YANG Ganglong,MA Tianran,LI Zheng. Isolation and Characterization of Total N-linked Glycans from Glycoproteins by Ultrafiltration Units and Mass Spectrometry[OL].[16 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4514464


	5. Preparation, Characterization and Application of Lectin-Magnetic Particle Conjugates
	YANG Ganglong,LI Zheng
	Biology 16 January 2013
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	Abstract:Magnetic particles have been developed for various applications in the biological and clinical determination. In this study, we synthesized the epoxy-activated magnetic particles to immobilization the lectins. The epoxy-activated magnetic particles were the superparamagnetic supports with a specific surface area of 96.29 m2/g. The each gram of the magnetic particles can be saturated with 186.31±5.16 mg Con A. The lectin-magnetic particle conjugates can isolate glycoproteins specially via the lectin's carbohydrate recognition domain with well reproducibility. This method can be a good tool to be applied in the glycoproteomics research.
	TO cite this article:YANG Ganglong,LI Zheng. Preparation, Characterization and Application of Lectin-Magnetic Particle Conjugates[OL].[16 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4514594


	6. Myosin light chain kinase inhibitor attenuates atherosclerosis and permeability via reduced endothelial tight junction in rabbits
	Zhu Huaqing,Zhou Qing,Wang Yuan
	Biology 27 December 2012
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	Abstract:The development of atherosclerosis is a multifactorial process in which elevated plasma cholesterol levels play a central role. This study analyzed the effects of myosin light chain kinase (MLCK) inhibitor (ML7) on endothelial permeability and gap formation and junction proteins expression in the artery wall of rabbits after feeding with a high-cholesterol diet. Further, we tested to determine whether that effects are involved in MLCK expression and MLC phosphorylation.We investigated the role of ML7 in the development of atherosclerotic lesions in New Zealand White rabbits fed an atherogenic diet for 12 weeks. Histopathological examination demonstrated that ML7 reduced the size of aortic lesions, lipid contents and endothelial permeability. Electron microscopy revealed ML7 attenuated gap formation in the endothelial tight junction. Immunohistochemistry indicated that ML7 decreased the expression of tight junction protein in the artery wall of rabbits fed with a high-cholesterol diet. Western blotting demonstrated that ML7 reduced occludin expression in the precipitation, but increased its expression in the supernatant of lysed aortas which indicated occludin occurred remodeling from cell membrane to cytoplasm. Further mechanistic studies showed ML7 attenuated endothelial barrier dysfunction through both MLCK expression and myosin light chain phosphorylation in aortic endothelial cells. We Concluded that ML7 contributes to atherosclerosis and endothelial barrier function by regulating tight junction proteins via mechanisms involving not only MLCK expression but also MLC phosphorylation.?????
	TO cite this article:Zhu Huaqing,Zhou Qing,Wang Yuan. Myosin light chain kinase inhibitor attenuates atherosclerosis and permeability via reduced endothelial tight junction in rabbits[OL].[27 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4508739


	7. Colorimetric detection of amplified target DNA by loop-mediated isothermal amplification using gold nanoparticle
	ZHOU Chao,MU Ying,JIN Wei,ZHANG Ying,JIN Qinhan
	Biology 13 December 2012
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	Abstract:We have developed a rapid, simple, and label-free colorimetric method for the identification of target DNA. It is based on loop-mediated isothermal amplification (LAMP). Plain gold nanoparticles (AuNPs) are used to indicate the occurrence of LAMP. Its products are mixed with AuNPs in an optimized ration, upon which the deoxyribonucleotides (dNTPs) bind to the AuNPs via ligand-metal interactions and thus enhance their stability. If a target DNA is amplified, the vast reduction of the dNTPs leads to the aggregation of AuNPs and a color change from red to blue. The success of the method strongly depends on the ionic strength of the solution and the initial concentration of dNTPs. Unlike other methods for identification of isothermal products, this method is simple and can be readily applied at sites where instrumentation is inadequate or even lacking.
	TO cite this article:ZHOU Chao,MU Ying,JIN Wei, et al. Colorimetric detection of amplified target DNA by loop-mediated isothermal amplification using gold nanoparticle[OL].[13 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4503864


	8. The effect of centrally administered apelin-13 on interdigestive gastric motility in conscious rats
	LV Shuangyu,QIN Yaojun,WANG Ningbo,YANG Yanjie,CHEN Qiang
	Biology 02 August 2012
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	Abstract:Apelin, a novel neoropeptide, was identified as the endogenous ligand for APJ receptor. The present study was designed to explore the effect apelin-13 on interdigestive gastric motility in conscious rats. Apelin-13 was synthesized by the solid-phase peptide synthesis method. After recovery from the operation of intracerebroventricular (i.c.v.) cannula implantation, the strain gauge transducers were implanted on the serosal surface of the antrum in Wistar rats. The migrating motor complex (MMC) was instantly recorded by the Power-Lab recording system. The results show that, comparing with control group (5.17±0.93), the frequency of the phase Ⅲ-like activity (c/h) of the 10 nmol apelin-13 group (7.67±1.33) and 30 nmol apelin-13 group (8.58±1.66) were both increased, showing a statistically significant difference (P < 0.01, P < 0.01, respectively). However, frequency of the phase Ⅲ-like activity of 3 nmol apelin-13 group (6.83±0.98) had no significant difference (P > 0.05). Comparing with control group (97.41±16.88), the %motor index (MI) of the 10 nmol apelin-13 group (143.93±26.27) and 30 nmol apelin-13 group (155.09±24.02) were both increased, indicating a statistically obvious difference (P < 0.01, P < 0.01, respectively). However, the %MI of 3 nmol apelin-13 group (120.14±3.00) showed no significant difference (P > 0.05). Our study first indicates that i.c.v. injection of apelin-13 increased the interdigestive antrum MMC in conscious rats, which has an important physiological significance for further study the modulatory effect of apelin-13 on the digestive tract motility.
	TO cite this article:LV Shuangyu,QIN Yaojun,WANG Ningbo, et al. The effect of centrally administered apelin-13 on interdigestive gastric motility in conscious rats[OL].[ 2 August 2012] http://en.paper.edu.cn/en_releasepaper/content/4485939


	9. Effects of Wingless / Wnt1 on the Development of Tribolium castaneum
	ZhuoyuLi,YananPeng,BinLi,ChengjunLi
	Biology 25 June 2012
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	Abstract:Known as highly conserved during evolution, the Wnt signaling pathway plays a vital role in regulating animal embryonic axis, embryonic differentiation, and deciding cell polarity and maintaining adult dynamic equilibrium. Mutations or deregulations of its components can cause the occurrence of carcinoma. We studied the role of Wingless/Wnt1 during larva-adult development of the red flour beetle, Tribolium castaneum, with dsRNA-mediated Wingless(Wg)/Wnt1 gene being knocked down. The treated late larvae metamorphosed into pupae with drastically increased wing interval and decreased wing width (P<0.01). The pursuant pupa-adult eclosion was also severely affected and most of pupae died during this period. The qPCR result showed that the mRNA level of Cadherin-like and Smoothened (Smo) genes were up-regulated greatly, and that of armadillo-2 was slightly higher , after Wingless/Wnt1 gene was knocked down. We drew the conclusion that Wnt-1 signaling pathway is closely related to the proper wing development and adult metamorphosis of Tribolium. In addition, the elevated expression of Cadherin-like and Armadillo-2 may be accountable for the reduced wing width and enlarged wing interval caused by wg gene silencing, because those alterations can either enhance cell adhesion or change cell morphology. Importantly, the up-regulation of smo gene indicates that Hedgehog signaling pathway may be affected by the RNAi of wg and involved in the abnormal or lethal phenotypes observed in our experiment.??
	TO cite this article:ZhuoyuLi,YananPeng,BinLi, et al. Effects of Wingless / Wnt1 on the Development of Tribolium castaneum[OL].[25 June 2012] http://en.paper.edu.cn/en_releasepaper/content/4482937


	10. Revealing a marine natural product as a novel agonist for retinoic acid receptors with a unique binding mode and inhibitory effects on cancer cells
	Wang Shanshan,Wang Zhao,Lin Shengchen,Zheng Weili,Wang Rui,Chen Jinan,Jin Lihua,Li Yong
	Biology 31 May 2012
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	Abstract:Retinoids display antitumor activity on various cancer cells and thereby have been used as important therapeutic agents. However, adverse side effects and retinoic acid (RA) resistance limit further development and clinical application of retinoid-based therapeutic agents. We report here the identification of a natural marine product that activates retinoic acid receptors (RARs) with a chemical structure distinct from retinoids by high-throughput compound library screening. Luffariellolide, was uncovered as a novel RAR agonist by inducing coactivator binding to these receptors in vitro, further inhibiting cell growth and regulating RAR target genes in various cancer cells. Structural and molecular studies unraveled a unique binding mode of this natural ligand to RARs with an unexpected covalent modification on the RAR receptor. Functional characterization further revealed that luffariellolide displays chemotherapeutic potentials for overcoming RA resistance in colon cancer cells, suggesting that luffariellolide may represent a unique template for designing novel non-retinoid compounds with advantages over current retinoic acid drugs.
	TO cite this article:Wang Shanshan,Wang Zhao,Lin Shengchen, et al. Revealing a marine natural product as a novel agonist for retinoic acid receptors with a unique binding mode and inhibitory effects on cancer cells[J].

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