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	1. The Protective Effect of ATP on Skeletal Muscle Satellite Cells Damaged by H2O2
	FEI Fei,ZHU Daoli,TAO Lijun,HUANG Baozhu,ZHANG Honghong
	Biology 21 April 2013
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	Abstract:Objective To investigate the protective effect of ATP on skeletal muscle satellite cells of newborn rats damaged by H2O2 and its influence on the related proteins Bax and Bcl-2. Methods The H2O2-induced damage models of skeletal muscle satellite cells of newborn rats were established and the study was divided into 4 groups：normal control group，model control group (0.1 mmol / L H2O2 solution damaged cells for 50s)，protection group(10，5，2.5，1.25，0.625，0.3125，0.15625 mg/mL ATP solutions treated cells and then 0.1 mmol / L H2O2 solution damaged cells for 50s)，proliferation group (10，5，2.5，1.25，0.625，0.3125，0.15625 mg/mL ATP solutions treated cells). MTT colorimetric analysis，FITC+PI+DAPI fluorescent staining，Giemsa staining and immunocytochemistry fluorescent apoptotic antibody test were applied to detect the survival rate of cells and the protective effect of ATP on skeletal muscle satellite cells damaged by H2O2. Results The survival rate of skeletal muscle satellite cells was decreased and the apoptosis rate was increased after damaged. The survival rate of muscle satellite cells protected by the ATP solution could be increased (P<0.01), and the apoptosis rate was decreased. Immunocytochemistry staining analysis results showed that ATP solution could enhance Bcl-2 proteins (P<0.01) and inhibite Bax proteins (P<0.01). Different doses of ATP solutions had different effects on skeletal muscle satellite cells induced by H2O2: the survival rate of muscle satellite cells protected by the adequate doses of ATP solutions （2.5、1.25、0.625 mg/mL）could be increased. The protective effect on cells treated by 1.25 mg/mL ATP solution was the most obvious. Cells treated by 10，5mg/mL ATP solutions could even cause obvious apoptosis. Conclusion The ATP solution had obvious protective effect on skeletal muscle satellite cells damaged by H2O2 in new-born rats. The main mechanism of ATP solution was related to strengthen the expression of Bcl-2 and inhibit that of Bax through JAK -STAT signal transduction, thus inhibiting apoptosis of cells.
	TO cite this article:FEI Fei,ZHU Daoli,TAO Lijun, et al. The Protective Effect of ATP on Skeletal Muscle Satellite Cells Damaged by H2O2[OL].[21 April 2013] http://en.paper.edu.cn/en_releasepaper/content/4539356


	2. Colon cancer cell supernatant polarized THP-1 macrophages to a M2 like phenotype
	Chen Nanpeng,Qi Caiwa,Chen Ying,Ding Wen,He Qingyu
	Biology 22 March 2011
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	Abstract:Tumor associated macrophages(TAMs) are tightly related to carcinogenesis, metastasis and invasion. It has been reported that the phenotype of tumor associated macrophages was rather different from the classical activated macrophages namely M1. TAMs produce anti-inflammtory and immune inhibitory cytokines and chemokines, thus inhibit the anti-tumor immunity within solid tumor. The mechanisms by which the tumor cell polarized macrophages to a pro-tumor state are largely unknown. In this study, we used flow cytometry and ELISA methods to analyze the effect of the condition medium from colon cancer cell line Caoco2 on the THP-1 macrophages. The results showed that the level of M2 markers, CD206 and CD163, were strongly upregulated, the M1 marker CCR7 was significantly downregulated. The cytokines IL-1b, TNF-a, IL-10 were all upregulated. Our research indicated that the tumor supernatant has the ability to induce macrophages to a M2 like phenotype.
	TO cite this article:Chen Nanpeng,Qi Caiwa,Chen Ying, et al. Colon cancer cell supernatant polarized THP-1 macrophages to a M2 like phenotype[OL].[22 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4417762

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