- Chinese︱Feedback︱Save this page
Get RSS feed
Check out RSS, or use RSS reader to subscribe this item 
Confirmation
Authentication email has already been sent, please check your email box: and activate it as soon as possible.
You can login to My Profile and manage your email alerts.
If you haven’t received the email, please:
- Login︱Register
|
|
|
 |
|
||
| There are 37 papers published in subject: > since this site started. | |||
| Select Subject |
| Select/Unselect all | For Selected Papers |
Saved Papers
Please enter a name for this paper to be shown in your personalized Saved Papers list
|
 |
| 1. Evidence of chilling-induced programmed cell death in cucumber fruit | |||
| Chen Xiaohong,Nie Peng,Mao Linchun | |||
Agronomy 20 December 2012
|
|||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:Cucumber fruit (Cucumis sativus L. cv. Zhexiu-1) were stored at 2℃ for 3 d, 6 d, 9 d and subsequently reconditioned at 20℃ for 2 d, respectively. Chilling injury (CI) was greatly aggravated upon reconditioning after chilling. Cell death occurred synchronously with CI acceleration. The hallmark of programmed cell death (PCD), DNA laddering, was clearly visualized by genome gel electrophoretic analysis upon reconditioning after chilling for 9 d with ethylene burst. Further evidence of nuclear DNA nicking was confirmed by the in situ TdT-mediated dUTP nick end labelling (TUNEL). In addition, chromatin condensation and nucleus distortion were observed by nuclear staining of DAPI. Results suggested that cell death arisen with the occurrence of chilling injury in cucumber fruit upon reconditioning after cold storage had the features of PCD process which may be mainly attributed to ethylene. | |||
| TO cite this article:Chen Xiaohong,Nie Peng,Mao Linchun. Evidence of chilling-induced programmed cell death in cucumber fruit[OL].[20 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4506670 | |||
| 2. Superoxide dismutase multigene family in longan somatic embryo: a comparison of the CuZn-SODs, Fe- SODs, and Mn-SOD gene structures,splicing, phylogeny, promoters and expression | |||
| Lin Yuling,Lai Zhongxiong | |||
Agronomy 04 December 2012
|
|||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract: Superoxide dismutases (SODs) encoded by a multigene family are important antioxidant enzymes that guard against superoxide toxicity. To date, no systematic characterization of this gene family has been conducted and their functions are not completely clear in plant embryos. 20 full-length cDNAs, encoding cytoplasmic CSD1a and DlCSD1b, chloroplast DlCSD2a and DlFSD1a, plastidic DlFSD1b and mitochondrial DlMSD, respectively, were obtained from longan embryogenic callus (EC) by RT-PCR and RACE. Each member contained multiple polyadenylation sites. The genomic structures of DlCSD1a, DlCSD1b, DlCSD2a, DlFSD1a, DlFSD1b and DlMSD genes consisted eight, six, seven, six, seven and five introns, respectively, and their introns lengths varied greatly. In addition, seven variants with different splicing modes were cloned, showing their functional diversity during longan somatic emrbyogenesis (SE). Further, each type of SOD gene had multiple transcription start sites (TSS), and the choice of TSS in SODs only affected the length of the 5'UTR, but not created protein diversity. Meanwhile, the promoters of DlCSD1a, DlCSD2a, DlFSD1a and DlMSD were isolated, which contained lots of cis-acting elements in response to light, GA, auxin, JA, MeJA, dehydration, coldness, wounding, etc. Comprehensive analysis of the expression profiles showed that the different types of SOD showed different spatial and temporal expression modes and played a key role especially at the middle and later developmental stages during longan SE. This study provided the comprehensive analysis of the whole SOD gene family from plants SE for the first time, including cloning of the full-length cDNAs, gene structures, alternatively spliced variants, phylogeny, TSS, isolation of promoters, and expression patterns, and these comparisons could provide a multifaceted view on diverse functions of the SOD isoforms during longan somatic embryos formation. | |||
| TO cite this article:Lin Yuling,Lai Zhongxiong. Superoxide dismutase multigene family in longan somatic embryo: a comparison of the CuZn-SODs, Fe- SODs, and Mn-SOD gene structures,splicing, phylogeny, promoters and expression[OL].[ 4 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4498654 | |||
| 3. Analyses of global transcriptomics and metabolic network on longan (Dimocarpus longan Lour.) embryogenic callus by Illumina paired-end sequencing | |||
| Lai Zhongxiong,Lin Yuling | |||
| Agronomy 05 February 2012
|
|||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:Background: Longan, a famous tropical/subtropical woody fruit in Southeast Asia. However, progress in the molecular mechanism of longan embryogenesis influencing yield and quality is slow by lack of transcriptomic and genomic information. Illumina second generation sequencing is suitable for generating enormous transcript sequences for functional genomic analysis for non-model species with un-sequenced genomes such as longan.Results: Longan EC(embryogenic callus) cDNA library was sequenced by Illumina HiSeq2000. The total of 64,876,258 clean reads with 5.84 Gb nucleotides were assembled into 68,925 unigenes with mean length of 448 bp, and ≥1000 bp unigenes accounted for 8.26 %. Based on BLASTx, 40,634 unigenes had significant similarity with Nr and Swiss-Prot databases. 38,845 and 17,118 annotated unigenes were respectively assigned to GO categories with 43 sub-categories and COG group with 25 sub-groups. Additionally, 17,306 unigenes were assigned to 199 KEGG pathways, which were well represented by Metabolic pathways, Plant-pathogen interaction, Biosynthesis of secondary metabolites, and Genetic information processing. Analyses of the unigenes (≥1000 bp) revealed that not only as many as 328 unigenes concerning embryogenesis-related genes such as EMB, PPR, MEE but also many unigenes concerning reproductive growth such as flowering, gametophyte genesis and fertility, and vegetative growth such as root and shoot growth, expressed in EC, which differed remarkably from the in vivo embryogenesis reported before. Conclusions: This research firstly provided a global transcriptome dataset of longan EC. The results showed that the expressed genes of EC were that much and so various, which suggested that EC almost reflect the whole profile of S.E., even seem to be the epitome of the whole plant on the molecular level. In a word, this dataset provided a new insight into molecular mechanism of embryogenesis in longan even in other plants. | |||
| TO cite this article:Lai Zhongxiong,Lin Yuling. Analyses of global transcriptomics and metabolic network on longan (Dimocarpus longan Lour.) embryogenic callus by Illumina paired-end sequencing[OL].[ 5 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4465149 | |||
| 4. Virus efficacy of recombined Autographa californica M nucleopolyhedrovirus (AcMNPV) on tea pest Ectropis obliqua | |||
| Liang Yuerong,Lin Chen,Wang Xunlei,Ye Jianhui,Lu Jianliang | |||
| Agronomy 25 January 2012 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:Ectropis obliqua is a major tea pest and chitin synthase (CHS) plays a key role in the pest growth and development. A 192 bp conserved domain from E. obliqua CHS gene was cloned and it was used to construct recombined Autographa californica M nucleopolyhedrovirus (AcMNPV) with double-stranded RNA interference (dsRNAi) method. The recombined AcMNPV virus could propagate in host cells sf9. Injection test showed that the virus efficacy of the recombined AcMNPV on E. obliqua larvae was significantly enhanced. It is considered that the CHS dsRNAi mediated by the Nuclear polyhedrosis virus will be interesting for development of alternative bio-pesticide to control the tea pest E. obliqua. | |||
| TO cite this article:Liang Yuerong,Lin Chen,Wang Xunlei, et al. Virus efficacy of recombined Autographa californica M nucleopolyhedrovirus (AcMNPV) on tea pest Ectropis obliqua[OL].[25 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4463882 | |||
| 5. Chitin synthase (CHS) gene in epidermis of Ectropis obliqua Prout | |||
| Liang Yuerong,Lin Chen,Wang Luoren,Ye Jianhui,Lu Jianliang | |||
| Agronomy 25 January 2012
|
|||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:Ectropis obliqua Prout is a major pest in tea fields and chitin synthase (CHS) plays an important role in biosynthesis of chitin and growth of the pest. A cDNA sequence encoding the CHS and its expression pattern during development of E. obliqua were investigated. The CHS cDNA sequence was 5496 bp nucleotides, with an open reading frame of 4692 bp encoding a protein of 1563 amino acids. It belonged to CHS-A member of CHS gene family. The CHS-A expression was the strongest in the third and fourth instar larvae, during which the growth rate of E. obliqua larvae was the rapidest. Catalysis model of CHS-A enzyme in E. obliqua was also hypothesized according to the specific motifs and topological structure prediction of the protein. This study provided an important information for the further research on development of RNA interference (RNAi) technology to control E. obliqua. | |||
| TO cite this article:Liang Yuerong,Lin Chen,Wang Luoren, et al. Chitin synthase (CHS) gene in epidermis of Ectropis obliqua Prout[OL].[25 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4463876 | |||
| 6. FLOWER BUD DIFFERENTIATION PATTERNS OF THREE LILY CULTIVARS | |||
| ZHANG Yingjie,CHEN Xiaofang,WU Shasha,LIU Xiaohua,CHEN Lina,WU Jindi,JIAO Xuehui,LI Ranxin,WANG Shuang,LU Yingmin | |||
| Agronomy 31 December 2011
|
|||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:To study the flower bud differentiation process of lilies, Asiatic hybrids ‘Pollyanna’, ‘Prato’and Oriental hybrid ‘Siberia’ were observed under microscope. The study indicates that ‘Pollyanna’ and ‘Siberia’ have the same pattern in the course of flower bud differentiation. They translated from vegetative growth to reproductive growth neither after harvest, nor during the cold storage period, but only happened after being planted. ‘Prato’ started bud differentiation when they were harvest, and had further changes during the cold treatment. And the bud differentiation was finished after planting. Moreover, the study indicates that except the growing point, starch contents in different parts of lily bulbs descended during the cold treatment, and increased after the low appeared. Starch contents in outer scales and inner scales had the same trend with the starch content in inner scales changed more remarkably. On the other hand, changes of sucrose contents in these parts had the opposite trend compared with the changes of starch contents. | |||
| TO cite this article:ZHANG Yingjie,CHEN Xiaofang,WU Shasha, et al. FLOWER BUD DIFFERENTIATION PATTERNS OF THREE LILY CULTIVARS[OL].[31 December 2011] http://en.paper.edu.cn/en_releasepaper/content/4458921 | |||
| 7. The Effects of Exogenous H2O2 and AsA on Occurrence of Hyperhydricity and Production and Localization of Endogenous ROS in Garlic Plantlet in Vitro | |||
| JIANG Fangling,JIN Huiqing,TIAN Jie,CAO Xue,LIU Min,LI Jieying,WU Zhen | |||
Agronomy 30 December 2011
|
|||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:In order to further confirm the connection between reactive oxygen species(ROS) and hyperhydricity, in this experiment, using garlic variety 'Ershuizao' as test material, the changes of hyperhydricity rate and the generation and localization of endogenous ROS of garlic plantlet in vitro were studied under exogenous hydrogen peroxide(H2O2), exogenous H2O2 + Ascorbic acid(AsA, a ROS scavenger) treatments with plantlet under normal culture as the control. The results showed that the hyperhydricity rate under exogenous H2O2 treatment was 30.66% on day 16 after treatment, significantly higher than that of the control(13.37%) and exogenous H2O2+AsA treatment(19.22%). Compared with the control, the O2.- generation rate and H2O2 level of garlic shoots treated by exogenous H2O2 on the 8th day increased significantly; whereas exogenous H2O2 + AsA treatment reduced the generation of O2.- and H2O2 significantly, accordingly, hyperhydricity rates decreased significantly. The histochemical localization of O2.- and H2O2 demonstrated that endogenous ROS accumulated mainly in leaves and pesudostems under different treatments with the H2O2 treatment exhibited the largest accumulation. Cytochemical staining showed that H2O2 accumulation was observed mostly in cell wall, intercellular space and chloroplast. The studies preliminarily confirmed that exogenous H2O2 stress enhanced the generation of endogenous O2.- , H2O2 and the occurrence of hyperhydricity of garlic plantlet in vitro effectively, and application of exogenous AsA significantly decreased the accumulation of ROS and hyperhydricity rate induced by H2O2 stress, indicating a close connection between ROS and hyperhydricity. | |||
| TO cite this article:JIANG Fangling,JIN Huiqing,TIAN Jie, et al. The Effects of Exogenous H2O2 and AsA on Occurrence of Hyperhydricity and Production and Localization of Endogenous ROS in Garlic Plantlet in Vitro[OL].[30 December 2011] http://en.paper.edu.cn/en_releasepaper/content/4457586 | |||
| 8. Maturity-dependent chilling tolerance is regulated by redox state in cucumber (Cucumis sativus L.) fruit | |||
| QIAN Chunlu,MAO Linchun | |||
| Agronomy 04 November 2011
|
|||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:This study investigated antioxidative response to chilling stress in cucumber fruit at defined developmental stages. Chinese mini-cucumber (cv. Hangcui-1) was grown under commercial practice. Cucumber fruit were harvested at four developmental stages: Immature (3-8 DAA), Mature (9-16 DAA), Breaker (17-22 DAA), and Yellow (35-40 DAA). Fruit were stored at 2℃ for 9 days and rewarmed at 20℃ for 2 days. Visual observation revealed that sensitivity to chilling stress was highly maturity-dependent and fruit in earlier development stage were more susceptible to chilling injury. Increased oxidative stress was observed by increased superoxide anion radical production rate and hydrogen peroxide content under cold storage and rewarming. The data also confirmed that high superoxide dismutase (SOD), peroxidase (POD), ascorbate (ASC) content and ASC/DHA redox state seems to be critical for chilling tolerance. | |||
| TO cite this article:QIAN Chunlu,MAO Linchun. Maturity-dependent chilling tolerance is regulated by redox state in cucumber (Cucumis sativus L.) fruit[OL].[ 4 November 2011] http://en.paper.edu.cn/en_releasepaper/content/4448538 | |||
| 9. Selection of reliable reference genes for expression studies by reverse transcription quantitative real-time PCR in litchi under different experimental conditions | |||
| ZHONG Haiying,CHEN Jianwen,LI Caiqin,WU Jianyang,CHEN Lei,CHEN Jianye,LU Wangjin,LI Jianguo | |||
| Agronomy 21 March 2011
|
|||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:Reverse transcription quantitative real-time PCR (RT-qPCR), a sensitive technique for quantifying gene expression, depends on the stability of the reference gene(s) used for data normalization. Only a few studies on reference genes have been done in fruit trees and none in litchi. In the present work, seven frequently used candidate reference genes, including actin (ACTIN), glyceraldehyde-3-phosphate-dehydrogenase (GADPH), elongation factor 1-alpha (EF-1α), poly ubiquitin enzyme (UBQ), α-tubulin (TUA), β-tubulin (TUB) and RNA polymerase II transcription factor (RPII) were evaluated for their expression stability in litchi. Seventy-eight samples, including different varieties, tissues, organs, developmental stages and treatments, such as NAA, shading, and girdling plus defoliation, were addressed in this analysis. Our results showed that GAPDH was the most suitable reference gene among all the tested samples, different organs and NAA treatment. ACTIN was stably expressed in varieties and fruit developmental stages. RPII and UBQ exhibited the better expression stability in tissues. EF-1α was the most stable gene in shading and girdling plus defoliation treatments. Moreover, using combination of two genes as reference genes might improve the reliability of gene expression by RT-qPCR in litchi. The better combination was GAPDH + EF-1α or GAPDH + ACTIN for all the examined samples. In addition, the validated reference genes were further relied when used to quantify the expression of an interested gene, LcARF13 under different experimental conditions. These results firstly provide guidelines for reference genes selection under different experimental conditions and a foundation for more accurate and widespread use of RT-qPCR in litchi. | |||
| TO cite this article:ZHONG Haiying,CHEN Jianwen,LI Caiqin, et al. Selection of reliable reference genes for expression studies by reverse transcription quantitative real-time PCR in litchi under different experimental conditions[OL].[21 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4417495 | |||
| 10. Light-induced gene expression involved in phenylpropanoid biosynthetic pathways in callus of tea (Camellia sinensis L.) | |||
| Wang YunSheng ,Gao LiPing ,Wang ZhengRong ,Liu YaJun ,Sun MeiLian ,Yang DongQing ,Wei Chaoling ,Shan Yu ,Luo Yang ,Xia Tao | |||
| Agronomy 11 March 2010 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:Tea (Camellia sinensis L.) is a commercially important crop that is valued for its secondary metabolites. Light can influence the accumulation of phenylpropanoids (including catechins, anthocyanins, and lignins) in the seedlings of tea plants. To investigate the molecular mechanism by which light influences phenylpropanoid metabolism, we established light-induced suppression subtractive hybridization (SSH) cDNA libraries of tea callus. A total of 191 clones from the forward (light-induced) SSH library and 74 clones from the reverse (darkness control) library were selected, sequenced, and analyzed in this study. BlastX analysis of the tea callus light-induced cDNA library showed that the subtractive expressed sequence tags (ESTs) were classified into 12 putative cellular functions, in which the ‘photomorphogenesis’, ‘stress defense’, ‘signal transduction’, and ‘phenylpropanoid and secondary metabolism’ categories contained 5, 17, 13, and 9 ESTs, respectively. The bioinformatic sequence analysis of the SSH library, associated with morphological comparison, secondary metabolites analysis, and representative genes expression analysis, implied that: (1) light could inhibit the growth, and promote the differentiation, of the tea callus; (2) light might be effective for activation of the biosynthesis of phenylpropanoids, e.g. analysis and identification of catechins implied that light irradiation could activate epicatechin and epigallocatechin biosynthesis; and (3) light, as a signal or a stress condition, may regulate photomorphogenesis and phenylpropanoid synthesis mediated by the cAMP and Ca2+ signal pathway in tea callus. The SSH library established in this study represents a valuable resource for future research aimed at improving economically important secondary metabolic characteristics in the tea plant. | |||
| TO cite this article:Wang YunSheng ,Gao LiPing ,Wang ZhengRong , et al. Light-induced gene expression involved in phenylpropanoid biosynthetic pathways in callus of tea (Camellia sinensis L.)[OL].[11 March 2010] http://en.paper.edu.cn/en_releasepaper/content/40595 | |||
| Select/Unselect all | For Selected Papers |
Saved Papers
Please enter a name for this paper to be shown in your personalized Saved Papers list
|
|
 |
|
||||||||||||||
About Sciencepaper Online |
Privacy Policy |
Terms & Conditions |
Contact Us
Technology Partner - CERNET