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	1. Sequence analysis of a plasmid from wheat blue dwarf phytoplasma
	CHEN Wang,LI Yan,LIU Shubin,WU Yunfeng
	Agronomy 17 December 2013
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	Abstract:A plasmid of wheat blue dwarf (WBD) phytoplasma, named pWBDa, were cloned. It was 3449bp in length. Sequence analysis revealed that four proteins were encoded by this plasmid, including two secreted protein and four membrane proteins. Replication-associated protein (RepA), cop number control protein (Cop) and single-stranded DNA-binding protein (SSB) were found in these four proteins.
	TO cite this article:CHEN Wang,LI Yan,LIU Shubin, et al. Sequence analysis of a plasmid from wheat blue dwarf phytoplasma[OL].[17 December 2013] http://en.paper.edu.cn/en_releasepaper/content/4576092


	2. Sorption, desorption and mobility characteristics of a novel fungicide pyrazoxystrobin in three agricultural soils
	Liu Xunyue,Ding Xingcheng
	Agronomy 20 January 2012
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	Abstract:Pyrazoxystrobin, (E)-methyl 2-(3-((3-(4-chlorophenyl)-1-methyl- 1H -pyrazol-5-yloxy) methyl) phenyl) -3 - methoxyacrylate, is a novel fungicide developed in China. The adsorption, desorption and mobility characteristics of pyrazoxystrobin on three agricultural soils were studied by using 14C labeled compound. The adsorption of pyrazoxystrobin in soils was correlated to soil organic matter content and soil pH and the sorption has been observed in the order of S2>S1>>S3. Desorption was reflect some of the interactions involved between the pesticides and the soil components, soil pH seems to more influence to pyrazoxystrobin desorption and only about 2.54 to 6.41% of adsorbed pyrazoxystrobin was released. The mobility result, minimum of 95.02% compound residued in the upper 4.0 cm layer, demonstrated the use of pyrazoxystrobin as a fungicide is likely to be safe to groundwater.
	TO cite this article:Liu Xunyue,Ding Xingcheng. Sorption, desorption and mobility characteristics of a novel fungicide pyrazoxystrobin in three agricultural soils[OL].[20 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4463029


	3. Effect of Cryptococcus laurentii and calcium chloride on reduction of Penicillium expansum and Botrytis cinerea infections in pear fruit
	YU Ting,LU Huangping,ZHENG Xiaodong
	Agronomy 26 October 2011
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	Abstract:This study was conducted to evaluate the efficacy of the biocontrol yeast Cryptococcus laurentii and calcium chloride (CaCl2) in suppressing the blue and gray mold rots of pear and to explore possible mode of action involved. The results showed that the combined treatment of pear fruit wounds with C. laurentii and CaCl2 at 2% (w/v) resulted in a significantly improved inhibition of Penicillium expansum and Botrytis cinerea infections in comparison with the application of C. laurentii alone. CaCl2 neither affected the growth of C. laurentii in vitro or in vivo, nor directly inhibited the blue mold in pear fruit wounds, however, it induced the fruit resistance to the blue mold rot when the time interval between CaCl2-treatment and pathogen-inoculation was up to 24 h, being associated with an activation of the peroxidase activity of pear fruit. Therefore, it could be concluded that the mechanism by which CaCl2 reinforced the biocontrol efficacy of C. laurentii against the mold infections was mainly due to its ability to inducing the fruit natural resistance.
	TO cite this article:YU Ting,LU Huangping,ZHENG Xiaodong. Effect of Cryptococcus laurentii and calcium chloride on reduction of Penicillium expansum and Botrytis cinerea infections in pear fruit[OL].[26 October 2011] http://en.paper.edu.cn/en_releasepaper/content/4447456


	4. Prophenoloxidase-1 (PPO1) cDNA clone and its expression in Diamondback moth Plutella xylostella
	Xue Chaobin,Luo Wanchun
	Agronomy 10 December 2010
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	Abstract:Phenoloxidase (PO) is a key enzyme in insect development, responsible for catalyzing the hydroxylation of tyrosine into o-diphenols and the oxidation of o-diphenols into o-quinones. In this paper, the prophenoloxidase-1 (PxPPO1) cDNA of Plutella xylostella was cloned by means of reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The full-length cDNA consisted of 2838bp, containing an open reading frame (ORF) of 2049bp which encoding 682 amino acids. The molecular mass of the deduced amino acids and the active enzyme was predicted to be 78.56kDa and 72.65 kDa, respectively. The calculated pI was 6.43. BLASTp search and neighbor-joining analysis showed that the deduced amino acid sequence had a high identity to the published sequence of PPO1 from other lepidopterous insects, ranging from 70.5% to 74.6%. Protein signature analysis revealed three conserved regions, including the two copper binding sites characteristic of arthropod PPOs. Semi-quantitative RT-PCR and Real-time PCR indicated that the highest amount of PxPPO1 transcripts in eggs or the 4th instar larvae, followed by the 2nd, the 3rd instar larvae, prepupae and pupa.
	TO cite this article:Xue Chaobin,Luo Wanchun. Prophenoloxidase-1 (PPO1) cDNA clone and its expression in Diamondback moth Plutella xylostella[OL].[10 December 2010] http://en.paper.edu.cn/en_releasepaper/content/4396723


	5. Design, Synthesis and Bioactivities Screening Based on Ca2+-Mg2+-ATPase and AChE Target of A Diaryl Keones-Inspired Pesticide Molecules Library
	Jin Hong ,Tao Ke ,Hou Taiping
	Agronomy 04 February 2009
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	Abstract:Chemical biology plays significant role in modern drug design and pesticide discovery. Bio-target based structural optimization may speed up the new drug development. A collection of bio-active compounds is the prerequisite for pesticide discovery. We herein described the development of a collection of compounds based on the diaryl ketone shared by three natural products, 1,5-diphenylpentan-1-one, 1,5-diphenylpent-2-en-1-one and 3-hydroxy-1,5-diphenylpentan-1-one with good insecticidal activities from extracts of Stellera chamaejasme L. of Qinghai-Tibet Plateau in China. Based on their shared diaryl ketone moiety as ‘pharmacophore’, a series of diaryl ketones were synthesized and subjected for insecticidal and Acetylcholinesterase (AChE) inhibitory activities examination. All synthesized compounds showed poor insecticidal and AChE inhibitory activity. However, some compounds showed strong activities against plant pathogenic fungi suggesting their potential usage in fungi prevention. Present study provides basis for further pesticide discovery.
	TO cite this article:Jin Hong ,Tao Ke ,Hou Taiping . Design, Synthesis and Bioactivities Screening Based on Ca2+-Mg2+-ATPase and AChE Target of A Diaryl Keones-Inspired Pesticide Molecules Library[OL].[ 4 February 2009] http://en.paper.edu.cn/en_releasepaper/content/28392

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