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	1. A novel strategy to enhance resistance to Cucumber mosaic virus in tomato by grafting to transgenic rootstocks
	BAI Miao,CHEN Wenting,XIE Bingyan,YANG Guoshun
	Agronomy 11 May 2016
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	Abstract:Cucumber mosaic virus (CMV) can infect a wide range of host species. For the lacking of CMV-resistant varieties of tomato, RNA interference can be used as a fast and effective method for the generation of transgenic resistant varieties. In this current study, five intron-spliced hairpin RNA (ihpRNA) plant expression vectors aim at five genes of CMV has been constructed. Transgenic tomatoes were obtained by Agrobacterium mediated transformation with expression vectors. Highly resistant generations of transgenic plants were employed as rootstocks and grafted onto non-transgenic tomatoes that resulted in the successful transfer of resistance to the scions. Using a novel method of plant cuttings for rootstock propagation, we obtained large quantities of disease-resistant material. Further, this method produces scions that can remain undetectable for transgenic resistance marker genes that may provide novel approaches to evade collective concerns about genetically-modified organism (GMO) biosafety.
	TO cite this article:BAI Miao,CHEN Wenting,XIE Bingyan, et al. A novel strategy to enhance resistance to Cucumber mosaic virus in tomato by grafting to transgenic rootstocks[OL].[11 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4687477


	2. Temporary inhibition of station-keeping response to light in migratory rice planthoppers
	Hai-Bo YANG,Gao HU,Fan FANG,Baoping ZHAI
	Agronomy 07 March 2015
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	Abstract:This study aims to determine whether or not migratory rice planthoppers [Sogatella furcifera (Horváth) and Nilaparvata lugens] temporarily inhibit their station-keeping response to light during migration and to elucidate whether or not these insects respond to light traps located in their native habitat. Results of mark-release-recapture experiments revealed that the overall recapture rate was very low (0.028%) and that almost all emigrating macropterous adults did not respond to local light traps. The population dynamics of planthoppers under light traps were not synchronous with that in paddy fields. The peaks of planthoppers caught by light traps appeared when the low-level wind jets at 850 hPa isobaric surfaces, and these catches were almost immigrants from distant places. The macropterous planthopper adults in the field can be passively dispersed when their take-off was suppressed by precipitation and then would respond to light traps at night. These results show that migrating planthoppers are not distracted by light traps in their native habitat. This study provided interpretation for the definition of migratory behaviour.
	TO cite this article:Hai-Bo YANG,Gao HU,Fan FANG, et al. Temporary inhibition of station-keeping response to light in migratory rice planthoppers[OL].[ 7 March 2015] http://en.paper.edu.cn/en_releasepaper/content/4633549


	3. Revision of a common erythroneurine pest occurring on peach trees in China (Hemiptera: Cicadellidae: Typhlocybinae)
	CAO Yanghui,ZHANG Yalin
	Agronomy 22 July 2013
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	Abstract:A common leafhopper pest on peach trees, Táoyīdiǎnyèchán (桃一点叶蝉), has previously been misidentified as Erythroneura sudra (Distant, 1908). The correct identification is here shown to be Singapora shinshana (Matsumura, 1932) based on available descriptions, illustrations and records of distribution and host plants. Habitus photos and illustrations of the male genitalia for both species are provided for comparison.
	TO cite this article:CAO Yanghui,ZHANG Yalin. Revision of a common erythroneurine pest occurring on peach trees in China (Hemiptera: Cicadellidae: Typhlocybinae)[OL].[22 July 2013] http://en.paper.edu.cn/en_releasepaper/content/4552933


	4. The complete mitochondrial genome of Gynaephora menyuanensis (Lepidoptera: Lymantriidae) from the Qinghai-Tibetan Plateau
	YUAN Minglong,ZHANG Qilin
	Agronomy 24 February 2013
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	Abstract:We sequenced and annotated the complete mitochondrial genome (mitogenome) of Gynaephora menyuanensis (Lepidoptera: Lymantriidae), which is a major pest in alpine meadows and endemic to the Qinghai-Tibetan Plateau. This mitogenome is 15,770 bp in length with an A+T content of 81.48%, and contains 37 typical animal mitochondrial genes that are arranged in the same order as that of other lepidopteran species. All protein-coding genes (PCGs) start with a typical ATN codon, with the exception of cox1, which begins with CGA. Eleven PCGs stop with termination codon TAA, whereas cox2 and nad4 have single T as the incomplete stop codon. All of the 22 transfer RNA genes present the typical clover leaf secondary structure. The A+T-rich region is located between rrnS and trnM with a length of 449 bp, and contains a 19 bp poly-T stretch as found in other lepidopteran mitogenomes. This is the second completely sequenced mitogenome from the family Lymantriidae of Lepidoptera, providing important molecular information to phylogenetic and population genetic researches.
	TO cite this article:YUAN Minglong,ZHANG Qilin. The complete mitochondrial genome of Gynaephora menyuanensis (Lepidoptera: Lymantriidae) from the Qinghai-Tibetan Plateau[J].


	5. Comparative population genetics of Liposcelis bostrychophila and L. entomophila, from China inferred by analysis of the mitochondrial Cytb Gene
	WEI Dandan,WU Jingjing,DOU Wei,WANG Jinjun
	Agronomy 10 January 2012
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	Abstract:The psocids Liposcelis bostrychophila and L. entomophila (Psocoptera: Liposcelididae), are two major pests of stored grain and commonly occur on a wide range of stored products. Increasingly, psocids have gained recognition of their importance due to their feeding on stored grains, contaminating food and agricultural commodities. Psocids are difficult to control and exhibit high levels of resistance to various insecticides. Previous research has mainly focused on psocid physiology, biochemistry, and basic biology in grain storage systems. However, the population genetic structure has not been well categorized, which may be useful for understanding the distribution of resistance gene frequencies among psocid populations, gene flow patterns, dispersal of source populations, and the resultant genetic structure among populations. We generated mtDNA sequence data for 148 L. entomophila individuals from ten collecting locations and 103 L. bostrychophila individuals from eight locations in China. Population genetic analyses indicated that the obligatory parthenogenetic L. bostrychophila had high genetic diversity, and is higher than the genetic diversity of sexually reproducing psocid L. entomophila. Both species had a significant genetic structure and significant genetic differentiation existed amongst the populations. The Mantel test indicated that there was no evidence for isolation-by-distance for both species. The neutrality test revealed that the two species might have undergone population expansions in the past in the sampled areas. In addition, population differentiation of both psocids may mainly due to other factors such as genetic drift or inbreeding and less by geographic distance.
	TO cite this article:WEI Dandan,WU Jingjing,DOU Wei, et al. Comparative population genetics of Liposcelis bostrychophila and L. entomophila, from China inferred by analysis of the mitochondrial Cytb Gene[OL].[10 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4461243


	6. Identification of HrpX Regulon Genes in Xanthomonas oryzae pv. oryzicola using a GFP visualization technique
	LI Yurong,XIAO Youlun,ZOU Lifang,ZOU Huasong,CHEN Gongyou
	Agronomy 02 November 2011
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	Abstract:Xanthomonas oryzae pv. oryzicola is the causal agent of bacterial leaf streak in rice and injects repertoires of T3S effectors (T3SEs), which are normally regulated by a global regulator HrpX, into plant cells to suppress plant innate immunity for disease development. To establish a visualization technique to identify HrpX regulon genes in this pathogen, we chose six known or unknown T3SE genes of X. oryzae pv. oryzicola (strain RS105) as the targets in this report. The promoters of these candidates, whether or not containing a PIP-box (plant-inducible promoter), were fused with the gfp (green fluorescent protein) reporter gene, and the gfp reporters were introduced into the wild-type RS105 and the hrpX mutant RΔhrpX. GFP expression in the wild-type strain could be observed under fluorescence microscopy, but fluorescence was not observed in the hrpX mutant. Consequently, transcription and secretion detection demonstrated that these HrpX regulon members were repressed when hrpX was mutated and were not secreted into the hrp-inducing medium XOM3 because of a mutation in hrcV (T3SS deficient). These data suggest that the gfp reporter system is a feasible visualization tool for identification of HrpX regulon genes and discovery of new T3SEs in the X. oryzae pv. oryzicola-rice pathosystem.
	TO cite this article:LI Yurong,XIAO Youlun,ZOU Lifang, et al. Identification of HrpX Regulon Genes in Xanthomonas oryzae pv. oryzicola using a GFP visualization technique[OL].[ 2 November 2011] http://en.paper.edu.cn/en_releasepaper/content/4447535


	7. Effect of vegetation of transgenic Bt rice and its straw amendment on soil enzymes, respiration and functional diversity of soil microorganisms under field conditions
	Dong Bin,Yan Hu,Yu Yunlong
	Agronomy 17 December 2010
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	Abstract:With the development of transgenic crops, there is an increasing concern about the possible adverse effects of their vegetation and residues on soil environmental quality. To evaluate the effect of vegetation of transgenic Bt rice followed by return of rice straw on microbe-mediated functions in soil under field conditions, two Bt transgenic rice lines, Huachi B6 (Bt-HC) and TT51 (Bt-T), were adopted to assess the possible effect on soil enzyme (neutral phosphatase, urease, catalase, and invertase) activities, anaerobic respiration activity, and microbial utilization of carbon substrates. The results indicated that the vegetation of the two transgenic rice lines, Huachi B6 (Bt-HC) and TT51 (Bt-T), and addition of their straw have few adverse effect on soil enzymes and anaerobic respiration activity, compared to their parent and distant parent, although some transient or even significant differences were observed. The vegetation and straw amendment of Bt rice B6 (Bt-HC) and TT51 (Bt-T) did not appear to have harmful effect on the richness and evenness of soil microorganisms. The dominant population of soil microorganisms seemed to be more sensitive and altered temporarily by vegetation and straw amendment of Bt rice. This alteration is, however, smaller than that caused by vegetation and straw amendment of non-transgenic distant parental rice. No different pattern of impact duo to plant species was found between B6 (Bt-HC) and TT51 (Bt-T). It could be concluded from the results obtained in this study that vegetation of Bt rice and the return of its straw as fertilizer will not alter soil microbe-mediated functions.
	TO cite this article:Dong Bin,Yan Hu,Yu Yunlong. Effect of vegetation of transgenic Bt rice and its straw amendment on soil enzymes, respiration and functional diversity of soil microorganisms under field conditions[OL].[17 December 2010] http://en.paper.edu.cn/en_releasepaper/content/4398651

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