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	1. The genus Cratospila Foerster (Hymenoptera: Braconidae: Alysiinae) in China, with two newly recorded species
	Yao Junli,Cornelis van Achterberg,Chen Jiahua
	Agronomy 30 May 2016
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	Abstract:Cratospila F?rster is a small genus in Alysiini (Hymenoptera: Braconidae: Alysiinae). Two species of the genus Cratospila Foerster (C. tricolor Telenga and C. neocirce Wharton) are newly reported from China and a key to the Chinese species is given. The Nearctic C. neocirce Wharton 1980, is reported for the first time from the Palaearctic Region.
	TO cite this article:Yao Junli,Cornelis van Achterberg,Chen Jiahua. The genus Cratospila Foerster (Hymenoptera: Braconidae: Alysiinae) in China, with two newly recorded species[OL].[30 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4692915


	2. Characterization and function of Tomato yellow leaf curl virus-derived small RNAs generated in tolerant and susceptible tomato varieties
	BAI Miao,YANG Guoshun,LIN Runmao,CHEN Wenting,LING Jian,Mao Zhenchuan,XIE Bingyan
	Agronomy 11 May 2016
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	Abstract:Virus-tolerant plant, which allows the accumulation of virus and then generate virus-derived small RNAs (vsRNAs), valuable materials to reveal the antiviral efficiency of vsRNAs. Here, a comparison of vsRNAs in Tomato yellow leaf curl virus tolerant and in susceptible tomato varieties showed the consistent trend of vsRNAs' distribution on virus genome, which is presented as an obvious characteristic. However, the expression level of vsRNA in tolerant variety is less than that in susceptible variety. Slicing targets of vsRNA-mediated viral transcripts were investigated using parallel analysis of RNA ends, and geminivirus DNA methylation was determined by bisulfite sequencing, which uncovered that not all vsRNAs participated in viral mRNA degradation and DNA methylation. Additionally, by comparing with the expression pattern of vsRNAs, viral DNA and mRNA, we proposed the quantity of vsRNAs is corresponding to the expression level of viral mRNA, while the virus-suppression of vsRNAs is not high-efficient.
	TO cite this article:BAI Miao,YANG Guoshun,LIN Runmao, et al. Characterization and function of Tomato yellow leaf curl virus-derived small RNAs generated in tolerant and susceptible tomato varieties[OL].[11 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4687558

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	3. Study of PxPPO1 expression in Plutella xylostella
	LU Yanli,LI Bin,JIN Minghui,XUE Chaobin
	Agronomy 19 December 2013
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	Abstract:Insect prophenoloxidase (PPO) is a copper-containing enzyme, playing an important role in immunity reaction. In this paper, the expression of PxPPO1 at different life stages were investigated, Semi-quantitative PCR and Real-time quantitative PCR indicated that the highest amount of PxPPO1 transcripts in eggs and the 4th instar larvae, followed by the 2nd, the 3rd instar larvae, prepupae and pupa. The ORF of PxPPO1 was cloned and inserted into pET-30a prokaryotic expression vector, the recombinant proteins was expressed in Escherichia coli, and was confirmed with an 83 kDa visible, but insoluble protein.
	TO cite this article:LU Yanli,LI Bin,JIN Minghui, et al. Study of PxPPO1 expression in Plutella xylostella[OL].[19 December 2013] http://en.paper.edu.cn/en_releasepaper/content/4575162


	4. Enzymatic properties of phenoloxidase from Anomala corpulenta Larvae
	CUI Jingjing,LI Bin,LIU Wei,XUE Chaobin
	Agronomy 14 December 2013
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	Abstract:The kinetic parameters of partially purified phenoloxidase (PO, EC. 1.14.18.1) from the larvae of Anomala corpulenta M. were determined, using L-3, 4-dihydroxyphenylalanine (L-DOPA) as substrate. The optimal pH and temperature of the enzyme for the oxidation of L-DOPA were determined to be at pH 7.5 and at 50 C, respectively. At pH 7.5 and 50 C, the Michaelis constant (Km) and maximal velocity (Vm) of the enzyme for the oxidation of L-DOPA were determined to be 11.11 mmol/L and 107.53 U/mg, respectively, and the Km and Vm of PO for the oxidation of catechol were 9.52 mmol/L and 41.67 U/mg, respectively. Morin could effectively inhibit the activity of phenoloxidase and this inhibition was reversible and competitive, with the IC50 of 1.05 mmol/L. The inhibition constants were estimated to be 0.54 mmol/L.
	TO cite this article:CUI Jingjing,LI Bin,LIU Wei, et al. Enzymatic properties of phenoloxidase from Anomala corpulenta Larvae[OL].[14 December 2013] http://en.paper.edu.cn/en_releasepaper/content/4575150


	5. Biosuppression of fungal decay in pear fruit by chitosan integration with biocontrol yeast and calcium chloride
	YU Ting,LU Huangping,ZHENG Xiaodong
	Agronomy 02 November 2011
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	Abstract:This study evaluated the performance of chitosan at different concentrations alone, and in combination with a biocontrol yeast Cryptococcus laurentii and calcium chloride in control of the blue mold decay in pear fruit. The results showed that efficacy of chitosan in inhibiting blue mold decay in pear fruit decreased as the incubation time increased. Chitosan at 0.5% or above delayed the rapid growth of C. laurentii, nevertheless, it enhanced remarkably mold decay control when in combination with C. laurentii. Moreover, addition of calcium chloride the mixture of C. laurentii and chitosan further enhanced the decay control, although calcium chloride had little antifungal activity. Therefore, a combination of chitosan with C. laurentii and calcium chloride provides a great new potential for development of effective alternative approach to inhibit the blue mold decay especially in an integrated postharvest diseases management strategy.
	TO cite this article:YU Ting,LU Huangping,ZHENG Xiaodong. Biosuppression of fungal decay in pear fruit by chitosan integration with biocontrol yeast and calcium chloride[OL].[ 2 November 2011] http://en.paper.edu.cn/en_releasepaper/content/4448268


	6. Molecular analysis of quizalofop-resistant Echinochloa crusgalli reveals a Leu-1781-Ile mutation
	HUAN Zhibo,WANG Jinxin
	Agronomy 06 March 2011
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	Abstract:A resistant Echinochloa crusgalli (barnyard grass) population which survived after repeated application of quizalofop in China was collected. After self-pollination and outcross, a homozygous germplasm (RR) was obtained. The resistant germplasm (RR) was found highly resistant to quizalofop compared with the sensitive germplasm (SS) with the resistance ratio 86.1. In order to determine the mechanism of this resistance, the chloroplastic ACCase cDNA from RR and SS were cloned. After compared the deduced amino acid sequences, a single isoleucine-leucine substitution at position 1781 (position number standardized to Alopecurus myosuroides (black grass)) was found. This mutation was identical with the formerly reported results in R population of other weed species. In the allele-specific PCR assay, we found RR and SS were all homozygous. All the copies in RR were Leu1781 and SS were Ile1781.?????
	TO cite this article:HUAN Zhibo,WANG Jinxin. Molecular analysis of quizalofop-resistant Echinochloa crusgalli reveals a Leu-1781-Ile mutation[OL].[ 6 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4414727


	7. Dissipation of carbendazim and chloramphenicol alone and in combination, and their effects on soil fungi:bacteria and soil enzyme activities
	Yan Hu,Dong Bin,Yu Yunlong
	Agronomy 17 December 2010
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	Abstract:The dissipation of carbendazim and chloramphenicol alone and in combination, and their effects on soil fungi:bacteria and soil enzyme activities were investigated. The results revealed that carbendazim dissipation was little affected by chloramphenicol, whereas chloramphenicol dissipation was found to be retarded significantly by the presence of carbendazim. The inhibitory effect of carbendazim on fungal:bacterial ratio and chloramphenicol on neutral phosphatase was enlarged by the presence of chloramphenicol and carbendazim, respectively. The increase in soil catalase and urease activities caused by carbendazim was partially eliminated by the presence of chloramphenicol. Little interaction between carbendazim and chloramphenicol was observed for their influence on soil invertase. The results obtained in this study suggested that combination of fungicide and antibiotic may alter their individual behavior in soil, and their effects on soil enzymes.
	TO cite this article:Yan Hu,Dong Bin,Yu Yunlong. Dissipation of carbendazim and chloramphenicol alone and in combination, and their effects on soil fungi:bacteria and soil enzyme activities[OL].[17 December 2010] http://en.paper.edu.cn/en_releasepaper/content/4398636


	8. The effects of luteolin on phenoloxidase and the growth of Spodotera exigua (Hübner) larvae (Lepidoptera: Noctuidae)
	Xue Chaobin,Luo Wanchun
	Agronomy 14 December 2010
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	Abstract:The effects of luteolin on phenoloxidase (PO) from Spodotera exigua larvae were investigated with the use of a microtitration assay in the present paper. The results showed that luteolin could inhibit the activity of PO and concentration of this inhibitor that led to a 50% (IC50) activity reduction was estimated to be 0.47 mmol/L. The inhibitory kinetics was analyzed by Lineweaver-Burk plots and the compound was found to be a reversible competitive inhibitor with the Ki of 13.11 mmol/L. The compound was obviously toxic against the larvae (≤3rd instar) of Spodoptera exigua. The highest inhibitory percentage in the older larvae (≥3rd instar) was greater than 60% with luteolin treatment. The pupation rate and percentage of eclosion was also evidently reduced with luteolin treatment by the ingestion method in this investigation.
	TO cite this article:Xue Chaobin,Luo Wanchun. The effects of luteolin on phenoloxidase and the growth of Spodotera exigua (Hübner) larvae (Lepidoptera: Noctuidae)[OL].[14 December 2010] http://en.paper.edu.cn/en_releasepaper/content/4397554

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