- Chinese︱Feedback︱Save this page
Get RSS feed
Check out RSS, or use RSS reader to subscribe this item 
Confirmation
Authentication email has already been sent, please check your email box: and activate it as soon as possible.
You can login to My Profile and manage your email alerts.
If you haven’t received the email, please:
- Login︱Register
|
|
|
 |
|
||
| There are 60 papers published in subject: since this site started. | |||
| Results per page: | 60 Total, 6 Pages | << First < Previous 3 4 5 6 |
| Select Subject |
| Select/Unselect all | For Selected Papers |
Saved Papers
Please enter a name for this paper to be shown in your personalized Saved Papers list
|
 |
| 1. Differential expression patterns of Toll-like receptor 4 at the maternal-fetal interface of pregnant Yorkshire and Meishan pigs | |||
| Liu Huazhen,Zhang Gaoying,Peng Kemei,Liu Yinxue,Yu Mei | |||
Animal Husbandry, Veterinary Medicine 31 December 2011
|
|||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:Toll-like receptor 4 (TLR4) has been suggested to play a very important role in modulation of immunological tolerance in the human female reproductive tract. To better understanding of functions of TLR4 in establishment and maintenance of pregnancy in pigs, the study was performed to compare the expression patterns of TLR4 at maternal-fetal interface of pregnant Yorkshire and Meishan pigs. The uteri from Yorkshire and Meishan gilts on days 26 and 50 of gestation were obtained respectively. Immunohistochemical analysis indicated that TLR4 was detected in uterine epithelium and trophoblast of these two types of pigs on days 26 and 50 of gestation. In Yorkshire gilts, the expression of TLR4 was higher in uterine epithelium than that in trophoblast on day 26 of gestation, while it was lower in uterine epithelium than that in trophoblast on day 50 of gestation. In Meishan pigs, the expression of TLR4 in trophoblast was higher than that in uterine epithelium on either day 26 or day 50 of gestation and TLR4 was found to be mainly present in the cells located at the bottom of trophoblast on day 50 of gestation. Compared with Meishan pigs, TLR4 was abundant in uterine epithelium of Yorkshire pigs on days 26 and 50 of gestation, yet it was decreased in trophoblast of Yorkshire pigs on day 50 of gestation. The results indicated that TLR4 at the maternal-fetal interface is expressed in differential patterns between Yorkshire and Meishan pigs and provided novel information about the functions of TLR4 in pig pregnancy. | |||
| TO cite this article:Liu Huazhen,Zhang Gaoying,Peng Kemei, et al. Differential expression patterns of Toll-like receptor 4 at the maternal-fetal interface of pregnant Yorkshire and Meishan pigs[OL].[31 December 2011] http://en.paper.edu.cn/en_releasepaper/content/4458651 | |||
| 2. Nitrate Reduction to Inhibit Ruminal Methanogenesis and to Improve Microbial Nitrogen Synthesis | |||
| Meng Qingxiang,Ren Liping,Zhou Zhenming,Lin Miao,Shi Caixia | |||
Animal Husbandry, Veterinary Medicine 13 December 2011
|
|||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:The majority of research showed that nitrate can be administered to ruminants as a sole or major source of fermentable nitrogen. The research evidence leaves little doubt that nitrate, when included at sufficient concentrations in a diet so as to maintain optimum fermentation rate, can largely prevent enteric methane production and greately improve rumen microbial protein synthesis. Several microbial enzymes are characterised as nitrate and nitrite reductases and often are present in the anaerobic microorganisms. In practice, the limitation to nitrate use in a feed is its association with nitrite poisoning. However, nitrite accumulation appears to only occur when relatively large quantities of nitrate are suddenly introduced directly into the rumen of animals not accustomed to nitrate in their feed. Under normal situations, nitrate is not toxic to ruminant animals, but nitrite is detrimental to well being. The toxicity of nitrate is related to many factors, such as animal type, adaptation period, nitrate dosage, feed type and feed rate. Dissimilatory reduction of nitrate to nitrite and assimilatory reduction of nitrite to ammonia are pathways of nitrate metabolism in the rumen, and multiple nitrate/nitrite reductases are involved in the reduction of nitrate to nitrite then to ammonia. Nitrate addition can inhibit methane production in the rumen with the mechanisms that are inhibition of intermedial by-products during nitrate denitrification, and electron competition between nitrate and nitrite reducing bacteria and methanogenic organisms. Some factors influencing nitrate used in ruminant feeds are also discussed in detail. | |||
| TO cite this article:Meng Qingxiang,Ren Liping,Zhou Zhenming, et al. Nitrate Reduction to Inhibit Ruminal Methanogenesis and to Improve Microbial Nitrogen Synthesis[OL].[13 December 2011] http://en.paper.edu.cn/en_releasepaper/content/4455495 | |||
| 3. Paradigm of Time-sequence Development of the Intestine of Suckling Piglets with microarray | |||
| SUN Yunzi,YU Bing,ZHANG Keying,HE Jun,CHEN Xijian,CHEN Daiwen | |||
| Animal Husbandry, Veterinary Medicine 22 February 2011
|
|||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:The interaction of the genes involved in intestinal development is the molecular basis of the regulatory mechanisms of intestinal development. The objective of this study was to identify the significant pathways and key genes that regulate intestinal development in Landrace piglets, and then to elucidate their rules of operation. The differential expression of genes related to intestinal development during suckling was investigated using a porcine genome array. Time sequence profiles were then analyzed for the differentially expressed genes to obtain significant expression profiles. Subsequently, the most significant profiles were assayed using Gene Ontology categories, pathway analysis, network analysis, and analysis of gene co-expression to unveil the main biological processes, the significant pathways, and the effective genes, respectively. In addition, quantitative real-time PCR was carried out to verify the reliability of the results of the analysis of the array. The results showed that more than 8000 differential expression transcripts were identified using microarray technology. Among the 30 significant model profiles obtained, profiles 66 and 13 were the most significant. Analysis of profiles 66 and 13 indicated that they were mainly involved in immunity, metabolism, and cell division or proliferation. Among the most effective genes in these two profiles, CN161469, which is similar to methylcrotonoyl-Coenzyme A carboxylase 2 (beta), and U89949.1, which encodes a folate binding protein, had a crucial influence on the co-expression network. | |||
| TO cite this article:SUN Yunzi,YU Bing,ZHANG Keying, et al. Paradigm of Time-sequence Development of the Intestine of Suckling Piglets with microarray[OL].[22 February 2011] http://en.paper.edu.cn/en_releasepaper/content/4411626 | |||
| 4. Screening and Identification of Differentially Expressed Genes in Goose Hepatocytes Exposed to Free Fatty Acid | |||
| Pan Zhixiong,Wang Jiwen | |||
| Animal Husbandry, Veterinary Medicine 19 November 2010
|
|||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:The overaccumulation of triglycerides (TG) in hepatocytes induced hepatic steatosis, however, little is known about the mechanism of goose hepatic steatosis. The aim of this study was to define an experimental model of hepatocellular steatosis with TG overaccumulation and minimal cytotoxicity, using a mixture of various proportions of oleate and palmitate free fatty acids (FFAs) to induce fat-overloading, then using suppressive subtractive hybridization (SSH) and a quantitative PCR approach to identify genes with higher or lower expression levels after the treatment of cells with FFA mixtures as compared with untreated cells. Overall, 502 differentially expressed clones, representing 21 novel genes and 87 known genes, were detected by SSH. Based on functional clustering, up- and down-regulated genes were mostly related to carbohydrate and lipid metabolism, enzyme activity and signal transduction. The expression of 20 selected clones involved with carbohydrate and lipid metabolism pathways was further studied by quantitative PCR. The data indicated that six clones similar to the genes ChREBP, FoxO1, apoB, IHPK2, KIF1B and FSP27, which participate in de novo synthesis of fatty acid and secretion of very low density lipoproteins (VLDLs), had significantly lower expression levels in the hepatocytes of treated with FFA mixtures. Meanwhile, thirteen clones similar to the genes DGAT-1, ACSL1, DHRS7, PPARα, L-FABP, DGAT-2, PCK, ACSL3, CPT-1, A-FABP, PPARβ, MAT and ALDOB had significantly higher expression levels in the hepatocytes of treated with FFA mixtures. These results suggest that several metabolic pathways are altered in goose hepatocytes, which may be useful for further research into the molecular mechanism of goose hepatic steatosis. | |||
| TO cite this article:Pan Zhixiong,Wang Jiwen. Screening and Identification of Differentially Expressed Genes in Goose Hepatocytes Exposed to Free Fatty Acid[OL].[19 November 2010] http://en.paper.edu.cn/en_releasepaper/content/4392163 | |||
| 5. Regulation of expression and activity of selenoenzymes by different forms and concentrations of selenium in primary cultured chicken hepatocytes | |||
| Wu Xianshi,Wei Chengwu,Pan Cuiling,Duan Ying,Huang Kehe | |||
| Animal Husbandry, Veterinary Medicine 09 November 2010
|
|||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:In this study, the effects of different forms and concentrations of Se on the regulation of glutathione peroxidase (GPx) activity and mRNA levels of phospholipid hydroperoxide GPx (GPx4) and type I deiodinase (D1) in chicken hepatocytes were evaluated. Primary cultured chicken hepatocyte monolayers derived from male White Leghorn chickens (aged 30-40 days) were incubated for 24 h with 0 (control), 0.5, 1, 1.5, 2, 3, 4 or 5 μmol/l of Se from DL-selenomethionine (Se-Met), Kappa-selenocarrageenan (Se-Car) or sodium selenite (Na2SeO3). Compared with controls, significant increases of GPx activity were observed in all hepatocytes treated with Se except for the hepatocytes treated with 5 μmol/l of Na2SeO3, with maximal effects at 2 μmol/l of Se-Met or Se-Car and 1.5 μmol/l of Na2SeO3, respectively. Significant decreases of GPx4 mRNA were observed in all hepatocytes treated with Se (vs. control). D1 mRNA was significantly increased in all groups treated with Se (vs. control), with maximal effects at 1.5 μmol/l of Se-Met and 0.5 μmol/l of Se-Car or Na2SeO3, respectively. Se-Met at doses of 1.5-5 μmol/l had greater effect on D1 mRNA than Se-Car and Na2SeO3 at equivalent doses. After resulting in the maximal effect, higher Se supplementation led to a dose-dependent reduction of GPx activity and D1 mRNA in all hepatocytes treated with Se. These results suggest that in chicken hepatocytes, the regulations of GPx and D1 of by different forms and concentrations of Se vary. | |||
| TO cite this article:Wu Xianshi,Wei Chengwu,Pan Cuiling, et al. Regulation of expression and activity of selenoenzymes by different forms and concentrations of selenium in primary cultured chicken hepatocytes[J].British Journal of Nutrition，2010，104（11）：1605-1612. | |||
| 6. Characterization and SNP Identification of Goat TYRP1 Gene | |||
| Zheng Huiqin ,Lanhui Li,Xianglong Li,Rongyan Zhou,Fengling Xuan,Jingjing Zhang,Zunan Shen,Hongbo Zhao | |||
| Animal Husbandry, Veterinary Medicine 20 April 2010
|
|||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:As one member of tyrosinase-related family, tyrosinase-related protein-1(TYRP1) has been reported to play an important role in melanin biosynthetic pathway in some species, but little is known about it in goat. In this study, almost complete goat TYRP1 gene (17554bp) encompassing complete CDS was determined, the coding region was 1614bp, distributed in 7 exons (2-8), intron 5 was found to possess GC-AG as its splice sites. Dozens of SNPs as well as simple sequence repeats (SSR) motifs were detected in the goat TYRP1 gene. Popgene analysis suggested that allele A at site 1263 and haplotype AC for g.1263A>C and g.1428C>T should be in favor of eumelanin biosynthesis, while mutation C→T at site 1428 occurred more recently in evolution and might have little effect on coat color. High deficiency of heterozygotes existed in Nanjiang Yellow Goat Black strain, Nanjiang Yellow Goat Fast Grow strain and Jining Gray Goat, indicating high inbreeding coefficient. Genetic differentiation among the 9 goat populations were significantly low for both g.1263A>C and g.1428C>T. | |||
| TO cite this article:Zheng Huiqin ,Lanhui Li,Xianglong Li, et al. Characterization and SNP Identification of Goat TYRP1 Gene[OL].[20 April 2010] http://en.paper.edu.cn/en_releasepaper/content/42134 | |||
| 7. Effects of exogenous metallothionein on several endocrine hubs of dairy cattles | |||
| Wu Lizhuan,Chen Haiyan,Zhang Bin,Li Lili,Xiao Bingnan,Chen Yuguang,Xiao Dingfu,Luo Jiajie,Wu Zongming | |||
Animal Husbandry, Veterinary Medicine 26 January 2010
|
|||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:To approach the effects of exogenous metallothionein (Zn-MT) on endocrine hubs of dairy cattles on the low-grade thermal stress, an experiment was conducted with 24 lactating cows, who were randomly allocated to groups A, B, C and D, and supplemented with 0, 6.0, 12.0 and 16.0mg Zn-MT•capita-1, respectively, by intravenous injection. The results showed that: The corrected milk yields in groups B, C and D were higher than those in groups A (P<0.05or P<0.01), the fat percentages of milk was improved significantly (P<0.05). The secretory capacity of INS in control group was lower than in experimental groups, especially in group B in 30d（P<0.01）; the secretory capacity of IGF-Ⅰand T3 in groups B, C and D were higher than those in groups A (P<0.05or P<0.01), and in 45d, the same capacity in group C was the highest among all these numbers. The FSH in groups B and C constantly maintained low level, but the range of variation of FSH in group A was 4 times as same as in group C; The secretory capacity of Prog and E2 in experimental groups were higher than those of group A in anaphase (30~45d)(P<0.05); and the PRL in groups B, C and D were higher significantly than those in groups A (P<0.01), and the effect was faint in anaphase (P>0.05). Exogenous Zn-MT had the best effects on the thermoresistance of diary cattle 12.0mg•capita-1 by intravenous injection every 30~45 days. On one hand, the speed of catabolism and quantity of heat production in bodies were reduced by Zn-MT through adjusting secretory volume of endocrine hormone which is related to energy metabolism, on the other hand, to approach the healthful effects of thermoresistance, the gonadal hormones were controlled amplitude of variation, and on the same time, the secretory volume of PRL was improved by Zn-MT, which resulted in physiological status which was varied on balance for diary cattle. | |||
| TO cite this article:Wu Lizhuan,Chen Haiyan,Zhang Bin, et al. Effects of exogenous metallothionein on several endocrine hubs of dairy cattles[OL].[26 January 2010] http://en.paper.edu.cn/en_releasepaper/content/39410 | |||
| 8. Numerical simulation of inspiratory airway in nasal cavity of Bactrian camels (Camelus bactrianus) | |||
| Wang Hongju,Bai Zhongtian ,Wang Jianlin ,Gao Chengjuan | |||
| Animal Husbandry, Veterinary Medicine 27 September 2008
|
|||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:To investigate the flow patterns in nasal cavity of Bactrian camel (Camelus bactrianus), we construct an anatomically accurate finite element model of its left nasal cavity from CT scans of a healthy adult nose. By numerically solving the steady-state Navier-Strokes and continuity equations, we study the flow distribution and the complete velocity field for inspiration throughout the nasal cavity under physiological flow rates of breathing. During inspiration, the highest velocity occurred along the nasal floor. | |||
| TO cite this article:Wang Hongju,Bai Zhongtian ,Wang Jianlin , et al. Numerical simulation of inspiratory airway in nasal cavity of Bactrian camels (Camelus bactrianus)[OL].[27 September 2008] http://en.paper.edu.cn/en_releasepaper/content/24420 | |||
| 9. The Application and Assessment of Ketamine–Xylidinothiazoline Combinations for Anaesthesia in Rhesus Monkey | |||
| xiao jianhua,Wang Hongbin | |||
| Animal Husbandry, Veterinary Medicine 07 January 2008
|
|||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:To access the different effects of between ketamine and ketamine-Xylidinothiazoline in anesthesia of rhesus monkeys. 20 Rhesus Macaque were anesthesia with tow kinds methods in this experiment. The anesthesia effects were evaluated during anesthesia. At the same time the cardiovascular parameters, and respiration parameters were monitored with Datex circulation, respiration monitor and multi-functional physiologic graphed SJ-42 continuously and dynamically. The results showed that the effects of anesthesia was good, it reach the surgical anesthetic depth. The relation between analgesia, sedation and muscle relaxation were balanced. The induction time were 5.0±1.6min and the anesthesia maintained 55.3±6.6min in all. Furthermore the compound anesthetic has little effects on the physiology function of Rhesus Macaque and heart rate, blood pressure, blood oxygen saturation, body temperature, respiratory rate maintain in normal physiological range during anesthesia. It was evident that the compound anesthetic can meet the clinical needs. | |||
| TO cite this article:xiao jianhua,Wang Hongbin . The Application and Assessment of Ketamine–Xylidinothiazoline Combinations for Anaesthesia in Rhesus Monkey[OL].[ 7 January 2008] http://en.paper.edu.cn/en_releasepaper/content/17840 | |||
| 10. Effects of Crude Enzyme Preparation Supplemented | |||
| Xiaojie Ai,Zhengkang Han | |||
| Animal Husbandry, Veterinary Medicine 09 December 2005 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:Twenty four 7-day-old crossbred goslings (Chuan white×Tai lake) were divided randomly into control and treatment groups. Enzyme preparation was added in the diet of treatment group at dosage of 1 g/kg. The blood samples were collected from 60 day-old goslings via the wing vein. During the fifty-three days experiment, body weights were adopted measured each week, related hormone contents and biochemical parameters in plasma were determined. The results were as following:(1) Compared with control, body weight of treatment group were significantly higher (P<0.05) since 3-week-old goslings. The weight gains were significant higher (P<0.05) in 4~7 week-old goslings too, feed intake were increased and the “feed: body weight gain” was decreased; (2) Compared with control, growth hormone (GH) and insulin-like growth factor-I (IGF-I) levels were dramatically enhanced by 36.62 % (P<0.01) and 66.55 % (P<0.01) respectively, while the level of β-endorphin (β-END) decreased by 51.66 % (P<0.01); (3) Thyroid-stimulating hormone (TSH) and 3,3\ | |||
| TO cite this article:Xiaojie Ai,Zhengkang Han. Effects of Crude Enzyme Preparation Supplemented [J].Journal of Shanghai Jiaotong University(Science) ，2005年，E-10卷，sup1期，115 ~ 119 | |||
| Select/Unselect all | For Selected Papers |
Saved Papers
Please enter a name for this paper to be shown in your personalized Saved Papers list
|
|
 |
|
||||||||||||||
| Results per page: | 60 Total, 6 Pages | << First < Previous 3 4 5 6 |
About Sciencepaper Online |
Privacy Policy |
Terms & Conditions |
Contact Us
Technology Partner - CERNET