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1. Molecular cloning, characterization and inhibitory effect on B-cell survival of BAFF-R, a novel receptor of TNFR family in horse (Equus caballus) | |||
WU Haitao,KE Zhen,ZHANG Jianrong,XU Xinzhou,LIU Meng,ZHANG Shuangquan | |||
Animal Husbandry, Veterinary Medicine 07 February 2012 | |||
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Abstract:B-cell survival depends on signals induced by B-cell activating factor (BAFF) binding to its receptor (BAFF-R). In mice, mutations in BAFF or BAFF-R cause B-cell lymphopenia and antibody deficiency. However, the information about horse is poorly understood. Here, We report the identification and analysis of a novel horse TNF receptor related to B-cell suivival, BAFF-R. The horse BAFF-R gene spans 1444 bp, including three exons and located on chromosome 28. The horse BAFF-R protein is composed of 183 aa, with the calculated molecular weight 19 kDa and isoelectric point 6.12. As a type Ⅲ transmembrane protein, its extracellular domain has one cysteine-rich domain (CRD) containing four cysteine residues, and cytoplasmic domain exists the TRAF3 binding domain. The genetic relationship between horse and dog is the closest by the phylogenetic analysis of BAFF-R amino acids. Moreover, recombinant BAFF-R-Fc protein not only binds to B-cell, but also inhibits B-cell survival induced by BAFF in vitro. These studies provide the basis for investigation on its roles in regulating B lymphocyte survival and related immune diseases in horse. | |||
TO cite this article:WU Haitao,KE Zhen,ZHANG Jianrong, et al. Molecular cloning, characterization and inhibitory effect on B-cell survival of BAFF-R, a novel receptor of TNFR family in horse (Equus caballus)[OL].[ 7 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4465365 |
2. Regulation of expression and activity of selenoenzymes by different forms and concentrations of selenium in primary cultured chicken hepatocytes | |||
Wu Xianshi,Wei Chengwu,Pan Cuiling,Duan Ying,Huang Kehe | |||
Animal Husbandry, Veterinary Medicine 09 November 2010 | |||
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Abstract:In this study, the effects of different forms and concentrations of Se on the regulation of glutathione peroxidase (GPx) activity and mRNA levels of phospholipid hydroperoxide GPx (GPx4) and type I deiodinase (D1) in chicken hepatocytes were evaluated. Primary cultured chicken hepatocyte monolayers derived from male White Leghorn chickens (aged 30-40 days) were incubated for 24 h with 0 (control), 0.5, 1, 1.5, 2, 3, 4 or 5 μmol/l of Se from DL-selenomethionine (Se-Met), Kappa-selenocarrageenan (Se-Car) or sodium selenite (Na2SeO3). Compared with controls, significant increases of GPx activity were observed in all hepatocytes treated with Se except for the hepatocytes treated with 5 μmol/l of Na2SeO3, with maximal effects at 2 μmol/l of Se-Met or Se-Car and 1.5 μmol/l of Na2SeO3, respectively. Significant decreases of GPx4 mRNA were observed in all hepatocytes treated with Se (vs. control). D1 mRNA was significantly increased in all groups treated with Se (vs. control), with maximal effects at 1.5 μmol/l of Se-Met and 0.5 μmol/l of Se-Car or Na2SeO3, respectively. Se-Met at doses of 1.5-5 μmol/l had greater effect on D1 mRNA than Se-Car and Na2SeO3 at equivalent doses. After resulting in the maximal effect, higher Se supplementation led to a dose-dependent reduction of GPx activity and D1 mRNA in all hepatocytes treated with Se. These results suggest that in chicken hepatocytes, the regulations of GPx and D1 of by different forms and concentrations of Se vary. | |||
TO cite this article:Wu Xianshi,Wei Chengwu,Pan Cuiling, et al. Regulation of expression and activity of selenoenzymes by different forms and concentrations of selenium in primary cultured chicken hepatocytes[J].British Journal of Nutrition,2010,104(11):1605-1612. |
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