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1. Markers in diagnosis of lung cancer: Several common detection methods | |||
LIN Liquan,ZHANG Xi,ZOU Yingchang,LU Yanli,WANG Ping,CHEN Xing | |||
Basic Medicine 09 September 2015 | |||
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Abstract:There's an old saying in China that people turn pale at the mention of a tiger. Today, people will turn pale at the mention of lung cancer because of its high morbidity and high mortality. Fortunately, studies have found lots of markers that can be applied in observational and analytic epidemiology, randomized clinical trials, screening, diagnosis and prognosis of lung cancer. Those markers are including proteins in serum, volatile organic compounds (VOCs) in exhaled breath, exhaled breath condensates (EBCs), gene expression, microRNAs, and medical imaging indices. At the same time, various detection techniques were used to measure the markers. With the effort of researchers, a better detection of lung cancer will be realized by combining different markers in different stages. Developing detection techniques will bring novel machines that are more rapid, more accurate, more robust and more comfort for the diagnosis of lung cancer patient. | |||
TO cite this article:LIN Liquan,ZHANG Xi,ZOU Yingchang, et al. Markers in diagnosis of lung cancer: Several common detection methods[OL].[ 9 September 2015] http://en.paper.edu.cn/en_releasepaper/content/4654285 |
2. Neural ensemble sparse coding during working memory task in rat prefrontal cortex | |||
Xu Yunhua,Bai Wenwen,Tian Xin | |||
Basic Medicine 27 December 2010 | |||
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Abstract:The neural ensemble aims to provide a mechanistic explanation of how groups of neurons acting together during a cognitive processing. It is significant to expressing neural ensemble at a higher precision when neural population activity data are recoded from experiment. In this article, we propose the use of sparse coding as a new methodology to address this issue by recording the activities of neurons in the rat prefrontal cortex during the working memory task in Y-maze. The time information of neural activity is summarized into bin counts using 200 milliseconds windows, and a sparse code for the bin-count matrix is found by means of a linear generative model. The meaningful components are extracted to reconstruct the input by an inverse of the sparsifying transform. None of the feature components are ignored or missed, as the number of the source components is greater than that of neurons. The reconstructed sparse neuronal activities are compared with rate coding. The recording twenty cases dealt show that, using sparse coding, it is possible to identify spatiotemporal patterns of neural activity in the form of reconstructed signal. The results indicate that the spatiotemporal location of neural ensemble could be more precisely detected, using sparse coding. | |||
TO cite this article:Xu Yunhua,Bai Wenwen,Tian Xin. Neural ensemble sparse coding during working memory task in rat prefrontal cortex[OL].[27 December 2010] http://en.paper.edu.cn/en_releasepaper/content/4401529 |
3. A PCR-based Technology for Quickly Screening of gDNA Library | |||
Zhao Yongxiang | |||
Basic Medicine 03 July 2006 | |||
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Abstract:Objective: To explore the feasibility of quickly screening of gDNA library with PCR technique. Methods: We adopted porcine α-1,3GT cDNA fragment as the probe, used the primers synthesized by the specific sequence on cDNA to carry out α-1,3GT gene screening of porcine gDNA library by combining PCR and in situ plaque hybridization, and then performed enzymic digestion, southern blot, sequencing and fluorescence in situ hybridization for location. Results: After having finished one-time hybridization and one-time PCR, we obtained 7 positive monoclones with very strong signals, and each insert length of them is over 8kb, including the third intron. Moreover, 3 tested clones among them contain the third and fourth exons according to the sequencing results, and FISH mapped the inserts of the 3 clones to pig chromosome 1q2.10-q2.11. Conclusion: PCR could be applicable to the quick screening of DNA library and much simpler than the conventional in situ plaque hybridization only used. | |||
TO cite this article:Zhao Yongxiang. A PCR-based Technology for Quickly Screening of gDNA Library[OL].[ 3 July 2006] http://en.paper.edu.cn/en_releasepaper/content/7438 |
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