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| There are 11 papers published in subject: > since this site started. | |||
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| 1. Asparagine linked glycosylation 8 alpha 13 glucosyltransferase homolog (S. cerevisiae) (ALG8) inhibited TGF beta receptor cognition mechanism | |||
| HONG Tao,WANG Lin | |||
Basic Medicine 12 June 2022
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| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:Asparagine linked glycosylation 8 alpha 13 glucosyltransferase homolog (S. cerevisiae) (ALG8) feedback-inhibited beta-transducin repeat containing E3 ubiquitin protein ligase (BTRC) and ALG8 downstream-inhibited karyopherin subunit beta 1 (KPNB1) of TGF beta receptor knowledge and molecular subnetworks were constructed from ALG8 feedback-inhibited and downstream-inhibited networks based on integrating microarray significance analysis (SAM), SPSS correlation coefficient Pearson, gene (protein) reconstruction network (GRNInfer) and the Database for Annotation, Visualization and Integrated Discovery (DAVID). ALG8 feedback-inhibited BTRC of TGF beta receptor mechanism was identified upstream inositol 1 4 5-trisphosphate receptor type 1(ITPR1) of BM CD105+Endothelial_3rd, endoplasmic reticulum membrane, fetalbrain_3rd; microtubule associated protein 1B(MAP1B_2) of structural molecule activity; feedback calmodulin binding transcription activator 1(CAMTA1) of ovarian tumor_disease_3rd, ovary_normal_3rd; F-box and leucine-rich repeat protein 5(FBXL5) of protein ubiquitination, ubiquitin protein transferase activity; KIF3A of ATP binding; downstream C1D nuclear receptor corepressor(C1D) of ovarian tumor_disease_3rd, RNA binding; microtubule associated protein 1B(MAP1B_1) of structural molecule activity. ALG8 downstream-inhibited TGF beta receptor mechanism was identified upstream cyclin-dependent kinase 17(PCTK2) of ATP binding; feedback no result; downstream DAZ interacting zinc finger protein 3(DZIP3) of BM CD105+Endothelial_3rd, fetalbrain_3rd, ovarian tumor_disease_3rd, ovary_normal_3rd, ubiquitin protein transferase activity, RNA binding; iron-sulfur cluster assembly 1(ISCA1) of structural molecule activity; SEL1L ERAD E3 ligase adaptor subunit (SEL1L) of endoplasmic reticulum membrane; DDB1 and CUL4 associated factor 7(WDR68) of protein ubiquitination. We put forward ALG8 inhibited TGF beta receptor and cognition mechanism through activation of ATP binding or RE structural molecule or RNA binding or protein ubiquitination effect on fetalbrain, ovarian tumor_disease and normal, BM CD105+Endothelial. | |||
| TO cite this article:HONG Tao,WANG Lin. Asparagine linked glycosylation 8 alpha 13 glucosyltransferase homolog (S. cerevisiae) (ALG8) inhibited TGF beta receptor cognition mechanism[OL].[12 June 2022] http://en.paper.edu.cn/en_releasepaper/content/4757972 | |||
| 2. Preparation of keratin/chitosan sponge and its application performance evaluation | |||
| YAN Rongrong,SHI Jinsong | |||
| Basic Medicine 13 May 2022
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| Abstract:Uncontrolled bleeding leads to a higher fatality rate in the situation of surgery, traffic accidents and warfare. Traditional hemostatic materials such as bandages are not ideal for uncontrolled or incompressible bleeding. Therefore, it is of great significance to develop a new medical biomaterial with excellent rapid hemostatic effect. As a potential biomedical material, keratin has been developed and paid attention in tissue engineering fields such as promoting wound healing and nerve repair. Herein, a novel keratin/chitosan (K/C) sponge was prepared to achieve rapid hemostasis. The characterizations of K/C sponge were investigated, including SEM, TGA, liquid absorption and porosity, showing that the high porosity up to 90.12 ± 2.17% resulted in an excellent blood absorption. The cytotoxicity test and implantation experiment proved that the K/C sponge was biocompatible and biodegradable. Moreover, the prepared K/C sponge showed better hemostatic performance than chitosan sponge (CS) and the commercially available gelatin sponge in both rat tail amputation and liver trauma bleeding models. Further experiments showed that K/C sponge plays a hemostatic role through the endogenous coagulation pathway, thus shortening the activated partial thromboplastin time (APTT) effectively. Therefore, this study provided a novel K/C sponge which can be served as a promising biomedical hemostatic material. | |||
| TO cite this article:YAN Rongrong,SHI Jinsong. Preparation of keratin/chitosan sponge and its application performance evaluation[OL].[13 May 2022] http://en.paper.edu.cn/en_releasepaper/content/4757746 | |||
| 3. Simultaneous assay of platelet adhesion at multiple shear rates within a single microfluidic channel | |||
| Zeng Lisha,Yang Jun | |||
| Basic Medicine 01 April 2016
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| Abstract:Cardiovascular diseases are currently the major causes of mortality in the world, especially in developed nations. As a predominant one, thrombosis is the platelet aggregation induced by a high shear rate. Platelet aggregation assay can clarify the occurrence mechanism of thrombosis, as well as used as an important tool in the clinical diagnosis, personalized treatment, and screening of anticoagulants. Thus, relevant studies attracted considerable attention. As an important step in platelet aggregation, platelet adhesion and its detection are also attracted intensive concern. Thus, some analytical methods have been developed for platelet adhesion assay, and the impact of shear rate is one of the focuses. Compared with other devices, biosensors can give a more accurate result within a shorter time. Furthermore, some biosensors can achieve real-time analysis. However, only one or several shear rates can be tested at the same time, which may decrease the analytical efficiency. Meanwhile, in most cases, only the average platelet-adhesion effect within a reactor is detection, and the impact of the distribution of shear rates is improperly neglected. In this study, a microfluidic device with a single channel is designed and fabricated for platelet adhesion assay. When the platelet-rich plasma flows through the collagen-modified sensing surface of the channel bottom, the interaction between platelets and collagen molecules on the entire surface can be simultaneously monitored by using a surface plasmon resonance imaging (SPRi) system. A gradient of the shear rate (0-546) could be formed within the channel by choosing a suitable depth-to-width ratio (1:5), so platelet adhesion at multiple shear rates could be monitored simultaneously. This method enables the measurement of the adhesion process of unlabeled platelets on the entire sensing surface, in vitro, at multiple shear rates. Such a system can obtain more accurate platelet adhesion result at a given shear rate than traditional methods. Furthermore, in an individual operation, platelet adhesion can be repeatedly tested at multiple points with an equal shear rate, so a much higher analytical efficiency can also be achieved. | |||
| TO cite this article:Zeng Lisha,Yang Jun. Simultaneous assay of platelet adhesion at multiple shear rates within a single microfluidic channel[OL].[ 1 April 2016] http://en.paper.edu.cn/en_releasepaper/content/4682815 | |||
| 4. Low SNR signal automatic quantification in magnetic resonance spectroscopy data | |||
| LI Yao | |||
| Basic Medicine 28 January 2015
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| Abstract:The quantification of magnetic resonance spectroscopy (MRS) signals remains challenging due to the low signal-to-noise ratio (SNR) of data. Time-domain quantification methods highly require user interactions, which reduce the reproducibility of the data quantification results. The goal of our work is to design a systematic methodology for automated quantification of MRS signals with low SNRs. We used Hankel singular value decomposition (HSVD) algorithm in our signal estimation step, along with extraction and reduction filter (ER-filter) as a frequency selective technique in the preprocessing step in order to avoid the interferences from nuisance peaks. In the automatic model order selection problem of HSVD, we implemented three strategies based on reconstruction residue measurement or information theoretic criteria. The performances were evaluated in terms of detection rate and relative root mean squared error (RRMSE). The simulations were run on both synthesized and semi-synthesized data. We tested the strategies in two cases, i.e., without and with an interfering signal nearby the signal of interest. It is shown that the minimum description length with condition (MDLcon) based methodology we proposed performs the best and can obtain reliable estimation performance (RRMSE<40%) when signal SNR is larger than -18dB, with a detection rate above 72.44%. The performance is consistent when the interfering signal is 0.08ppm separated. Overall, the MDLcon based automated MRS signal quantification methodology provides an effective way for low SNR MRS signal estimation and detection. Our work may shed light on automatic MRS signal quantification in clinical applications when the corresponding metabolite concentration is low. | |||
| TO cite this article:LI Yao. Low SNR signal automatic quantification in magnetic resonance spectroscopy data[OL].[28 January 2015] http://en.paper.edu.cn/en_releasepaper/content/4631195 | |||
| 5. Granger Causality relationships between SNMF components of LFPs during SD rats working memory task | |||
| Liu Xu,Bai Wenwen,Liu Tiaotiao,Yi Hu,Tian Xin | |||
| Basic Medicine 09 May 2013
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| Abstract:Recent studies have applied Granger causality to multivariate population recordings such as local field potentials (LFPs) or electroencephalography (EEG), which were usually observed signals. In this study, we used Granger causality to analyze relationships between source components of LFPs recorded from microelectrode arrays targeting the rats' prefrontal cortex when rats were at rest (rest group) and during Y-maze working memory task (working memory group). LFPs signals are assumed to be linear mixtures of source components. Furthermore, evidence indicates sparse coding of neural systems. Therefore, we used sparse non-negative matrix factorization (SNMF) to separate blind source components for LFPs. Then we used Granger causality to determine the strength and direction of relationships between source components.Our results showed that causal connectivity indices 'causal density' of the new networks consisting of source components strengthened than LFPs networks. By studying the new networks of rest group and working memory group, we found that the causal connectivity of working memory group was more enhanced than rest group.This paper presents a thought of applying Granger causality to source components, which may be especially useful to reveal essential causal interactions of neural networks. | |||
| TO cite this article:Liu Xu,Bai Wenwen,Liu Tiaotiao, et al. Granger Causality relationships between SNMF components of LFPs during SD rats working memory task[OL].[ 9 May 2013] http://en.paper.edu.cn/en_releasepaper/content/4539143 | |||
| 6. Tumor targeting polymeric micelles loaded with ultrasmall superparamagnetic iron oxide | |||
| Xu Qilan,Ruan renxu,Hong Guobin | |||
| Basic Medicine 03 September 2012
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| Abstract:Targeted delivery of contrast agents is a highly desirable strategy for enhancing diagnostic efficiency and reducing side effects and toxicity. Water-soluble and tumor-targeting superparamagnetic iron oxide nanoparticles (SPIONs) were synthesized by loading hydrophobic SPIONs into micelles assembled from an amphiphilic block copolymer poly(ethylene glycol)-poly(ε-caprolactone) (PEG-PCL) bearing folate in the distal ends of PEG chains. Compared to the water-soluble SPIONs obtained by small molecular surfactant coating, ultrasmall SPIONs encapsulation with PEG-PCL micelles (PEG-PCL-SPIONs) simultaneously increases transversal (r2) and decreases longitudinal (r1) magnetic resonance (MR) relaxivities of water proton in micelle solution, leading to a notably high r2/r1 ratio up to 78, which makes the PEG-PCL-SPIONs a highly sensitive MRI T2 contrast agent. The mean size of folate attached SPION micelles (Fa-PEG-PCL-SPIONs) is 44 ? 3nm on average, ideal for in vivo MRI applications in which long circulation is greatly determined by small particle size and is highly desirable. Prussian blue staining of Bel 7402 cells over-expressing folate receptors, after incubation with micelle-containing medium, demonstrated that folate functionalization of the magnetic particles significantly enhanced their cell uptake. The potential of Fa-PEG-PCL-SPIONs as a potent MRI probe for in vivo tumor detection was assessed. At 3h after intravenous injection of the Fa-PEG-PCL-SPIONs solution into mice bearing subcutaneous xenografts of human Bel 7402 hepatoma , a 41.2% signal intensity decrease was detected in the T2-weighted MR images of the tumor, indicating the efficient accumulation of Fa-PEG-PCL-SPIONs in the tumor tissue. | |||
| TO cite this article:Xu Qilan,Ruan renxu,Hong Guobin. Tumor targeting polymeric micelles loaded with ultrasmall superparamagnetic iron oxide[OL].[ 3 September 2012] http://en.paper.edu.cn/en_releasepaper/content/4488376 | |||
| 7. Periodontal healing by periodontal ligament cell sheets in a teeth replantation model | |||
| Zhou Yefang | |||
| Basic Medicine 15 February 2012
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| Abstract:Objective: Successful transplantation of avulsed teeth is to restore the attachment and regenerate the periodontal support. Different strategies have been applied in treatment from modification of teeth storage, antibiotic usage to peridontium tissue replacement. We developed a novel periodontal ligament cell-sheet delivery system to apply on delayed replanted teeth in promoting periodontal healing in a canine model. Design: Autologous periodontal ligament (PDL) fibroblasts were isolated from extracted premolars of beagle dog. The cell-sheets were fabricated using normal culture dish after stimulation of extracellular matrix formation. Teeth were surgically extracted and attached soft tissues were removed. After root canal treatment, the root of teeth were wrapped by the PDL cell-sheets and replanted back to prior socket accordingly while teeth without cell sheets as a control. Eight weeks after surgery, the animals were sacrificed and decalcified specimens were prepared. Regeneration of periodontal tissue was evaluated through histology assay. Results: Multi-layered PDL cell-sheet could be attached on tooth root and most cells on sheet-tooth constructs were viable before replantation. Minimum clinical signs of inflammation were observed in experiment. PDL cell-sheets group show significant higher occurrence of favorable healing (88.4%) than control group with low healing (5.3%). Periodontal ligament and cememtum tissue regeneration was observed in the experimental group, and the regenerated tissues showed high collagen type III, type I and fibronectin expression. Conclusion: The periodontal ligament cell-sheets fabricated through normal cell culture dish has a potential for regeneration of periodontal ligament and may become a novel therapy for avulsed teeth replantation. | |||
| TO cite this article:Zhou Yefang. Periodontal healing by periodontal ligament cell sheets in a teeth replantation model[OL].[15 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4466549 | |||
| 8. Graphics processing unit cluster accelerated Monte Carlo simulation of photon transport in multi-layered tissues | |||
| Jiang Chao ,He Heng ,Li Pengcheng ,Luo Qingming | |||
| Basic Medicine 21 February 2011
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| Abstract:In this paper, we present a GPU cluster based Monte Carlo simulation of photon transport in multi-layered tissues. The cluster is composed of multiple computing nodes in a local area network (LAN) and each node is a personal computer equipped with one or several GPU for parallel computing. In this study, the MPI (Message Passing Interface), the OpenMP (Open Multi-Processing) and the CUDA (Compute Unified Device Architecture) technologies are employed to develop the program. It is demonstrated that this designing runs roughly N-1 times faster than that using single GPU when the GPUs within the cluster are of the same type, where N is the total number of the GPUs within the cluster. | |||
| TO cite this article:Jiang Chao ,He Heng ,Li Pengcheng , et al. Graphics processing unit cluster accelerated Monte Carlo simulation of photon transport in multi-layered tissues[OL].[21 February 2011] http://en.paper.edu.cn/en_releasepaper/content/4410954 | |||
| 9. The Hierarchical Structure Depended Osteoinduction/Osteogenesis of The Hydroxyapatite Ceramics | |||
| ZHI Wei,HUANG Peng,PENG Qian,ZHANG Cong,LUO Huitao,ZHANG Jingwei,HUANG Jing,WANG Hao,YU Haiyang,QU Shuxin,ZHU Zhuoli,DUAN Ke,WENG Jie,WANG Jianxin,XIA Tian,WU Dongbo | |||
| Basic Medicine 14 January 2011
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| Abstract:In this paper, the previously developed porous bioceramics scaffolds by accumulating hydroxyapatite (HA) spherulites which has a hierarchical structure with large dimension of 2.5~3cm in length and 1.5cm in diameter were implanted into dorsal muscle and abdominal cavity of dogs, which were deliberately created spaces within the body used as "autologous bioreactor" to grow engineered bone grafts based on the natural physiological environment. After 6 months, the dogs were sacrificed and histological analysis of explants was performed on thin paraffin sections. Lively osteogenesis was observed in pores between spherulites and in the ceramic cylinder foam, even in the micropores of HA spherulites. Bone formation by means of creeping substitution was evidently observed, in which the woven bone matrix was surrounded by osteoblasts and subsequently matured into mineralized bone while the HA bioceramics were resorbed by osteoclasts on the resorption line. Bone marrow recanalization inside the explants was obviously found. It is confirmed that an engineered bone graft similar to natural bone can been reconstructed through the novel porous bioceramics scaffold by accumulating HA spherulites in which the osteogenesis occurred in autologous bioreactors. Furthermore, the results also suggest the hierarchical structure depended osteoinduction/osteogenesis of the accumulating scaffolds. | |||
| TO cite this article:ZHI Wei,HUANG Peng,PENG Qian, et al. The Hierarchical Structure Depended Osteoinduction/Osteogenesis of The Hydroxyapatite Ceramics[OL].[14 January 2011] http://en.paper.edu.cn/en_releasepaper/content/4406625 | |||
| 10. Achieving the seeding of osteoblasts with a uniform distribution throughout the porous scaffold by accumulating hydroxyapatite spheres | |||
| GUO Laiyang,ZHI Wei,ZHU Zhuoli,LUO Huitao,PENG Qian,DUAN Ke,WENG Jie,YU Haiyang,ZHANG Cong | |||
| Basic Medicine 12 January 2011
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| Abstract:Hydroxyapatite(HA) spheres with different diameter ranges were prepared and accumulated in a HA porous tube with dimension of 1.5 cm in length and 1.2 cm in diameter as a porous scaffold for bone tissue engineering. The porosity, macro and micro porous structures of the scaffold were characterized. Three cell seeding methods were designed to distribute osteoblasts(OBs) from Sprague-Dawley (SD) rat into HA porous scaffolds: (A) direct load, (B) admixture load, and (C) transferring load in vitro. In this study, we compared the attachment, proliferation, differentiation and homogeneity of osteoblasts seeded in the porous scaffold accumulated with HA spheres in a three-dimensional (3D) culture by the designed three cell seeding methods. The OBs/Scaffold constructs were cultured for 7 days, and then the adhesion and homogeneity of distribution, proliferation, and differentiation of osteoblasts into the osteoblastic phenotypes were determined using scanning electronic microscopy (SEM), Alamar Blue assay, and alkaline phosphatase (ALP) activity. No visible negtive effects on cell morphology, proliferation or differentiation of the porous scaffolds were observed. Among the three seeding approaches, the operation of transferring achieves the best uniform distribution and higher cell seeding efficiency of osteoblasts seeded in HA porous scaffolds. After a 7-day culture of OBs/Scaffolds, there were no considerable differences of cells proliferation or differentiation seeded by the three different methods designed. | |||
| TO cite this article:GUO Laiyang,ZHI Wei,ZHU Zhuoli, et al. Achieving the seeding of osteoblasts with a uniform distribution throughout the porous scaffold by accumulating hydroxyapatite spheres[OL].[12 January 2011] http://en.paper.edu.cn/en_releasepaper/content/4406211 | |||
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