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	1. Measurement and correlation analysis of upper airway and mandible, hyoid in patients with bilateral anterior disc displacement
	Hou Yijun,Pan Xiaojing
	Clinical Medicine 23 March 2022
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	Abstract:Objective: To evaluate the presence of upper airway stenosis in patients with bilateral anterior disc displacement, and to further demonstrate the relationship between temporomandibular joint, airway, and hyoid bone. Methods: 64 patients with bilateral anterior disc displacement shown by CBCT in the joint specialty of Stomatological Hospital of Lanzhou University from June 2020 to August 2021 were selected as the patient group, 65 patients with normal joints diagnosed by arthrologists were selected as the control group. The included patients were 18-44 years old, with normal BMI index and skeletal class I. The number of patients with high angle and low angle in the experimental group was the same as that in the control group. First, Dolphin11.8 was used to measure joint space, airway volume, and cross-sectional area. It also reconstructed lateral cranial radiographs were used to measure the parameters of the mandibular and hyoid bone. last, we analyzed the correlation. Results: There was no difference in segmentation parameters among all patients. In patients with bilateral anterior disc displacement, the airway is narrow, the mandible retracts, and the hyoid bone is lower and more posterior. The linear ratio of condylar sagittal position （LR） was correlated with the airway volume of palatopharynx and glossopharynx, and with the length of the mandibular body and the sagittal position of mandible. Conclusions: Patients with bilateral anterior disc displacement and patients with OSAHS show similar changes in the position of mandible and hyoid bone. When meeting patients with joint problems, stomatologists should pay attention to asking them whether they have problems with airway obstruction.
	TO cite this article:Hou Yijun,Pan Xiaojing. Measurement and correlation analysis of upper airway and mandible, hyoid in patients with bilateral anterior disc displacement[OL].[23 March 2022] http://en.paper.edu.cn/en_releasepaper/content/4757016


	2. Investigation on the wear behavior of human tooth dentin treated by gamma irradiation
	Ping Qing,Shengbin Huang,Shanshan Gao,Linmao Qian,Haiyang Yu
	Clinical Medicine 08 December 2015
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	Abstract:This study aimed to evaluate the effect of gamma irradiation on the wear behavior of human tooth dentin in terms of alteration in crystallography and composition. Human premolars (n = 19) were collected to obtain perpendicular-sectioned dentin slides and parallel-sectioned dentin slides. Each slab was subjected to 2 Gy radiation dose to a cumulative dose of 60 Gy for 5 d a week, which lasted ix weeks. Nano-scratch tests were conducted on dentin surfaces with progressive loads from 0.1 mN to 80 mN. Scratch traces were observed using a scanning electron microscope and a surface profilometer. The effect of irradiation treatment on crystallography and chemical composition of dentin was investigated through X-ray diffraction and Fourier transform infrared spectroscopy. Changes in surface microhardness (SMH) were also evaluated. Nano-scratch results showed that the coefficient of friction of dentin increased after irradiation was administered; the depths and widths of the scratches were larger than those of dentin before irradiation was administered. Irradiation decreased the crystallinity of dentin and induced the formation of large crystals. The carbonate:mineral (C:M) ratio also increased. SMH significantly decreased after irradiation was administered. The main damage mechanisms involved delamination and crack formation in perpendicular-sectioned slides and parallel-sectioned slides of dentin after irradiation was administered. Therefore, irradiation directly affected the wear behavior of dentin on human tooth; irradiation also altered crystallography, chemical composition, and SMH of dentin.
	TO cite this article:Ping Qing,Shengbin Huang,Shanshan Gao, et al. Investigation on the wear behavior of human tooth dentin treated by gamma irradiation[OL].[ 8 December 2015] http://en.paper.edu.cn/en_releasepaper/content/4670360


	3. Expression and localization of Nell-1 during murine molar development
	Wang Xiaoying
	Clinical Medicine 28 December 2012
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	Abstract:Nel-like molecule-1 (Nell-1) is a recently discovered secreted protein that plays an important role in osteoblast differentiation, bone formation, and bone regeneration. However, its expression and distribution during tooth development are largely unknown. The aim of this study was to investigate the expression patterns of Nell-1 during murine molar development by immunohistochemistry. Nell-1 protein was expressed during molar development in embryonic and postnatal Kunming mice, but its expression levels and patterns at various developmental stages differed. At embryonic day 13.5 (E13.5) and E14.5, Nell-1 was found in both the entire enamel organ and the underlying mesenchyme. At E16.5, it was detected in the inner and outer enamel epithelia, stratum intermedium, secondary enamel knot, and dental papilla. At E18.5, Nell-1 was expressed in the differentiating ameloblasts, differentiating odontoblasts, and stratum intermedium. Positive staining was also found in the outer enamel epithelium. At postnatal day 2.5 (P2.5), P5, and P7, Nell-1 appeared in the secretory and mature ameloblasts and odontoblasts (odontoblastic bodies and processes) as well as immature enamel. Hertwig's epithelial root sheath also stained positively at P7. At P13.5, positive staining was restricted to the reduced dental epithelium and odontoblasts, whereas Nell-1 disappeared in the mature enamel. During tooth eruption, Nell-1 was observed only in the odontoblastic bodies, odontoblastic processes, and endothelial cells of blood vessels. The spatiotemporal expression patterns of Nell-1 during murine tooth development suggest that it might play an important role in ameloblast and odontoblast differentiation, secretion and mineralization of the extracellular enamel matrix, molar crown morphogenesis, as well as root formation.
	TO cite this article:Wang Xiaoying. Expression and localization of Nell-1 during murine molar development[J].


	4. Icariin: Can an herbal extract enhance dental implant outcomes?
	Wang Xiaoying
	Clinical Medicine 20 December 2012
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	Abstract:Introduction: The success rate of dental implant is closely related to three aspects including supporting bone before implantation, osseointegration process, and prevention of peri-implantitis after implantation. Icariin is one of the traditional Chinese herbal medicines to treat impotence, improve sexual function, and enhance immune function. Recently, there are abundant evidences showing that icariin can strengthen bones, enhance bone healing, inhibit osteopenia, and inhibit inflammation. The Hypothesis: We hypothesize that Icariin may be applied clinically to strengthen and accelerate osseointegration of dental implants, inhibit the peri-implantitis, and shorten the rehabilitation time. Evaluation of the Hypothesis: Limited success has been achieved to help implant surgery in spite of much effort. The application of icariin might be a potential method to improve the success rate of dental implant, contributing greatly to implant dentistry.
	TO cite this article:Wang Xiaoying. Icariin: Can an herbal extract enhance dental implant outcomes?[J].


	5. Fibroblast activity on multilayer laminin5 beta3 DNA coating titanium surface
	JIANG Qiaohong,ZHAO Shifang,GONG Xue,HE Fuming,YANG Guoli
	Clinical Medicine 02 April 2012
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	Abstract:This work was to investigate the fabrication of multilayer gene-functionalized films composed of cationic liposome (Lip) and pEGFP-C1-LAMB3 (pGL) DNA on titanium surfaces and the effect of the gene coatings on NIH3T3 cells. The multilayer gene coatings were fabricated on titanium by layer-by-layer (LbL) assembly technique. Surfaces analysis was carried out by contact angle measurements and X-ray photoelectron spectroscopy (XPS). Formation of LBL films was confirmed by fluorescence microscope. A biological study with NIH3T3 was followed over a 7-day culture period at days 0, 3 and 7 to observe the viability. This study showed polyelectrolyte multilayer films could be successfully deposited. EGFP expression increased with adding layer numbers. NIH3T3 adhered on multilayered DNA coatings displayed significantly higher (P<0.05) cell viability compared to control group both for 3 and 7 days culture. The results indicated the HA/pGL coatings improve the cytocompatibility of native titanium surface and presents a promising platform for delivery of DNA to NIH3T3 cells.
	TO cite this article:JIANG Qiaohong,ZHAO Shifang,GONG Xue, et al. Fibroblast activity on multilayer laminin5 beta3 DNA coating titanium surface[OL].[ 2 April 2012] http://en.paper.edu.cn/en_releasepaper/content/4473769


	6. Human Dedifferentiated Fat Cells Exhibit Perivascular Phenotype and Behaviors with Tissue-Specific Differentiation Ability
	Shen Jiefei,Sugawara Atsunori,Yamshita Joe,Sato Soh
	Clinical Medicine 03 March 2012
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	Abstract:Objectives: Dedifferentiated fat (DFAT) cells derived from mature adipocytes have been considered to be a homogeneous group of multipotent cells, which might be an alternative source of adult stem cells for regenerative medicine. However, many aspects of the cellular nature about DFAT cells remained unclarified. Based on recent understandings on the connection between the tissue-specific progenitor and perivascular cells, this study aimed to elucidate the basic characteristics of DFAT cells underlying their perivascular functions and differentiation potentials. Methods and Results: By modified ceiling culture technique, DFAT cells were converted from human adipocytes in the buccal fat pads. Flow cytometry analysis revealed that those derived cells were a homogeneous population of CD13+ CD29+ CD105+ CD44+ CD140b+ CD31- CD34- CD309- CD106- CD146- α-SMA- cells. Compared with previous reports, DFAT cells in this study demonstrated tissue-specific differentiation properties with strong adipogenic but much weaker osteogenic capacity. Neither did they exhibit endothelial phenotype under angiogenic induction. For the first time, we found human DFAT cells could undertake characteristics of perivascular lineage, including expression of pericyte markers, Dil-ac-LDL uptake ability, vascular network formation ability on Matgrigel. Furthermore, DFAT cells could largely promote and stabilize the vessels structures formed by human umbilical vascular endothelial cells (HUVECs) in vitro. Conclusions: This study provided novel evidence on the pericyte nature of human DFAT cells, which also supported the recent understanding about the perivascular origin of adult stem cells that tissue-specific progenitor cells in mesenchymal tissues would associate with the blood vessels, exhibiting perivascular characteristics and functions.
	TO cite this article:Shen Jiefei,Sugawara Atsunori,Yamshita Joe, et al. Human Dedifferentiated Fat Cells Exhibit Perivascular Phenotype and Behaviors with Tissue-Specific Differentiation Ability[OL].[ 3 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4464147

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