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1. An Electrochemical Sensor for Prothrombin-Time Test | |||
XIN Lifei,LIU Hong | |||
Chemistry 22 March 2018 | |||
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Abstract:As a new interdisciplinary, blood coagulation and thrombosis, being involved in the development and progression of many diseases, have a prominent clinical significance. Prothrombin time (PT time) is the time when calcium and tissue factor is added into the blood sample until when the blood sample completely clots. Clinically, PT time is an extremely sensitive screening index in coagulation system, it has an irreplaceable influence to patients\' daily medication and related treatments as it can primarily reflect the normality of the extrinsic coagulation. Until recently, most automated coagulation analyzers used in hospitals can detect a variety of samples and projects at the same time with high accuracy. However it could only be operated by the professionals, not to mention the high production and testing costs. For these reasons, the frequency and ease-of-operation of the detection have been limited. In order to develop a real-time and household detection method, paper-chips and POCT technology are mainly utilized in this work. Paper-chips possesses their own advantages, as it can easily reflect the blood clotting process by detecting a change in the conductance of blood during the blood coagulation process. After a series of design, tests and improvements, a chip for PT time testing is fabricated. Using this chip, the PT time of several blood samples are tested, and the testing results confirmed that the further improved chip can complete the measurement and correction of blood PT time test. Compared with conventional coagulation analyzer, paper-chips have several intrinsic advantages such as reduced cost, higher accuracy, and better application in POCT use. We believe that pushing the further experimental products to market could fill the vacancy in the domestic industry. | |||
TO cite this article:XIN Lifei,LIU Hong. An Electrochemical Sensor for Prothrombin-Time Test[OL].[22 March 2018] http://en.paper.edu.cn/en_releasepaper/content/4744019 |
2. Improvement of Loading Behavior of Myoglobin into Weak Polyelectrolyte Layer-by-Layer Films by Acid Treatment: Electrochemical and Electrocatalytical Studies | |||
Sun Peng,Hu Naifei,Liu Hongyun | |||
Chemistry 06 January 2011 | |||
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Abstract:The treatment of {PAH/PAA}n layer-by-layer films with pH 2.5 solutions has a great influence on loading of myoglobin (Mb) from its pH 5.0 buffers into the films, where PAH = poly(allylamine hydrochloride) and PAA = poly(acrylic acid). Without acid treatment, the {PAH/PAA}n films assembled on pyrolytic graphite electrodes are difficult to load or "absorb" Mb from its solution into the films, and the CV response of {PAH/PAA}n-Mb films is very small. However, after {PAH/PAA}n films are immersed in pH 2.5 buffers for only several minutes, the films can load much larger amounts of Mb from its solutions, and the corresponding {PAH/PAA}n-Mb films show good and nearly reversible cyclic voltammetric response with large peak currents. The direct electrochemistry of {PAH/PAA}n-Mb films can also be used to electrochemically catalyze the reduction of oxygen and hydrogen peroxide in solution. Electrochemical impedance spectroscopy and scanning electron microscopy results show that the permeability and porosity of {PAH/PAA}n films are significantly improved after the acid treatment, and thus beneficial for the loading of Mb from its solution. | |||
TO cite this article:Sun Peng,Hu Naifei,Liu Hongyun. Improvement of Loading Behavior of Myoglobin into Weak Polyelectrolyte Layer-by-Layer Films by Acid Treatment: Electrochemical and Electrocatalytical Studies[OL].[ 6 January 2011] http://en.paper.edu.cn/en_releasepaper/content/4404807 |
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