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1. Biocatalytic mechanism of monolignols biotransformation by Rhus laccases in water-miscible organic solutions | |||
Wan YunYang,Tetsuo Miyakoshi,Du Yumin,Chen Lvjun,Hao Jimin | |||
Biology 16 October 2009 | |||
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Abstract:Important monolignols such as coniferyl alcohol were catalyzed by Rhus laccases from Rhus vernicifera in water/acetone solution. The enzymatic mechanism was detailedly discussed. The sites 6, 円 and phenolic oxygen were the mainly active sites of phenylpropanoid compounds which were first oxidized by enzyme and then radical reaction followed. | |||
TO cite this article:Wan YunYang,Tetsuo Miyakoshi,Du Yumin, et al. Biocatalytic mechanism of monolignols biotransformation by Rhus laccases in water-miscible organic solutions[J]. |
2. Study on Endophytic fungi producing Orange Pigment isolated from Ginkgo Biloba L. | |||
Jia-Jia Liu,Shu-Juan Chen,Han-Xiang Gong | |||
Biology 07 August 2008 | |||
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Abstract:More than seventy endophytic fungi were isolated by aseptic techniques from the phloem of the root of Ginkgo biloba L.. They were cultured in the potato dextrose (PD) liquid medium and one endophytic fungus (Gh01) was proved can produce orange pigment. The orange pigment was identified as quercetin glycoside by chemical reaction and HPLC. This is the first report on quercetin glycoside produced by endophytic fungus. The effects of the carbon and nitrogen sources, metal ions, initial pH and incubation temperature on pigment production of the endophytic fungus were evaluated. The optimal temperature and initial pH for pigment production in PD liquid medium were 28℃ and 7.0, respectively. Through the orthogonal trial, 20 g/L Glucose and 5 g/L peptone were the most suitable carbon and nitrogen source, 1 g/L chloridize zinc could increase the yield of pigment. Under the optimal conditions established, the maximum yield of the pigment was 27.515 g/L after 120 hours’ successive culture. | |||
TO cite this article:Jia-Jia Liu,Shu-Juan Chen,Han-Xiang Gong. Study on Endophytic fungi producing Orange Pigment isolated from Ginkgo Biloba L.[OL].[ 7 August 2008] http://en.paper.edu.cn/en_releasepaper/content/23292 |
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