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| There are 60 papers published in subject: since this site started. | |||
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| 1. The total AvBD-6 gene expressed by Pichia pastoris | |||
| Wenyu Sun,Anshan Shan,Qingquan Ma,Na Dong,Yanping Cao,Liang Wang,Zeyun Wang,Changjiang Yan | |||
| Animal Husbandry, Veterinary Medicine 09 January 2013 | |||
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| Abstract:Avian β-defenisin 6 (AvBD-6) is a 67 amino acid-long peptide that plays an important role in the innate immunity of chickens and is widely expressed in most tissues. In this study, the codon-optimized AvBD-6 gene was synthesized and cloned into the eukaryotic expression vector pPICZαA and expressed in Pichia pastoris. Expression levels of the recombinant AvBD-6 were tested by Tricine-SDS-PAGE, and the product concentration reached 213.0 mg/l after optimization of the expression conditions. Recombinant AvBD-6 exhibited antimicrobial activity against bacteria with the minimal inhibition concentration (MIC) from 5.8 to 46.6 μg/ml and showed no hemolytic activity. | |||
| TO cite this article:Wenyu Sun,Anshan Shan,Qingquan Ma, et al. The total AvBD-6 gene expressed by Pichia pastoris[OL].[ 9 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4512514 | |||
| 2. Molecular phylogenetic studies on ascarid nematodes from Ailuropoda melanoleuca and seven other species of captive wild mammals based on ND1 genes | |||
| HE Guangzhi,NIU Lili,WANG Tao,WANG Qiang,CHEN Shijie,YAN Yubo,ZHANG Zhihe,YU Hua,DENG Jiabo,WANG Shuai,YAN Huijuan,YU Xinming,CHEN Weigang,FU Yan,GU Xiaobin,WANG Shuxian,PENG Xuerong,YANG Guangyou | |||
Animal Husbandry, Veterinary Medicine 28 December 2012
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| Abstract:In this paper, the mitochondrial gene NADH dehydrogenase subunit 1 (ND1) were used to determine the taxonomic status of ascarids from eight species of captive wild mammals (Ailuropoda melanoleuca, Ailurus fulgens,Ursus maritimus, Ursus arctos pruinosus, Ursus thibetanus mupinensis,Ursus arctos lasiotus,Pan troglodytes and Hylobates hoolock). Phylogenetic relationships were inferred by three methods (NJ/MP/ML) based on ND1 sequences. The alternative trees revealed that these roundworms were gathered together, suggested the ascarid nematodes from A. melanoleuca, A. fulgens and four species of bears belong to Baylisascaris,while worms from Pan troglodytes and Hylobates hoolock belong to Ascaris. The results supported the view that the roundworm from giant panda (Ailuropoda melanoleuca) was named as Baylisascaris schroederi, and proposed that roundworm from red panda (Ailurus fulgens) should be designated to Baylisascaris ailuri. | |||
| TO cite this article:HE Guangzhi,NIU Lili,WANG Tao, et al. Molecular phylogenetic studies on ascarid nematodes from Ailuropoda melanoleuca and seven other species of captive wild mammals based on ND1 genes[OL].[28 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4509210 | |||
| 3. Protection immunity of Toxoplasma gondii rhoptry protein 18 (ROP18) in mice | |||
| YUAN Ziguo,ZHANG Xiuxiang,LIN Ruiqing,ESKILD Petersen,ZHU Xingquan | |||
| Animal Husbandry, Veterinary Medicine 18 December 2012
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| Abstract:Toxoplasma gondii is an obligate intracellular protozoan parasite infecting a large variety of domestic and wild animals and human beings. Rhoptries are major players in T. gondii invasion and host cell interaction, and have been implicated as important virulence factors. In this study, we constructed a DNA vaccine expressing rhoptry protein 18 (ROP18) of T. gondii, and evaluated the immune response it induced in Kunming mice. The gene sequence encoding ROP18 was inserted into the eukaryotic expression vector pVAX I. We immunized Kunming mice intramuscularly. After immunization, we evaluated the immune response using lymphoproliferative assay, cytokine and antibody measurements, and the survival times of mice challenged lethally. The results showed that the group immunized with pVAX-ROP18 developed a high level of specific antibody responses against T. gondii ROP18 expressed in E. coli, a strong lymphoproliferative response, and significant levels of IFN-γ, IL-2, IL-4 and IL-10 production, compared with the other groups immunized with either empty plasmid or phosphate-buffered saline, respectively. These results demonstrate that pVAX-ROP18 induces significant humoral and cellular Th1 immune responses. After lethal challenge, the mice immunized with the pVAX-ROP18 showed a significantly increased survival time (27.9±15.1 days) compared with control mice which died within 7 days of challenge. Our data demonstrate, for the first time, that ROP18 triggered a strong humoral and cellular response against T. gondii, and that the antigen is a promising vaccine candidate against toxoplasmosis, worth further development. | |||
| TO cite this article:YUAN Ziguo,ZHANG Xiuxiang,LIN Ruiqing, et al. Protection immunity of Toxoplasma gondii rhoptry protein 18 (ROP18) in mice[OL].[18 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4506047 | |||
| 4. Analysis of Ghrelin and growth hormone secretagogue receptor expressions in mouse thymic epithelial cells | |||
| LIU Jianhong,MA Yongjiang,LI Ying,ZHANG Yuan,LI Yugu | |||
| Animal Husbandry, Veterinary Medicine 14 December 2012
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| Abstract:The present study was to identified the expression of Ghrelin and GHS-R in mouse thymic medullary-type epithelial cells (MTEC1).. Whether the gene of Ghrelin and GHS-R are expressing in MTEC1 cells or not was measured by using quantitative real time RT-PCR (Q-PCR). Results identified that the mRNA of Ghrelin and GHS-R can be detected in MTEC1 cells. The protein levels of GHS-R and ghrelin precursor were detected by the method of western blot analysis. Moreover, the location of GHS-R in MTEC1 cells were detected by immunofluorescence and the quantity of ghrelin in the supernatant of MTEC1 cells were measured by ELISA. The results of western blot analysis make sure that GHS-R and precursor of ghrelin are expressing in MTEC1 cells. GHS-R is a kind of transmembranes protein. as the results of immunofluorescence shown The quantity of ghrelin in the supernatant of MTEC1 cells measured by ELISA was 39.20 pg/ml. | |||
| TO cite this article:LIU Jianhong,MA Yongjiang,LI Ying, et al. Analysis of Ghrelin and growth hormone secretagogue receptor expressions in mouse thymic epithelial cells[OL].[14 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4504387 | |||
| 5. Changes in miRNAs expression profiling in testis of mice exposure to Sulfur Dioxide and Fluoride | |||
| ZHANG Jianhai,LIANG Chen,NIU Ruiyan,SUN Zilong,WANG Jundong | |||
| Animal Husbandry, Veterinary Medicine 12 December 2012
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| Abstract:MicroRNAs (miRNAs) are believed to play important roles in developmental and other cellular processes by hybridizing to complementary target mRNA transcripts, also involved in the development of mammalian spermatogenesis. Fluoride and sulfur dioxide are two well known environment pollutants, which present a serious threat to male reproduction. To compare the global miRNA expression profiling and regulation pattern in mouse testes between normal mouse and F or SO2-treat mice by using miRNA microarray technology, 144 male Kunming mice were divided randomly into four groups of thirty-six mice each. One group was left untreated as controls, and the other three groups were administered, respectively, for thirteen consecutive weeks, 150 mg NaF/L (45 mg F-/L) in their drinking water, SO2 in ambient air (26.2 mg/m3 SO2, 3hr/day), or were exposed to both NaF and SO2 together. After 15 weeks, testis totals RNA of the mouse model were gathered for miRNA microarray study. Simultaneity, reproductive capacity of mice, DNA contents distribution in testis cells, and bcl-2, bax, and p53 genes mRNA expression were detected to interpret the changed miRNA expression profiles. The results exhibited that MiR-106a in the NaF group, miR-449a, miR-16, miR-15b, and miR-34c in the SO2 groups, and miR-204 and miR-34a in the NaF+SO2 group were un-regulated significantly in testes of mice, compared with the controls, respectively. Whereas miR-34a decreased by 3.3-fold in the SO2 group, and miR-34b was down-regulated by more than 1.5-fold in the NaF+SO2 group. Further investigation indicates that predicted pathways according to significant miRNA are primary related to cell cycle, apoptosis, and MAPK signal pathway. Furthermore, the development of testis and reproductive capacity of male mouse have been weakened by F or SO2, especially, by F and SO2 together treatment. FCM analysis show that the percents of apoptosis cells in testis were increased significantly in experiment groups, DNA contents distributions have been disturbed by F or SO2. Expression of p53 and bcl-2 mRNA decreased, and bax gene was increased by1.87, 2.40 and 2.89 times as high as those in the control groups by QRT-PCR. However, both F or SO2 and the combination heighten the ratios of p53/bcl-2 and bax/bcl-2, all these changes lead to a common result of the spermatogenic cell apoptosis. In conclusion, changed miRNAs expression regulation should be look as the main mechanism of depressed male reproduction function of mouse induced by F or SO2 or their combination. This is the first miRNA microarray study to focus on evaluating altered miRNA expression profiles in testis of mice exposure to F or SO2. The miRNA microarray and bioinformatics analysis is a fast, high-throughout, exacts, foreground technique contribute toward elucidating the function of miRNAs in normal and impaired male reproduction by environment factors. | |||
| TO cite this article:ZHANG Jianhai,LIANG Chen,NIU Ruiyan, et al. Changes in miRNAs expression profiling in testis of mice exposure to Sulfur Dioxide and Fluoride[OL].[12 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4501514 | |||
| 6. Studies on Physiological Characteristics of Aging-resistence of Ultradried Ceratoides arborescens Seeds | |||
| TONG Lirong,DONG Kuanhu | |||
| Animal Husbandry, Veterinary Medicine 14 July 2012 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:Ceratoides arborescens seeds were tested for the effects of aging-resistance and free radical scavenging following seed ultradry. The results indicated that: (1)Ultradried seeds under the conditions of certain moisture content showed no adverse effect on seed vigor. Furthermore, ultradried seeds significantly enhanced aging-resistant capability under aging-accelerating condition (45℃, 2 days). The critical moisture content for Ceratoides arborescens seeds was about 3.78%. (2) Activities of the antioxidant enzymes including catalase and superoxide dismutase were reserved during storage in an ultradry state. (3) During ultradrying of Ceratoides arborescens seeds, the level of free radicals increased, but decreased as prehumidification before imbibition, which had a scavenging action on free radicals. | |||
| TO cite this article:TONG Lirong,DONG Kuanhu. Studies on Physiological Characteristics of Aging-resistence of Ultradried Ceratoides arborescens Seeds[OL].[14 July 2012] http://en.paper.edu.cn/en_releasepaper/content/4484927 | |||
| 7. Development of an indirect ELISA method for detecting antibody against Trichomonas gallinae from pigeon | |||
| Luo Fong,Su Suiqin,Liang Gang,Chen Zehua,LI Yawen,LI Guoqing | |||
Animal Husbandry, Veterinary Medicine 05 May 2012
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| Abstract:To develop an indirect ELISA method for detecting antibody of pigeon against T. gallinae， the optimal antigen density and positive serum dilution were determined by the chessboard test, then the specificity and reproducibility was detected, 72 positive and 45 negative serum were assayed with the indirect ELISA to assess the sensitivity and specificity and accuracy of the method. Results showed that the working concentration of antigen-antibody were optimal when the T. gallinae antigen was diluted to 5×105/mL and the positive serum was diluted to 1:400. The optimal incubation time of antigen-antibody reaction and antigen-antibody with anti-pigeon IgG:HRPO conjugate reaction was sixty minutes. The specificity, relative sensitivity, and accuracy were 86.67%, 100% and 94.87% respectively compared with wet film examination. The largest coefficient of variation of 10 serums was 11.22% in the repeatability test. The results indicate that the indirect ELISA method has good specificity, higher sensitivity and good repeatability. It is concluded that the indirect ELISA for detecting antibody against T. gallinae of pigeon was developed successfully. . | |||
| TO cite this article:Luo Fong,Su Suiqin,Liang Gang, et al. Development of an indirect ELISA method for detecting antibody against Trichomonas gallinae from pigeon[OL].[ 5 May 2012] http://en.paper.edu.cn/en_releasepaper/content/4477128 | |||
| 8. Zinc Prevents Salmonella enterica Serovar Typhimurium-Induced Loss of Intestinal Mucosal Barrier Function in broiler chickens | |||
| Zhang Bingkun,Shao Yuxin,Guo Yuming,Yuan Jianmin | |||
Animal Husbandry, Veterinary Medicine 26 April 2012
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| Abstract:The present study was carried out to evaluate the beneficial effects of supplemental zinc (Zn) on the intestinal mucosal barrier function in Salmonella enterica Serovar Typhimurium-challenged broiler chickens in a 42-d experiment. A total of 336 1-day-old male Arbor Acres broiler chicks were assigned to 8 treatment groups. A 4 × 2 factorial arrangement of treatments was used in a completely randomized experimental design to study the effects of levels of supplemental Zn (0, 40, 80, 120 mg/kg of diet), pathogen challenge (with or without S. Typhimurium challenge), and their interactions. S. Typhimurium infection caused reduction of growth performance (P <0.05) and intestinal injury as determined by reduced (P <0.05) villus height/crypt depth ratio and sucrase activity in the ileum, increased (P <0.05) plasma endotoxin levels, and reduced (P <0.05) claudin-1, occludin and MUC2 mRNA expression in the ileum at day 21. Zinc pretreatment tended to improve body weight gain (P = 0.072) in the starter period, increase the activity of ileal sucrase (P=0.077), but tended to reduce plasma endotoxin levels (P=0.080), and significantly increased (P <0.05) villus height/crypt depth ratio and mRNA levels of occludin and claudin-1 in the ileum at day 21. The results indicated that dietary Zn supplementation appeared to alleviate the loss of intestinal mucosal barrier function induced by S. Typhimurium challenge and the partial mechanism might be related to the increased expression of occludin and claudin-1 in broiler chickens. | |||
| TO cite this article:Zhang Bingkun,Shao Yuxin,Guo Yuming, et al. Zinc Prevents Salmonella enterica Serovar Typhimurium-Induced Loss of Intestinal Mucosal Barrier Function in broiler chickens[J]. | |||
| 9. Expression profiles of key candidate genes involved in steroidogenesis during follicular atresia in the pig ovary | |||
| Zengxiang Pan,Jinbi Zhang,Fei Lin,Xueshan Ma,Xuguang Wang,honglin Liu | |||
Animal Husbandry, Veterinary Medicine 20 April 2012
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| Abstract:More than 99% of follicles in mammalian ovaries undergo a degenerative process known as atresia, and thus only a limited number of ovarian follicles actually ovulate after full growth and development. The endocrinological regulatory mechanisms involved in follicular development have been studied extensively, but the precise and systematic molecular mechanisms of steroidogenesis enzymes involved in atresia are unclear. In the present study, we examined whether and how the steroidogenesis enzymes are involved in porcine ovary follicular atresia. Expression of steroidogenic acute regulatory protein, CYP11, CYP17, 3β-hydroxysteroid dehydrogenase (3β-HSD), CYP19, as well as related pituitary and ovarian hormone receptors were quantified in ovaries. During porcine follicular atresia, expressions of P450 cholesterol side chain cleavage enzyme, progesterone and androgen receptors increased significantly during the late atretic stage, while the expression of aromatase and follicle-stimulating hormone receptors decreased significantly in the early stage. These data suggested that the regulation of aromatase by follicle-stimulating hormone might induce follicular atresia, and that progesterone and androgen production further promoted follicular atresia. Additionally, a correlation analysis indicated a large and complex interactive network among these genes and the endocrinological microenvironment of the follicles. Significant correlations were observed between expression of steroidogenic enzymes and their receptors, and also between progesterone and 17β-estradiol (E2) levels in follicular fluid. Taken together, these results suggest that CYP19 plays a role during early atresia by regulating the production of E2, whereas CYP11 and 3β-HSD increase atresia progression by increasing progesterone levels. | |||
| TO cite this article:Zengxiang Pan,Jinbi Zhang,Fei Lin, et al. Expression profiles of key candidate genes involved in steroidogenesis during follicular atresia in the pig ovary[OL].[20 April 2012] http://en.paper.edu.cn/en_releasepaper/content/4476052 | |||
| 10. Molecular cloning, characterization and inhibitory effect on B-cell survival of BAFF-R, a novel receptor of TNFR family in horse (Equus caballus) | |||
| WU Haitao,KE Zhen,ZHANG Jianrong,XU Xinzhou,LIU Meng,ZHANG Shuangquan | |||
| Animal Husbandry, Veterinary Medicine 07 February 2012
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| Abstract:B-cell survival depends on signals induced by B-cell activating factor (BAFF) binding to its receptor (BAFF-R). In mice, mutations in BAFF or BAFF-R cause B-cell lymphopenia and antibody deficiency. However, the information about horse is poorly understood. Here, We report the identification and analysis of a novel horse TNF receptor related to B-cell suivival, BAFF-R. The horse BAFF-R gene spans 1444 bp, including three exons and located on chromosome 28. The horse BAFF-R protein is composed of 183 aa, with the calculated molecular weight 19 kDa and isoelectric point 6.12. As a type Ⅲ transmembrane protein, its extracellular domain has one cysteine-rich domain (CRD) containing four cysteine residues, and cytoplasmic domain exists the TRAF3 binding domain. The genetic relationship between horse and dog is the closest by the phylogenetic analysis of BAFF-R amino acids. Moreover, recombinant BAFF-R-Fc protein not only binds to B-cell, but also inhibits B-cell survival induced by BAFF in vitro. These studies provide the basis for investigation on its roles in regulating B lymphocyte survival and related immune diseases in horse. | |||
| TO cite this article:WU Haitao,KE Zhen,ZHANG Jianrong, et al. Molecular cloning, characterization and inhibitory effect on B-cell survival of BAFF-R, a novel receptor of TNFR family in horse (Equus caballus)[OL].[ 7 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4465365 | |||
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