Abstract:Hydrolysates A and P were derived from β-Conglycinin hydrolyzed by Alcalse and Pronase respectively, and then analyzed by gel chromatography of Sephadex G-25. Chromatography patterns showed that carbohydrates were mainly existed in fractions A1 of hydrolysate A and P1 of hydrolysate P, with carbohydrate content of 8.23% and 9.66% respectively, much higher than other fractions. The results of thin-layer chromatography for fractions A1 and P1 confirmed that the two fractions were carbohydrate-binding peptides, referred as CBP-A and CBP-P respectively. Two non-carbohydrate peptides A (NCP-A) and P (NCP-P) were derived from CBP-A and CBP-P treated with glycosidase F respectively. Hydrolysates A and P and fractions from them were observed inhibitory effect on mitogen-induced proliferation of murine splenocytes. Compared to NCP-A and A2, CBP-A had lower inhibitory effect at concentration of 0.5~5μg/ml, but stronger inhibitory effect at concentration of 500~100μg/ml. CBP-P had stronger inhibitory effect on mitogen-induced proliferation of splenocytes at all concentration of 0.5~1000μg/ml except at 50μg/ml. These results indicated that glycopeptides could be isolated from soy peptides. And inhibitory effects on mitogen-induced proliferation of splenocytes were influenced by carbohydrate chain.

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	1. Isolation and Identification of Glycopeptides from β-Conglycinin hydralysates and their effects on Proliferation of Mice Splenocytes
	Xue mei Zhang,Shun tang Guo,Li Yinghui,Song Jinping
	Food Science and Technology 08 February 2010
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	Abstract:Hydrolysates A and P were derived from β-Conglycinin hydrolyzed by Alcalse and Pronase respectively, and then analyzed by gel chromatography of Sephadex G-25. Chromatography patterns showed that carbohydrates were mainly existed in fractions A1 of hydrolysate A and P1 of hydrolysate P, with carbohydrate content of 8.23% and 9.66% respectively, much higher than other fractions. The results of thin-layer chromatography for fractions A1 and P1 confirmed that the two fractions were carbohydrate-binding peptides, referred as CBP-A and CBP-P respectively. Two non-carbohydrate peptides A (NCP-A) and P (NCP-P) were derived from CBP-A and CBP-P treated with glycosidase F respectively. Hydrolysates A and P and fractions from them were observed inhibitory effect on mitogen-induced proliferation of murine splenocytes. Compared to NCP-A and A2, CBP-A had lower inhibitory effect at concentration of 0.5~5μg/ml, but stronger inhibitory effect at concentration of 500~100μg/ml. CBP-P had stronger inhibitory effect on mitogen-induced proliferation of splenocytes at all concentration of 0.5~1000μg/ml except at 50μg/ml. These results indicated that glycopeptides could be isolated from soy peptides. And inhibitory effects on mitogen-induced proliferation of splenocytes were influenced by carbohydrate chain.
	TO cite this article:Xue mei Zhang,Shun tang Guo,Li Yinghui, et al. Isolation and Identification of Glycopeptides from β-Conglycinin hydralysates and their effects on Proliferation of Mice Splenocytes[OL].[ 8 February 2010] http://en.paper.edu.cn/en_releasepaper/content/40056


	2. Hydrothermal treatment followed by microbial transglutaminase reaction improved the water absorption of wheat gluten
	Song Liu,Dongxu Zhang,Long Liu,Miao Wang,Guocheng Du,Jian Chen
	Food Science and Technology 18 December 2009
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	Abstract:BACKGROUND: The water absorption of wheat gluten plays an important role in the weight, volume and form ratio of the breads. In this paper, hydrothermal treatment and microbial transglutaminase (MTGase) modification were combined to improve the water absorption ratio (WAR) of wheat gluten. To understand the increases in WAR, the changes in MTGase reaction after gluten hydrothermal treatment were also investigated. RESULTS: The sole hydrothermal treatment improved the WAR of gluten. The gluten treated at 100 oC for 30 min exhibited the highest WAR value (2.03g g-1 gluten) while the WAR of control without hydrothermal treatment was 1.5 g g-1 gluten. When gluten was exposed to 90 oC for 30 min followed by incubation with MTGase for 5 h, its WAR reached 2.48g g-1 gluten. In contrast to control gluten, the surface hydrophobicity of the gluten preheated at 90 oC for 30min increased and fluctuated in a different way during the following MTGase reaction. Meantime, the trend in the amount of soluble protein of preheated gluten was also changed in the progress of MTGase reaction. CONCLUSION: Hydrothermal treatment followed by MTGase reaction is an efficient approach to improve the WAR of wheat gluten. The analysis of catalytic process, including determination of ammonia, gluten surface hydrophobicity, soluble protein and SDS-PAGE, suggested that hydrothermal pretreatment accelerated the cross-linking reaction and may alter the ratio of gluten deamidation catalyzed by MTGase, which induced an increase in the WAR.
	TO cite this article:Song Liu,Dongxu Zhang,Long Liu, et al. Hydrothermal treatment followed by microbial transglutaminase reaction improved the water absorption of wheat gluten[OL].[18 December 2009] http://en.paper.edu.cn/en_releasepaper/content/37782

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	3. Study on Storage Quality of Chicken Soup Cooked by Earthen Jar and High Pressure
	Wan Ronge,Hu Jian,Xiong Shanbai,Zhao Siming
	Food Science and Technology 08 September 2009
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	Abstract:Chicken soup cooked by earthen jar and high pressure was stored at 4℃, respectively. During storage, the protein content, free amino acid content, protein digestibility, fat content, water soluble solid content of the chicken soup in muscle and soup was analyzed respectively. Chicken soup cooked by earthen jar was superior to that by high pressure in nutrition because of its higher protein digestibility. But the changes of nutrition component contents of the soup cooked by high pressure were fewer and the total number of collies was lower than that cooked by earthen jar, suggested better storage quality of high pressure cooking. Nutrition component content of chicken soup decreased except the crude fat content in muscle during storage, suggested lots biochemical changes in the chicken soup was caused. The law of the change of the parameters during storage fit first-order chemical reaction dynamics model. The reaction rate constant of the total number of collies was the largest, which could regard as an index to forecast and control the storage quality of chicken soup.
	TO cite this article:Wan Ronge,Hu Jian,Xiong Shanbai, et al. Study on Storage Quality of Chicken Soup Cooked by Earthen Jar and High Pressure[OL].[ 8 September 2009] http://en.paper.edu.cn/en_releasepaper/content/35017


	4. Transmembrane Behavior of Ca2+ in E. coli Cells impacted by High-Intensity Pulsed Magnetic Field
	Ma Haile,He Ronghai ,Wang Heli ,Luo Lin ,Wang Zhenbin
	Food Science and Technology 16 February 2009
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	Abstract:Transmembrane behavior of Ca2+ in E. coli cells impacted by high-intensity pulsed magnetic field (PMF) was studied. The bacteria were subjected to a treatment of PMF (0-4.5 T, 0-30 pulse numbers). The study found that intracellular calcium concentration ([Ca2+]i) of E. coli was changed significantly with PMF treatment. When sample was treated by PMF (2.5 T, 20 pulse numbers) at 30oC, total number colonies of E. coli 8099 sample decreased 63% while the [Ca2+]i increased 2.2 times as much as the sample without treatment. Change of [Ca2+]i in E. coli cells at logarithm phase is more sensitive to PMF treatment than those at other growth phases. There is certain relativity between transmembrane behavior of Ca2+ in E. coli cells and “biological window effect” corresponding to PMF sterilization effects, so the biological window effect of high-intensity PMF sterilization was validated preliminarily by transmembrane behavior of E. coli cell Ca2+. Effect of pulse number on [Ca2+]i is more placid than that of power. The increase of [Ca2+]i is an important reason of E. coli cells death in PMF sterilization.
	TO cite this article:Ma Haile,He Ronghai ,Wang Heli , et al. Transmembrane Behavior of Ca2+ in E. coli Cells impacted by High-Intensity Pulsed Magnetic Field[OL].[16 February 2009] http://en.paper.edu.cn/en_releasepaper/content/29059


	5. Biological Window Effects of High-intensity Pulsed Magnetic Field Sterilization on Escherichia Coli
	Ma Haile ,He Ronghai,Luo Lin ,Wang Zhenbin ,Wang Heli
	Food Science and Technology 16 February 2009
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	Abstract:High-intensity Pulsed Magnetic Field (PMF) sterilization is a promising technology for the nonthermal sterilization of food. A self-designed pulsed magnetic field sterilization equipment, whose power is up to 4.5 Tesla, was employed to investigate the inactivation effect of high-intensity PMF on Escherichia coli 8099 and to explore the biological effect of PMF sterilization. The survival rate (SR) of E. coli 8099 significantly decreased after PMF treatment. E. coli 8099 in log phase period is more sensitive to PMF treatment. Biological window effect of PMF power was found in high-intensity PMF sterilization of E. coli 8099. 1.5T, 2.5T, and 4T are a series of “power windows” and power windows consistent with the computative equation T = 0.25n (n+1) +1 (n is ordinal number of power window, n=1 to 3).
	TO cite this article:Ma Haile ,He Ronghai,Luo Lin , et al. Biological Window Effects of High-intensity Pulsed Magnetic Field Sterilization on Escherichia Coli[OL].[16 February 2009] http://en.paper.edu.cn/en_releasepaper/content/29044


	6. Cloning and expression of a bactericin sakacin P from Lactobacillus sakei in Escherichia coli
	Shuo Li,Haiqin Chen ,Fengwei Tian, ,Hao Zhang,Wei Chen
	Food Science and Technology 15 October 2008
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	Abstract:Sakacin P, a novel bactericin from Lactobacillus sakei, is a potential antimicrobial substrate, which has strong activity against food-borne pathogen eg. Listeria monocytogenes. In Lactobacillus sakei, the gene (sppA) of sakacin P was controled by strict regulation mechnism, so that the quantity of secreted sakacin P was low. In this study, we synthesized the sppA gene using splicing by overlap extension PCR (SOE-PCR), and cloned it into E. coli expression system. After the induction of IPTG, the recombinant sakacin P was successfully expressed. The collected cells were sonicated and the activity was identified by inhibition zone method using the cell-free supernatant. The results also showed that the low temperature introduction took more active sakacin P which has strong antimicrobial activity.
	TO cite this article:Shuo Li,Haiqin Chen ,Fengwei Tian, , et al. Cloning and expression of a bactericin sakacin P from Lactobacillus sakei in Escherichia coli[OL].[15 October 2008] http://en.paper.edu.cn/en_releasepaper/content/24827


	7. Application of response surface methodology for extraction optimization of the water-soluble polysaccharide from Pteridium aquilinum
	XU Wen-Tao,ZHANG Fang-Fang,Huang Kunlun,Wang Ying,Luo Yunbo
	Food Science and Technology 08 April 2008
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	Abstract:The optimum conditions for the extraction of the water-soluble polysaccharide from Pteridium aquilinum were determined using response surface methodology (RSM). A central-composite experimented design (CCD) was used to investigate the effects of three independent variables, namely liquid: solid ratio, extraction temperature and extraction time. Also, the antioxidative activity of the crude polysaccharide obtained from Pteridium aquilinum was investigated in this study. The optimum conditions were determined as a liquid: solid ratio of 18.8:1 (v/w), an extraction temperature of 62.5 ℃ and an extraction time of 5.9h. The crude polysaccharide showed better antioxidiative activity by FARP antioxidative assay.
	TO cite this article:XU Wen-Tao,ZHANG Fang-Fang,Huang Kunlun, et al. Application of response surface methodology for extraction optimization of the water-soluble polysaccharide from Pteridium aquilinum[OL].[ 8 April 2008] http://en.paper.edu.cn/en_releasepaper/content/20202


	8. Biocontrol of Postharvest Fungal Pathogens by Yeasts Isolated from Fruits and Marine Source
	Wang Yifei ,Feng Fengqin,Zheng Xiaodong ,Tang Haiqing
	Food Science and Technology 24 December 2007
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	Abstract:Fruit wounds and marine resources were utilized to screen for potential antagonists to postharvest rot of cherry tomato. Biocontrol activity of a total of 34 yeasts isolates were tested, among which four isolates were selected with the best efficacy in reducing the decay rate of Alternaria Alternata and Botrytis cinerea. One of the marine yeast isolated from East China Sea was identified as Rhodosporidium paludigenum Fell & Tallman, which has not been described previously as a biocontrol agent. The application of R. paludigenum is also effective for other postharvest rot of fruits. Its efficacy declined to a 36% reduction in pear, a 33% reduction in persimmon and an 69% reduction in Chinese jujube after one week of storage.
	TO cite this article:Wang Yifei ,Feng Fengqin,Zheng Xiaodong , et al. Biocontrol of Postharvest Fungal Pathogens by Yeasts Isolated from Fruits and Marine Source[OL].[24 December 2007] http://en.paper.edu.cn/en_releasepaper/content/17319


	9. Biocontrol of Alternaria alternata on Cherry Tomato Fruit by Use of marine yeast Rhodosporidium paludigenum Fell & Tallman
	Wang Yifei ,Feng Wu,Shen Danhong,Yu Ting ,Zheng Xiaodong
	Food Science and Technology 24 December 2007
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	Abstract:The vasidiomycetous yeast Rhodosporidium paludigenum Fell & Tallman, which was isolated from the south of East China Sea was evaluated for its activity in reducing postharvest decay of cherry tomatoes caused by Alternaria alternata in vitro and in vivo test. The result showed that washed cell suspensions of R. paludigenum provided better control of A. alternata than other treatments. Autoclaved cell culture failed to provide protection against the pathogen. The concentration of antagonist had significant effect on biocontrol effectiveness. When the washed yeast cell suspension reached the concentration of 1×109 CFU/ml and challenged with pathogen spore suspension at 5×104 spores/ml, the rate of decay was only 37% after 5-day incubation at 25℃. Efficacy of R. paludigenum was obvious when it was applied simultaneously or prior to inoculation with A. alternata. There was little efficacy when R. paludigenum was applied after A. alternata inoculation. The longer incubated time of R. paludigenum was, the lower disease incidence would be. When the incubation time was reached 54 h, the decay was only 33%. On the wounds of cherry tomato, the population of R. paludigenum increased 50-fold during the first 12 h at 25℃. The maximum marine yeast population was nearly 100-fold over the original ones after 24 h.
	TO cite this article:Wang Yifei ,Feng Wu,Shen Danhong, et al. Biocontrol of Alternaria alternata on Cherry Tomato Fruit by Use of marine yeast Rhodosporidium paludigenum Fell & Tallman[OL].[24 December 2007] http://en.paper.edu.cn/en_releasepaper/content/17308


	10. The effect of silencing of LeEIN2 gene in tomato fruit development and ripening
	HongLiang Zhu,Ben Zhong Zhu,Yi Shao,Da-Qi Fu,Xiao-Guang Wang,Xi-Jin Lin,Yuan-Hong Xie,Yun-Bo Luo
	Food Science and Technology 09 December 2005
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	Abstract:Loss-of-function ETHYLENE INSENSITIVE2 (EIN2) mutations showed ethylene insensitivity in Arabidopsis, which indicated an essential role of EIN2 in ethylene signaling. Recently, the solanaceae EIN2 (petunia EIN2) has also been identified. However, little is known about the role of EIN2 in other solanaceae species such as tomato. To gain a better understanding of EIN2 in tomato, LeEIN2 silenced tomato fruits were developed using a virus-induced gene silencing fruit system to study the role of LeEIN2 in tomato fruit ripening. Silenced fruits had a delay in fruit development and ripening, related to greatly descended expression of ethylene-related and ripening-related genes in comparison with those of control fruits. Therefore, LeEIN2 was proposed to a positive component of the ethylene-signaling pathway in tomato. In addition, there were less seeds and locules in the silenced fruit than the control fruit, like the phenotype of parthenocarpic tomato fruit. The content of auxin and also the expression of auxin-regulated gene were declined in silenced fruit, which indicated the EIN2 might be important for the crosstalk between ethylene and auxin hormones.
	TO cite this article:HongLiang Zhu,Ben Zhong Zhu,Yi Shao, et al. The effect of silencing of LeEIN2 gene in tomato fruit development and ripening[OL].[ 9 December 2005] http://en.paper.edu.cn/en_releasepaper/content/4202

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