Abstract:Among the tested Lactobacillus strains assayed for Inhibitory activity on monoamine oxidase (MAO), the supernatant of Lactobacillus casei JH23 cultured in MRS broth showed strong inhibitory activity. The bioactive components from the supernatant could be fractioned into organic solvent soluble (O) and water soluble ones (W) by extraction with ethyl acetate. Both the organic solvent soluble (O) and water soluble components (W) demonstrated a competitive inhibitory activity to MAO in a dose-dependent manner. The IC50 for the organic solvent soluble components (O) to MAO-A and MAO-B were 1.97mg / ml, 5.67mg / ml, while IC50 for the water soluble components (W) were 1.59mg / ml and 4.83mg / ml respectively. A bioactive fraction w1 was obtained from the water soluble components (W) by sephadex-G15 chromatograph, which the IC50 for MAO-A and MAO-B were 0.327mg/ml and1.13mg/ml .W1 could be further fractioned into 2 peaks with absorbance at 200nm and 300nm respectively by RP-HPLC, and the peak appeared at 200nm had good MAO inhibitory effect. The substance will be prepared by liquid phase and determined structure, which can promote the development of spiritual degeneration disease drug.

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	1. Inhibitory Effect on Monoamine oxidase of Lactobacillus casei JH23's Metabolite
	HE Yiwei,GUO Benheng,CHEN Wei,WU Zhenjun,WU Jiang
	Food Science and Technology 20 March 2012
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	Abstract:Among the tested Lactobacillus strains assayed for Inhibitory activity on monoamine oxidase (MAO), the supernatant of Lactobacillus casei JH23 cultured in MRS broth showed strong inhibitory activity. The bioactive components from the supernatant could be fractioned into organic solvent soluble (O) and water soluble ones (W) by extraction with ethyl acetate. Both the organic solvent soluble (O) and water soluble components (W) demonstrated a competitive inhibitory activity to MAO in a dose-dependent manner. The IC50 for the organic solvent soluble components (O) to MAO-A and MAO-B were 1.97mg / ml, 5.67mg / ml, while IC50 for the water soluble components (W) were 1.59mg / ml and 4.83mg / ml respectively. A bioactive fraction w1 was obtained from the water soluble components (W) by sephadex-G15 chromatograph, which the IC50 for MAO-A and MAO-B were 0.327mg/ml and1.13mg/ml .W1 could be further fractioned into 2 peaks with absorbance at 200nm and 300nm respectively by RP-HPLC, and the peak appeared at 200nm had good MAO inhibitory effect. The substance will be prepared by liquid phase and determined structure, which can promote the development of spiritual degeneration disease drug.
	TO cite this article:HE Yiwei,GUO Benheng,CHEN Wei, et al. Inhibitory Effect on Monoamine oxidase of Lactobacillus casei JH23's Metabolite[OL].[20 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4472390


	2. In vitro screening of lactobacilli with antagonistic activity against Campylobacter jejuni growth and infection in human intestinal epithelial cells
	Yu Zhao,Fengwei Tian,Haiqin Chen,Xiaoming Liu,Gang Wang,Qiuxiang Zhang,Jianxin Zhao,Hao Zhang,Wei Chen
	Food Science and Technology 19 March 2012
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	Abstract:Campylobacter jejuni is identified as the leading bacterial pathogen causing enterocolitis in human, usually leading to watery or bloody diarrhea, abdominal pain. Previous studies reported that some lactic acid bacteria have inhibitory effects on pathogenic bacteria, however the protective effects of lactobacilli strains on infection with C.jejuni have not been extensively studied. In this study, seventy-eight lactobacilli strains were screened for anti-C.jejuni activity using well diffusion agar assays and the ability of adhesion to human intestinal epithelial (HT-29) cell were also assessed. Four lactobacilli strains, i.e, strain N8, N9, ZL4 and ZL5, showed obvious potential anti-C.jejuni growth in vitro as well as their significant ability of adhesion to the HT-29 cell. Furthermore, these four lactobacilli strains showed significant potential for reducing adhesion and invasion of C.jejuni to HT-29 cell in exclusion, competition and displacement assays, respectively. All the four strains also performanced a desired resistance to the simulated gastrointestinal conditions. Taken together, this study suggests that lactobacilli strains, N8, N9, ZL4 and ZL5 could be used as potential probiotics for prevention infection with C.jejuni .
	TO cite this article:Yu Zhao,Fengwei Tian,Haiqin Chen, et al. In vitro screening of lactobacilli with antagonistic activity against Campylobacter jejuni growth and infection in human intestinal epithelial cells[OL].[19 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4472323

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	3. Lactobacillus rhamnosus CCFM 1107 treatment ameliorates alcohol-induced liver injury in a mouse model of chronic alcohol feeding
	CHI Feifei,TIAN Fengwei,WANG Gang,LIU Xiaoming,ZHANG Qiuxiang,ZHANG Hao,CHEN Wei
	Food Science and Technology 17 March 2012
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	Abstract:Lactobacillus rhamnosus CCFM 1107 was screened for high antioxidative activity from over 50 lactobacilli. The present study attempted to explore the in vivo protective properties of Lactobacillus rhamnosus CCFM1107 in alcoholic liver injury. A mouse model was induced by orally feeding alcohol by gavage when simultaneously treated with Lactobacillus rhamnosus CCFM 1107, the drug Hu-Gan-Pian, Lactobacillus rhamnosus GG, and Lactobacillus plantarum CCFM 1112 for 3 months. Biochemical analysis was performed for both serum and liver homogenate. Detailed intestinal flora and histological analyses were also carried out. Our results indicated that the administration of Lactobacillus rhamnosus CCFM 1107 signi?cantly inhibited the increase in the levels of serum aminotransferase and endotoxin, as well as the content of triglycercide and cholesterol in the serum and in the liver. Glutathione, glutathione peroxidase and superoxide dismutase were elevated while the levels of malondialdehyde were decreased. The enteric dysbiosis caused by alcohol was restored by increasing the numbers of both lactobacilli and bifidobacteria and decreasing the numbers of both enterococci and enterobacter. Histological analysis confirmed the protective effect of Lactobacillus rhamnosus CCFM 1107. In contrast to the other lactobacilli and to the drug administered in the study, we conclude that Lactobacillus rhamnosus CCFM 1107 provides a protective effect on alcoholic liver injury by reducing oxidative stress and restoring the intestinal flora.
	TO cite this article:CHI Feifei,TIAN Fengwei,WANG Gang, et al. Lactobacillus rhamnosus CCFM 1107 treatment ameliorates alcohol-induced liver injury in a mouse model of chronic alcohol feeding[OL].[17 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4472100


	4. Screening of lactobacilli with antagonistic activity against enteroinvasive Escherichia coli
	Wenyu Liu,Xiaoming Liu,Fengwei Tian,Qiuxiang Zhang,Gang Wang,Hao Zhang,Wei Chen
	Food Science and Technology 15 March 2012
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	Abstract:In the present study, 339 lactic acid bacteria (LAB) strains, isolated from fermented dough, pickles, salted meat, baby feces and fermented dairy products, were screened for the antagonistic activity against enteroinvasive Escherichia coli (EIEC). Through the analysis of the inhibition zone of the lactobacilli spent culture supernatant (SCS) on EIEC growth, 22 LAB strains were selected. Using the in vitro assays of adhesion to HT-29 cell lines and tolerance to gastrointestinal conditions (acid and bile), two isolates, Lactobacillus plantarum CCFM 233 and Lactobacillus plantarum CCFM 231, were shown not only to inhibit the growth of EIEC significantly, but also to have high adhesion to HT-29 cell lines and good tolerance to low acid and high bile. Furthermore, the two strains could strongly antagonize the adhesion and invasion of EIEC to the HT-29 cell lines. This study suggests that Lactobacillus plantarum CCFM 233 and Lactobacillus plantarum CCFM 231 could be used as potential probiotics against EIEC.
	TO cite this article:Wenyu Liu,Xiaoming Liu,Fengwei Tian, et al. Screening of lactobacilli with antagonistic activity against enteroinvasive Escherichia coli[OL].[15 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4471789


	5. A Comparison Study Of Three Nonlinear Multivariate Data Analysis Methods For Smartongue: Kernel PCA, LLE and Sammon mapping
	Tian Shiyi,Chen Min,Deng Shaoping
	Food Science and Technology 26 January 2012
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	Abstract:Smartongue was a voltammetric electronic tongue developed by the laboratory, which was based on multifrequency large amplitude pulse voltammetry (MLAPV) and a non-specific sensors array of six naked metallic electrodes. Three non-linear multivariate data analysis methods, such as Kernel principal component analysis (Kernel PCA), Locally linear Embedding (LLE) and Sammon mapping were studied about the data processing ability for Smartongue. Normal principal component analysis (PCA) was used as a reference method and discrimination index (DI value) was used as a quantitative indicator for evaluation the separation ability of different plot. The result indicated that non-linear data processing methods had a more powerful ability than PCA for processing the data of Smartongue. Among these techniques, Sammon mapping successfully classified three bitter solutions, six artificial green tea products and five milk power solutions with different storage time well and exhibited the best data processing ability for extracting the useful information from the data of Smartongue.
	TO cite this article:Tian Shiyi,Chen Min,Deng Shaoping. A Comparison Study Of Three Nonlinear Multivariate Data Analysis Methods For Smartongue: Kernel PCA, LLE and Sammon mapping[OL].[26 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4463710


	6. Sinusoid envelope voltammetry - A novel voltammetry waveform for electronic tongue
	Tian Shiyi,Xiao Xiao,Deng Shaoping
	Food Science and Technology 22 January 2012
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	Abstract:A novel voltammetry for voltammetric electronic tongue so called sinusoid envelope voltammetry was introduced in this paper. Fourier transformation was used to transform the data of its response signal from the time domain to the frequency domain. Four taste substances, i.e. acesulfame potassium, monosodium glutamate, potassium chloride and tartaric acid, exhibited abundant frequency characters in the power spectrum of Fourier transformation respectively, which indicated that the power spectrum from sinusoid envelope voltammetry could be used as fingerprints of samples for classification. Also, principal component analysis with discrimination index and multi-frequency large amplitude pulse voltammetry as a reference technique was used to evaluate the separation ability of sinusoid envelope voltammetry. The score plot of sinusoidal envelope voltammetry from four taste substances and five brands of Jiafan rice wine showed a better discrimination ability than multi-frequency large amplitude pulse voltammetry. Sinusoid envelope voltammetry would be a promising technology for voltammetric electronic tongue.
	TO cite this article:Tian Shiyi,Xiao Xiao,Deng Shaoping. Sinusoid envelope voltammetry - A novel voltammetry waveform for electronic tongue[OL].[22 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4463707


	7. Postharvest control of citrus green mold by the integration of sodium bicarbonate and Rhodosporidium paludigenum
	Zhu Ruiyu,Yu Ting,Zheng Xiaodong
	Food Science and Technology 14 November 2011
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	Abstract:The object of this study was to analyze the impact of the marine yeast Rhodosporidium paludigenum and sodium bicarbonate (SBC) treatments applied separately or in combination to control green mold of two cultivars of citrus fruit (Citrus reticulata Blanco cv. Ponkan and Citrus reticulata Blanco cv. Subcompressa) caused by Penicillium digitatum. In the assay of C. reticulata Blanco cv. Subcompress, R. paludigenum at 107 cells/ml alone significantly (P<0.05) reduced green mold incidence compared to the control, but was less effective than the 5% SBC alone. The combination of 107 cells/ml R. paludigenum and 5% SBC was as effective as fungicide (name of the fungicide), and significantly (P<0.05) reduced the decay incidence by 22.5%,40.1%, and 66.5%, compared to 107 cells/ml R. paludigenum, 5% SBC and water control, respectively. In the assay of C. reticulata Blanco cv. Ponkan, the treatment with R. paludigenum at 108 cells/ml and 2% SBC were equally effective and significantly (P<0.05) reduced the decay incidence, moreover the combination of the two treatments reduced the decay incidence to 0, which was as effective as fungicide (name of the fungicide). In the population dynamics studies, the survival ability of R. paludigenum in the citrus wounds was enhanced by SBC. The work demonstrated that the proper combination of R. paludigenum and sodium bicarbonate could be an alternative to synthetic fungicides for the control of postharvest green mold of citrus.
	TO cite this article:Zhu Ruiyu,Yu Ting,Zheng Xiaodong. Postharvest control of citrus green mold by the integration of sodium bicarbonate and Rhodosporidium paludigenum[OL].[14 November 2011] http://en.paper.edu.cn/en_releasepaper/content/4449592


	8. Purification and Characterization of a novel β-galactosidase from the newly isolated strain Erwinia rhapontici E602
	JIANG Yike,XIA Yu
	Food Science and Technology 24 June 2011
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	Abstract:A β-galactosidase producing bacterial strain E602 was isolated from frozen soil and identified to be Erwinia rhapontici. This strain was cultured at 26℃ with the induction of lactose for production of the β-galactosidase. This enzyme was then purified with ammonium sulfate fractionation, ion exchange and gel filtration chromatography for further studies of the enzymatic characteristics. The purified enzyme had a molecular weight of about 100 kDa. The optimum reaction temperature and pH of this enzyme was 40℃ and pH 7.0 respectively, indicating that this enzyme was a mesophilic neutral β-galactosidase. However, the enzyme retained about 15% activity at 10℃, which also suggested cold-adaptive property of this enzyme. Kinetics of this enzyme was studied and the Km and Vmax of this enzyme was 0.21 mmol/L and 263.16 μmol/mg min-1. Metal ions such as Li+, K+, Mg2+ and Zn2+ can activate the enzymatic activity obviously, while Cu2+ and Fe2+ inhibited the activity. The lactose hydrolysis in milk had been also investigated and results suggested that this enzyme may be suitable for milk processing.
	TO cite this article:JIANG Yike,XIA Yu. Purification and Characterization of a novel β-galactosidase from the newly isolated strain Erwinia rhapontici E602[OL].[24 June 2011] http://en.paper.edu.cn/en_releasepaper/content/4433503


	9. Preparation and Performance of Wheat Gluten Composite Membrane by Intermingling Nano-Silver
	Li Ying,Liu Runcong,Song Xianliang,Li Guoqing
	Food Science and Technology 24 August 2010
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	Abstract:Nano-particles have small size, surface and interface, quantum size, macro-tunnel effect and other special characteristics.To obtain wheat gluten membranes with a good strength, nano-silver was added in the preparation of the membrane to toughen the structure of nano-composite membrane. Performance of the composite membrane was studied by adding a series of different amount of nano-silver. The results indicated that the intermingling of nano-silver had an influence on the performance of the composite membrane in varying degrees. The tensile strength and tensile stretch of the composite membrane had an upward trend with an increasing dosage of nano-silver. However, the moisture permeability, oxygen permeability and break-resistant value of the composite membrane decreased first and increased later. When the density of nano-silver was 0.8×10-6, performance of the composite membrane was the best. In addition, the result of infrared spectroscopy showed that there were some interactions between nano-silver particles and wheat protein molecules.
	TO cite this article:Li Ying,Liu Runcong,Song Xianliang, et al. Preparation and Performance of Wheat Gluten Composite Membrane by Intermingling Nano-Silver[OL].[24 August 2010] http://en.paper.edu.cn/en_releasepaper/content/4382901


	10. Heat and Mass Transfer Mathematic Model of Cabbage Vacuum Cooling and Experimental Research
	Kan Ankang,Han Houde,Geng Jinliang
	Food Science and Technology 09 April 2010
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	Abstract:The cabbage was taken as the study object in this paper. The heat and mass transfer mathematical models for the cylindrical shape vegetable were built and the experimental method was introduced to prove them. The comparison and analysis, under the certain vacuum degree of the vacuum chamber, was done between the simulation and the experiment. And the differences of the vacuum degree in the chamber, the temperature change of the cabbage, the relative humidity in the chamber and the cabbage mass loss, in the experiment and the simulation, were all recorded. The reason of the parameters variance and existent deference was also analyzed in this paper. The mathematical models and the result was useful for the study on the cylindrical vegetable vacuum pre-cooling technology.
	TO cite this article:Kan Ankang,Han Houde,Geng Jinliang. Heat and Mass Transfer Mathematic Model of Cabbage Vacuum Cooling and Experimental Research[J].

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