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	1. An improved predictive assay for radiotherapy to human hepatoma measured by prematurely chromosome condensation technique
	Yang Jianshe
	Biology 28 December 2005
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:To investigate the radiation response of hepatoma, SMMC-7721 cells were irradiated with 60Co γ-rays. Initial chromatid breaks were measured by counting the number of chromatid breaks and isochromatid breaks. A dose-dependent increase in radiation-induced chromatid/isochromatid breaks was observed in G1 and G2 phase respectively. A good relationship was found between cell survival and chromatin breaks. This study implied that low LET radiation-induced chromatid/isochromatid breaks can be possibly used as a good predictor of radiosensitivity of SMMC-7721 hepatoma cells.
	TO cite this article:Yang Jianshe. An improved predictive assay for radiotherapy to human hepatoma measured by prematurely chromosome condensation technique[OL].[28 December 2005] http://en.paper.edu.cn/en_releasepaper/content/4728


	2. Transgenic rice plants expressing rice dwarf virus outer coat protein gene (S8) are moderately resistant to virus infection
	Zheng Honghong,Wei Chunhong,Chen Shengxiang,Chen Zhangliang,Y. Li
	Biology 19 December 2005
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:We have previously obtained transgenic rice plants (Oryza sativa L.) containing S8 gene which encodes the outer coat protein of the rice dwarf virus (RDV) by particle bombardment. In this study, it was confirmed that the S8 gene has been stably inherited and expressed in the selfed T1 and T2 progenies of the primary trangenic rice plant line T0-2. Virus resistance assays revealed that both T1 and T2 transgenic plants exhibited resistance to RDV infection, indicating stable transmission of the trait of resistance. This study is the first report about the Pathogen-derived resistance in rice dwarf virus, a member of phytoreovirus.
	TO cite this article:Zheng Honghong,Wei Chunhong,Chen Shengxiang, et al. Transgenic rice plants expressing rice dwarf virus outer coat protein gene (S8) are moderately resistant to virus infection[OL].[19 December 2005] http://en.paper.edu.cn/en_releasepaper/content/4449


	3. Identification of thidiazuron-induced ESTs expressed differentially during callus differentiation of alfalfa (Medicago sativa L. cv. Jinnan)
	Zhang Chun-Rong ,Huang Xue-Lin ,Wu Jian-Yong ,Feng Bi-Hong,Chen Yunfeng
	Biology 05 December 2005
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	Abstract:Embryogenic callus induced from petioles of alfalfa (Medicago sativa L. cv. Jinnan) and maintained on kinetin (KN)-containing B5h medium becomes non-embryogenic with differentiation of tracheary elements when kinetin in the medium is replaced by thidiazuron (TDZ). To investigate the molecular mechanisms underlying the TDZ-induced morphological competence transition, we cloned the differentially expressed sequence tags (ESTs) from TDZ-treated callus of alfalfa using suppression subtractive hybridization (SSH). The expression patterns of genes induced by TDZ were found to change throughout the process of TDZ treatment. A homeodomain-leucine zipper protein gene, MSHB1, which was induced by TDZ, may be a transcription factor in the TDZ-regulation of alfalfa callus differentiation. The induction of trehalose-6-phosphate phosphatase (TPP), which may decrease the level of trehalose-6-phosphate, and the induction of 1-aminocyclopropane-1-carboxylate synthase (ACS), which increases the level of ethylene, by TDZ may be responsible for the termination of somatic embryogenesis.
	TO cite this article:Zhang Chun-Rong ,Huang Xue-Lin ,Wu Jian-Yong , et al. Identification of thidiazuron-induced ESTs expressed differentially during callus differentiation of alfalfa (Medicago sativa L. cv. Jinnan)[OL].[ 5 December 2005] http://en.paper.edu.cn/en_releasepaper/content/4069

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