Authentication email has already been sent, please check your email box: and activate it as soon as possible.
You can login to My Profile and manage your email alerts.
If you haven’t received the email, please:
|
|
There are 113 papers published in subject: since this site started. |
Select Subject |
Select/Unselect all | For Selected Papers |
Saved Papers
Please enter a name for this paper to be shown in your personalized Saved Papers list
|
1. Study on the renaturation process of epidermal growth factor-like protein and its therapeutic effect on neuroinflammation | |||
Yang Jingru,He Huahong,He Huafeng,Wang Huaqian | |||
Biology 25 March 2023 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:Epidermal growth factor (EGF) plays an important role in improving the inflammatory response. In this study, cholera toxin A2 subunit, cell-penetrating peptide trans-transcription activator (TAT) and EGF were fused and recombinant protein EGF-CTA2-TAT were expressed by Escherichia coli. Two methods of renaturation, affinity chromatography and dialysis, were selected to renature the protein. The renaturation efficiency of these two methods were compared. Activities of recombinant protein on promoting BALB/c 3T3 cells proliferation in vitro were also studied. After that, mice model of neuroinflammation was established by using the Lipopolysaccharide (LPS) intraperitoneal injection. The purified and renatured recombinant EGF-like protein was administered intranasally for neuroinflammation treatment. The Morris water maze (MWM) test was used to evaluate the therapeutic effects. Our results showed that recombinant EGF-like protein had a therapeutic effect on LPS-induced neuroinflammation in mice, and could remarkably improve the learning and memory ability of mice. The recombinant EGF-like protein renatured and purified by affinity chromatography in one step shows the advantages of simple process, high yield, excellent activity and low cost, providing a reference for mass production of such active proteins. | |||
TO cite this article:Yang Jingru,He Huahong,He Huafeng, et al. Study on the renaturation process of epidermal growth factor-like protein and its therapeutic effect on neuroinflammation[OL].[25 March 2023] http://en.paper.edu.cn/en_releasepaper/content/4759631 |
2. Screening of strains from reconstituted tobacco sheet making high-yield enzymes and aroma | |||
Yang Chunxue,Deng Zhang,Liao xiang ru,Guan Zhengbing | |||
Biology 01 March 2023 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:Reconstituted tobacco sheet (RTS) is critical to reduce the harm of cigarettes and considered as a comprehensive utilization approach for tobacco wastes in the tobacco industries. The aim of this study was to screen out bacteria from RTS materials capable of producing a high total amount of enzymes and aroma. A total of 21 strains with unique morphologies were obtained. Based on the results of enzymes and aroma producing capacities of the strains, one strain was screened. This strain, which was named B-NS-7, was capable of producing a total amount of 696.3 U/mL protease activity, 238.4 U/mL amylase activity, 64.3 U/mL pectinase activity and 100.0 μg/mL aroma. Combined with the results of the 16S rDNA sequencing, physiological and biochemical characteristics B-NS-7 was identified as Bacillus subtilis. Through a series of single factor experiences, the results showed that under the conditions of inoculation concentration of 1%, fermentation temperature of 37 C and time of 24 h, the highest aroma content of concentrated solutions were 116.3 μg/mL. Neophytadiene was responsible for the majority of the fragrance enhancement, and B-NS-7 was identified as an aldehydic and alkene-flavoured strain. The sensory quality of tobacco concentration solutions was superior under optimal conditions. This strain has been deposited at the China General Microbiological Culture Collection Center (CGMCC No. 23716). By way of this study, B. subtilis B-NS-7 was intended to be used in the manufacturing of RTS through microbial fermentation to improve the quality and providing suggestions for the microbial development of the RTS industry. | |||
TO cite this article:Yang Chunxue,Deng Zhang,Liao xiang ru, et al. Screening of strains from reconstituted tobacco sheet making high-yield enzymes and aroma[OL].[ 1 March 2023] http://en.paper.edu.cn/en_releasepaper/content/4759360 |
3. Personalized prognostic signature for lung cancer based on 15 transcription-related gene pairs | |||
Liao Zili,Ren Zhihao,Zhang Boxiang,Zhu Ruiyu | |||
Biology 15 February 2023 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:Lung cancer is the most aggressive malignancy and the leading cause of cancer deaths worldwide. Currently, reliable biomarkers are lacking in the diagnosis, prognosis and treatment of lung cancer. Given the significant role of transcription factors in tumorigenesis and progression, we aimed to establish a signature based on transcription-related gene pairs for the first time to predict the prognosis of lung cancer patients. The gene expression data and clinical information of 1568 lung cancer patients were obtained from The Cancer Genome Atlas data portal (TCGA) and Gene Expression Omnibus (GEO) as a training cohort and validation cohort, respectively. Through univariate Cox analysis and Least Absolute Shrinkage and Selection Operator (LASSO) analysis, we screened 15 transcription-related gene pairs to construct the transcription-related prognostic signature. Based on this signature, the samples were classified into high-risk group and low-risk group. Kaplan-Meier analysis and independent prognostic analysis showed that transcription-related prognostic signature predicted overall survival in lung cancer patients (p < 0.001). Compared with multiple clinical and pathological factors, the results of multivariate Cox regression analysis indicated that the signature was an independent prognostic factor in patients with lung cancer. Further analysis revealed the cellular pathways associated with this signature and the relationship between this signature and immune cell content. In conclusion, we established for the first time the signature of transcription-related genes on prognosis as an indicator to assess the overall survival in lung cancer patients. Our study provides new ideas for developing cancer prognostic signature and discovering new drug targets. | |||
TO cite this article:Liao Zili,Ren Zhihao,Zhang Boxiang, et al. Personalized prognostic signature for lung cancer based on 15 transcription-related gene pairs[OL].[15 February 2023] http://en.paper.edu.cn/en_releasepaper/content/4759077 |
4. Recombinant expression, fermentation and characterization of a type Ⅲ human like collagen | |||
XIANG Zhixiang,GONG Jingsong,XU Zhenghong,SHI Jingsong | |||
Biology 12 May 2022 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:Collagen, the highest content protein in the body, as irreplaceable biological functions, and it is widespread concerned in food, beauty, and medicine with great market demand. The gene encoding hlCOLⅢ α1 chain fragment was integrated into Pichia pastoris genome after partial amino acids were substituted. Combined with promoter engineering and high-density fermentation technology, soluble secretory expression with the highest yield of 1.05 g L-1 was achieved using two-stage feeding method, and the purity could reach 96% after affinity purification. The determination of N/C-terminal protein sequence were consistent with the theoretical expectation, glycine shared 27.02% and proline 23.92% in amino acid analysis, which were in accordance with the characteristics of collagen. Mass spectrometry combined with Fourier transform infrared spectroscopy and circular dichroism revealed that the target product existed as homologous dimer and trimer in the broth , which laid a foundation for exploring the self-assembly of collagen. | |||
TO cite this article:XIANG Zhixiang,GONG Jingsong,XU Zhenghong, et al. Recombinant expression, fermentation and characterization of a type Ⅲ human like collagen[OL].[12 May 2022] http://en.paper.edu.cn/en_releasepaper/content/4757718 |
5. Comparative transcriptome analysis reveals the mechanism of enhanced microbial hyaluronan biosynthesis | |||
Yao Zhiyuan,Gong Jinsong,Su Chang,Li Heng,Xu Zhenghong,Shi Jinsong | |||
Biology 11 May 2022 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:This study reveals the genetic and biochemical changes underlying the enhanced hyaluronan (HA) biosynthesis in Streptococcus zooepidemicus. The selected mutants performed a steady improvement of HA yield after multiple rounds of atmospheric and room temperature plasma (ARTP) mutagenesis combining with novel bovine serum albumin / cetyltrimethylammonium bromide coupled high-throughput screening assay. Compared to the corresponding wild type strains, the HA yield of the obtained mutant (mutant-A17) greatly increased by 42.9% with concomitant accelerated growth. Transcriptome sequencing exhibits that distinct mutants have similar genetic changes. Regulation in direction of metabolic flow into the HA biosynthesis, by enhancing genes responsible for the biosynthesis of HA including hasB, glmU and glmM, weaking downstream gene (nagA and nagB) of UDP-GlcNAc and significantly down-regulating transcription of wall-synthesizing genes, which could cause accumulation of precursors. These associated regulatory genes may provide control point for the efficient synthesis of HA and engineering of the HA-producing cell factory. | |||
TO cite this article:Yao Zhiyuan,Gong Jinsong,Su Chang, et al. Comparative transcriptome analysis reveals the mechanism of enhanced microbial hyaluronan biosynthesis[OL].[11 May 2022] http://en.paper.edu.cn/en_releasepaper/content/4757714 |
6. Research progress of related methods for controlling targeted protein level | |||
MIn Gao,ChongSheng He | |||
Biology 10 August 2021 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:In this paper, we reviewed the progress of target protein degradation methods, with special attention to the use of plant hormones to stimulate the degradation. Protein is the key player of many biological processes, interfering the protein level precisely is both important for basic research and clinical application. There are many ways to control the protein level artificially in different levels such as CRISPR-Cas9, RNAi and so on. These methods show different limitations, for example, CRISPR-Cas9 is irreversible, RNAi can cause off-target effect. Targeted protein degradation is one of the ways to regulate the protein level in a post-translational level, it can affect the protein level very fast and reversibly. To achieve targeted protein degradation, different methods have been developed such as small-molecule induced degradation, photo-induced degradation, auxin induced degradation and so on. The core principle is to degrade the target protein in a controllable manner. | |||
TO cite this article:MIn Gao,ChongSheng He. Research progress of related methods for controlling targeted protein level[OL].[10 August 2021] http://en.paper.edu.cn/en_releasepaper/content/4755399 |
7. Characterization and isolation of estradiol degrading bacteria Acinetobacter sp. (7-1) Isolated from Changchun, China. | |||
YU Jian,ZHAO yanyang,HOU yue | |||
Biology 15 December 2020 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:17β- Estradiol, as a major environmental estrogen, is a serious threat to human health and ecological security. The MS medium containing 0.5 mM estradiol as the sole carbon source was used to isolate a new Gram-negative bacteria from the fishing pond. This screening strain can use as the sole carbon source and energy source such as estradiol and testosterone. After 16S rRNA analysis, the bacteria belong to Acinetobacter sp with 99.71% identity. The strain was Gram-negative bacilli, Spherical, Blunt round ends, Scattered or arranged in pairs, No bud, No flagella. Size 2.126μm×0.753μm; Conditions for optimum degradation of estradiol were 27℃, 150 rpm, and substrate concentration was 1 mM/L, pH7.5 and 1% inoculum. The highest degradation rate, the degradation rate can reach 85%. The strain could grow normally in 5 μg/mL auromycin, but not in 100 μg/mL ampicillin,50 μg/mL kanamycin,10 μg/mL streptomycin. The degradation rate of 17β- estradiol was 81.4%, that of testosterone was 64.6%, that of progesterone was 44.6%, and that of 17α- ethinyl estradiol was 62.3% by high performance liquid chromatography. | |||
TO cite this article:YU Jian,ZHAO yanyang,HOU yue. Characterization and isolation of estradiol degrading bacteria Acinetobacter sp. (7-1) Isolated from Changchun, China.[OL].[15 December 2020] http://en.paper.edu.cn/en_releasepaper/content/4753226 |
8. A review: Inhibitors and activators for paraoxonase 1 | |||
Li Yan,Tang Meiling,Chen Gang,Mu Xiaojing,Huang Haiming | |||
Biology 23 April 2020 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:Paraoxonase 1 (PON1) is a calcium-dependent hydrolase with many physiological functions. The decrease of PON1 activity is related to many diseases. The research about PON1 is a hot topic, and its activators and inhibitors are expected to help in our diets and in choose of drugs. The papers about PON1 activators and inhibitors were collected and classified. The PON1 activators and inhibitors are grouped into four categories: in-vivo activators, in-vitro activators, in-vivo inhibitors and in-vitro inhibitors. For each activator or inhibitor, the activating effect or inhibiting effect along with the methods used was reviewed. Up to now, we found from literatures that there were 16 samples demonstrating activating effects on PON1 in-vivo and 5 compounds showed in-vitro activation. Of the 16 in-vivo activators there were 4 plant extracts and 12 pure components. Seven in-vivo inhibitors and 71 in-vitro inhibitors have been reported in publications. Additionally, smoking, drinking and treatment with exogenous gonadotropins induced decrease of PON1 activity. The summary of the activators and inhibitors are beneficial to reveal the structure-activity relationship, and also it provides convenience to look up the activating or inhibiting effects of the compounds on PON1 at a glance. | |||
TO cite this article:Li Yan,Tang Meiling,Chen Gang, et al. A review: Inhibitors and activators for paraoxonase 1[OL].[23 April 2020] http://en.paper.edu.cn/en_releasepaper/content/4751765 |
9. TH upstream-inhibited DCI subnetwork for learning in human left hemisphere|Tonsil via nucleo to cytoplasm poly(A) RNA binding | |||
YANG Kaitong,WANG Lin,HUANG Juxiang | |||
Biology 24 December 2019 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:High tyrosine hydroxylase (TH) upstream-inhibited enoyl-CoA delta isomerase 1 (DCI) molecular subnetwork was constructed, including upstream NCK adaptor protein 2 (NCK2), retinoblastoma binding protein 6 (RBBP6), sulfotransferase family 1A member 2 (SULT1A2); downstream chromosome 10 open reading frame 10 (C10orf10), forkhead box N3 (FOXN3_2), poly (rC) binding protein 2 (PCBP2_2) reported relation with learning in human left hemisphere. The common biology process of TH upstream-inhibited DCI subnetwork was identified by DAVID, containing upstream NCK2, upstream RBBP6, upstream SULT1A2, downstream FOXN3_2, downstream PCBP2_2, first-core TH as protein binding; upstream SULT1A2, second-core DCI, first-core TH as small molecule metabolic process; upstream RBBP6, downstream PCBP2_2 as poly(A) RNA binding; downstream PCBP2_2, first-core TH as enzyme binding; The common cellular component of upstream NCK2, upstream RBBP6, downstream PCBP2_2, first-core TH as cytoplasm; upstream NCK2, upstream SULT1A2, downstream PCBP2_2, first-core TH as cytosol; downstream C10orf10, second-core DCI, first-core TH as mitochondrion; downstream FOXN3_2, downstream PCBP2_2, first-core TH as nucleus; upstream RBBP6, downstream PCBP2_2 as nucleoplasm; downstream PCBP2_2, second-core DCI as extracellular exosome; The common tissue distributions as Tonsil_3rd maybe exist the same pattern with human left hemisphere. We propose and mutual positively verify tyrosine hydroxylase (TH) upstream-inhibited enoyl-CoA delta isomerase 1 (DCI) subnetwork for learning in human left hemisphere|Tonsil via nucleo to cytoplasm poly(A) RNA binding. | |||
TO cite this article:YANG Kaitong,WANG Lin,HUANG Juxiang. TH upstream-inhibited DCI subnetwork for learning in human left hemisphere|Tonsil via nucleo to cytoplasm poly(A) RNA binding[OL].[24 December 2019] http://en.paper.edu.cn/en_releasepaper/content/4750327 |
10. Research progress of peptide durgs discovery | |||
Yin Xinong,Wu Lei | |||
Biology 18 November 2018 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:Peptides are a kind of active substances composed of amino acids, which play an important role in various progress of life activities, including immune defense, cell proliferation/differentiation, tumor pathological changes and so on. Since the first synthetic bioactive peptide was born in 1953, more than 80 polypeptide drugs have been listed in the global market, including some "blockbuster" drugs with sales of more than $1 billion. A large number of peptide drugs have entered the clinical trial or declaring stage. There are many unique advantages of peptide drugs: remarkable effectivity and specificity, less toxicity, low probability to accumulate in the body tissues and a handful of interaction with other drugs. Thus, more and more peptide drugs are widely used in the prevention, diagnosis and treatment of diabetes, tumors, cardiovascular diseases and hepatitis. This article reviewed the current research progress of peptide drugs, and prospects the great development of peptide drugs in the future. | |||
TO cite this article:Yin Xinong,Wu Lei. Research progress of peptide durgs discovery[OL].[18 November 2018] http://en.paper.edu.cn/en_releasepaper/content/4746489 |
Select/Unselect all | For Selected Papers |
Saved Papers
Please enter a name for this paper to be shown in your personalized Saved Papers list
|
|
About Sciencepaper Online | Privacy Policy | Terms & Conditions | Contact Us
© 2003-2012 Sciencepaper Online. unless otherwise stated