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1. Preliminary study on the function of 6mA demethylase in Arabidopsis thaliana | |||
Min Gao,Chongsheng He | |||
Biology 07 April 2022 | |||
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Abstract:N6-methyladenine (6mA) is the most conservative and common epigenetic marker in prokaryotes. The function and distribution of 6mA in eukaryotes, especially in plants, is still a mystery. It has been reported that 6mA does exist in plants and plays a vital role. However, how 6mA affects plant development, high temperature resistance and stress response is still unknown. 6mA has been proved to be a dynamic modification in eukaryotes, mediated by methyltransferase and demethylase. Arabidopsis thaliana is the model organism for plant biology and system biology research. In this study, we used Arabidopsis thaliana as material to identify 6mA demethylase in plants. Through bioinformatics analysis and in vitro enzyme activity experiment, 6mA demethylase in Arabidopsis thaliana has been found, which paved the road for the study of function and mechanism of demethylase in plants. | |||
TO cite this article:Min Gao,Chongsheng He. Preliminary study on the function of 6mA demethylase in Arabidopsis thaliana[OL].[ 7 April 2022] http://en.paper.edu.cn/en_releasepaper/content/4757339 |
2. TH upstream-inhibited CYFIP2 subnetwork for learning via cytoplasm to nucleus small GTPase mediated signal transduction | |||
ZHANG Weijian,HUANG Juxiang,WANG Lin | |||
Biology 23 December 2019 | |||
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Abstract:High tyrosine hydroxylase (TH) upstream-inhibited cytoplasmic FMR1 interacting protein 2 (CYFIP2) molecular subnetwork was constructed, including feedback SMAD family member 1 (SMAD1_2); downstream Rho GTPase activating protein 12 (ARHGAP12), forkhead box N3 (FOXN3_2) reported relation with learning in human left hemisphere. The common biology process of TH upstream-inhibited CYFIP2 subnetwork was identified by DAVID, containing feedback SMAD1_2, downstream FOXN3_2, second-core CYFIP2, first-core TH as protein binding; feedback SMAD1_2, downstream FOXN3_2 as transcription factor activity sequence specific DNA binding; downstream ARHGAP12, second-core CYFIP2 as small GTPase mediated signal transduction; The common cellular component of feedback SMAD1_2, downstream FOXN3_2, second-core CYFIP2, first-core TH as nucleus; feedback SMAD1_2, downstream ARHGAP12, second-core CYFIP2, first-core TH as cytosol; feedback SMAD1_2, second-core CYFIP2, first-core TH as cytoplasm; second-core CYFIP2, first-core TH as neuron projection; The common tissue distributions as ADIPOCYTE_3rd, CD8+T cells_3rd, PLACENTA_3rd, salivarygland_3rd maybe exist the same pattern with human left hemisphere. We propose and mutual positively verify tyrosine hydroxylase (TH) upstream-inhibited cytoplasmic FMR1 interacting protein 2 (CYFIP2) subnetwork for learning in human left hemisphere|ADIPOCYTE|CD8+T cells|PLACENTA|salivarygland via cytoplasm to nucleus small GTPase mediated signal transduction. | |||
TO cite this article:ZHANG Weijian,HUANG Juxiang,WANG Lin. TH upstream-inhibited CYFIP2 subnetwork for learning via cytoplasm to nucleus small GTPase mediated signal transduction[OL].[23 December 2019] http://en.paper.edu.cn/en_releasepaper/content/4750249 |
3. Vertical and Horizontal cDNA Subtractions Reveal Tissue- Specific Unigenes for Potato Tuberization | |||
Yao Xinling,Hong Zhiping,Wu Qing,Fan Huihua | |||
Biology 26 February 2010 | |||
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Abstract:Potato tuberization is a unique biologic process in Solanaceous plants. However, a few are known yet on which genes are involving in metabolism of the process. To dig genes related to tuberization in full transcriptome wide, with leafs and stems/stolons of the early- and late-mature genotypes, 4 vertical and 7 horizontal cDNA subtraction hybridizations were done and led to 11 cDNA libraries constructed. Libraries screening, ESTs sequencing, Reverse Northern Blotting and database search revealed that 217 unigenes from nucleus, choroplasm and mitochondrial genome expressed in leafs and stems/stolons related to tuberization. Data analysis indicated that 1) glucose metabolism companying by a strong redox activation in leafs, a week redox and catalysis level in stems leads to a late formation of stolon. 2) A strong redox activation resulting from lipid and protein metabolisms in stems and a low level of catalysis in leaf is necessary for a early stolon formation.3) it is dehydrin, an unique recognized metabolite, occurring in stems after, rather than before tuberzation. As a conclusion, changes of redox reactions and metabolism lead to either no or early/late tuberization. All above is identical with experiment conclusions reported and deepen our understanding of stolon and tuber formation. | |||
TO cite this article:Yao Xinling,Hong Zhiping,Wu Qing, et al. Vertical and Horizontal cDNA Subtractions Reveal Tissue- Specific Unigenes for Potato Tuberization[OL].[26 February 2010] http://en.paper.edu.cn/en_releasepaper/content/40261 |
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