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There are 149 papers published in subject: since this site started. |
Results per page: | 149 Total, 15 Pages | << First < Previous 12 13 14 15 |
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1. Extra divisions and nuclei fusions in microspores from Brassica allohexaploid (AABBCC) × Orychophragmus violaceus hybrids | |||
Ge Xianhong | |||
Agronomy 24 October 2006 | |||
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Abstract:Abnormal meiosis and microspore development and related defective mutants have often been reported in plants and wide hybrids. Here extra divisions and nuclei fusions were observed to occur in microspore nuclei of partial hybrids between synthetic Brassica hexaploid (2n=54, AABBCC) and another crucifer Orychophragmus violaceus (2n=24). Abnormal spindle were formed and chromosomes were separated into several nuclei of variable sizes after bi-, or multi-polar divisions in the four cells of tetrads. As a consequence, more than eight mini-microspores of different sizes were produced by one tetrad. Genomic in situ hybridization results indicated that no chromosome replication occurred during such divisions. In some tetrads, the four nuclei were fused to form one large cell with increased chromosome number. The extra divisions or fusions appeared only in some flower buds of one plant, some anthers in the same buds, or even in individual cells of tetrads. The possible mechanisms behind these cytological phenomena were discussed. | |||
TO cite this article:Ge Xianhong . Extra divisions and nuclei fusions in microspores from Brassica allohexaploid (AABBCC) × Orychophragmus violaceus hybrids[OL].[24 October 2006] http://en.paper.edu.cn/en_releasepaper/content/8933 |
2. Genome doubling and chromosome elimination with fragment recombination leading to the formation of Brassica rapa-type plants with genomic alterations in crosses with Orychophragmus violaceus | |||
Liu Min | |||
Agronomy 24 October 2006 | |||
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Abstract:In plant distant crosses, non-classical hybrids with unexpected chromosome complements, chromosome elimination and genetic introgression have been documented. Herein hybrid plants were obtained with embryo rescue from intergeneric crosses between Brassica rapa, B. rapa var. chinensis and another crucifer, Orychophragmus violaceus, and mainly displayed a female B. rapa phenotype but also certain O. violaceus or novel characteristics. Variable numbers of chromosomes appeared in somatic cells in the roots of plantlets on medium and ovaries and pollen mother cells (PMCs) and higher cell numbers were recorded in the roots. GISH observations revealed the majority of ovary cells and PMCs to twenty chromosomes of B. rapa with or without individual O. violaceus chromosomes or fragments added or introgressed. AFLP analysis revealed that fragments deleted for B. rapa was much more frequent than novel and O. violaceus fragments and about 50% of them were the same in more than half of F1 plants, and the number of O. violaceus fragments positively correlated with its traits expressed in hybrids. The mechanisms behind the results were the genome doubling and successive elimination of the O. violaceus chromosomes accompanied by the fragment recombination and introgression, producing B. rapa-type plants with modified genetic constitutions and phenotypes. | |||
TO cite this article:Liu Min . Genome doubling and chromosome elimination with fragment recombination leading to the formation of Brassica rapa-type plants with genomic alterations in crosses with Orychophragmus violaceus[OL].[24 October 2006] http://en.paper.edu.cn/en_releasepaper/content/8914 |
3. STS Markers for Powdery Mildew Resistance Gene Pm6 | |||
Ji Jianhui,Wang Haiyan,Cao Aizhong,Wang Suling,Zhuang Lifang,Chen Peidu,Liu Dajun,Wang Xiue | |||
Agronomy 18 October 2006 | |||
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Abstract:The powdery mildew resistance gene Pm6, which was from tetraploid wheat Triticum timopheevii L., is an effective gene in most powdery mildew epidemic areas of China. RFLP probe BCD135 has been proved linked with Pm6. In order to develop molecular markers for Pm6, in the present research, four STS primers (NAU/STSBCD135-1, NAU/STSBCD135-2, STS003 and STS004) were designed based on the sequence data of BCD135. These primers were used for PCR amplification using the genomic DNA of resistant near-isogenic lines (with Pm6) and their recurrent parent “Prins” as samples. No polymorphic product was observed when using primers STS003 and STS004. However, primers NAU/STSBCD135-1 and NAU/STSBCD135-2 could amplify two and one polymorphic bands between the near-isogenic-lines and “Prins”, respectively. The two primers were then used to amplify the F2 population from a cross of (IGV1-465 × Prins). The amplification and the powdery mildew resistance identification data were analyzed using the software Mapmaker3.0. The results indicated that both NAU/STSBCD135-1 and NAU/STSBCD135-2 were closely linked to Pm6 with a genetic distance of 1.0 cM. | |||
TO cite this article:Ji Jianhui,Wang Haiyan,Cao Aizhong, et al. STS Markers for Powdery Mildew Resistance Gene Pm6[OL].[18 October 2006] http://en.paper.edu.cn/en_releasepaper/content/8809 |
4. Isolation and Preliminary Identification of Aluminium-resistant Microorganisms from the Rhizophric Soil of Tea Plant | |||
Wu Ying,Liang Yuerong,Lu Jianliang,Liu Zusheng | |||
Agronomy 03 March 2006 | |||
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Abstract:Microorganism isolated from rhizospheric soil of tea plant was inoculated in culture medium containing aluminium 5-20 mmol/L and an aluminium-resistant fungus ALF-1 was isolated. The ALF-1 fungus possesses aluminium-resistant ability. It could grow normally in the medium containing 20 mmol/L of aluminium and decreased the aluminium content in medium and faded the haematocylin colour of the medium. The fungus was identified as a species of Neurospora sp. | |||
TO cite this article:Wu Ying,Liang Yuerong,Lu Jianliang, et al. Isolation and Preliminary Identification of Aluminium-resistant Microorganisms from the Rhizophric Soil of Tea Plant[OL].[ 3 March 2006] http://en.paper.edu.cn/en_releasepaper/content/5494 |
5. Factors affecting plant regeneration from cotyledon explants of cultivated pepper (Capsicum annuum L.) species | |||
Zhang Yuyang,Tang Yinzhu,Ye Zhibiao,Lu Yongen,Ouyang Bo,Zhang Chanjuan,Li Hanxia | |||
Agronomy 21 February 2006 | |||
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Abstract:A detailed study was carried out to investigate key factors affecting bud induction and shoot elongation of pepper regeneration, and develop a reliable and highly efficient in vitro regeneration system. Ability for in vitro shoot regeneration and elongation is genotype dependent. “Chufeng” displayed the highest regeneration response: 100% frequency of adventitious bud induction with all hormone combinations tested. The fully expanded cotyledons of 8–12-day-old seedlings gave the highest regeneration efficiency. Adventitious buds subjected to 21-day-induction produced higher elongation efficiency than that of 14-day-induction. The hormone combination of Zeatin + gibberellic acid generated the most efficient bud elongation. The media for in vitro regeneration from cotyledon explants of pepper were optimized: the buds induction medium: Murashige and Skoog medium+ 6-benzyl-aminopurine 6.0 mg l–1 + indole-3-acetic acid 1.0 mg l–1; the shoots elongation medium: Murashige and Skoog salts + B5 vitamins + Zeatin 2.0 mg l–1 + gibberellic acid 1.5 mg l–1; the rooting medium: half-strength MS + 0.5 mg l–1 indole-3-butyric acid. Influence of other factors such as Ag+ and seedling nutrient juice on regeneration was also studied. In general, high frequency of regeneration was obtained from all varieties tested. Plantlets were transplanted to soil and acclimatized in the greenhouse showing normal development and grown to maturity bearing normal fruits with seeds. An overall optimized scheme for pepper regeneration in vitro from cotyledons was presented, through which 100% bud induction and 50%–100% elongation efficiency was obtained. | |||
TO cite this article:Zhang Yuyang,Tang Yinzhu,Ye Zhibiao, et al. Factors affecting plant regeneration from cotyledon explants of cultivated pepper (Capsicum annuum L.) species[OL].[21 February 2006] http://en.paper.edu.cn/en_releasepaper/content/5284 |
6. Wolbachia infection and its influence on the reproduction of stored-product psocid, Liposcelis tricolor (Psocoptera: Liposcelididae) | |||
Dong Peng,Wang Jinjun,Zhao Zhimo | |||
Agronomy 30 January 2006 | |||
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Abstract:Wolbachia are maternally inherited intracellular bacteria that infect a wide range of arthropods and nematodes and are associated with various reproductive abnormalities in their hosts. The infection of Wolbachia in the species of psocid, Liposcelis tricolor, was investigated through Long PCR amplification of wsp gene. The results showed that the population of L. tricolor was positive for Wolbachia. The phylogenetic relationship of Wolbachia presented in L. tricolor and in other insect hosts was also analyzed. The result showed that Wolbachia strain presented in host of L. tricolor belongs to the B-group. Wolbachia infection in host of L. tricolor could be removed through antibiotic treatment. The results of crosses, including ♀w+×♂w+,♀w-×♂w+ ,♀w+×♂w- and ♀w-×♂w-, suggested that the removal of Wolbachia has resulted in lower eggs production of L. Tricolor. The mean embryonic mortalities of offspring produced by L. tricolor without Wolbachia were significantly higher than that of control. | |||
TO cite this article:Dong Peng,Wang Jinjun,Zhao Zhimo. Wolbachia infection and its influence on the reproduction of stored-product psocid, Liposcelis tricolor (Psocoptera: Liposcelididae)[OL].[30 January 2006] http://en.paper.edu.cn/en_releasepaper/content/5142 |
7. Toxicology and Biochemical Characteristics of GSTs in Liposcelis bostrychophila Badonnel (Psocoptera: Liposcelididae) | |||
Dou Wei,Wang Jinjun,Zhao Zhimo | |||
Agronomy 30 January 2006 | |||
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Abstract:The toxicological and biochemical characteristics of glutathione S-transferases (GSTs) in the resistant and susceptible strains of Liposcelis bostrychophila were investigated. Compared to their susceptible counterparts, the activities per insect and specific activities of GSTs in the DDVP- and PH3- resistant strains (DDVP-R and PH3-R) were significantly higher. The apparent Michaelis-Menten constant ( Km ) for CDNB were obviously lower in DDVP-R and PH3-R (i.e. lower Km values, 1.5625 mM for DDVP-R and 0.6230 mM for PH3-R) as compared with their susceptible counterpart (Km=3.5520), indicating a higher affinity to the substrate CDNB in resistant strains. In contrast, the catalytic activity of GSTs towards CDNB in susceptible strain was significantly higher than those in resistant strains. It was noticeable that when GSH used as substrate, GSTs from resistant strains both indicated a significantly declined affinity. For the catalytic activity of GSTs towards GSH, only the Vmax value in DDVP-R increased significantly compared to that from susceptible strain, suggesting an over expression of GST in this resistant strain. The inhibition kinetics of insecticides to GSTs in vitro revealed that dichlorvos and paraoxon possessed excellent inhibition effects on GSTs. The susceptible strain showed higher sensitivity (I50=0.9004 mM) to dichlorvos than DDVP-R and PH3-R (higher I50s, 8.0955 mM for DDVP-R and 9.3346 mM for PH3-R). As for paraoxon, there was a similar situation. The resistant strains both suggested a higher I50 (1.8735 mM for DDVP-R, and 0.4291 for PH3-R) compared to the susceptible strain (0.2943 mM). These suggested that an elevated detoxification ability of GSTs developed in the resistant strains. | |||
TO cite this article:Dou Wei,Wang Jinjun,Zhao Zhimo. Toxicology and Biochemical Characteristics of GSTs in Liposcelis bostrychophila Badonnel (Psocoptera: Liposcelididae) [OL].[30 January 2006] http://en.paper.edu.cn/en_releasepaper/content/5139 |
8. Regulation of Root Cluster Formation and Citrate Exudation in White Lupin (Lupinus albus L.) Plants | |||
LI Chun-Jian,LIANG Rui-Xia | |||
Agronomy 05 December 2005 | |||
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Abstract:Split-root system was used to investigate whether the external or internal P concentration controls root cluster formation and citrate exudation in white lupin (Lupinus albus L.) grown under the controlled conditions. In spite of low P concentration in shoot and root of the -P plant, its dry weight was not reduced, compared with the +P plant. Supplying external P (0.25 mmol/L) to one root half resulted in increase in P concentration not only in the shoot, but also in the P deprived root half, indicating P cycling within the plants. P omitting from both split-root pots stimulated root cluster formation in both root halves. While P supply to one root half stimulated root cluster formation at the beginning of the treatment. However, neither P supply to just one root half continuously nor re-supply of P to one root half after 19 d of P starvation could inhibited root cluster formation in the P deprived side, although the P concentration in this root half and shoot increased markedly. The results indicated that root cluster formation in Lupinus albus was controlled by both shoot and root P concentration. The rates of citrate exudation by both root halves with P-deficiency were higher than that of one root half supplied with P only. In the treatment with one root half supplied with P, the rates of citrate exudation by either the P-supplied or deprived root halves were almost same regardless of P concentration in the roots. The results suggested that internal P concentration controlled root cluster formation and citrate exudation in white lupin, but they might be regulated by different mechanisms. | |||
TO cite this article:LI Chun-Jian,LIANG Rui-Xia. Regulation of Root Cluster Formation and Citrate Exudation in White Lupin (Lupinus albus L.) Plants[OL].[ 5 December 2005] http://en.paper.edu.cn/en_releasepaper/content/4064 |
9. Seeding Precision Test Based on Machine Vision | |||
LIN Jiachun,Liwei | |||
Agronomy 28 January 2005 | |||
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Abstract:eeding uniformity is a crucial index to seeding performance and quality of a grain seeder. A testing approach for seeding precision was developed in this paper, which is an integrated technology of machine vision, pattern recognition, and automatic control. The new method realizes a precise and reliable test of seeding precision, and an automatic control of tester operation. A machine vision based test-bed was developed for performance tests of grain seeders, such as a precision planter, a drill planter, a hill-drop planter, and a corresponding software package was compiled to capture the images of the deposited seeds, to segment the seeds from the background of an image, and to calculate the distances between seeds after precision seeding, the number of seeds per length after drill seeding, and the distance between hills and number of seeds per hill after hill-drop seeding. The date obtained above was then used to estimate the performance of a seeder by the statistical calculations. | |||
TO cite this article:LIN Jiachun,Liwei. Seeding Precision Test Based on Machine Vision[OL].[28 January 2005] http://en.paper.edu.cn/en_releasepaper/content/1511 |
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