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| There are 27 papers published in subject: > since this site started. | |||
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| 1. iTRAQ -based quantitative proteomics analysis of upland cotton (Gossypium hirsutum) stem terminal buds reveals phytohormone related pathways associated with dwarfism | |||
| TU Xiaoju,WANG Qiming,LI Juan,LIU Aiyu | |||
| Agronomy 26 July 2015 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:Identifying important regulative elements and pathways related to dwarf in cotton is a major challenge in cotton breeding. An iTRAQ-based proteomics of stem terminal buds from the upland cotton species Gossypium hirsutum dwarf line LA-1 and high near-isogenic line （NIL） LH-1 was performed. Moreover, the transcriptional expression of differentially expressed proteins (DEPs) belonged to phytohormone signal transduction, hormone biosynthesis and ubiquitin system, were analyzed using the quantitative real-time polymerase chain reaction (PCR). A total of 4, 849 proteins were identified from LA-1 and LH-1, 697 of which showed differential accumulations. Most of DEPs function as catalytic activity, binding and transporter activity, and involved in metabolism and protein processing related pathways. In particular, 7 DEPs, including 2 of gibberellins (GA) receptor, 3 of cytokinin (CK) receptor, CK oxidase and CK-N-glucosyltransferase were up-regulated in LA-1, and gibberellin 20-oxidase was down-regulated in LA-1. Our results suggest that DELLA-independent GA signaling pathway is primary reason caused the dwarf in LA-1, and indicate that CRE1-2, GID, CKX and GA20ox are potential indicators of dwarf cotton, and the profiling of DEPs may offer a valuable resource for molecular markers of dwarf cotton breeding. | |||
| TO cite this article:TU Xiaoju,WANG Qiming,LI Juan, et al. iTRAQ -based quantitative proteomics analysis of upland cotton (Gossypium hirsutum) stem terminal buds reveals phytohormone related pathways associated with dwarfism[OL].[26 July 2015] http://en.paper.edu.cn/en_releasepaper/content/4650289 | |||
| 2. Modeling of rapeseed at maturity stage using 3D unorganized point clouds and digital images | |||
| CHANG Tingting,WANG Xingyu,ZHAI Ruifang | |||
Agronomy 15 September 2014
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| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:The 3D morphological expressin of a plant is important because this is the basis for building a functinonal-structural plant model to study the relationships between the structure, its environment, and its internal biology. Creating 3D plant models is often a difficult and laborious task especially to rapeseed because of its complexity and it has not been accomplished in the traditional ways. To make it easier and more natural, the integration of digital images and 3D unorganized point clouds from a digitizer provides a promising approach for rapeseed model generation. In the present study, 3D unorganized point clouds and digital images were incorporated in the generation of complex models of rapeseeds at maturity stage. Unorganized point clouds and image sequences were taken from different viewpoints using a 3D digitizer. The 3D unorganized points and image sequences were used for the automated registration of all data sets from all the viewpoints, which is pair-wise registration. Later, all the pair-wise registration parameters were used as initial transformation parameters for multiple registrations. The next procedure generated a surface model by triangulated irregular network using all the point clouds. The capabilities of our system were demonstrated through real data sets. Meanwhile, mesurements on some parameters such as branch locations, angles, lengths were implemented and compared to the parameters obtained by manual measurements. Experimental results showed that the average normal distances between the two scans were less than 0.3 mm after simultaneous registration, which indicated that the proposed methodology is effective and efficient. | |||
| TO cite this article:CHANG Tingting,WANG Xingyu,ZHAI Ruifang. Modeling of rapeseed at maturity stage using 3D unorganized point clouds and digital images[OL].[15 September 2014] http://en.paper.edu.cn/en_releasepaper/content/4610117 | |||
| 3. Abscisic acid, ethylene and antioxidative systems in rice grains in relation with grain filling subjected to postanthesis soil-drying | |||
| ZHANG Hao,LIU Kai,WANG Zhiqin,LIU Lijun,YANG Jianchang | |||
| Agronomy 13 August 2014 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:This study tested the hypothesis that abscisic acid (ABA) and ethylene may mediate the effect of postanthesis soil-drying on grain filling of rice (Oryza sativa L.) through regulating antioxidative systems. Two rice cultivars were pot-grown, and three soil-drying treatments, well-watered (WW), moderately soil-drying (MD) and severely soil-drying (SD), were imposed from 9 days post anthesis until maturity. Levels of ABA and ethylene, activities of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), and contents of superoxide anion (O2-) and malondialdehyde (MDA) and their relationships with grain filling rate were investigated. The results showed that, when compared with the WW treatment, the MD treatment increased, whereas the SD treatment decreased, grain filling rate and activities of CAT, POD, and SOD in both superior and inferior spikelets. Both MD and SD treatments increased contents of ABA, superoxide anion and MDA in grains, with more increases under the SD than under the MD. The MD treatment decreased, whereas the SD treatment increased, ethylene evolution rate and 1-aminocycopropane-1-carboxylic acid (ACC) content. The ratio of ABA to ACC (ABA/ACC) was the highest under the MD and the lowest under the SD among the three soil-drying treatments. ABA content, activities of CAT, POD, SOD, and ABA/ACC very significantly and positively, while the levels of ethylene and ACC and contents of superoxide anion and MDA significantly and negatively, correlated with grain filling rate. Application of cobalt ion (inhibitor of ethylene synthesis) or ABA at early grain filling stage significantly decreased contents of superoxide anion and MDA, and increased activities of CAT, POD, SOD and grain filling rate. Spraying with ethephon (ethylene-releasing agent) or fluridone (inhibitor of ABA synthesis) had the opposite effect. The results suggest that an increase in ABA and decrease in ethylene in the grains under postanthesis moderate soil-drying could enhance the activities of antioxidative enzymes and reduce free radicals in grains, and therefore, accelerate the grain filling rate of rice. | |||
| TO cite this article:ZHANG Hao,LIU Kai,WANG Zhiqin, et al. Abscisic acid, ethylene and antioxidative systems in rice grains in relation with grain filling subjected to postanthesis soil-drying[J]. | |||
| 4. Fine mapping and candidate gene analysis of gl-1, a gene controlling trichome formation in rice | |||
| ZENG Ruizhen,LIU Ziqiang | |||
| Agronomy 24 December 2013 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:Leaf trichome plays an important role in protecting plants against insect herbivores, loss of water through transpiration and UV irradiation. In our present study, SSSL-W24, a single segment substitution line (SSSL) containing only one chromosome segment of the glabrous japonica cultivar Star bonnet 99 in the genetic background of the pubescent indica cultivar HJX74 showed glabrous leaves and hulls. Genetic analysis of the F2 generation, derived from a cross between SSSL-W24 and HJX74, showed that these traits are controlled by the recessive gene gl-1, which was mapped to the short arm of chromosome 5. Fine mapping and high-resolution linkage analysis using 1585 F3 plants and markers ?anking gl-1 were carried out, and the gene was localized to a 35.9 kb region that contains seven annotated genes according to the genome sequence of japonica Nipponbare. Positional cloning of this gene will assist selection of hybrid rice, facilitate the mechanization of agriculture and increase the warehousing capacity of rice. | |||
| TO cite this article:ZENG Ruizhen,LIU Ziqiang. Fine mapping and candidate gene analysis of gl-1, a gene controlling trichome formation in rice[OL].[24 December 2013] http://en.paper.edu.cn/en_releasepaper/content/4577925 | |||
| 5. Comparative analysis of mitochondrial genomes between the hau cytoplasmic male sterility (CMS) line and its iso-nuclear maintainer line in Brassica juncea to reveal the origin of the CMS-associated gene orf288 | |||
| Heng Shuangping,Wei Chao,Jing Bing,Wan Zhengjie,Wen Jing,Yi Bin,Ma Chaozhi,Tu Jinxing,Fu Tingdong,Shen Jinxiong | |||
| Agronomy 20 November 2013 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:Cytoplasmic male sterility (CMS) is not only important for exploiting heterosis in crop plants, but also as a model for investigating nuclear-cytoplasmic interaction. CMS may be caused by mutations, rearrangement, or recombination in the mitochondrial genome. With the use of the next-generation sequencing technology, we sequenced the mitochondrial genome of hau CMS line and its maintainer line in B. juneca. The result showed that B. juncea hau CMS mitochondrial genome was assembled into a single, circular-mapping molecule with a size of 247,903 bp with 45.08% GC content. The genome contains 35 proteins, 3 rRNAs, 25 tRNA genes and 29 ORFs of unknown function in addition to the CMS associated gene orf288. The sizes of the maintainer line and another normal type line "J163-4" are both 219,863 bp and the GC content is 45.23%. The maintainer line contains 36 proteins, 3 rRNAs, and 22 tRNA genes and 31 unidentified ORFs. After the comparative analysis the hau CMS line and its maintainer line, specific markers were developed based on their unique ORFs. These can be used as an effective markers to separate the hau CMS line from its maintainer line at the seedling stage. We also confirmed that substoichiometrically different mitotypes coexist in hau CMS lines and its maintainer lines in B. juncea. The number of repeats larger than 100bp in the hau CMS line (16 repeats) are almost twice those of the maintainer line (9 repeats). The 5' region of the chimeric gene orf288 is highly similar to nad5, and the 3' region is also similar to another predicted mitochondrial ORF-orf293 in B. juncea. Phylogenetic analysis of the CMS-associated gene orf288 and four other homologous sequences in Brassicaceae showed that orf288 bore strong similarity to orf263 in Brassica tournefortii, but they still differed from one another. The hau CMS mitochondrial genome was highly rearranged when compared with its iso-nuclear maintainer line mitochondrial genome as the other CMS lines in different crops. The gene orf288 was composed of part nad5, orf293 and an unknown origin in the 5' region as orf263 reported in Brassica tournefortii. It may help us identify the mechanism for natural CMS in B. juncea, and compared with the sequenced mitochondrial genomes in Brassicas, uncover the origin of the hau CMS mitotype and structural and evolutionary differences between different mitotypes. | |||
| TO cite this article:Heng Shuangping,Wei Chao,Jing Bing, et al. Comparative analysis of mitochondrial genomes between the hau cytoplasmic male sterility (CMS) line and its iso-nuclear maintainer line in Brassica juncea to reveal the origin of the CMS-associated gene orf288[J]. | |||
| 6. A Theoretical Model Research of Rice Water Stress Index based on Infrared Thermal Imaging | |||
| GAO Mingchao,ZHANG Wenzhong,YAO Chen,YIN Dawei,CHENG Xiaoyi,PEI Yao,YU Jilin,ZHAO Miaomiao | |||
| Agronomy 16 May 2013
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| Abstract:It detected the canopy temperature of rice via automated infrared imaging technology in the test under different irrigation condition, and used the CWSI theoretical model to diagnose whether the crop suffered water stress or not. It also analyzed the water stress index theoretical model of crop and other indexes on reflecting the water status of crop, including the relationship between the CWSI and leaf stomatal resistance, the CWSI and leaf net photosynthetic rate, and the CWSI and the soil moisture content. The results showed that the relations between the surface theoretical model and the above indexes were fine. It meant the CWSI well reflected the features of water stress of rice. | |||
| TO cite this article:GAO Mingchao,ZHANG Wenzhong,YAO Chen, et al. A Theoretical Model Research of Rice Water Stress Index based on Infrared Thermal Imaging[OL].[16 May 2013] http://en.paper.edu.cn/en_releasepaper/content/4543723 | |||
| 7. Based on Infrared Thermal Imaging Technology in Rice Water Stress Index Test | |||
| GAO Mingchao,ZHANG Wenzhong,HAN Yadong,YAO Chen,WANG Yitao,DING Guohua,ZHANG Zhenyu | |||
Agronomy 28 February 2013
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| Abstract:Observed by infrared thermal imaging technology, in the test rice canopy temperature were measured under different irrigation conditions, taking the Idso empirical model to determine the lower bound equation of the canopy-air temperature differences, and so to get the best period of observing the rice water deficit by the CWSI, in addition the relationship of the CWSI, soil water content and transpiration rate were also analyzed. The preliminary test analysis showed that the CWSI can reflect the rice water stress conditions well. | |||
| TO cite this article:GAO Mingchao,ZHANG Wenzhong,HAN Yadong, et al. Based on Infrared Thermal Imaging Technology in Rice Water Stress Index Test[OL].[28 February 2013] http://en.paper.edu.cn/en_releasepaper/content/4524293 | |||
| 8. The arrest of megagametophyte development results in the female sterility of a dominant genic male-sterile line in Brassica napus | |||
| Han Xue,Hong Dengfeng,Yang Guangsheng | |||
Agronomy 04 January 2013
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| Abstract:The phenomenon of female sterility on all male-sterile plants was observed in a genic male-sterile (GMS) Brassica napus line designated as FM195AB (A represents sterile plants and B refers to its wild type ones). For the purpose of clarifying the causal mechanism of this female sterility, we contrastively studied the morphological characteristics of reproductive structures and procedures involved in seed formation between FM195A and FM195B. First, Scanning electron microscopy (SEM) investigations on stigma papilla cells showed no apparent difference between the fertile and sterile plants. Second, alien pollen grains can normally germinate and pollen tubes can enter the stigma papilla cells of both FM195A and FM195B; however, pollen tubes in FM195A pistils appear morphologically abnormal in comparison with FM195B, even if they can normally elongate and reach the ovules. Furtther observations of the paraffin-sectioned pistils revealed that early magasporogenesis in FM195A is similar to that of FM195B; however, mono-nucleate and the subsequent developmental stages of the female gametophytes can hardly seen in the ovules of FM195A, suggesting. Approximately at the eight-nucleate embryo sac stage in FM195B, five major types of the abnormal embryo sacs in FM195A can be observed. Similarly, we found that microsporogenesis in FM195A is also stopped before the entrance of meiosis. Because both of the male and female sterility occur at the same plant, it can be assumped that the male sterility gene, Ms, may play an important role in prompting the transition from maintaince of the spore mother cells into meiosis. | |||
| TO cite this article:Han Xue,Hong Dengfeng,Yang Guangsheng. The arrest of megagametophyte development results in the female sterility of a dominant genic male-sterile line in Brassica napus[OL].[ 4 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4509274 | |||
| 9. Identification of major QTL for silique traits in Brassica napus | |||
| Qi Liping,Mao Long,Sun Chengming,Fu Tingdong,Shen Jinxing,Tu Jinxing,Ma Chaozhi,Wen Jing,Yi Bin | |||
| Agronomy 13 December 2012 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:Silique is the most important photosynthetic organ in the later period of rapeseed growth. Silique length (SL), number of seeds per silique (SN) and thousand seed weight (TSW) are known as direct or indirect factors influencing seed yield in Brassica napus. One hundred and eighty-one double haploid (DH) lines derived from two lines with distinct silique traits were employed to map quantitative trait loci (QTL) for SL, SN and TSW in B. napus. Field data from three environments were collected for correlation analysis and QTL mapping for SL, SN and TSW. The broad-sense heritability of SL, SN and TSW was 95.24%, 67.24% and 91.01%, respectively, suggesting that genetic variance played a leading role in total variations for SL and TSW. 37 consensus QTL and 31 unique QTL were identified by two-round meta-analysis. Among the 37 consensus QTL, two, two, four QTL detected on A9, A9 and A1 linkage group had effects on SL, TSW and SN. It could explain more than16.22-64.36% of trait variation. In addition, two, two, and three pairs of additive by additive (AA) interactions with small effects were detected for SL, SN and TSW, respectively. Our results will provide a basis for map-based cloning of QTL affecting silique traits and marker-assistant selection (MAS) in breeding of new rapeseed variety exhibiting higher seed yield. | |||
| TO cite this article:Qi Liping,Mao Long,Sun Chengming, et al. Identification of major QTL for silique traits in Brassica napus[OL].[13 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4500697 | |||
| 10. BnTst-1, A Tourist-like MITE transposon closely associated with genes and may play important roles in gene expression and species evolution in Brassica species | |||
| Hou Jinna,Long Yan | |||
| Agronomy 15 November 2012 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract:In this paper, a Tourist-like MITE BnTst-1 was identified in Brassica A and C genome. The BnTst-1 copy number and its distribution in different chromosomes in the genome of three Brassica species were investigated. The sequence variation of BnTst-1 and the TIR and TSD were compared among two Brassica species. The amplification profile diversity caused by BnTst-1 activity intra- and inter- species were confirmed experimentally. DNA methylation level of the BnTst-1 and its flanking sequences, together with EMSA experiments showed that this MITE may play regulation role in gene expression and genome evolution in Brassicaea. | |||
| TO cite this article:Hou Jinna,Long Yan. BnTst-1, A Tourist-like MITE transposon closely associated with genes and may play important roles in gene expression and species evolution in Brassica species[OL].[15 November 2012] http://en.paper.edu.cn/en_releasepaper/content/4495960 | |||
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