Abstract:Purposes: To investigate the distribution of human papillomavirus (HPV) genotypes in uterine cervical lesions in Yanbian of Northern China. Materials and Methods: Paraffin embedded blocks of 62 cases of squamous cell carcinomas (SCC), 28 cases of adenocarcinomas, and 143 cases of CIN (CIN-1: 45; CIN-2: 46; CIN-3: 52) were selected from Dept. of Pathology, Yanbian University Hospital and Yanbian Women’s Hospital in the period of 2000-2005. DNA was extracted by using High Pure PCR Template Preparation Kit from all above blocks. And HPV genotypes were detected by using oligonucleotide microarray (HPV DNA-chip). Results: All the 20 cases of normal cervical epithelia are negative for HPV by both TaKaRa PCR and DNA-chip methods. The positive rate of high-risk HPV is 28.9% in CIN-1, 41.3% in CIN-2, 44.2% in CIN-3, 85.5% in SCC, and 82.1% in adenocarcinoma by TaKaRa PCR method. Similarly, it is 26.7% in CIN-1, 41.3% in CIN-2, 40.4% in CIN-3, 82.3% in SCC, and 78.6% in adenocarcinoma by HPV DNA-chip. HPV 16 is the major type in all CIN and SCC cervical lesions. However, in cervical adenocarcinoma, HPV 18 is the most common type (59.1% in HPV positive cases), and HPV 16 is the second type, but still show a high percentage (31.8% in HPV positive cases). There is small number of multi-types HPV detected in CIN-3, SCC, and adenocarcinoma, but none in CIN-1/2. Meanwhile, there is no significant difference on the HPV screening between TaKaRa PCR and HPV DNA-chip methods (p<0.05). Conclusions: HPV 16 is the type most frequently involved in the development of SCC of the cervix, whereas both HPV 18 and 16 play a prominent role in the development of adenocarcinoma of the cervix in Yanbian of Northern China. Both TaKaRa PCR and HPV DNA-chip methods are sensitive for HPV detection, and can be used for the screening of HPV infection status and genotyping in uterine cervical lesions, and maybe helpful for the prediction of the development and progress of CIN-2/3.

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	1. The distribution of human papillomavirus genotypes in uterine cervical lesions in Yanbian of Northern China
	Zhao Yiwei ,Lin Hai ,Wu Qunying ,Han Songying ,Zhang Meihua,Lin Zhenhua
	Basic Medicine 09 October 2007
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	Abstract:Purposes: To investigate the distribution of human papillomavirus (HPV) genotypes in uterine cervical lesions in Yanbian of Northern China. Materials and Methods: Paraffin embedded blocks of 62 cases of squamous cell carcinomas (SCC), 28 cases of adenocarcinomas, and 143 cases of CIN (CIN-1: 45; CIN-2: 46; CIN-3: 52) were selected from Dept. of Pathology, Yanbian University Hospital and Yanbian Women’s Hospital in the period of 2000-2005. DNA was extracted by using High Pure PCR Template Preparation Kit from all above blocks. And HPV genotypes were detected by using oligonucleotide microarray (HPV DNA-chip). Results: All the 20 cases of normal cervical epithelia are negative for HPV by both TaKaRa PCR and DNA-chip methods. The positive rate of high-risk HPV is 28.9% in CIN-1, 41.3% in CIN-2, 44.2% in CIN-3, 85.5% in SCC, and 82.1% in adenocarcinoma by TaKaRa PCR method. Similarly, it is 26.7% in CIN-1, 41.3% in CIN-2, 40.4% in CIN-3, 82.3% in SCC, and 78.6% in adenocarcinoma by HPV DNA-chip. HPV 16 is the major type in all CIN and SCC cervical lesions. However, in cervical adenocarcinoma, HPV 18 is the most common type (59.1% in HPV positive cases), and HPV 16 is the second type, but still show a high percentage (31.8% in HPV positive cases). There is small number of multi-types HPV detected in CIN-3, SCC, and adenocarcinoma, but none in CIN-1/2. Meanwhile, there is no significant difference on the HPV screening between TaKaRa PCR and HPV DNA-chip methods (p<0.05). Conclusions: HPV 16 is the type most frequently involved in the development of SCC of the cervix, whereas both HPV 18 and 16 play a prominent role in the development of adenocarcinoma of the cervix in Yanbian of Northern China. Both TaKaRa PCR and HPV DNA-chip methods are sensitive for HPV detection, and can be used for the screening of HPV infection status and genotyping in uterine cervical lesions, and maybe helpful for the prediction of the development and progress of CIN-2/3.
	TO cite this article:Zhao Yiwei ,Lin Hai ,Wu Qunying , et al. The distribution of human papillomavirus genotypes in uterine cervical lesions in Yanbian of Northern China[OL].[ 9 October 2007] http://en.paper.edu.cn/en_releasepaper/content/15558


	2. STUDY ON THE DIFFERENTIATION OF MOUSE EMBRYONIC GERM CELLS INTO HEPATOCYTES
	FENG ZHI-HUI,LIU XIAO-PING,Guo Feifei
	Basic Medicine 02 April 2007
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	Abstract:Objective To investigate the condition which can induce embryonic germ cells (EGCs) to differentiate into hepatocytes in vitro, and lay bases for the study of mechanism of EGCs differentiation, so as to seek new source of seed cells for tissue engineering . Methods The EGCs deriving from gonadal ridges of the mouse embryos on the 11th day were cultured and proliferated in vitro. An aliquot EGCs suspension was cultured in 6-well dishes for 24 hours and then bFGF ,EGF, β-NGF, RA and hepatocyte abstraction were added into the dishes respectively for directional differentiation, and another aliquot EGCs was co-cultured with embryonic hepatic cells for differentiation. Morphological differentiation and intake of indiocyanine green (ICG) by EGCs were investigated. The committed differentiation of EGCs into hepatocytes was confirmed by albumin (ALB) immunocytochemistry. Results After addition of β-NGF, hepatocyte abstraction or co-culture with embryonic hepatic cells, the EGCs were differentiated into hepatocyte-like cells which were star-like or oval in shape. These hepatocyte-like cells ingested ICG and expressed ALB immunoreactivity. They could produce more ALB positive cells than control group (p<0.05), bFGF, EGF and RA had no such effects. Conclusion β-NGF, some unknown factors from hepatocyte abstraction and embryonic hepatic cells could induce EGCs differentiating into hepatocytes effectively.
	TO cite this article:FENG ZHI-HUI,LIU XIAO-PING,Guo Feifei. STUDY ON THE DIFFERENTIATION OF MOUSE EMBRYONIC GERM CELLS INTO HEPATOCYTES[OL].[ 2 April 2007] http://en.paper.edu.cn/en_releasepaper/content/11866

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	3. Real Time Detection of 1A-ARs Movement Stimulated by Phenylephrine in Single Living Cell
	Xu Ning ,Liang Zhangyi ,Xu Ming ,Guan YingHua ,He Qihua ,Han Qide ,Zhao Xinsheng ,Zhang Youyi
	Basic Medicine 17 November 2006
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	Abstract:AIM: To investigate the movement of 1A- adrenergic receptors( 1A-ARs) stimulated by agonist phenylephrine (PE) and the dynamics of receptors movement in real time in single living cells with millisecond resolution. METHODS: We labeled 1A-ARs using monoclonal anti-FLAG antibody and Cy3-conjugated Goat Anti-Mouse IgG and recorded the trajectory of their transport process in the living HEK293A cells stimulated by agonist phenylephrine (PE), then analysis the dynamic properties of them. RESULTS: The specific detection of 1A-ARs on the surface of living HEK293A- 1A cells was achieved. 1A-ARs internalize under stimulation of PE After the cells were stimulated with PE for 20 minutes, apparent co-localization was found between 1A-ARs and F-actins. And after 40 min stimulation of PE, trajectories of approximate linear motion in HEK293A- 1A cells were recorded, and the velocity of them were calculated. CONCLUSION: The specific labeling method on living cell surface provides a convenient means on real-time detection of the behavior of surface receptors. By this method we were able to specifically detect 1A-ARs, record the behavior of individual particles of receptors with 50ms exposure time in real time in single living cell.
	TO cite this article:Xu Ning ,Liang Zhangyi ,Xu Ming , et al. Real Time Detection of 1A-ARs Movement Stimulated by Phenylephrine in Single Living Cell[OL].[17 November 2006] http://en.paper.edu.cn/en_releasepaper/content/9683


	4. Genome restructuring for rebalancing in bacteria
	Liu Guirong,Liu Weiqiao,He Xiaoyan,Zhao Gexin,Xu Guomin,Li Junqian,Deng Juan,Tang Jie,Qi Danni,Zhang Fengmin,Liu Shulin
	Basic Medicine 14 November 2006
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	Abstract:Bacterial genome structure can be remarkably stable over extended periods of evolution, but may occasionally undergo a burst of rearrangement events. Evidence is accumulating in host-adapted Salmonella pathogens to demonstrate a correlation between genomic insertions and restructuring, supporting a hypothesis of genomic restructuring as a mechanism of topological rebalancing. These events may constitute a novel source of genomic variability that contributes to adaptive potential.
	TO cite this article:Liu Guirong,Liu Weiqiao,He Xiaoyan, et al. Genome restructuring for rebalancing in bacteria[OL].[14 November 2006] http://en.paper.edu.cn/en_releasepaper/content/9565


	5. Hyperactivated uterine NK cells contribute more for the regulation of murine pregnancy
	Zhang Jianhong,Sun Rui,Wei Haiming,Wu Dongmei,Tian Zhigang
	Basic Medicine 14 November 2006
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	Abstract:To understand the response of murine uterine natural killer (uNK) cells to Toll-like receptor (TLR) 3 agonist at the early stage of gestation, CBA×DBA/2 mice were intraperitoneally (i.p.) injected with polyinosinic-polycytidylic acid (poly I:C), the specific TLR-3 agonist, at dose of 10 μg/g·BW or PBS at gestation day (gd) 6.5. The percentage of intracellular TNF-α+ or IFN-γ+ uNK (DX5+CD3-) cells in the implantation sites of CBA×DBA/2 matings were significantly increased 24 hours after poly I:C injection. Surprisingly, poly I:C treatment significantly decreased the total number of uNK cells (either DX5+CD3- or DBA+) at fetal maternal surface, but had no influence on local NKT cells, T cells and DCs. This investigation will help to explain the central role for hyperactivated uNK cells in the progress of mice pregnancy.
	TO cite this article:Zhang Jianhong,Sun Rui,Wei Haiming, et al. Hyperactivated uterine NK cells contribute more for the regulation of murine pregnancy[OL].[14 November 2006] http://en.paper.edu.cn/en_releasepaper/content/9532


	6. Decidual TLR signal response associated with TNF-α regulate the uterine spiral artery transformation of CBA×DBA/2 mice
	Zhang Jianhong,Sun Rui,Wei Haiming,Wu Dongmei,Tian Zhigang
	Basic Medicine 14 November 2006
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	Abstract:The objective of this study was to examine the expression of Toll-like receptor (TLR) 3 at maternal fetal interface and determine whether exposure to the TLR3 agonist would induce innate immune response and trigger pregnancy loss. To address this, abortion-prone male DBA/2J mated-CBA/J female mice were given polyinosinic-polycytidylic acid (poly I:C, 10 μg/g·body weight, i.p.) or PBS at gestation day (gd) 6.5. All implantation sites appeared viable at gd 7.5 when endometrium was dissected for immunohistological examination. It was noted that poly I:C treatment increased fetal losses to 40.2±1.7% at midgestation stage, compared with control animals (11.0±3.0%). It was also observed that the ratio of vessel area to lumen area significantly increased at gd 10.5 and gd 12.5 after poly I:C treatment, indicating that the spiral arterial (SA) modification was impaired. Meanwhile, twenty-four hours after poly I:C injection, the expression of TLR3 dramatically elevated within decidua basalis (DB), and content of the endometrial TNF-α increased 2.7 fold but IFN-γ remained unchanged in homogenized endometrium. These results suggested that enhanced TNF-α expression in endometrial stroma may play a critical role in inflammatory factor production and the impairment of uterine spiral artery remodeling in the pregnancy failure of CBA×DBA/2 mating
	TO cite this article:Zhang Jianhong,Sun Rui,Wei Haiming, et al. Decidual TLR signal response associated with TNF-α regulate the uterine spiral artery transformation of CBA×DBA/2 mice[OL].[14 November 2006] http://en.paper.edu.cn/en_releasepaper/content/9512


	7. Internalization and distribution of three α1-adrenoceptor subtypes in HEK293A cells before and after agonist stimulation
	Wang Shuyi,Song Yao,Xu Ming,Han Qide,Zhang Youyi
	Basic Medicine 06 November 2006
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	Abstract:AIM: To examine the subcellular distribution of three α1-adrenoceptor (α1-AR) subtypes and their internalization and trafficking upon agonist stimulation in human embryonic kidney (HEK) 293 cells. METHODS: Confocal real-time imaging, ELISA and whole cell 3H-prazosin binding assay were applied to detect the distribution and localization of three α1-AR subtypes. RESULTS: α1A-AR was found both on the cell surface and in the cytoplasm; α1B-AR, however, was predominantly detected on the cell surface while α1D-AR was detected mainly in the intracellular compartments. After stimulation with phenylephrine, localization changes were detected by confocal microscopy for α1A- and α1B-ARs but localization of α1D-ARs was unaffected. Phenylephrine stimulation promoted a more rapid internalization of α1B-ARs than α1A-ARs. We were able to show α1D-AR internalization only when assayed by enzyme-linked immunosorbent assay (ELISA). Whole cell 3H-prazosin binding assay showed that α1A-AR functional receptors were detected both on the cell surface and in the cytoplasm; α1B-ARs, however, were detected predominantly on the cell surface while α1D-ARs were detected mainly intracellularly. Phenylephrine stimulation promoted internalization of α1A- and α1B-ARs. CONCLUSION: These results suggest that phenylephrine stimulation could induce changes in the localization of the three α1-ARs.
	TO cite this article:Wang Shuyi,Song Yao,Xu Ming, et al. Internalization and distribution of three α1-adrenoceptor subtypes in HEK293A cells before and after agonist stimulation[OL].[ 6 November 2006] http://en.paper.edu.cn/en_releasepaper/content/9309


	8. Image reconstruction using local inverse
	shuangren Zhao
	Basic Medicine 26 September 2006
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	Abstract:Truncated projections can arise from a detector with limited field of view (LFOV). Truncation artifacts can be reduced through extrapolation methods; however the reconstructed images are often over-corrected or under-corrected. Recently an iterative reconstruction-reprojection algorithm was developed, which incorporated extrapolation with iterative algorithm. It gave the possibility to better reduce truncation artifacts compared to using extrapolation method alone. This paper builds a theoretic foundation for the above iterative reconstruction-reprojection algorithm. The theoretic foundation is suitable to parallel-beam fan-beam cone-beam computed tomography(CT). Two pre-assumptions are summarized from CT system. A truncation-artifact-free solution for the problem of LFOV is derived from these assumptions. The solution contains a “local inverse” of matrix. The local inverse of a matrix consists of the general inverse of the sub-matrices of the matrix. The solution can be approximately implemented as the iterative reconstruction-reprojection algorithm mentioned above.
	TO cite this article:shuangren Zhao. Image reconstruction using local inverse[OL].[26 September 2006] http://en.paper.edu.cn/en_releasepaper/content/8513


	9. EEG characteristics in frequency domain in synaptic dysfunction rat model
	han li ying,tian xin
	Basic Medicine 02 August 2006
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	Abstract:The aim of this study is to investigate the EEG characteristics in frequency domain in frontal lobe, occipital lobe and hippocampus, i.e. cognition-related cortex of synaptic dysfunction model rat. Firstly, synaptic dysfunction model was made via microinjecting Aβ1-40 into hippocampal CA1 area of rat. Morris water maze behavioral test performed to investigate the learning and memory function of model group. Secondly, EEG in the above areaa for two groups were recorded. The spectrum for two groups was performed and the characteristics in frequency domain were analyzed. Results are that (1) The average escape latency in 3rd, 4th, 5th and 6th training times of model group are higher than that of normal. The average escape latency of normal group had marked different between 2nd and 5th training time, while that of model group contrast between 2nd and 7th training time (P<0.05). Secondly, without platform, the platform quadrant time percentage of model group was lower than the control (P<0.05). (2) In model rat, α rhythm was slowing down; α-band power of EEG decreased with peek frequency left shifted nearly 2Hz. And the power of δ-band and θ-band in frontal lobe, occipital and hippocampus all increased with different extent, from 20% to 2-fold. The synaptic dysfunction model rats were made successfully by microinjecting Aβ1-40 method. The model rats show the decreased learning and memory dysfunction. EEG frequency spectrum features in model rat show slower alpha rhythm (frequency depressing) with power amplitude lower or loss, slow waves (delta and theta wave) increasing with higher power amplitude. These can be consistent with the EEG of Alzheimer’s disease patients. Such provide electrophysiological basis for further plasticity and nerve regeneration study on the impaired cortex with synaptic dysfunction.
	TO cite this article:han li ying,tian xin. EEG characteristics in frequency domain in synaptic dysfunction rat model[OL].[ 2 August 2006] http://en.paper.edu.cn/en_releasepaper/content/7787


	10. A PCR-based Technology for Quickly Screening of gDNA Library
	Zhao Yongxiang
	Basic Medicine 03 July 2006
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	Abstract:Objective: To explore the feasibility of quickly screening of gDNA library with PCR technique. Methods: We adopted porcine α-1,3GT cDNA fragment as the probe, used the primers synthesized by the specific sequence on cDNA to carry out α-1,3GT gene screening of porcine gDNA library by combining PCR and in situ plaque hybridization, and then performed enzymic digestion, southern blot, sequencing and fluorescence in situ hybridization for location. Results: After having finished one-time hybridization and one-time PCR, we obtained 7 positive monoclones with very strong signals, and each insert length of them is over 8kb, including the third intron. Moreover, 3 tested clones among them contain the third and fourth exons according to the sequencing results, and FISH mapped the inserts of the 3 clones to pig chromosome 1q2.10-q2.11. Conclusion: PCR could be applicable to the quick screening of DNA library and much simpler than the conventional in situ plaque hybridization only used.
	TO cite this article:Zhao Yongxiang. A PCR-based Technology for Quickly Screening of gDNA Library[OL].[ 3 July 2006] http://en.paper.edu.cn/en_releasepaper/content/7438

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