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	1. Rapid and Sensitive Detection of Singapore grouper iridovirus by Loop-Mediated Isothermal Amplification
	Mao Xinliang,Zhou Sheng,Xu Dan,Gong Jie,Cui Huachun,Qin Qiwei ,Qin Qiwei
	Biology 24 December 2007
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	Abstract:Aims: The aim of this paper is to develop a loop-mediated isothermal amplification (LAMP) method for rapid, sensitive and inexpensive detection of SGIV in grouper, Epinephelus sp. Methods and Results: A set of six specific primers was designed by targeting the SGIV ORF-014L. With Bst DNA polymerase large fragment, the target DNA can be amplified as early as 20 min at 65°C in a simple water bath. The detection limit is about 0.02 fg (equivalent to 6.3 copies) of plasmid ORF-014L. LAMP products could be judged with three different methods. There were no cross reactions with 7 other aquatic animal viruses indicating high specificity of the LAMP. The LAMP method was applied to detect SGIV in virus infected GP cells and grouper tissues effectively. Conclusions: The LAMP described in this study is a cheap, sensitive, specific and rapid protocol for detection of SGIV in cells and in grouper tissues. Significance and Impact of the Study: The developed LAMP method can be simply applied both in field condition and in laboratory operation for specific detection of SGIV infection.
	TO cite this article:Mao Xinliang,Zhou Sheng,Xu Dan, et al. Rapid and Sensitive Detection of Singapore grouper iridovirus by Loop-Mediated Isothermal Amplification[OL].[24 December 2007] http://en.paper.edu.cn/en_releasepaper/content/17288


	2. Effects of chronic morphine exposure on visual response variability and latency in cats
	Zaiyang Long,Zhen Liang,Lihua He
	Biology 17 December 2007
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	Abstract:Chronic morphine exposure results in a degradation of the functional properties of cortical neurons. However, little evidence has been shown about morphine’s effects on the variability of visual response. To investigate whether chronic morphine exposure influences variability, we compared neurons of lateral geniculate nucleus (LGN) in morphine- and saline-treated cats. We found LGN neurons exhibited significantly increased variability after morphine administration. It is known that variability might act as one of the factors affecting response latency. Therefore, we correlated increased variability and prolonged latency, and this study showed the change of variability might contribute to delayed response accompanied by morphine. Increased variability together with prolonged latency should contribute to functional degradation in cortical areas after chronic morphine exposure.
	TO cite this article:Zaiyang Long,Zhen Liang,Lihua He. Effects of chronic morphine exposure on visual response variability and latency in cats[OL].[17 December 2007] http://en.paper.edu.cn/en_releasepaper/content/17066

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	3. Isolation of Trichoderma reesei PyrG negative mutant by UV Mutagenesis and its Application in Transformation
	Long Hao,Wang Tianhong,Zhang Yingkuan
	Biology 17 December 2007
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	Abstract:Two uridine auxotrophic mutants of Trichoderma reesei were isolated by resistance to 5-fluoroorotic acid after UV mutagenesis. One mutant, called M23, was complemented with the Aspergillus niger pyrG gene carried by plasmid pAB4-1. Mutated pyrG gene of M23 was cloned and DNA sequencing analysis indicated that a cytosine inserted into the 934-939 oligo dC position of the pyrG coding region, resulting in a frameshift mutation. Transformation efficiency was approximately 200-300 transformants per μg of DNA with plasmid pAB4-1. Stable transformants were obtained by monosporic culture and showed to be prototroph after successive propagation. Vitreoscilla hemoglobin expression plasmid pUCVHb co-transformed with plasmid pAB4-1 attained transformation efficiency of 71.8% versus 21.6% with pAN7-1. Southern blot analysis of the transformants demonstrated that plasmid pUCVHb was integrated into the chromosomal DNA. The experimental results demonstrated that the pyrG-based system was more efficient and timesaving than the conventional hygromycin B resistance-based transformation system.
	TO cite this article:Long Hao,Wang Tianhong,Zhang Yingkuan. Isolation of Trichoderma reesei PyrG negative mutant by UV Mutagenesis and its Application in Transformation[OL].[17 December 2007] http://en.paper.edu.cn/en_releasepaper/content/17054


	4. A new insight into the species barrier of Mammalian Prions obtained from structure-based conservation analysis
	Yaofeng Wang,Bingjie Hu,Yuan Liu,Huili Zhang,Youtao Song
	Biology 17 December 2007
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	Abstract:In the previous study done by Scott, M.R. et al, residues 184, 203 and 205 were predicted to contribute to an epitope modulating prion transmission, however, the evolutionary features of these residues were still unknown. To explore that, the functional domains (121-231) of 8 mammalian prions were examined by an informative and sensitive structural conservation analysis in this study. The results identified the three residues as highly structure-conserved but sequence-nonconserved. This suggested us the residues with these features may play an important role in modulating the transmission of prion diseases. Based on our hypothesis, residue 215 with the same structure feature was also predicted to involve in prion transmission. Subsequently, a phylogenetic tree was reconstructed. The topology of the tree can explain the mystery of species barrier occurred between the mammalian prions which cannot be deduced by simply sequence phylogenetic tree.
	TO cite this article:Yaofeng Wang,Bingjie Hu,Yuan Liu, et al. A new insight into the species barrier of Mammalian Prions obtained from structure-based conservation analysis[OL].[17 December 2007] http://en.paper.edu.cn/en_releasepaper/content/17036


	5. Effect of Calnexin Deletion on the Expession Level of Binding Protein (BiP) Under Heat Stress Conditions in Saccharomyces cerevisiae
	Zhang Huili ,Hu Bingjie ,Ji Yanyan ,Akio Kato,Song Youtao
	Biology 14 December 2007
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	Abstract:In order to investigate the effect of calnexin deletion on the induction of main ER molecular chaperone BiP, we cultured the wild-type and calnexin-disrupted Saccharomyces cerevisiae strains under the normal and stress conditions. The growth rate of the calnexin-disrupted yeast was almost the same as that of the wild-type yeast under those conditions. However, the induced mRNA level of BiP in the ER was evidently higher in calnexin-disrupted S. cerevisiae relative to the wild type at 37 C, but was almost the same in the two strains under the normal conditions. The western blotting analysis for BiP protein expression in the ER yielded results that showed a parallel in their mRNA levels in both strains. It is suggested that under heat stress conditions the induction of BiP in the ER could recover parts of the function of calnexin in calnexin-disrupted yeast, and result in the same growth rate as in wild-type yeast.
	TO cite this article:Zhang Huili ,Hu Bingjie ,Ji Yanyan , et al. Effect of Calnexin Deletion on the Expession Level of Binding Protein (BiP) Under Heat Stress Conditions in Saccharomyces cerevisiae[OL].[14 December 2007] http://en.paper.edu.cn/en_releasepaper/content/16921


	6. The assembly of a novel enzyme biosensor for aflatoxin B1 detection
	Liu Daling,shen Yi,Yao Dongsheng,Zhang Jing
	Biology 13 November 2007
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	Abstract:A novel biosensor for aflatoxin B1 detecting has been reported in this paper. The biosensor electrode for AFB1 detecting was assembled by immobilized aflatoxin-oxidoreductase using open-ended multi-walled carbon nanotubes as matrix. Its linear range was between 0.16uM and 3.2uM. And if the specific anti-aflatoxin B1 antibody and aflatoxin oxidoreductase were both immobilized on the electrode with Multi-Walled carbon nanotubes, the detection limit of the modified electrode could be 16 nM with a 10 times improved sensitivity. The aflatoxin enzyme biosensor assembled this way strode one step forward its practical application.
	TO cite this article:Liu Daling,shen Yi,Yao Dongsheng, et al. The assembly of a novel enzyme biosensor for aflatoxin B1 detection[OL].[13 November 2007] http://en.paper.edu.cn/en_releasepaper/content/16323


	7. The positive influences of Mg2+, Mn2+and Zn2+ addition on the gamma-CGTase Production by Bucillus macorous
	Wang feng,Du guocheng,Cheng jian
	Biology 25 October 2007
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	Abstract:The effects of Mg2+, Mn2+ or Zn2+ addition on gamma-CGTase production by Bacillus macorous WSH02-06 were studied. The gamma-CGTase yield was increased maximally almost three-times by the addition of Mg2+, Mn2+ and Zn2+. The addition of these cations also stimulated cell growth. The consumption of Mg2+ was promoted in the cultures with the co-existance of Mg2+ with Mn2+ or/and Zn2+. The maximal gamma-CGTase yield and dry cell weight showed a linear increasing relation with the consumption of Mg2+. The increased intracellular concentration of ATP and ATP-to-ADP ratios by the addition of these cations can provide more energy for the better cell growth and higher gamma-CGTTase production.
	TO cite this article:Wang feng,Du guocheng,Cheng jian. The positive influences of Mg2+, Mn2+and Zn2+ addition on the gamma-CGTase Production by Bucillus macorous[OL].[25 October 2007] http://en.paper.edu.cn/en_releasepaper/content/15921


	8. EFFECTS OF GLYCEROL PRETREATMENT ON RECOVERY AND ANTIOXIDANT ENZYME ACTIVITIES OF LYOPHILIZED RED BLOOD CELLS
	Zhou Xinli ,He Hui ,Liu Baolin ,Hua ZeChao ,Chen Ying
	Biology 29 September 2007
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	Abstract:Successful storage of red blood cells (RBCs) by freeze-drying technique has important implications in blood transfusion and clinical medicine. We presented a method of preservation RBCs by pretreating them with glycerol solution and then freeze-drying. The effects of glycerol pretreatment on recovery and antioxidant enzyme activities of lyophilized RBCs were investigated. RBCs pretreated in 40% glycerol resulted in 55.3±4.26% numerical recovery and 53.5±3.85% hemoglobin recovery, which were significantly different from control group (P<0.01). Superoxide dismutase(SOD), catalase (CAT) and glutathione peroxidase (GSH-PX) activities of 40% glycerol pretreated, freeze-dried RBCs almost remained fully active after freeze-drying. After 180 days storage, they decreased by 25.6%，21.5% and 18.4%, respectively, which are significantly lower than those of no glycerol pretreated, freeze-dried RBCs (P<0.1). These data demonstrated that glycerol pretreatment had beneficial effects on the recovery and antioxidant enzyme activities of lyophilized RBCs.
	TO cite this article:Zhou Xinli ,He Hui ,Liu Baolin , et al. EFFECTS OF GLYCEROL PRETREATMENT ON RECOVERY AND ANTIOXIDANT ENZYME ACTIVITIES OF LYOPHILIZED RED BLOOD CELLS[OL].[29 September 2007] http://en.paper.edu.cn/en_releasepaper/content/15435


	9. Observation and Quantitative Compare of P-like cells in Right Atrium between Male and Female Mice
	Yuan Guoqiang,Bai Zhongtian,Wang Jianlin
	Biology 06 September 2007
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	Abstract:Aim To investigate the morphological basis on which sex differences in the probability of happening atrial fibrillations by observing morphologically and comparing quantitatively P-like cells in the right atrium between male and female mice. Methods 20 adult healthy Wistar mice ware used in the experiment， male and female mice ten respectively. Wright atrias of this mouse were dissected. Serial sections from this tissue were stained with hematoxylin and eosin. Then slides were examined， measured and photographed by light microscope. Results It was indicated that cell acreage， nuclear acreage and the ratio between them of P-like cells in the right atrium from male and female were different. Conclusion The morphological differences of P-like cells in the right atrium from male and female may be the reason that leaded to sex differences in happening atrial fibrillations.
	TO cite this article:Yuan Guoqiang,Bai Zhongtian,Wang Jianlin. Observation and Quantitative Compare of P-like cells in Right Atrium between Male and Female Mice[OL].[ 6 September 2007] http://en.paper.edu.cn/en_releasepaper/content/14918


	10. Development of Trichoderma reesei cbh1 promoter to improve heterologous gene expression
	Ti Liu ,Tianhong Wang,Xian Li,Xuan Liu
	Biology 30 August 2007
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	Abstract:To improve heterologous gene expression in Trichoderma reesei, a set of optimal artificial cbh1 promoters were obtained. The -677～-724 region with three potential glucose repressor binding sites was deleted. And then the -620～-820 region of the modified cbh1 promoter including the CCAAT box and Ace2 binding site was repeatedly inserted into the modified cbh1 promoter, obtaining promoters with copy number 2, 4 and 6, respectively. The experimental results showed that the glucose repression effects were abolished and the glucuronidase (GUS) reporter gene expression guided by these multi-copy promoters was markedly enhanced as copy number increased simultaneously. The date showed the great promise of utilizing promoter artificial modification strategy to increase heterologous gene expression in filamentous fungi and provided a set of optional high-expression vectors for gene function investigation and strain modification.
	TO cite this article:Ti Liu ,Tianhong Wang,Xian Li, et al. Development of Trichoderma reesei cbh1 promoter to improve heterologous gene expression [OL].[30 August 2007] http://en.paper.edu.cn/en_releasepaper/content/14772

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