Abstract:Fetal origin of adult disease has gained lots of attention in relation to the occurrence of insulin resistance. Studys found offspring of pregnant rats tested positive for angiotensin II type 1 receptor autoantibody (AT1-AA) exhibited both liver damage and systemic insulin resistance during adulthood. But the mechanism and time-course associated with symptom remain unclear. Normal pregnant rats were administered with preeclampsia serum-derived AT1-AA in the second trimester to establish AT1-AA positive pregnant rat models. Compared to saline group, fasting serum glucose and insulin levels, insulin resistance index values were higher, and impaired glucose tolerance, abnormal insulin tolerance, islet compensatory hypertrophy were observed in adolescent and middle-aged offspring of AT1-AA group. Triglyceride and systolic blood pressure levels were elevated in adolescence. Hepatic glycogen synthetase reduced in the third trimester, adolescence and middle age. Expression of insulin receptor subunit, insulin receptor substrate 1/2, and their phosphoprotein decreased in hepatic insulin signaling pathway of adolescent and middle-aged offspring of AT1-AA group. We found the offspring of AT1-AA positive pregnant rats exist insulin resistance in adolescence. Meanwhile, hepatic insulin receptor and downstream receptor pathway disorder may be an important mechanism of insulin resistance in AT1-AA positive pregnant rat offspring.

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	1. Fetal exposure to angiotensin II type 1 autoantibody induces hepatic insulin resistance in the adolescent offspring of rats
	WEI Mingming,ZHANG Suli,YANG Xiaoli,WANG Li,ZHAO Chengrui,LEI Jinghui,WANG Pengli,LIU Huirong
	Basic Medicine 09 February 2017
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	Abstract:Fetal origin of adult disease has gained lots of attention in relation to the occurrence of insulin resistance. Studys found offspring of pregnant rats tested positive for angiotensin II type 1 receptor autoantibody (AT1-AA) exhibited both liver damage and systemic insulin resistance during adulthood. But the mechanism and time-course associated with symptom remain unclear. Normal pregnant rats were administered with preeclampsia serum-derived AT1-AA in the second trimester to establish AT1-AA positive pregnant rat models. Compared to saline group, fasting serum glucose and insulin levels, insulin resistance index values were higher, and impaired glucose tolerance, abnormal insulin tolerance, islet compensatory hypertrophy were observed in adolescent and middle-aged offspring of AT1-AA group. Triglyceride and systolic blood pressure levels were elevated in adolescence. Hepatic glycogen synthetase reduced in the third trimester, adolescence and middle age. Expression of insulin receptor subunit, insulin receptor substrate 1/2, and their phosphoprotein decreased in hepatic insulin signaling pathway of adolescent and middle-aged offspring of AT1-AA group. We found the offspring of AT1-AA positive pregnant rats exist insulin resistance in adolescence. Meanwhile, hepatic insulin receptor and downstream receptor pathway disorder may be an important mechanism of insulin resistance in AT1-AA positive pregnant rat offspring.
	TO cite this article:WEI Mingming,ZHANG Suli,YANG Xiaoli, et al. Fetal exposure to angiotensin II type 1 autoantibody induces hepatic insulin resistance in the adolescent offspring of rats[OL].[ 9 February 2017] http://en.paper.edu.cn/en_releasepaper/content/4718781


	2. The involvement of sirtuins during optic nerve injury of rats
	MENG Pei,WEI Jiacong,LIANG Jiajian,WANG Jingying,ZHI Ye,CUI Qi,GENG Yiqun
	Basic Medicine 04 January 2016
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	Abstract:Sirtuins protects cells from injury while 7 members may have different roles. In this study, we applied young rat optic nerve injury model to analyze the change of Sirt1-7 at different time point to better understand the role of Sirtuins during optic nerve injury. 12 week old adult male F344 rats were used (total n=36). Rats were divided into two groups randomly. One group underwent optic nerve cut and the other group underwent peripheral nerve-optic nerve graft (PN-ON graft) on the left eye. At the time point of 1 day, 3 day, 1 week, 2 week and 4 week, the rats were sacrificed. Retinas of both eyes were removed. Total RNA was extracted and first-strand cDNA was synthesized. Sirt1-7 and housekeeping β-actin quantitative real-time PCR was performed. The quantitative real time PCR profile showed that 7 members of Sirtuins of both groups had time period after surgery. Sirtuin family mRNA transcript levels increased following optic nerve injury with and without peripheral nerve grafting. Sirt1 showed a quite different transcription pattern from the rest of the members. Our data indicated that Sirt1 and Sirts 2-7, or just Sirt2, played opposing roles in optic nerve injury. Sirts 4 and 6 were the only Sirts higher in the PN-graft group, where neuronal survival should be higher, these results suggested that Sirts 4 and 6 played the predominant role for Sirts in neuroprotection or axon regeneration.
	TO cite this article:MENG Pei,WEI Jiacong,LIANG Jiajian, et al. The involvement of sirtuins during optic nerve injury of rats[J].

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	3. Influences of Angiotensin I-Converting Enzyme and Endothelial Nitric Oxide Synthase Gene Polymorphisms on Hepatocellular Carcinoma Risks in China
	YUAN Fang,ZHANG Lushun,LI Hongyu,LIAO Miao,LV Meili※,ZHANG Chongjie※
	Basic Medicine 11 January 2013
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	Abstract:Aims: Growing evidences suggested that angiotensin-converting enzyme (ACE) gene and endothelial nitric oxide synthase (eNOS) gene have been associated with the risk in a wide range of cancers. The objective of this study was to examine whether two DNA polymorphisms at the ACE insertion/deletion (I/D) and NOS intron 4 variable number of tandem repeats (4a/4b) have been linked with the risk of developing hepatocellular carcinoma (HCC) in a Chinese population. Methods: Polymorphisms of ACE I/D and eNOS 4a/4b were genotyped in 293 HCC patients and 384 healthy control subjects using the polymerase chain reaction (PCR). Results: Frequencies of D allele and DD genotype in ACE gene of HCC patients were significantly different from that in healthy controls. However, no differences were observed in eNOS 4a/4b genotype and allele frequencies between the HCC and controls. Conclusions: These findings indicate that the ACE I/D polymorphism may play a role in HCC progression.
	TO cite this article:YUAN Fang,ZHANG Lushun,LI Hongyu, et al. Influences of Angiotensin I-Converting Enzyme and Endothelial Nitric Oxide Synthase Gene Polymorphisms on Hepatocellular Carcinoma Risks in China[OL].[11 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4511647


	4. Ochratoxin A-induced G2 phase arrest in human gastric epithelium cells via p38 MAPK signaling pathway
	WANG Yuan,LIU Jing,CUI Jinfeng,XING Lingxiao,WANG Junling,YAN Xia,ZHANG Xianghong
	Basic Medicine 05 August 2012
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	Abstract:Objecitve: Ochratoxin A (OTA) is a naturally occurring mycotoxin, our previous study demonstrated that OTA evoked cell cycle arrest at G2 phase in GES-1 cells. The aim of the present study was to explore the putative role of p38 MAPK signaling pathway in OTA-induced G2 phase arrest in human gastric epithelium cells (GES-1) in vitro. Methods: After treated with different concentrations of OTA (0, 5, 10 and 20 μM) for 24 h, the expression of p38/Phospho-p38 at protein level in GES-1 cells was determined by Western blot. GES-1 cells were then preincubated with SB203580 (10 μM) for 30 min or reversely transfected with siRNA targeting p38, then the distribution of cell cycle phases was detected by Flow cytometry, and the expression of Cdc25C/p-Cdc25C, Cdc2/p-Cdc2, CyclinB1 and complex of Cdc2-CyclinB1 were detected by Western blot analysis and Immunoprecipitation. Results: OTA at different concentrations of 5, 10 and 20 μM for 24 h could activate p38 MAPK signaling pathway of GES-1 cells. p38 specific inhibitor (SB203580) and p38 siRNA transfaction blocked the down-regulation of Cdc25C/p-Cdc25C, Cdc2/p-Cdc2, CyclinB1 and complex of Cdc2-CyclinB1, and then abolished the G2 arrest induced by OTA. Conclusion: p38 MAPK signaling pathway was involved in OTA-induced G2 arrest in GES-1 cells by modulating G2 phase related factors.
	TO cite this article:WANG Yuan,LIU Jing,CUI Jinfeng, et al. Ochratoxin A-induced G2 phase arrest in human gastric epithelium cells via p38 MAPK signaling pathway[OL].[ 5 August 2012] http://en.paper.edu.cn/en_releasepaper/content/4486367


	5. Expression of calponin h2 in anti-Thy-1.1 nephritis: negative correlation with TGF beta1 expression
	LI Hui
	Basic Medicine 08 February 2012
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	Abstract:BACKGROUND: Evidence implied that calponin h2 (CNh2), an actin-binding protein, may be an antiproliferative gene. However, the expression of CNh2 in anti Thy1.1 glomerulonephritis (ATG) has not yet become clear. In this study, we sought to assess the relationship between CNh2 and transforming growth factor beta 1(TGF-β1) expression in vitro and in vivo. METHODS: In vivo study, ATG rats were generated and expression of CNh2 and TGF-β1 were assessed by immunohistochemistry, reverse transcription polymerase chain reaction (RT-PCR) and western blot, respectively. In vitro study, cultured mesangial cells(MsC) were stimulated with 2.08mmol/L argrinine or 200mmol/L ethanol for 48 hour, then expression of CNh2 and TGF-β1 were evaluated at the mRNA and protein level. RESULTS: CNh2 expression enhanced from day 3 to day 14, whereas TGF-β1 expression greatly increased from day 7 to day 28 in ATG (P<0.05). Up-regulation of TGF-β1expression and down-regulation of CNh2 expression were observed in MsC incubated with argrinine, whereas increased TGF-β1 expression and decreased CNh2 expression were shown in MsC treated with ethanol. CONCLUSIONS: CNh2 expression was enhanced in the early stage of ATG. With the progression of ATG, CNh2 expression decreased accompanied by an evident increase in TGF-β1 expression. CNh2 may function as a negative feedback regulator of TGF-β1 expression in rat MsC.
	TO cite this article:LI Hui. Expression of calponin h2 in anti-Thy-1.1 nephritis: negative correlation with TGF beta1 expression[OL].[ 8 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4465455


	6. The research about REIC expression and its correlation with clinicopathological parameters of gastric carcinomas
	Xu Xiaoyan,Xia Pu,Zheng Huachuan
	Basic Medicine 27 October 2011
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	Abstract:REIC is down-regulated in immortalized cell lines compared with the parental normal counterparts. It may inhibit colony formation, tumor growth, and induce the apoptosis. Here, REIC expression was examined in gastric carcinomas by RT-PCR, Western blot and immunohistochemistry, and compared with clinicopathological parameters of carcinoma. Immunohistochemically, REIC expression was negatively associated with tumor size, lymph node metastasis, dedifferentiation or poor prognosis of carcinoma. These results suggest that REIC expression may be a promising objective and effective marker to predict pathobiological behaviors and prognosis of gastric carcinoma.
	TO cite this article:Xu Xiaoyan,Xia Pu,Zheng Huachuan. The research about REIC expression and its correlation with clinicopathological parameters of gastric carcinomas[OL].[27 October 2011] http://en.paper.edu.cn/en_releasepaper/content/4447599


	7. More mesenchymal stem cells are recovered from bone marrow aspirates by culturing bone marrow particles and mononuclear cells respectively
	Xing Wen,Liu Pengxia,Liu Meng,Yang Shaoguang,Zhao Qinjun,Li Jianping,Lu Shihong,Ren Hongying,Han Zhongchao
	Basic Medicine 04 January 2011
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	Abstract:Bone marrow (BM) is the major source of mesenchymal stem cells (MSCs). In most experiments, MSCs were classically cultured from mononuclear cells (MNCs) isolated by density gradient centrifugation method. However, several groups have demonstrated that this method was less efficient for MSCs' recovery. Here, we investigated whether BM particles were the cause and how to isolate them. A total of 20 patients were enrolled. MNCs were cultured by standard adherence and BM particles were cultivated by primary explant culture. For BM from patients 1-10, we first isolated MNCs, then filtered out BM particles. We then compared the morphology and the fibroblastic colony number between cultures of MNCs and BM particles. For BM from patients 11-20, we processed them in opposite order. We then compared the immunophenotype and function between adherent cells expanded from MNCs and BM particles. In addition, for patients 11-20，we cultured the left BM aspirates after BM particles and MNCs were isolated respectively. Adherent cells from BM particles were MSCs. After BM particles were filtered out and cultured separately, MSCs could be recovered completely from MNCs isolated by density gradient centrifugation and no MSCs were left in the residual BM aspirates. BM particles, which have been mostly discarded by the method of density gradient centrifugation, are another important source of MSCs and they can be cultivated reliably by primary explant culture. More MSCs are recovered from a single BM sample by culturing BM particles and MNCs respectively.
	TO cite this article:Xing Wen,Liu Pengxia,Liu Meng, et al. More mesenchymal stem cells are recovered from bone marrow aspirates by culturing bone marrow particles and mononuclear cells respectively[J].


	8. The correlation of fascin over-expression with motility and invasion of tumor cells
	Li Weiping,Ma Gui
	Basic Medicine 12 November 2010
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	Abstract:In the progression of the cancer invasion, carcinoma cells migrate away from primary tumors, traverse to basement membrane and extracellular matrix (ECM), overcome the cell-cell and cell-ECM adhesions, invade surrounding tissue, and come into the metastases through lymphatic and/or blood system .Of all these processes, the acquisition and increase of cell motile and invasive properties play a crucial role in the neoplasm progression. Fascin, an Actin-bundling protein, is considered to induce specific morphological features of membrane protrusion, as well as the changes of cell adhesion and cell interactions, and ultimately enhance the cell motility for subsequent invasion and metastasis, which is significantly negatively correlated with prognosis and survival rate. It is profound to study the involvement of fascin in the mechanism of invasion and metastasis so as to provide effective early diagnosis for potentially aggressive tumors or be used to develop therapeutic strategies that can arrest invasion and even metastases.
	TO cite this article:Li Weiping,Ma Gui. The correlation of fascin over-expression with motility and invasion of tumor cells[OL].[12 November 2010] http://en.paper.edu.cn/en_releasepaper/content/4391408


	9. Dynamic urinary proteomic analysis
	Sun Wei ,Chen Yong ,Gao Youhe
	Basic Medicine 24 March 2009
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	Abstract:Human urinary proteome analysis is a convenient and efficient approach for understanding disease processes affecting the kidney and urogenital tract. Many potential biomarkers have been identified in previous differential analyses; however, dynamic variations of the urinary proteome have not been intensively studied, and it is difficult to conclude that potential biomarkers are genuinely associated with disease rather then simply being physiological proteome variations. In this paper, pooled and individual urine samples were used to analyze dynamic variations in the urinary proteome. Five types of pooled samples (first morning void, second morning void, excessive water-drinking void, random void, and 24 h void) collected in 1 day from six volunteers were used to analyze intra-day variations. Six pairs of first morning voids collected a week apart were used to study inter-day, inter-individual, and inter-gender variations. The intra-day, inter-day, inter-individual, and inter-gender variation analyses showed that many proteins were constantly present with relatively stable abundances, and some of these had earlier been reported as potential disease biomarkers. In terms of sensitivity, the main components of the five intra-day urinary proteomes were similar. The advantages and disadvantages of pooling samples are also discussed.
	TO cite this article:Sun Wei ,Chen Yong ,Gao Youhe . Dynamic urinary proteomic analysis [OL].[24 March 2009] http://en.paper.edu.cn/en_releasepaper/content/30668

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