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	1. Effects of Down-regulated miR-143 on Human Bronchial Epithelial Cells Exposed to Benzo[a]pyrene
	Wang Xikai,Zhang Yanqiu,Pu Yuepu,Yin Lihong,Liang Geyu
	Preventive Medicine and Hygienics 08 January 2011
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	Abstract:MicroRNAs(miRNAs) are small non-coding RNAs, which have the association with the occurrence or the development of the cancer. In this study, we investigated the effect of the down-regulated miR-143 on human bronchial epithelial cells (16-HBE) exposed to Benzo[a]pyrene [B(a)P]. Anti-miR-143 was transfected into cells to down-regulate the expression of miR-143. Cell proliferation, apoptosis and cycle of transfected cells were detected by methyl thiazolyl tetrazolium (MTT) assay and flow cytometry after 24 h exposed of B(a)P, respectively. Our results confirmed that B(a)P had significant toxic effect on the cell proliferation, raised the apoptosis rate and blocked more cells at the S period in cell cycle (P<0.05). However, there was no significant difference between transfected group and non-transfected group after cells exposed to B(a)P (P>0.05). The results suggested that down-regulated miR-143 has no significant influence on cellar bio-effects induced by B(a)P under the present experimental conditions.
	TO cite this article:Wang Xikai,Zhang Yanqiu,Pu Yuepu, et al. Effects of Down-regulated miR-143 on Human Bronchial Epithelial Cells Exposed to Benzo[a]pyrene[OL].[ 8 January 2011] http://en.paper.edu.cn/en_releasepaper/content/4402435


	2. Effects of BmKNJX11, a bioactive polypeptide purified from Buthus martensi Karsch, on TTX-R sodium channels in rat dorsal root ganglion neurons
	Xijie Wang,Peng Xu,Shanshan An,Qin Sun,Jie Cheng,Rong Gao,Hang Xiao
	Preventive Medicine and Hygienics 28 July 2009
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	Abstract:BmKNJX11is a 7036.85 Da polypeptide purified from the venom of Asian scorpion Buthus martensi Karsch (BmK). With whole cell recording, BmKNJX11 inhibited tetrodotoxin- resistant voltage-gated sodium channels (TTX-R VGSC) in freshly isolated rat dorsal root ganglion (DRG) neurons in a concentration- and voltage-dependent manner. At a concentration of 40 μg/ml BmKNJX11 increased the activation threshold and produced positive shifting of TTX-R sodium current (INa) activation curve. In addition, BmKNJX11 induced shifting of the steady-state inactivation curve to left, delayed the recovery of TTX-R INa from inactivation, and also reduced the fraction of available sodium channels. These results suggested that BmKNJX11 might exert effects on VGSC by binding to a specific site. Considering that TTX-R VGSC expressed in DRG neurons play a critical role in nociceptive transmission, the interaction of BmKNJX11 with TTX-R VGSC might lead to a change in excitability of nociceptive afferent fibers which may be involved in the observed peripheral pain expression.
	TO cite this article:Xijie Wang,Peng Xu,Shanshan An, et al. Effects of BmKNJX11, a bioactive polypeptide purified from Buthus martensi Karsch, on TTX-R sodium channels in rat dorsal root ganglion neurons[OL].[28 July 2009] http://en.paper.edu.cn/en_releasepaper/content/34118


	3. Interaction of calcium intake and osteoprotegerin gene polymorphisms on bone mass in premenarche girls
	He Guo-peng,Chen Yuming,Li Xing,Su Yixiang
	Preventive Medicine and Hygienics 19 January 2009
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	Abstract:To investigate the interaction effects of promoter region A163G,T245G and T950C polymorphisms of osteoprotegerin(OPG) gene and calcium intake on bone mass in premenarche girls. Methods: The OPG genotypes were determined by PCR-RFLP in 214 premenarche girls of Han race in Guangzhou area, and bone mineral density(BMD) at lumbar spine(L1-4), femoral neck, Ward’s triangle area(WSBMD) and total BMD, were measured by dual-energy X-rayabsorptiometry(DEXA). Current dietary calcium intakes were assessed by 3-day food record questionnaire and 3-day weighed food record method. Results: Hardy-Weinberg equilibrium was evident for OPG polymorphisms. After adjusting the confounding factors of year, height and weight, ANCOVA showed significant effect of interaction of calcium intake and T950C SNPs on WSBMD(P=0.046). WSBMD of subjects in calcium intake<500mg/d group was lower 13.0% 、23.7% than those in 500~800mg/d group and >800 mg/d group respectively among girls with TT genotype of T950C locus. However, such significance was not detected among girls with TC or CC genotype of T950C locus. Significant interaction was not observed between A163G、T245G SNPs and calcium intake on BMD. Conclusion: calcium intake and OPG T950C SNP has significant interaction on WSBMD in premenarche girls. More calcium intake would be benefit to gain higher WSBMD for girls with TT genotype of T950C locus than those with TC or CC genotype
	TO cite this article:He Guo-peng,Chen Yuming,Li Xing, et al. Interaction of calcium intake and osteoprotegerin gene polymorphisms on bone mass in premenarche girls[OL].[19 January 2009] http://en.paper.edu.cn/en_releasepaper/content/28070


	4. Up-regulation of RAGE and S100A6 in rats exposed to cigarette smoke
	Zhang Su-ping,Wu Yan-wen,Wu Zhao-zhao,Hai-Yun Liu,Ji-Hua Nie,Tong Jian
	Preventive Medicine and Hygienics 08 January 2009
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	Abstract:Cigarette smoke has been widely investigated in terms of epidemiological and pathological observation in relation to human lung diseases. In this study, we established an animal model exposed to cigarette smoke and looked for the potential molecular mechanisms at the protein level. Wistar rats were exposed to cigarette smoke at concentrations of 20% and 60% as the low and high concentration group. The exposure was conducted twice a day、5 days a week for 43 weeks. As a major metabolite of nicotine in cigarette, cotinine in rat urine was determined by LC-MS. A time-and dose-dependent analysis indicated that the cotinine level may be used as a biomarker of smoke exposure. Expression of receptor for advanced glycation endproducts(RAGE), an immunoglobulin superfamily that triggers the intracellular signal cascade reaction leading to inflammation and its ligand S100A6(calgranulin) in bronchial epithelial cells and lung tissues of rats exposed to cigarette smoke, were found to be correlated with the cotinine level, indicating that RAGE and S100A6 may attribute to the inflammation and oxidative damage caused by cigarette smoke.
	TO cite this article:Zhang Su-ping,Wu Yan-wen,Wu Zhao-zhao, et al. Up-regulation of RAGE and S100A6 in rats exposed to cigarette smoke[OL].[ 8 January 2009] http://en.paper.edu.cn/en_releasepaper/content/27495


	5. Surface Sterilization and Modification of Medical PTFE by Remote Argon Plasma
	Hongxia Liu,Jierong Chen
	Preventive Medicine and Hygienics 19 June 2007
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	Abstract:This paper studies remote argon plasma sterilization to medical Poly(tetrafluoroethylene)(PTFE) film surface contaminated with E.coli and characterized surface structure, performances of PTFE film by the water contact angle, mass loss and platelet adhesion measurements as well as X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). Results show that both remote and direct argon plasmas can sterilize effectively (GE ≥3.769) at the conditions of RF power 100W, exposure time 120s and argon flux 20 cm3/min. But the remote argon plasma sterilization contributes more effectively to the defluorination(F/C = 2.2425) from the PTFE film surface than that of the direct one contributes(F/C = 2.4853) and introduces more oxygen-containing groups (e. g. C=O) into it, makes this surface higher hydrophilicity and better antithrombogenicity while degradation and damages least, biocompatibility most excellent. Both the optimal inactivation and surface performances of PTFE film for use in medicine can be obtained simultaneously by remote argon plasma sterilization. The essential reason is that remote argon plasma sterilization can enhance interaction reactions with argon radicals relative to those with electron and argon ions.
	TO cite this article:Hongxia Liu,Jierong Chen. Surface Sterilization and Modification of Medical PTFE by Remote Argon Plasma[OL].[19 June 2007] http://en.paper.edu.cn/en_releasepaper/content/13570


	6. IMMUNOPROTECTION AND DIAGNOSTIC POTENTIAL OF SIGNALING PROTEIN 14-3-3 OF SCHISTOSOMA JAPONICUM×
	Liu Qingzhong,Shen Jilong,Li Defa,Li Feng,Wang Xuelong,JIang Baoling
	Preventive Medicine and Hygienics 06 February 2006
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	Abstract:The 14-3-3 protein is a key player in signal transduction processes in various species of animals and plants. Here, we cloned and expressed the 14-3-3 of Schistosoma japonicum (Sj14-3-3, Chinese mainland strain) in pET28a/E.coli. And the protein can be strongly recognized by polyclonal anti-14-3-3εand sera from animals infected with S. japonicum. Monoclonal antibodies against Sj14-3-3 were raised using a highly specific, affinity-purified protein. The antibodies preparation by ammonium sulfate precipitation was employed for the localization of the native 14-3-3 protein in the parasite by immunofluorescence microscopy. The results demonstrate a wide distribution of this protein in tegument, subtegument, muscle and parenchyma of male, female adult parasites and 15-day-old schistosomulum. The potential use of this protein or its DNA as a vaccine candidate against infection with S. japonicum in BALB/c mice and the recombinant Sj14-3-3 (rSj14-3-3) antigen for diagnosis of schistosomiasis was evaluated. Challenge infection with S. japonicum, immunization with rSj14-3-3, rSjGST, and rSj14-3-3+rSjGST proteins using Freund’s adjuvant or Mycobacterium tuberculosis (Mtb) low molecular peptide as adjuvant respectively, led to 26.0-32.2％ protection, as determined by reduction of adult worm burden; 50.4-58.6％ protection as determined by reduction of the eggs in liver tissue. The mice were directly inoculated with rSj14-3-3 and rSj14-3-3+rSjGST cDNAs through quadriceps femoris muscle for immunization. As the consequence, the worm reduction was found to be 34.20％ in rSj14-3-3 group, 32.40％in rSj14-3-3+rSjGST group; the number of eggs in liver tissue was reduced by 50.74％ and 55.23％, respectively. Indirect ELISA suggested that the rSj14-3-3 antigen is highly sensitive and specific for the diagnosis of schitosomiasis.
	TO cite this article:Liu Qingzhong,Shen Jilong,Li Defa, et al. IMMUNOPROTECTION AND DIAGNOSTIC POTENTIAL OF SIGNALING PROTEIN 14-3-3 OF SCHISTOSOMA JAPONICUM×[OL].[ 6 February 2006] http://en.paper.edu.cn/en_releasepaper/content/5157


	7. Microfluid temperature control for Polymerase Chain Reaction
	Jia Xiaoyu
	Preventive Medicine and Hygienics 26 April 2004
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	Abstract:A fast and continuous DNA amplification reaction can be obtained by moving the sample through three individual temperature zones whose temperature is constant during the course of reaction.In this paper,we present a novel layout of the control system of three temperature zones on the PCR chip.Moreover,the PID control methods used in this system are optimized to improve their reliability and precision.The experimental results have shown that the designed PID controller can significantly improve system performance, and copes well with changes even in the transient process.
	TO cite this article:Jia Xiaoyu. Microfluid temperature control for Polymerase Chain Reaction[OL].[26 April 2004] http://en.paper.edu.cn/en_releasepaper/content/630

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