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Home > Papers > Analytical-Chemistry

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	1. Real-time Refrigeration Turbidimetry for Discrimination of Refined Peanut oils
	Su Rui,Feng Xudong,Yu Aimin,Zhang Hanqi,Wang Xinghua
	Chemistry 12 December 2010
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	Abstract:In this paper, real-time refrigeration turbidimetry and common multivariate analysis techniques, including principal component analysis (PCA) and partial least squares (PLS), were applied for discrimination and purity assessment of the refined peanut oil (RPO) adulterated with other less expensive seed oils. A special turbidimeter was developed. The curves of turbidity vs. refrigeration time for 182 samples were constructed and analyzed by PCA and PLS methods. Discrimination of a certain pure RPO and its adulterated samples was achieved successfully by calculating and comparing the Euclidean or Minkowski distance of their first two principal components obtained with PCA. The PLS method was used to assess the purity of all samples. 46 real RPO samples were determinated using the methods mentioned above and gas chromatography (GC) method. The results indicated that real-time refrigeration turbidimetry and multivariate analysis techniques could discriminate and assess the purity of RPO samples efficiently and rapidly.
	TO cite this article:Su Rui,Feng Xudong,Yu Aimin, et al. Real-time Refrigeration Turbidimetry for Discrimination of Refined Peanut oils[OL].[12 December 2010] http://en.paper.edu.cn/en_releasepaper/content/4397182


	2. Interaction between Ginsenoside Rb1, Rg1 or Re and bovine serum albumin: Fluorescence study
	Chen Rui,Lin Hui,Zhu Ling,Zhang Haixia
	Chemistry 23 November 2010
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	Abstract:In this work, the interactions of the single ingredient (Ginsenoside Rb1, Rg1 and Re) and the multi-ingredients (Rb1 plus Rg1, Rb1 plus Re, Rg1 plus Re) with bovine serum albumin (BSA) were investigated by fluorescence spectroscopy. They were found to quench the intrinsic fluorescence of BSA via static quenching and the hydrophobic and electrostatic forces might play a major role in these reactions. With the gradual adding of Rb1, Rg1 or Re to BSA solution, the fluorescence intensity of BSA decreased by 13.3%, 6.5% and 13.0%, respectively. Further studies show no any two-drug combinations exiting synergistic effect or antagonism, thus the binary mixture had an additive effect on BSA quenching.
	TO cite this article:Chen Rui,Lin Hui,Zhu Ling, et al. Interaction between Ginsenoside Rb1, Rg1 or Re and bovine serum albumin: Fluorescence study[OL].[23 November 2010] http://en.paper.edu.cn/en_releasepaper/content/4392924

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	3. Rapid and Sensitive Visual Detection of Methyl Parathion in Homogeneous Reaction Using Water Soluable Films with Immobilized Cholinesterase
	Ma Qianqian,Liu Li,He Jingjing,Miao Yuqing
	Chemistry 21 October 2010
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	Abstract:A rapid and sensitive visual detection of methyl parathion in homogeneous reaction was developed using water soluable poly(vinyl alcohol) (PVA) to immobilize cholinesterase (ChE). When used, a piece of ChE-PVA film is put into the solution and dissolved quickly. The released ChE from enzyme film is mixed and interacted fully with substrates and methyl parathion inhibitors. The color reaction is developed quickly in homogeneous system and the change or diference of color could be observed easily and clearly, while for common colorimetric sticks or strips for pesticides, it is dificult to discern the diference of developed color exactly due to the uneven dying on the surface of supporting matrix. With 10 min of inhibiton and 5 min enzyme reaction, 0.1 ?g/mL concentration of methyl parathion could be observed by visual detection.
	TO cite this article:Ma Qianqian,Liu Li,He Jingjing, et al. Rapid and Sensitive Visual Detection of Methyl Parathion in Homogeneous Reaction Using Water Soluable Films with Immobilized Cholinesterase[OL].[21 October 2010] http://en.paper.edu.cn/en_releasepaper/content/4389046


	4. Interaction of tamoxifen citrate with salmon sperm DNA and bovine serum albumin
	Zhu Ling,Liu Xiaoyan,Zhang Haixia
	Chemistry 21 July 2010
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	Abstract:Interaction of tamoxifen citrate (TC) with salmon sperm DNA and bovine serum albumin (BSA) were investigated by various spectroscopic analysis methods under simulative physiological conditions. Thermodynamic parameters showed that the interaction process was spontaneous and hydrophobic forces play a major role in the binding between BSA and TC. The quenching constant KSV, the bimolecular quenching constant kq and the numbers of binding sites n (about 1) of both DNA and BSA to TC were similar. The value of n approximately equal to 1 indicated that there is only one type of binding site for DNA or BSA to TC. However, the binding constant (K) of DNA to TC was lower than that of BSA.
	TO cite this article:Zhu Ling,Liu Xiaoyan,Zhang Haixia. Interaction of tamoxifen citrate with salmon sperm DNA and bovine serum albumin[J].


	5. Nanoliter Droplet Array for Quantification of MicroRNA by Two-Step Real Time Reverse Transcription Polymerase Chain Reaction
	Zhang Yunxia,Zhu Ying,Yao Bo,Fang Qun
	Chemistry 15 July 2010
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	Abstract:In the past decade, great effort has been put into the miniaturization of genetic tests such as PCR, however, current parallel micro-PCR devices including droplet based PCR chip and emulsion PCR system are usually designed for performing multiple PCR reactions in identical conditions with the same primers and templates, thus limiting their application for singleplex assays. In this paper, we developed a 6×6 nanoliter droplet array to perform reliable and parallel real time RT-PCR which is generally applied for quantitative biology. Multi-step reagent addition and parallel PCR assay could be simply performed on this droplet array with a traditional pipette. The total reaction volume is only 500nL which is reduced more than 70-fold compared to conventional RT-PCR assay. The total RNA input was as low as 3pg per reaction, which showed a great potential for gene quantification at single cell level. We successfully applied the platform to quantitative measurement of mir-122 in five cultured cell lines and this droplet based PCR chip would be a universal platform for not only for microRNA and other small non-coding RNA, also for mRNA or even DNA quantification.
	TO cite this article:Zhang Yunxia,Zhu Ying,Yao Bo, et al. Nanoliter Droplet Array for Quantification of MicroRNA by Two-Step Real Time Reverse Transcription Polymerase Chain Reaction[J].


	6. Simultaneous separation and validation of benzoic acid compounds by capillary electrophoresis
	Gao Suya ,Li Ting ,Li Hua
	Chemistry 19 March 2010
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	Abstract:A simple and inexpensive capillary electrophoresis (CE) was applied to separate six benzoic acid compounds with similar structure. The investigation was carried out by capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MECC). To avoid a time-consuming and tedious procedure, orthogonal experimental design for separation experiments was applied to find the optimal conditions in terms of the resolution and analytical time. The best conditions for separation were obtained using a 20 mM borax and 30 mM sodium dodecyl sulfate (SDS) buffer (pH9.8) containing 2 mM β-CD and 4% methanol (volume fraction). Online UV detection was performed at 250 nm. A voltage of 16 kV was applied and the temperature was controlled at 21℃. Gravity injection was performed for 5 s. The method was validated for the quantification of benzoic acid and salicylic acid in Zuguangsan powder, a traditional Chinese patent medicine. The separation and determination were satisfactory, quick and reliable.
	TO cite this article:Gao Suya ,Li Ting ,Li Hua . Simultaneous separation and validation of benzoic acid compounds by capillary electrophoresis[OL].[19 March 2010] http://en.paper.edu.cn/en_releasepaper/content/40889


	7. A Series Piezoelectric Quartz Crystal Sensor
	Haiyan Duan,Jing Zhao,Wanzhi Wei
	Chemistry 24 December 2009
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	Abstract:A novel monitoring technique series piezoelectric quartz crystal (SPQC) sensing technique is used to monitor conductivity in unfolding and refolding processes of lysozyme in real time. The lysozyme is unfolded by sodium dodecylbenzene sulfonate (SDBS) and then refolded by cetyltrimethylammonium bromide (CTAB). During the unfolding and refolding process, the conductivity of the solution changed accordingly. The change in conductivity caused a sensitive response of the SPQC sensor. The obtained experimental results indicate that the SPQC sensing technique would be a useful and convenient tool for monitoring the interaction between ionic surfactants and protein.
	TO cite this article:Haiyan Duan,Jing Zhao,Wanzhi Wei. A Series Piezoelectric Quartz Crystal Sensor[OL].[24 December 2009] http://en.paper.edu.cn/en_releasepaper/content/38018


	8. One-Step Electrochemical Deposition of Prussian Blue-Multiwalled Carbon Nanotube Nanocomposite Thin-film
	Dan Du,Minghui Wang
	Chemistry 11 August 2009
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	Abstract:Prussian blue-multiwalled carbon nanotube-modified gold (PB-MWCNT/Au) electrodes were successfully fabricated using the electrochemical co-deposition method in which the MWCNTs not only act as a carrier of PB, but also as a modifier for a catalytic function. Fourier transform infrared proved that PB assembled on the surface of MWCNTs through electrochemical co-deposition. Scanning electron microscopy images showed that a relatively porous PB-MWCNT film was formed. Cyclic voltammetry and electrochemical impedance spectroscopy revealed that a PB-coated MWCNTs composite film improved electron and ion transfer relative to pure PB films and also exhibited larger electrode-specific capacitance than PB alone. Compared with PB film, the PB-MWCNT composite film showed a larger response current to the reduction of H2O2 because of the synergistic effects between the MWCNTs and PB particles. This fast, sensitive, and efficient sensor for H2O2 was observed with a detection sensitivity of 856 μA mM-1 cm-2, and the linear range spanned the concentration of H2O2 from 1 μM to 5 mM. The detection limit was 23 nM at a signal-to-noise ratio of 3. The method presented here demonstrated the great potential for CNTs and other inorganic or metal nanoparticles for constructing bioelectronic devices and biosensors.
	TO cite this article:Dan Du,Minghui Wang. One-Step Electrochemical Deposition of Prussian Blue-Multiwalled Carbon Nanotube Nanocomposite Thin-film [J].J. Mater. Chem.，2010，20（8）：1532-1537.


	9. Kinetic Study and Mathematical Modelling of Chromium(VI) Reduction and Microorganism Growth under Mixed Culture
	Bo Kong,Xiandong Zeng,Xiaoying Liu,Xuefang Li,Jun Li,Shenglian Luo,Wanzhi Wei
	Chemistry 13 July 2009
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	Abstract:The kinetics of chromium (VI) reduction by Pseudomonas aeruginosa (P. aeruginosa) and Escherichia coli (E. coli) was studied under both pure and mixed cultures. Initially, the study of kinetics was performed in pure culture. It was observed that the growth of the two bacteria was both inhibited in the presence of chromium (VI). The maximum specific growth rate (μm) of P. aeruginosa decreased from 2.3942 h−1 (without Cr(VI)) to 1.8551 h−1 (with Cr(VI)). Under the mixed culture, the growth of E. coli was inhibited by P. aeruginosa. The maximum specific growth rate (μm) of E. coli decreased form 0.871 h−1 (in pure culture) to 0.153 h−1 (in mixed culture). When the concentration of each bacterium was 4.5×108 cells mL-1, the half-velocity reduction rate constant (KC) and the maximum specific reduction rate constant (vmax) of chromium (VI) were 80.05 mg chromium (VI) L-1 and 3.674 mg chromium (VI) cells-1 h-1, respectively. The results showed that the simulation appeared in good agreement with the experimental data, supporting the series of mathematical models represented the bacteria growth and chromium (VI) reduction in both pure and mixed cultures usefully.
	TO cite this article:Bo Kong,Xiandong Zeng,Xiaoying Liu, et al. Kinetic Study and Mathematical Modelling of Chromium(VI) Reduction and Microorganism Growth under Mixed Culture[J].


	10. Study on the Determination of Three Components in Potato Using Near Infrared Spectroscopy Based on Partial least squares and Generalized Regression Neural Networks Model
	Qin Huajun,Liu Boping ,Cao Shuwen
	Chemistry 16 April 2009
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	Abstract:Partial least squares (PLS) and generalized regression neural networks (GRNN) prediction model for fibre, starch and protein in potato had been established with good veracity. 12 peak value data from 3 principal components straight ahead compressed from original data by PLS were taken as inputs of GRNN while 3 predictive targets as outputs. 0.1 was chosen as smoothing factor for its good approximation and prediction with the lowest error compared with 0.2, 0.3, 0.4, and 0.5. Predictive correlation coefficient of three components by the model are 0.945, 0.992, and 0.938. The results show that PLS-GRNN using in NIRS is a rapid, effective means for measuring fibre, starch and protein in potato. The results are important in quality controlling and evaluating in fruit and vegetable industry, and can also be used in quantitative analysis of other samples.
	TO cite this article:Qin Huajun,Liu Boping ,Cao Shuwen. Study on the Determination of Three Components in Potato Using Near Infrared Spectroscopy Based on Partial least squares and Generalized Regression Neural Networks Model[OL].[16 April 2009] http://en.paper.edu.cn/en_releasepaper/content/31456

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